##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033013_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2201722 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.692134156809985 34.0 31.0 34.0 31.0 34.0 2 32.69514316521341 34.0 31.0 34.0 31.0 34.0 3 32.79702523751863 34.0 31.0 34.0 31.0 34.0 4 36.28716431956441 37.0 37.0 37.0 35.0 37.0 5 36.27318344459473 37.0 37.0 37.0 35.0 37.0 6 36.34269676189819 37.0 37.0 37.0 35.0 37.0 7 36.29291300173228 37.0 37.0 37.0 35.0 37.0 8 36.30218347275451 37.0 37.0 37.0 35.0 37.0 9 38.10201378739005 39.0 39.0 39.0 37.0 39.0 10-11 38.06185385802567 39.0 39.0 39.0 35.0 39.0 12-13 38.040912067917745 39.0 39.0 39.0 35.0 39.0 14-15 39.52567013455831 41.0 39.0 41.0 37.0 41.0 16-17 39.520216221666495 41.0 39.0 41.0 37.0 41.0 18-19 39.53472100474083 41.0 39.5 41.0 37.0 41.0 20-21 39.5256928440557 41.0 39.5 41.0 37.0 41.0 22-23 39.455226182052044 41.0 39.0 41.0 36.0 41.0 24-25 39.39967875145 41.0 39.0 41.0 36.0 41.0 26-27 39.28775998968081 41.0 39.0 41.0 36.0 41.0 28-29 39.233401855456776 41.0 39.0 41.0 36.0 41.0 30-31 39.230696473033376 41.0 39.0 41.0 36.0 41.0 32-33 39.18916693388175 41.0 39.0 41.0 35.0 41.0 34-35 39.10581649272706 41.0 39.0 41.0 35.0 41.0 36-37 39.02214198704469 41.0 39.0 41.0 35.0 41.0 38-39 38.92425814884895 41.0 39.0 41.0 35.0 41.0 40-41 38.82316931928736 41.0 39.0 41.0 35.0 41.0 42-43 38.704127042378644 41.0 38.5 41.0 35.0 41.0 44-45 38.53370021283341 41.0 38.0 41.0 35.0 41.0 46-47 38.329474384141136 40.5 38.0 41.0 34.5 41.0 48-49 38.117467146170135 40.0 38.0 41.0 34.0 41.0 50-51 37.97256124978539 40.0 38.0 41.0 34.0 41.0 52-53 37.832282186397734 40.0 38.0 41.0 33.0 41.0 54-55 37.679388678498015 40.0 37.0 41.0 33.0 41.0 56-57 37.523937399907894 40.0 37.0 41.0 33.0 41.0 58-59 37.33940706410709 40.0 36.5 41.0 33.0 41.0 60-61 37.142396724018745 40.0 36.0 41.0 33.0 41.0 62-63 36.90675934563946 39.0 35.5 41.0 32.0 41.0 64-65 36.6377340100158 39.0 35.0 41.0 32.0 41.0 66-67 36.35703758240141 38.5 35.0 41.0 32.0 41.0 68-69 36.04470228303119 37.5 35.0 40.0 31.0 41.0 70-71 35.71752973354492 37.0 35.0 40.0 31.0 41.0 72-73 35.37210329006114 36.5 35.0 39.0 31.0 41.0 74-75 35.02492072114463 36.0 35.0 39.0 31.0 41.0 76-77 34.679180886596946 36.0 35.0 38.5 30.5 40.0 78-79 34.32424824750809 35.0 34.5 37.0 30.0 39.0 80-81 34.00118975056796 35.0 34.0 37.0 30.0 39.0 82-83 33.705487114177 35.0 34.0 36.5 30.0 39.0 84-85 33.49018223009081 35.0 34.0 36.0 30.0 37.0 86-87 33.286635415370334 35.0 34.0 36.0 30.0 37.0 88-89 33.082352131649685 35.0 34.0 35.5 30.0 37.0 90-91 32.9009802327451 35.0 34.0 35.0 29.0 36.0 92-93 32.72833877301494 35.0 34.0 35.0 29.0 36.0 94-95 32.57394961761749 35.0 34.0 35.0 29.0 36.0 96-97 32.42754489440538 35.0 34.0 35.0 29.0 36.0 98-99 32.29906636714354 35.0 34.0 35.0 29.0 35.5 100 32.19435514565417 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12870628879502277 1101 2 0.12793129191671682 1101 3 0.1412905634667183 1101 4 0.1629569249979923 1101 5 0.09718733714031913 1101 6 0.09392782035179437 1101 7 0.15830006098973826 1101 8 0.20380041300846585 1101 9 0.20228787077935806 1101 10-11 0.26651677304089816 1101 12-13 0.24836722942241352 1101 14-15 0.3478901349254855 1101 16-17 0.35188112794108406 1101 18-19 