##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033013_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2201722
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.433428016797762	33.0	31.0	34.0	30.0	34.0
2	31.463116142728282	33.0	31.0	34.0	28.0	34.0
3	31.66728042868264	34.0	31.0	34.0	30.0	34.0
4	35.02925755386011	37.0	35.0	37.0	33.0	37.0
5	35.124176440077356	37.0	35.0	37.0	33.0	37.0
6	35.130514660797324	37.0	35.0	37.0	33.0	37.0
7	35.06500366531288	37.0	35.0	37.0	33.0	37.0
8	35.1047820751212	37.0	35.0	37.0	33.0	37.0
9	36.725536193942744	39.0	38.0	39.0	33.0	39.0
10-11	36.71837202880291	39.0	38.0	39.0	33.0	39.0
12-13	36.63756800358992	39.0	37.5	39.0	33.0	39.0
14-15	38.01226880596188	41.0	38.0	41.0	33.0	41.0
16-17	38.00556973132848	40.5	38.0	41.0	33.0	41.0
18-19	37.9890111013107	40.0	38.5	41.0	33.0	41.0
20-21	38.01504299816234	40.0	38.5	41.0	33.0	41.0
22-23	37.96991899976473	40.0	38.0	41.0	33.0	41.0
24-25	37.9812919160548	40.0	38.0	41.0	33.0	41.0
26-27	37.89782838160313	40.0	38.0	41.0	33.0	41.0
28-29	37.90600243809163	40.0	38.0	41.0	33.0	41.0
30-31	38.051382281686784	40.0	38.0	41.0	33.0	41.0
32-33	38.04651563639733	40.0	38.0	41.0	33.0	41.0
34-35	37.99842872987598	40.0	38.0	41.0	33.0	41.0
36-37	37.91633798454119	40.0	38.0	41.0	33.0	41.0
38-39	37.82139207402206	40.0	38.0	41.0	32.5	41.0
40-41	37.73969147785233	40.0	38.0	41.0	32.0	41.0
42-43	37.604101017294646	40.0	38.0	41.0	32.0	41.0
44-45	37.43930273667611	40.0	38.0	41.0	31.5	41.0
46-47	37.295333379963495	40.0	37.5	41.0	31.0	41.0
48-49	37.170891692956694	40.0	37.0	41.0	31.0	41.0
50-51	37.039012645556525	40.0	37.0	41.0	31.0	41.0
52-53	36.87655276188365	40.0	36.5	41.0	30.0	41.0
54-55	36.71195818545665	40.0	36.0	41.0	30.0	41.0
56-57	36.53293013377711	40.0	36.0	41.0	30.0	41.0
58-59	36.33625566715507	39.0	35.0	41.0	30.0	41.0
60-61	36.10871013688377	39.0	35.0	41.0	30.0	41.0
62-63	35.85615963323254	39.0	35.0	41.0	29.5	41.0
64-65	35.57735854026984	38.0	35.0	40.0	29.0	41.0
66-67	35.2780335119511	37.5	35.0	40.0	29.0	41.0
68-69	34.95927369577086	37.0	34.0	40.0	28.5	41.0
70-71	34.62614875992519	37.0	34.0	39.5	28.0	41.0
72-73	34.27493298427322	36.0	34.0	39.0	28.0	41.0
74-75	33.91704243315005	36.0	34.0	39.0	28.0	40.5
76-77	33.56114236947262	35.0	34.0	38.0	27.0	40.0
78-79	33.17992666649104	35.0	33.0	37.0	26.5	39.0
80-81	32.835037075525435	35.0	33.0	37.0	26.0	39.0
82-83	32.62174652385723	35.0	33.0	36.0	26.0	38.5
84-85	32.40368084617404	35.0	33.0	36.0	26.0	37.0
86-87	32.237967372810914	35.0	33.0	36.0	26.0	37.0
88-89	32.02742557870612	35.0	33.0	35.0	26.0	37.0
90-91	31.824313650860553	35.0	33.0	35.0	26.0	36.0
92-93	31.605893932113137	35.0	33.0	35.0	25.0	36.0
94-95	31.40424585846896	35.0	33.0	35.0	25.0	36.0
96-97	31.228887434471744	35.0	33.0	35.0	24.5	35.5
98-99	31.051334818837255	35.0	33.0	35.0	24.0	35.0
100	30.91187307026046	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.016624613152401935
1101	2	0.02237895916767485
1101	3	0.08261710660309873
1101	4	-0.04648197245047925
1101	5	0.007414129437542272
1101	6	0.05255084007389854
1101	7	0.07614730424354832
1101	8	0.18977818374229827
1101	9	0.17554738887326948
1101	10-11	0.24496198006980308
1101	12-13	0.19354048065456908
1101	14-15	0.24658086652460298
1101	16-17	0.29081833735357776
1101	18-19	0.2520576445196667
