FastQCFastQC Report
Wed 25 May 2016
SRR1033013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033013_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201722
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37180.1688678225498042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31820.14452324135381306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31200.051.9701961
GTATCAA53450.040.1249241
TCAACGC64700.032.6187824
ATCAACG64750.032.5213
CAACGCA66700.031.5680855
TATCAAC68050.030.9493752
AACGCAG68750.030.6951476
ACGCAGA81000.025.8783197
CGCAGAG83350.025.0928788
GTACATG61650.024.6191641
TACATGG62250.024.1448122
ACATGGG57850.023.968693
GCAGAGT92750.022.4474079
GAGTACT64900.020.16795212-13
AGAGTAC86750.019.77423710-11
GTGGTAT15600.019.3377481
CAGAGTA93600.019.30620210-11
CATGGGG42800.019.2185214
GAGTACA44250.019.0673481
AGTACTT67600.018.9105212-13
Produced by FastQC (version 0.11.5)