0.31388320046603724 1101 20-21 0.356205905356596 1101 22-23 0.327805191464563 1101 24-25 0.3344758161791219 1101 26-27 0.34396452945354383 1101 28-29 0.30578177711540633 1101 30-31 0.2704513657241989 1101 32-33 0.30899440089434904 1101 34-35 0.3417637606195356 1101 36-37 0.36336143048266223 1101 38-39 0.26412899888118346 1101 40-41 0.24601771767491698 1101 42-43 0.3268022650025415 1101 44-45 0.33623932064863027 1101 46-47 0.16532775434789215 1101 48-49 0.24441037583891045 1101 50-51 0.2446753315513419 1101 52-53 0.20301693479710536 1101 54-55 0.26627181510287556 1101 56-57 0.2642033307853211 1101 58-59 0.24164029883099403 1101 60-61 0.23514345731652497 1101 62-63 0.24530256181163423 1101 64-65 0.2553442898917808 1101 66-67 0.32106019437222955 1101 68-69 0.3210186211403112 1101 70-71 0.23990266665489202 1101 72-73 0.22330102010838715 1101 74-75 0.2161509227191445 1101 76-77 0.28952402837429503 1101 78-79 0.18965901901704285 1101 80-81 0.23629729696193635 1101 82-83 0.2588760843165261 1101 84-85 0.2920094615682842 1101 86-87 0.25465004017815573 1101 88-89 0.29562270952218483 1101 90-91 0.3047535764008913 1101 92-93 0.23912508419575573 1101 94-95 0.24621270757301517 1101 96-97 0.22828627857718686 1101 98-99 0.14017406409605826 1101 100 0.1416668394038183 1102 1 0.07192701354085074 1102 2 0.05581573021299846 1102 3 0.03869705986763705 1102 4 0.046664672903546034 1102 5 0.09833420487718314 1102 6 0.14084730723534733 1102 7 0.10873350150654915 1102 8 0.0795492080470126 1102 9 0.15804660655094693 1102 10-11 0.15458382342168164 1102 12-13 0.15961196745045214 1102 14-15 0.18253756859292025 1102 16-17 0.23797196877885796 1102 18-19 0.13383406068505366 1102 20-21 0.13301666717781302 1102 22-23 0.2461637381530437 1102 24-25 0.26080673072200966 1102 26-27 0.2433970300117423 1102 28-29 0.20672573748343837 1102 30-31 0.25524741016736385 1102 32-33 0.24916310231903083 1102 34-35 0.27968465584940105 1102 36-37 0.2665030613722337 1102 38-39 0.24974892818647731 1102 40-41 0.2164554055131589 1102 42-43 0.22518077955871973 1102 44-45 0.3002638487025493 1102 46-47 0.18692827148428393 1102 48-49 0.2590911468869166 1102 50-51 0.15329198259750854 1102 52-53 0.2131421807574867 1102 54-55 0.15067520968585768 1102 56-57 0.14698612667787359 1102 58-59 0.13043153115901873 1102 60-61 0.09974792209717975 1102 62-63 0.03403231257026107 1102 64-65 0.07655320308226266 1102 66-67 0.04633318936594577 1102 68-69 0.09534704185978171 1102 70-71 0.09145332551266705 1102 72-73 0.12706838058002035 1102 74-75 0.08680600156516505 1102 76-77 0.06685433674165608 1102 78-79 0.04144523595876137 1102 80-81 0.03306671788467952 1102 82-83 0.04372594787018613 1102 84-85 0.07259438230390458 1102 86-87 0.06492391965154098 1102 88-89 0.13550460306357337 1102 90-91 0.02764939015175827 1102 92-93 0.1035705017952182 1102 94-95 0.12220513242521491 1102 96-97 0.059424328801988224 1102 98-99 -0.008626454131722028 1102 100 0.04843313167864949 1103 1 0.11600851202445028 1103 2 0.0090440037674 1103 3 0.07210983637123292 1103 4 0.10148178052674695 1103 5 0.10457187255713052 1103 6 0.1254619403856836 1103 7 0.12026383571352284 1103 8 0.16115374299643292 1103 9 0.17075824720496513 1103 10-11 0.21529569202009924 1103 12-13 0.1601434343386572 