1101	20-21	0.240692157942469
1101	22-23	0.20225152892564324
1101	24-25	0.23943099529746803
1101	26-27	0.10169608428432042
1101	28-29	0.11770528617627463
1101	30-31	0.266303775614098
1101	32-33	0.18656544713132917
1101	34-35	0.17594482352206597
1101	36-37	0.2102800400651148
1101	38-39	0.2495407215190042
1101	40-41	0.3432332268199261
1101	42-43	0.32020620614971307
1101	44-45	0.28650136694401596
1101	46-47	0.2000125114943927
1101	48-49	0.22888152855475852
1101	50-51	0.25998087459319663
1101	52-53	0.24708385757165274
1101	54-55	0.15379475644613194
1101	56-57	0.29707615514437435
1101	58-59	0.2512893577668365
1101	60-61	0.303739469250381
1101	62-63	0.24119755031202317
1101	64-65	0.3229697149317161
1101	66-67	0.3075644000542539
1101	68-69	0.33596396225915726
1101	70-71	0.2597126977925015
1101	72-73	0.302498355974933
1101	74-75	0.2923483224184338
1101	76-77	0.23793849197148376
1101	78-79	0.15690234788177548
1101	80-81	0.15349870911047248
1101	82-83	0.1560505660844882
1101	84-85	0.14964811241781462
1101	86-87	0.1353724798723519
1101	88-89	0.18550423468301602
1101	90-91	0.2051013326600959
1101	92-93	0.21174788523221366
1101	94-95	0.2296137878227924
1101	96-97	0.2583855715721519
1101	98-99	0.2723986909951037
1101	100	0.2582719155664961
1102	1	-0.24139857249328855
1102	2	-0.21387192929102739
1102	3	-0.14563321979036914
1102	4	-0.2549240467622269
1102	5	-0.23934728796729132
1102	6	-0.19206827205402277
1102	7	-0.20385440559891777
1102	8	-0.05895955689187815
1102	9	-0.18511409278707447
1102	10-11	-0.20236668666689184
1102	12-13	-0.17068313490736386
1102	14-15	-0.1295225161895246
1102	16-17	-0.07879389675114368
1102	18-19	-0.12258725621624222
1102	20-21	-0.07964500843930011
1102	22-23	-0.07509799243214843
1102	24-25	-0.036834526866137196
1102	26-27	-0.18330600527050223
1102	28-29	-0.138906256260789
1102	30-31	-0.020120757351072882
1102	32-33	-0.07127925097006482
1102	34-35	-0.0011286901541751604
1102	36-37	-0.031662441016337084
1102	38-39	-0.014006142034205027
1102	40-41	-0.10553527733621593
1102	42-43	0.03462693107744741
1102	44-45	-0.05906734439410144
1102	46-47	-0.03582899247576421
1102	48-49	-0.04858213388232002
1102	50-51	0.06521731260935582
1102	52-53	0.05537788953934353
1102	54-55	0.13802964514493965
1102	56-57	0.14494781054927586
1102	58-59	0.08754056454726822
1102	60-61	0.11969630118513663
1102	62-63	0.10983947155649787
1102	64-65	0.1245914959600114
1102	66-67	0.12354817565016418
1102	68-69	0.08667552502333109
1102	70-71	0.015838003779265364
1102	72-73	0.11269640666357361
1102	74-75	-0.036410500209512975
1102	76-77	-0.0889416639879812
1102	78-79	-0.1075917502058843
1102	80-81	0.03112128230514344
1102	82-83	-0.0437946613404776
1102	84-85	0.01370199428315999
1102	86-87	-0.02946406419042802
1102	88-89	-0.06749492449285555
1102	90-91	-0.06878291555264582
1102	92-93	-0.0029330677139220995
1102	94-95	0.013580081189012105
1102	96-97	-0.01985331797611778
1102	98-99	0.06555265912564323
1102	100	0.01986894480616641
1103	1	-0.09542108706324726
1103	2	0.013177854117788712
1103	3	-0.034780954163853295
1103	4	-0.02944039099171647
1103	5	-0.04319959231794712
1103	6	0.0037584092339386643
1103	7	0.013329741792219352
1103	8	0.011130925593363372
1103	9	0.17349745299278396
1103	10-11	0.1205960932690715
1103	12-13	0.19738821344976287
1103	14-15	0.195749740952877
