FastQCFastQC Report
Wed 25 May 2016
SRR1033013_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033013_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201722
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24450.034.0410461
TCAACGC23900.032.459014
ATCAACG24650.031.662153
GGTATCA17250.031.6211321
CAACGCA25200.030.9718155
AACGCAG25400.030.9046176
GTACATG58550.027.3864671
TACATGG59150.026.6280442
ACATGGG56950.026.1706663
TATCAAC31050.025.8930842
ACGCAGA31850.023.9087077
CGCAGAG33950.021.7523758
CATGGGG44450.021.0489584
TACACCG2501.0686563E-820.6876815
ACACCGT3202.0008883E-1120.5645246
GAGTACA40350.019.4617311
GTATAGA10450.017.9991591
GCAGAGT40000.017.876399
TAGACAG11350.016.9842785
AGTACAT42450.016.392052
Produced by FastQC (version 0.11.5)