1103 14-15 0.2332368254413737 1103 16-17 0.2537457872083806 1103 18-19 0.254211585895348 1103 20-21 0.2442341718995209 1103 22-23 0.24436405551501394 1103 24-25 0.23370037925815268 1103 26-27 0.20171037787249446 1103 28-29 0.26518841532848114 1103 30-31 0.23832022828181465 1103 32-33 0.16664469954919525 1103 34-35 0.2007311846698414 1103 36-37 0.25942644389110114 1103 38-39 0.2656651018537062 1103 40-41 0.22866363228175146 1103 42-43 0.24152027853868674 1103 44-45 0.2530293027167403 1103 46-47 0.27098881932533203 1103 48-49 0.22865792931530393 1103 50-51 0.34231718585527204 1103 52-53 0.3153697431957738 1103 54-55 0.27830108075909266 1103 56-57 0.27994745885887085 1103 58-59 0.27195970033425 1103 60-61 0.3484517633563513 1103 62-63 0.3601759729876761 1103 64-65 0.38648948866227073 1103 66-67 0.3627463082261855 1103 68-69 0.38471310560323957 1103 70-71 0.34295443619155463 1103 72-73 0.3676749151034713 1103 74-75 0.38981385552585124 1103 76-77 0.3659316899815366 1103 78-79 0.36393650542424183 1103 80-81 0.33105242427468795 1103 82-83 0.29391395799478914 1103 84-85 0.3562150810634961 1103 86-87 0.3153860791210903 1103 88-89 0.308035410761228 1103 90-91 0.2703326169643532 1103 92-93 0.3002724367703422 1103 94-95 0.31707702151221895 1103 96-97 0.2860847805367399 1103 98-99 0.21838570524980838 1103 100 0.22016063339042802 1104 1 0.13901465344185482 1104 2 0.06783479029596862 1104 3 0.12883554607322623 1104 4 0.07261568932366913 1104 5 0.09932551797268019 1104 6 0.15469295151658002 1104 7 0.11835368169040805 1104 8 0.08181266730665726 1104 9 0.09910907819658377 1104 10-11 0.22098968311739497 1104 12-13 0.16553178397567336 1104 14-15 0.23813518178820203 1104 16-17 0.2291022357807364 1104 18-19 0.22630446053946685 1104 20-21 0.22339463744989985 1104 22-23 0.23207712166642125 1104 24-25 0.24137599487134764 1104 26-27 0.2531807991784447 1104 28-29 0.29553499676868 1104 30-31 0.2736250751583924 1104 32-33 0.2519898221505201 1104 34-35 0.22146876856358944 1104 36-37 0.20514061068809752 1104 38-39 0.15756860912099313 1104 40-41 0.18867589157333953 1104 42-43 0.21081035372564827 1104 44-45 0.14913611578861463 1104 46-47 0.1531021303262392 1104 48-49 0.09028474908016904 1104 50-51 0.09700302258975313 1104 52-53 0.17602183156357398 1104 54-55 0.1638779779643471 1104 56-57 0.1872180680060893 1104 58-59 0.1986173112690679 1104 60-61 0.2455958445064681 1104 62-63 0.23147411677285135 1104 64-65 0.2086226600518728 1104 66-67 0.1980975268401295 1104 68-69 0.19023800592292162 1104 70-71 0.1849391675718124 1104 72-73 0.14713231953060557 1104 74-75 0.08419825074254561 1104 76-77 0.20456245828093245 1104 78-79 0.159233768020286 1104 80-81 0.18530842905732925 1104 82-83 0.13253116792128594 1104 84-85 0.11542153156976553 1104 86-87 0.18667791668631395 1104 88-89 0.21819006449930356 1104 90-91 0.1555706599509108 1104 92-93 0.09580082232757547 1104 94-95 0.11501827192632419 1104 96-97 0.1952904532758808 1104 98-99 0.18222310735625769 1104 100 0.08846321759528308 1105 1 0.0666710701703579 1105 2 0.07064357788313913 1105 3 0.04749234737753483 1105 4 0.10958732640688851 1105 5 0.08010906559116648 1105 6 0.09815007733305237 1105 7 0.087041146746067 1105 8 0.0941955266608474 1105 9 0.11341481656106112 