1103	16-17	0.1856920138207272
1103	18-19	0.1653874886004445
1103	20-21	0.15871068515414066
1103	22-23	0.17526130602715284
1103	24-25	0.09338509184185284
1103	26-27	0.10510372610714569
1103	28-29	0.06302552851745702
1103	30-31	0.1285405753579809
1103	32-33	0.07673796279249956
1103	34-35	0.03819072859640471
1103	36-37	0.11124439573146105
1103	38-39	0.14348385569515187
1103	40-41	0.15292473822385233
1103	42-43	0.07306460544610616
1103	44-45	0.14337456498788015
1103	46-47	0.20617502163391066
1103	48-49	0.14286706884033862
1103	50-51	0.11900382991817082
1103	52-53	0.248532054761192
1103	54-55	0.1443545521629943
1103	56-57	0.19598304336059158
1103	58-59	0.20477483330338941
1103	60-61	0.14000359487785374
1103	62-63	0.14435280586380372
1103	64-65	0.15652989464621214
1103	66-67	0.09782401213569614
1103	68-69	0.11379243761765423
1103	70-71	0.11147432069062546
1103	72-73	0.041472362841929566
1103	74-75	0.06747273945245524
1103	76-77	0.08100980761754073
1103	78-79	0.12842267277795116
1103	80-81	0.11068982932241767
1103	82-83	0.08645785088842928
1103	84-85	0.1417453600450358
1103	86-87	0.027844260092606987
1103	88-89	0.07051121768460433
1103	90-91	0.14137334957397485
1103	92-93	0.1972147661102106
1103	94-95	0.20124674902913853
1103	96-97	0.1874046869207291
1103	98-99	0.11208169959413539
1103	100	0.11960650071414847
1104	1	-0.11849785814409941
1104	2	-0.053690359338506966
1104	3	0.05491199178259265
1104	4	-0.032945808455068004
1104	5	-0.05760194764906146
1104	6	0.08683276713462362
1104	7	0.02863094367366159
1104	8	0.141449171085263
1104	9	0.1820121111704367
1104	10-11	0.15703458828547667
1104	12-13	0.20251191205617403
1104	14-15	0.20503459879387265
1104	16-17	0.1461806029259307
1104	18-19	0.18909046732241563
1104	20-21	0.20446462317829628
1104	22-23	0.19745772880286694
1104	24-25	0.05032874450534308
1104	26-27	0.02636447400801245
1104	28-29	0.04240072085716662
1104	30-31	0.0865766755242916
1104	32-33	0.07220242430017265
1104	34-35	0.1637319073718615
1104	36-37	0.16197057797954528
1104	38-39	0.11005521435762944
1104	40-41	0.1891082517469087
1104	42-43	0.16389645471568315
1104	44-45	0.10320856846218618
1104	46-47	0.09737559558400477
1104	48-49	0.06754988699753994
1104	50-51	0.04447818378287849
1104	52-53	0.05054228849492404
1104	54-55	0.09303842303850729
1104	56-57	0.04337914208068128
1104	58-59	0.06719791844072631
1104	60-61	0.19646906790321594
1104	62-63	0.17158936983157957
1104	64-65	0.14213931092567833
1104	66-67	0.0647303361812277
1104	68-69	0.1506408369308616
1104	70-71	0.0680509513529799
1104	72-73	0.05841696564256438
1104	74-75	0.21515810448169503
1104	76-77	0.04866940963619726
1104	78-79	0.03934899763068245
1104	80-81	0.010587941828738678
1104	82-83	0.05161630097091319
1104	84-85	0.10093297597437356
1104	86-87	0.01967024289596253
1104	88-89	0.0937247286347258
1104	90-91	0.14560177515469164
1104	92-93	0.09090482548958079
1104	94-95	0.07153894562848961
1104	96-97	0.1528014070136905
1104	98-99	0.11521568070238075
1104	100	0.1638778464278552
1105	1	0.0555247987270171
1105	2	0.06903716780297486
1105	3	0.07148456075496767
1105	4	0.10527789670107524
1105	5	0.10266836279809866
1105	6	0.10985014045180463
1105	7	0.1772294688043985
1105	8	0.18755553668745506
1105	9	0.24189369311908848
1105	10-11	0.2703780746607194