1105 10-11 0.08306825213430358 1105 12-13 0.15413549094937906 1105 14-15 0.1573695990956807 1105 16-17 0.1665430673792656 1105 18-19 0.16773353579414874 1105 20-21 0.11157907254175825 1105 22-23 0.1568477152793264 1105 24-25 0.14243408989105433 1105 26-27 0.16990915445185095 1105 28-29 0.17088293555673317 1105 30-31 0.16752262753502123 1105 32-33 0.18066018892990598 1105 34-35 0.11754723887872842 1105 36-37 0.12689041855065142 1105 38-39 0.14210162034952845 1105 40-41 0.14171551491774892 1105 42-43 0.13901369261396468 1105 44-45 0.10930483563267046 1105 46-47 0.1967045403345935 1105 48-49 0.15914984723126224 1105 50-51 0.19647710464824542 1105 52-53 0.13385225229190922 1105 54-55 0.0532945301309411 1105 56-57 0.03992839455848696 1105 58-59 0.02737741240227365 1105 60-61 0.017234251772848097 1105 62-63 0.0398766767597607 1105 64-65 -0.011755223288645311 1105 66-67 0.04017920737017988 1105 68-69 0.012028197253904693 1105 70-71 -0.0030615257479453817 1105 72-73 0.001111625861788923 1105 74-75 -0.0415119957651342 1105 76-77 -0.04533387476773498 1105 78-79 -0.04229042203839839 1105 80-81 0.07742679173939138 1105 82-83 0.04399477675895014 1105 84-85 -0.049728799051081296 1105 86-87 -0.04003704162723665 1105 88-89 -0.028534721862520485 1105 90-91 0.023534171591400366 1105 92-93 0.05719533992568415 1105 94-95 0.005302677467831529 1105 96-97 -0.04902080396200148 1105 98-99 -0.019945352492882762 1105 100 -0.004786484042504924 1106 1 0.044644023583231274 1106 2 0.07188625736086607 1106 3 0.04242886647350019 1106 4 0.04769861442822787 1106 5 0.05130629981800894 1106 6 0.051734979308704965 1106 7 0.08478180783891531 1106 8 0.053154076824085905 1106 9 0.06021394763315868 1106 10-11 0.11152825433994451 1106 12-13 0.04220918554718622 1106 14-15 0.11673538741455758 1106 16-17 0.06278593640366381 1106 18-19 0.06507735507920387 1106 20-21 0.10453813160908965 1106 22-23 0.07444818650385088 1106 24-25 0.06090805536828725 1106 26-27 0.10207887224557766 1106 28-29 0.07556307267364559 1106 30-31 0.08880676603965298 1106 32-33 0.04588575521495386 1106 34-35 0.0043053072487921895 1106 36-37 -0.06095622210791163 1106 38-39 -0.06123241947595659 1106 40-41 -0.03747645335788263 1106 42-43 -0.09388503317288155 1106 44-45 -0.08277242933520768 1106 46-47 -0.022051275301976148 1106 48-49 -0.061462377035766735 1106 50-51 -0.08738641370562306 1106 52-53 -0.08452088009897807 1106 54-55 -0.0937530506791262 1106 56-57 -0.07808149748032633 1106 58-59 -0.07532658576889162 1106 60-61 -0.06111946069978558 1106 62-63 -0.10225116270684964 1106 64-65 -0.11796181422855057 1106 66-67 -0.11039781865400045 1106 68-69 -0.10800567990607135 1106 70-71 -0.12407286063901068 1106 72-73 -0.1266929469229936 1106 74-75 -0.09408578111558086 1106 76-77 -0.08671894974779804 1106 78-79 -0.06580641518254993 1106 80-81 -0.13181205774675675 1106 82-83 -0.16625943979295243 1106 84-85 -0.07585981830672495 1106 86-87 -0.12005919561264733 1106 88-89 -0.10081920729499672 1106 90-91 -0.13347566645532538 1106 92-93 -0.07099407004423597 1106 94-95 -0.010246611559828978 1106 96-97 -0.06558459847962439 1106 98-99 -0.08451514472675825 1106 100 -0.028973501129271995 1107 1 -0.06457480379656744 1107 2 0.032926134199136925 1107 3 0.05114331213808043 1107 4 0.018940782246843924 1107 5 0.04762921177460555 1107 6 -0.001024855327301566 1107 7 0.053903634594291816 1107 8 -0.0415458779591944 1107 9 0.011719556659279817 1107 10-11 0.0723326274424565 1107 12-13 0.07150396624610522 1107 14-15 0.11345286905871887 1107 16-17 0.05669379119340334 1107 18-19 0.06728005516317381 1107 20-21 0.041570353903082946 1107 22-23 0.15487446172293318 1107 24-25 0.12590224179035658 1107 26-27 0.08607080859766114 1107 28-29 0.09950263299489848 1107 30-31 0.09751098411341275 1107 32-33 0.015103129200269905 1107 34-35 0.07640793843109606 1107 36-37 0.09406794730405466 1107 38-39 0.05616982628766465 1107 40-41 0.07725719864565406 1107 42-43 0.09436534901603011 1107 44-45 0.07030257658603034 1107 46-47 0.05226455270224761 1107 48-49 0.005315178247052188 1107 50-51 0.011558260411035803 1107 52-53 0.03884889575529371 1107 54-55 0.17123709853604652 1107 56-57 0.14621737425491688 1107 58-59 0.2215252002914383 1107 60-61 0.1954052039428973 1107 62-63 0.18078826196425268 1107 64-65 0.1726048481317619 1107 66-67 0.1349061022813416 1107 68-69 0.15403434765460133 1107 70-71 0.12124801656531048 1107 72-73 0.10662289184711682 1107 74-75 0.12630076798761536 1107 76-77 0.1743642597778603 1107 78-79 0.13384557758277538 1107 80-81 0.1194550869251998 1107 82-83 0.12050417366870647 1107 84-85 0.12117741012382766 1107 86-87 0.05208596081565986 1107 88-89 0.08559532234348666 1107 90-91 0.10454244176334981 1107 92-93 0.13122020336618334 1107 94-95 0.0741382795970722 1107 96-97 0.06803026274811685 1107 98-99 0.14812298157860937 1107 100 0.09076951719426063 1108 1 -0.005106292505971055 1108 2 0.05821500076017827 1108 3 0.037887009887690226 1108 4 0.042415940366666405 1108 5 0.02782140303840208 1108 6 0.04652960101101655 1108 7 0.032909444129138876 1108 8 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-0.05352759916576133 2214 64-65 -0.052544913610915955 2214 66-67 -0.06835121562199475 2214 68-69 -0.13098153616659403 2214 70-71 -0.1562967985016357 2214 72-73 -0.12216880318351997 2214 74-75 -0.15276208656196388 2214 76-77 -0.1360952364780985 2214 78-79 -0.16198038111929236 2214 80-81 -0.13020341091913679 2214 82-83 -0.108625619841618 2214 84-85 -0.07785027310740134 2214 86-87 -0.031102149009619495 2214 88-89 -0.018339327077889322 2214 90-91 -0.021735071284076923 2214 92-93 -0.04265091077041916 2214 94-95 -0.0587366920389627 2214 96-97 -0.06616760428160973 2214 98-99 -0.09749989112390978 2214 100 -0.09190350300087857 2215 1 -0.20152871655719906 2215 2 -0.1522797385870689 2215 3 -0.19581991004301358 2215 4 -0.16284415892435078 2215 5 -0.20395282889522548 2215 6 -0.19798863831685765 2215 7 -0.2438789287490266 2215 8 -0.2142670327759788 2215 9 -0.2598093193684008 2215 10-11 -0.2789118065335643 2215 12-13 -0.2655576217391342 2215 14-15 -0.3830370307808906 2215 16-17 -0.3293140376927184 2215 18-19 -0.3266149839251753 2215 20-21 -0.3154380694400061 2215 22-23 -0.37264216624794955 2215 24-25 -0.4312873226857903 2215 26-27 -0.4514702706221385 2215 28-29 -0.4594181472018306 2215 30-31 -0.42092559405767105 2215 32-33 -0.34302298540654874 2215 34-35 -0.3847745822907811 2215 36-37 -0.41822160932077423 2215 38-39 -0.4021621050245088 2215 40-41 -0.3993312913214808 2215 42-43 -0.5107234269634162 2215 44-45 -0.5034880399667898 2215 46-47 -0.4305444247196064 2215 48-49 -0.3244659942912733 2215 50-51 -0.29315309147383317 2215 52-53 -0.24539126486024543 2215 54-55 -0.3175893536774623 2215 56-57 -0.2449291425637199 2215 58-59 -0.2713026202947191 2215 60-61 -0.21004977996189922 2215 62-63 -0.15705397139936395 2215 64-65 -0.25828392111799303 2215 66-67 -0.3467313462644839 2215 68-69 -0.3257300006298891 2215 70-71 -0.22486558079665286 2215 72-73 -0.3310617543388261 2215 74-75 -0.3212148505280368 2215 76-77 -0.31303829975196606 2215 78-79 -0.2789195446173949 2215 80-81 -0.25778506247417 2215 82-83 -0.23907555647026157 2215 84-85 -0.2629662914363422 2215 86-87 -0.1830705608259393 2215 88-89 -0.22877707690727078 2215 90-91 -0.2588799479536661 2215 92-93 -0.25560304102293685 2215 94-95 -0.2700528663589381 2215 96-97 -0.22202604242141177 2215 98-99 -0.2217896444630938 2215 100 -0.2092731042488083 2216 1 -0.22292288639243907 2216 2 -0.15906257311396388 2216 3 -0.20937064989387721 2216 4 -0.17385289095292933 2216 5 -0.2367731263906947 2216 6 -0.23666522668296608 2216 7 -0.24850293832859904 2216 8 -0.2281832270733375 2216 9 -0.2987891571688337 2216 10-11 -0.31288336329175337 2216 12-13 -0.3536504938223075 2216 14-15 -0.47094422090273724 2216 16-17 -0.43305411404936933 2216 18-19 -0.38122927397159145 2216 20-21 -0.4107129767156579 2216 22-23 -0.38456018004190895 2216 24-25 -0.39943011429122777 2216 26-27 -0.45647855166438234 2216 28-29 -0.5058201315417179 2216 30-31 -0.49364543045849274 2216 32-33 -0.5184902616823308 2216 34-35 -0.5175886592850318 2216 36-37 -0.35514572698155433 2216 38-39 -0.4496542322026542 2216 40-41 -0.4630211371308093 2216 42-43 -0.46324545142553575 2216 44-45 -0.522213303754917 2216 46-47 -0.5095734044796458 2216 48-49 -0.5280981449518194 2216 50-51 -0.5785557522932336 2216 52-53 -0.5558515041940311 2216 54-55 -0.6051119613115787 2216 56-57 -0.6414408409938233 2216 58-59 -0.6142083719256561 2216 60-61 -0.6215300259827217 2216 62-63 -0.6550519030827076 2216 64-65 -0.6915609589153036 2216 66-67 -0.6184762746120853 2216 68-69 -0.5795539759967028 2216 70-71 -0.5396269856968487 2216 72-73 -0.5011706908663811 2216 74-75 -0.6054441693054002 2216 76-77 -0.5598556580606129 2216 78-79 -0.5772871295855566 2216 80-81 -0.6440094515043171 2216 82-83 -0.5438096689213694 2216 84-85 -0.6768521607902613 2216 86-87 -0.7600875194673833 2216 88-89 -0.8458837662787673 2216 90-91 -0.8346397432991708 2216 92-93 -0.8194025231662039 2216 94-95 -0.7492337818612071 2216 96-97 -0.8650538713887315 2216 98-99 -0.9610801962993065 2216 100 -0.9754429083807779 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14.0 3 5.0 4 4.0 5 25.0 6 59.0 7 128.0 8 312.0 9 588.0 10 932.0 11 1485.0 12 2387.0 13 3777.0 14 4301.0 15 5619.0 16 6989.0 17 10140.0 18 11792.0 19 6266.0 20 4255.0 21 4173.0 22 4235.0 23 4693.0 24 5248.0 25 6552.0 26 8174.0 27 10836.0 28 14212.0 29 18226.0 30 23139.0 31 29281.0 32 37572.0 33 49197.0 34 68574.0 35 105116.0 36 194843.0 37 435182.0 38 766905.0 39 355201.0 40 1285.