1105	12-13	0.22378470148256469
1105	14-15	0.16985223222631873
1105	16-17	0.26169944021907554
1105	18-19	0.24530473683000764
1105	20-21	0.24740982383478638
1105	22-23	0.19446357447542084
1105	24-25	0.17393435630285836
1105	26-27	0.18317029211629432
1105	28-29	0.16211333018893725
1105	30-31	0.3385108390358127
1105	32-33	0.24290066710773317
1105	34-35	0.19198032408110066
1105	36-37	0.2668807804325155
1105	38-39	0.28809379952792824
1105	40-41	0.25930657610379626
1105	42-43	0.2873922307011796
1105	44-45	0.20491081290434465
1105	46-47	0.19362215982953046
1105	48-49	0.1765687011198409
1105	50-51	0.19127916309702897
1105	52-53	0.2443248593269871
1105	54-55	0.21585652710989933
1105	56-57	0.26508287908620076
1105	58-59	0.19803900196950508
1105	60-61	0.19032653773146535
1105	62-63	0.1726841169112987
1105	64-65	0.21049720836129637
1105	66-67	0.22630212577072228
1105	68-69	0.28851788220910635
1105	70-71	0.3064100103399312
1105	72-73	0.24830081652826408
1105	74-75	0.2742770046069367
1105	76-77	0.25015387825054347
1105	78-79	0.29682821458860786
1105	80-81	0.32228181467558414
1105	82-83	0.2970761699744102
1105	84-85	0.3062293491055854
1105	86-87	0.29517472028513225
1105	88-89	0.3003215276881477
1105	90-91	0.24177356077203527
1105	92-93	0.3127026000053803
1105	94-95	0.3256109955138058
1105	96-97	0.4151966040892283
1105	98-99	0.36326797578937686
1105	100	0.4280079979531273
1106	1	-0.2196699748393769
1106	2	-0.16467043405251403
1106	3	-0.16133935153431977
1106	4	-0.17937956374110797
1106	5	-0.2147950317186016
1106	6	-0.0826106693375337
1106	7	-0.10306296150561423
1106	8	-0.12120766552553164
1106	9	-0.09247812623029716
1106	10-11	-0.14233812342359897
1106	12-13	-0.10773788673704843
1106	14-15	-0.1245723385366233
1106	16-17	-0.04149094620340321
1106	18-19	-0.010571784425643216
1106	20-21	0.011966146767164787
1106	22-23	-0.08494732020784568
1106	24-25	-0.09414179091402275
1106	26-27	-0.08243198382229622
1106	28-29	-0.021307682840387088
1106	30-31	0.005354559526843161
1106	32-33	-0.013881801088629686
1106	34-35	-0.002928653308849505
1106	36-37	-0.01893234033283875
1106	38-39	0.03633561053726453
1106	40-41	0.05106557497570208
1106	42-43	-0.027573599203080335
1106	44-45	-0.06478222487402974
1106	46-47	-0.07310992915861902
1106	48-49	-0.1434238491215183
1106	50-51	-0.1348022868316292
1106	52-53	-0.16579541090913352
1106	54-55	-0.12652091086238215
1106	56-57	-0.11589143102134614
1106	58-59	-0.1430864227873485
1106	60-61	-0.19002356071792548
1106	62-63	-0.1990029805892135
1106	64-65	-0.24914011992665053
1106	66-67	-0.09181376880916048
1106	68-69	-0.11509777802216803
1106	70-71	-0.14509389891661328
1106	72-73	-0.20085802703025024
1106	74-75	-0.16683615705454713
1106	76-77	-0.0963846835841764
1106	78-79	-0.1842362163463349
1106	80-81	-0.1597149848575654
1106	82-83	-0.16026606401119636
1106	84-85	-0.18025669106911835
1106	86-87	-0.1829807435987547
1106	88-89	-0.1326602252854343
1106	90-91	-0.14241787332660394
1106	92-93	-0.11991829630914097
1106	94-95	-0.14376974976073598
1106	96-97	-0.13679533041262815
1106	98-99	-0.26944398908590017
1106	100	-0.2728600683721254
1107	1	-0.08468623132582209
1107	2	-0.015636133942702912
1107	3	-0.01947674575949776
1107	4	0.049025685704449984
1107	5	0.020206475505311516
1107	6	0.06136420308672541
1107	7	0.046266323854936786
1107	8	0.0707959733436141
1107	9	0.10205926368853113
1107	10-11	0.07699713700316124
1107	12-13	0.12384222221370322
1107	14-15	0.0880151342795159
1107	16-17	0.08460313686615706
1107	18-19	0.13278889425575358
1107	20-21	0.17103371346183138
1107	22-23	0.10702106038772286
1107	24-25	-0.011303149999356776
1107	26-27	0.02895024816098868
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2216	24-25	-0.4133161076336833
2216	26-27	-0.4712555176243356
2216	28-29	-0.3216744002586225
2216	30-31	-0.5465932932338902
2216	32-33	-0.5861339393717344
2216	34-35	-0.6138847606633462
2216	36-37	-0.7107629047201627
2216	38-39	-0.5821770698920403
2216	40-41	-0.5660919265543001
2216	42-43	-0.6035770249341255
2216	44-45	-0.4446301940648425
2216	46-47	-0.43361393704003603
2216	48-49	-0.5557060651739363
2216	50-51	-0.4636932903693136
2216	52-53	-0.46035308857694446
2216	54-55	-0.47829113312441507
2216	56-57	-0.5264616771664663
2216	58-59	-0.47747001510296627
2216	60-61	-0.481359371922089
2216	62-63	-0.4911201047663667
2216	64-65	-0.4797160383061865
2216	66-67	-0.5711745229178007
2216	68-69	-0.486967189751752
2216	70-71	-0.45226412565671836
2216	72-73	-0.505647519962352
2216	74-75	-0.5740252290471446
2216	76-77	-0.4727568045520414
2216	78-79	-0.444563228123684
2216	80-81	-0.42641949129853174
2216	82-83	-0.6209841987416915
2216	84-85	-0.4378570000566242
2216	86-87	-0.3434209761603668
2216	88-89	-0.45339828747103894
2216	90-91	-0.46570302998476265
2216	92-93	-0.6358594767309746
2216	94-95	-0.48796630761006554
2216	96-97	-0.5129095886187081
2216	98-99	-0.5289987186845124
2216	100	-0.5020552827975138
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29450.0
3	5460.0
4	3115.0
5	2582.0
6	2200.0
7	2258.0
8	2749.0
9	3104.0
10	3508.0
11	3726.0
12	4148.0
13	4533.0
14	4895.0
15	5190.0
16	5127.0
17	5127.0
18	5019.0
19	5128.0
20	5034.0
21	5376.0
22	6042.0
23	8203.0
24	12402.0
25	16849.0
26	17489.0
27	15889.0
28	16987.0
29	21231.0
30	25809.0
31	31312.0
32	41140.0
33	55646.0
34	80023.0
35	124782.0
36	225697.0
37	455450.0
38	676120.0
39	262493.0
40	429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.40053883169509	19.453932980535523	13.022174996682296	24.123353191087098
2	15.571338798348044	19.905970000731557	40.28345314653349	24.23923805438691
3	18.350738098689767	25.137846741418006	29.715181115144123	26.7962340447481
4	12.145334381803885	16.53109275782603	38.77806211733477	32.54551074303532
5	12.91890955658629	37.857208774353566	35.681122538276306	13.542759130783837
6	31.2461132264857	37.87408490349892	17.802195504253177	13.077606365762207
7	27.565865620223796	32.13379424341253	22.63543400799925	17.66490612836443
8	25.19187987031144	36.51575890585849	20.30123355079946	17.991127673030608
9	24.837090418738885	17.10935912305021	20.905664975247017	37.147885482963886
10-11	23.967610618141247	26.88673787165688	28.855321016929658	20.29033049327222
12-13	24.56562329564533	24.206809393499245	28.774587891446007	22.452979419409417
14-15	21.880864644210543	26.205927145475115	25.732151987252628	26.18105622306171
16-17	21.772446314729198	29.539915859913187	26.217698327039074	22.46993949831854
18-19	21.74463168131506	28.546513835066715	28.010619679050002	21.698234804568216
20-21	22.930942540691497	27.873022252065205	27.48223476253841	21.71380044470489