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.272962895309135 19.622437119049632 13.290720510226986 23.813879475414247 2 15.965937366679613 20.102790730075533 39.745673077579774 24.185598825665082 3 18.463840920033068 25.243679768901643 30.0324300754885 26.260049235576794 4 12.41404213184597 16.723790231007513 38.450123997347454 32.41204363979906 5 12.76747270315935 37.59824137492506 35.66429882092182 13.96998710099377 6 31.19744163537672 37.97278399287102 17.436148754677593 13.393625617074658 7 27.28609697318735 32.423757404431626 22.476588779146507 17.813556843234522 8 25.45707571464777 35.951581358886756 20.18404751934175 18.407295407123723 9 25.17606673322063 16.186466774642756 21.08254357271263 37.55492291942398 10-11 24.12629115785668 26.41824112756199 28.784883176954594 20.67058453762673 12-13 25.13014812950954 23.672289235425726 29.02818793653331 22.16937469853142 14-15 22.11227755109827 25.517398153756154 26.502994145241875 25.8673301499037 16-17 21.3781335203389 29.056095765259567 27.00010946002597 22.565661254375563 18-19 21.208607410384893 27.60398463465288 29.68105436348834 21.50635359147389 20-21 22.33256514673515 26.973568870184337 29.3589971849307 21.334868798149813 22-23 21.84431304714338 26.867114543882632 29.328963112694417 21.959609296279567 24-25 21.76219365135743 26.976371429518746 29.43079412762598 21.83064079149784 26-27 21.57023671049757 26.86269228725373 29.50165302656697 22.06541797568173 28-29 21.579470167092623 26.99295957284169 29.508114817871355 21.919455442194327 30-31 21.92234296875692 27.42233967587606 29.033898278141244 21.621419077225777 32-33 21.567709274831245 27.066586971470514 29.514443694526378 21.851260059171867 34-35 21.576952040266665 26.876985377808825 29.614637996985994 21.931424584938515 36-37 22.035229697482244 27.17311722370036 28.98018005906286 21.811473019754537 38-39 21.937351763755824 27.353566889916163 29.040178551152234 21.668902795175775 40-41 21.721459459631316 27.161458841932145 29.215081488600497 21.902000209836043 42-43 21.664161430044626 27.112738827322165 29.028716847248848 22.19438289538436 44-45 21.882417489583155 27.238179933706437 28.92288399716222 21.956518579548188 46-47 22.024987714161913 27.506288259825716 28.487406675320496 21.981317350691867 48-49 22.046448189190098 27.454942086239768 28.321740891901886 22.176868832668248 50-51 22.071996373747456 27.436184041400324 28.226429131379895 22.265390453472328 52-53 22.13914835751289 27.4069569182667 28.166771281751284 22.287123442469124 54-55 22.184976123234453 27.87038963138852 27.943332536986958 22.00130170839007 56-57 21.879578802410112 27.60648256228534 28.115356979673184 22.398581655631364 58-59 21.773048550180267 27.201640352415062 28.469057401433968 22.5562536959707 60-61 22.027394920884653 27.588042450409272 28.127869912731946 22.256692715974133 62-63 21.904650087522405 27.56742222678431 28.09748460523172 22.430443080461565 64-65 21.90033528301938 27.28589258771089 28.302165305156603 22.511606824113127 66-67 21.784221622893355 27.408228650120225 28.15793728726878 22.64961243971764 68-69 22.17721441947353 27.458388157969438 28.149871214147865 22.214526208409165 70-71 22.149314592837737 27.314048263619295 28.135791986312526 22.40084515723045 72-73 21.992805155797832 27.526603686988622 28.19851156972824 22.282079587485303 74-75 22.015239917191938 27.566518509636566 27.955124134880126 22.463117438291373 76-77 22.03579743491685 