22-23	22.427610904207576	27.762828713413484	27.477112480032357	22.33244790234658
24-25	22.34153297585536	27.84300952025311	27.56296466401318	22.25249283987835
26-27	22.114665314918494	27.743137208506546	27.633997801285737	22.508199675289216
28-29	22.134717173562688	27.818583672612345	27.65621605505546	22.390483098769508
30-31	22.375586954757924	28.323326987180348	27.27997307297818	22.021112985083548
32-33	22.008586488382605	27.88540621962475	27.779546649230973	22.326460642761674
34-35	22.010259177916534	27.67867871346647	27.94123425864913	22.369827849967873
36-37	22.484077880352913	28.00916132521114	27.24898945947727	22.257771334958683
38-39	22.391370404823068	28.132911183832988	27.427960002918166	22.04775840842578
40-41	22.071936520289253	27.861082675268335	27.773464419033004	22.29351638540941
42-43	21.99349101264298	27.805375823566443	27.677309679164196	22.523823484626384
44-45	22.17754187646089	27.86580896765902	27.694269166129754	22.26237998975034
46-47	22.19125652010864	28.057865609054854	27.528552109731013	22.22232576110549
48-49	22.14666458789107	27.911016889445655	27.60604141884616	22.336277103817114
50-51	22.131103392839975	27.75480048006505	27.663328134370946	22.45076799272403
52-53	22.219305590024845	27.70335441490237	27.634218868322463	22.44312112675032
54-55	22.25174667849867	28.235982542873018	27.40643950282866	22.105831275799652
56-57	22.002623428874262	27.945267056151014	27.585431879478623	22.4666776354961
58-59	21.94532824934365	27.526559028433482	27.91190326693832	22.616209455284547
60-61	22.145794049328213	27.95639549284355	27.565907369054454	22.331903088773785
62-63	22.059375294723218	28.070331328552616	27.446103290332623	22.424190086391544
64-65	22.02854030233741	27.734419206483608	27.681243600179435	22.555796890999552
66-67	21.954803939213793	27.777383880638258	27.515343808573263	22.752468371574686
68-69	22.30838698652239	27.831211294030382	27.5709444688357	22.289457250611523
70-71	22.214728636900418	27.685800834893463	27.636673414680647	22.462797113525472
72-73	22.091000395377268	27.800339024645183	27.761301200219958	22.34735937975759
74-75	22.074731641297028	27.873083870346143	27.580541696121983	22.47164279223484
76-77	22.070710892160424	27.882697328851613	27.43552531691158	22.611066462076383
78-79	22.22641054147697	27.92987890152731	27.562700388922934	22.281010168072783
80-81	22.20905713242533	27.616372262188303	27.715863988743507	22.45870661664286
82-83	22.027525147467507	27.7497711387893	27.84692780664278	22.37577590710041
84-85	22.061967159109628	27.690266441744622	27.65586927258166	22.59189712656409
86-87	22.1930260005657	27.837746655504148	27.531246911658965	22.43798043227119
88-89	21.927610823661343	27.727578198731262	27.631861742998247	22.712949234609145
90-91	21.88477531361086	27.65827758420415	27.759397307366473	22.697549794818517
92-93	22.175998953103917	27.776847556890328	27.627469480866317	22.41968400913944
94-95	21.986765919994838	27.56949300560288	27.696513092997062	22.74722798140522
96-97	21.94804212265425	27.598490251224177	27.759638870782798	22.69382875533877
98-99	21.877850583583683	27.71443715578582	27.650527284834688	22.75718497579581
100	22.092853640570333	27.543859569399842	27.527909395742896	22.83537739428693
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	357.0
1	344.0
2	313.5
3	268.0
4	260.5
5	266.5
6	258.5
7	246.0
8	217.5
9	211.5
10	194.5
11	171.5
12	181.0
13	193.5
14	219.5
15	271.5
16	344.5
17	455.0
18	634.0
19	927.0
20	1299.0
21	1850.0
22	2833.5
23	4361.0
24	6465.5
25	9020.5
26	12417.5
27	16814.0
28	22111.0
29	28145.5
30	33927.5
31	39423.0
32	44980.0
33	51026.0
34	56952.0
35	62058.0
36	66279.5
37	70102.5
38	73448.0
39	75396.5
40	76150.0
41	76867.0
42	77833.5
43	78341.0
44	77715.5
45	76994.5
46	76180.5
47	73856.0
48	72661.0
49	72011.0
50	70119.5
51	67519.5
52	63622.0
53	60946.0
54	59336.5
55	56615.0
56	52534.0
57	48357.0
58	43830.0
59	38251.0
60	33446.5
61	29040.0
62	25075.5
63	21260.5
64	17390.0
65	14490.0
66	11887.0
67	9509.0
68	7818.0
69	6380.0
70	5134.5
71	3895.0
72	2958.5
73	2377.0
74	1827.5
75	1304.5
76	903.0
77	666.0
78	475.0
79	343.0
80	250.0
81	150.5
82	96.0
83	53.0
84	39.0
85	32.5
86	21.5
87	15.0
88	9.0
89	10.5
90	12.0
91	7.0
92	4.5
93	4.5
94	4.5
95	5.0
96	3.0
97	1.0
98	1.5
99	1.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0638591066447081
2	0.04246676010867857
3	0.05772754235094167
4	0.07684893914853919
5	0.09052005657389989
6	0.08874871577792293
7	0.09156469345357861
8	0.10441826897310377
9	0.09942217954855335
10-11	0.10435014048095083
12-13	0.10866494498397164
14-15	0.10748405111998699
16-17	0.07723500060407262
18-19	0.10085287788376553
20-21	0.09360854821816741
22-23	0.05845424626723992
24-25	0.021233380054339286
26-27	0.1850369846874401
28-29	0.06667508432036379
30-31	0.07728041959884127
32-33	0.07437360393364831
34-35	0.021505894022951125
36-37	0.05300396689500309
38-39	0.07773460954652767
40-41	0.08348011238476066
42-43	0.07662184417469599
44-45	0.07491863187087197
46-47	0.0816179335992464
48-49	0.07762106205960607
50-51	0.07282935811151453
52-53	0.07703061512761375
54-55	0.06697030778635994
56-57	0.070308603901855
58-59	0.06728824074974042
60-61	0.06354117368132763
62-63	0.07105801731553756
64-65	0.06653882733605787
66-67	0.06749262622619931
68-69	0.06722011225758746
70-71	0.060021201586758
72-73	0.05868134124108311
74-75	0.06156544740889176
76-77	0.05570639708373719
78-79	0.052572486444701014
80-81	0.047076788077695546
82-83	0.05234539147085781
84-85	0.04357952548051025
86-87	0.04108148076823504
88-89	0.04319346402497681
90-91	0.03931013997225808
92-93	0.04226237463221969
94-95	0.04037748634932112
96-97	0.04353410648574162
98-99	0.04389745844389074
100	0.05082385514610836
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2201722.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.08896874955863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.68955760682998	57.25536850270376
2	11.657177017164438	16.34079031328223
3	3.16340853054135	6.651601249175997
4	1.281105494891502	3.5916545198535337
5	0.6741242485311879	2.3624336694311068
6	0.3836313106819663	1.6132993767444332
7	0.2550057973062008	1.2511165350845417
8	0.16597891209208004	0.9306632626166033
9	0.11728151568165103	0.7398126438760899
>10	0.5790588135219205	7.076905675105007
>50	0.025499022067413914	1.2042677592210602
>100	0.007977517354662678	0.9078999352120057
>500	1.9421333576000893E-4	0.07418655769350721
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.541899476864018E-5	0.0
5	0.0	0.0	0.0	4.541899476864018E-5	0.0
6	0.0	0.0	0.0	4.541899476864018E-5	0.0
7	0.0	0.0	0.0	4.541899476864018E-5	0.0
8	0.0	0.0	0.0	4.541899476864018E-5	0.0
9	0.0	0.0	0.0	1.8167597907456072E-4	0.0
10-11	0.0	0.0	0.0	1.8167597907456072E-4	0.0
12-13	0.0	0.0	0.0	1.8167597907456072E-4	0.0
14-15	0.0	0.0	0.0	1.8167597907456072E-4	0.0
16-17	0.0	0.0	0.0	1.8167597907456072E-4	0.0
18-19	0.0	0.0	0.0	1.8167597907456072E-4	0.0
20-21	0.0	0.0	0.0	1.8167597907456072E-4	0.0
22-23	0.0	0.0	0.0	2.270949738432009E-4	0.0
24-25	0.0	0.0	0.0	2.270949738432009E-4	0.0
26-27	4.541899476864018E-5	0.0	0.0	2.952234659961612E-4	0.0
28-29	4.541899476864018E-5	0.0	0.0	7.72122911066883E-4	0.0
30-31	4.541899476864018E-5	0.0	0.0	0.003020363152114572	0.0
32-33	4.541899476864018E-5	0.0	0.0	0.009129217948496677	0.0
34-35	4.541899476864018E-5	0.0	0.0	0.016895866053934148	0.0
36-37	4.541899476864018E-5	0.0	0.0	0.031180139908671484	0.0
38-39	4.541899476864018E-5	0.0	0.0	0.056364972507882465	0.0
40-41	4.541899476864018E-5	0.0	0.0	0.09090611802943332	0.0
42-43	4.541899476864018E-5	0.0	0.0	0.12540184455621556	0.0
44-45	4.541899476864018E-5	0.0	0.0	0.16337212418279873	0.0
46-47	4.541899476864018E-5	0.0	0.0	0.2110166496951023	0.0
48-49	4.541899476864018E-5	0.0	0.0	0.26452022553256044	0.0
50-51	4.541899476864018E-5	0.0	0.0	0.3284474606694215	0.0
52-53	4.541899476864018E-5	4.541899476864018E-5	0.0	0.3900583270730819	0.0
54-55	4.541899476864018E-5	4.541899476864018E-5	0.0	0.4562792214457593	0.0
56-57	4.541899476864018E-5	6.812849215296027E-5	0.0	0.5342863449608988	0.0
58-59	4.541899476864018E-5	9.083798953728036E-5	0.0	0.6271454797653837	0.0
60-61	4.541899476864018E-5	9.083798953728036E-5	0.0	0.7236835531461283	0.0
62-63	4.541899476864018E-5	9.083798953728036E-5	0.0	0.822061095815003	0.0
64-65	4.541899476864018E-5	9.083798953728036E-5	0.0	0.9319750631551122	0.0
66-67	4.541899476864018E-5	9.083798953728036E-5	0.0	1.0612375222666621	0.0
68-69	4.541899476864018E-5	9.083798953728036E-5	0.0	1.2110066575162532	0.0
70-71	4.541899476864018E-5	9.083798953728036E-5	0.0	1.3667711000753047	0.0
72-73	4.541899476864018E-5	9.083798953728036E-5	0.0	1.5367971069917092	0.0
74-75	9.083798953728036E-5	9.083798953728036E-5	0.0	1.7059147340127407	0.0
76-77	9.083798953728036E-5	9.083798953728036E-5	0.0	1.887045685150078	0.0
78-79	9.083798953728036E-5	9.083798953728036E-5	0.0	2.0919080610540295	0.0
80-81	9.083798953728036E-5	9.083798953728036E-5	0.0	2.3251118896936127	0.0
82-83	9.083798953728036E-5	9.083798953728036E-5	0.0	2.5731904391199256	0.0
84-85	9.083798953728036E-5	9.083798953728036E-5	0.0	2.8183394633836603	0.0
86-87	9.083798953728036E-5	9.083798953728036E-5	0.0	3.074411755889254	0.0
88	9.083798953728036E-5	9.083798953728036E-5	0.0	3.2945122045380844	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2445	0.0	34.041046	1
TCAACGC	2390	0.0	32.45901	4
ATCAACG	2465	0.0	31.66215	3
GGTATCA	1725	0.0	31.621132	1
CAACGCA	2520	0.0	30.971815	5
AACGCAG	2540	0.0	30.904617	6
GTACATG	5855	0.0	27.386467	1
TACATGG	5915	0.0	26.628044	2
ACATGGG	5695	0.0	26.170666	3
TATCAAC	3105	0.0	25.893084	2
ACGCAGA	3185	0.0	23.908707	7
CGCAGAG	3395	0.0	21.752375	8
CATGGGG	4445	0.0	21.048958	4
TACACCG	250	1.0686563E-8	20.687681	5
ACACCGT	320	2.0008883E-11	20.564524	6
GAGTACA	4035	0.0	19.461731	1
GTATAGA	1045	0.0	17.999159	1
GCAGAGT	4000	0.0	17.87639	9
TAGACAG	1135	0.0	16.984278	5
AGTACAT	4245	0.0	16.39205	2
>>END_MODULE