27.614090243909082 27.863735748654918 22.486376572519145 78-79 22.215497687718976 27.63745831671755 27.903386531087936 22.243657464475532 80-81 22.142532072623155 27.37034920848318 28.06476021950092 22.422358499392747 82-83 21.963515334264876 27.46133192715966 28.197960730238343 22.377192008337122 84-85 21.941621149264076 27.447447952102944 27.949668486757183 22.661262411875796 86-87 22.101364278941958 27.563907640075623 27.833584085115564 22.501143995866855 88-89 21.8225280790813 27.430259617242896 27.956039094629176 22.791173209046622 90-91 21.737106292931614 27.395546455452628 27.997835293839785 22.869511957775966 92-93 21.92181662558872 27.536638024515963 27.89215738595732 22.649387963938004 94-95 21.778935463798593 27.308137765645796 27.97866203995985 22.934264730595764 96-97 21.71935733822406 27.354025356562467 27.997341167215783 22.929276137997693 98-99 21.590748317734533 27.495772052603456 27.89925233402908 23.01422729563293 100 21.814697768383112 27.2666122244316 27.80019457497359 23.1184954322117 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 29.0 1 27.0 2 92.0 3 162.0 4 111.0 5 80.0 6 200.5 7 478.0 8 813.5 9 958.0 10 784.0 11 628.5 12 694.0 13 825.0 14 997.0 15 1179.0 16 1326.5 17 1501.0 18 1794.0 19 2162.5 20 2616.5 21 3212.0 22 4138.0 23 5670.0 24 7621.0 25 9863.5 26 12977.0 27 17238.0 28 22526.5 29 28118.0 30 33536.0 31 39345.5 32 45136.5 33 50897.0 34 56347.0 35 61324.0 36 65643.5 37 69378.0 38 72594.5 39 74500.5 40 75863.5 41 76641.0 42 77198.0 43 78116.0 44 77633.0 45 76857.0 46 75987.5 47 73783.5 48 72317.5 49 71342.5 50 69561.5 51 67139.0 52 63145.5 53 60248.5 54 59120.5 55 56506.0 56 52109.5 57 48064.5 58 43529.0 59 37980.5 60 33352.0 61 28907.5 62 24832.5 63 20771.0 64 16920.0 65 13957.0 66 11359.5 67 9155.0 68 7580.0 69 6135.0 70 4742.5 71 3589.0 72 2709.0 73 2138.0 74 1620.0 75 1086.5 76 739.5 77 544.5 78 363.0 79 239.0 80 153.0 81 76.5 82 42.5 83 25.5 84 12.5 85 7.0 86 2.5 87 1.5 88 2.0 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044328938894192815 2 0.2459438566721866 3 0.002815977675655691 4 0.002815977675655691 5 9.083798953728036E-5 6 1.3625698430592056E-4 7 0.0 8 3.6335195814912144E-4 9 0.0 10-11 7.267039162982429E-4 12-13 0.0 14-15 3.860614555334416E-4 16-17 2.270949738432009E-4 18-19 6.585754241452826E-4 20-21 0.0 22-23 1.1354748692160045E-4 24-25 0.0010446368796787242 26-27 1.3625698430592056E-4 28-29 4.087709529177616E-4 30-31 1.1354748692160045E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.3625698430592056E-4 42-43 2.270949738432009E-5 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.270949738432009E-4 70-71 6.812849215296028E-5 72-73 2.952234659961612E-4 74-75 1.8167597907456072E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.1354748692160045E-4 84-85 0.0 86-87 4.3148045030208174E-4 88-89 0.0017486312985926472 90-91 0.0017032123038240067 92-93 6.131564293766425E-4 94-95 5.450279372236822E-4 96-97 1.8167597907456072E-4 98-99 1.5896648169024064E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2201722.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.01159241700991 #Duplication Level Percentage of deduplicated Percentage of total 1 80.58130419077251 54.804628170539125 2 12.28748427530972 16.71382744725566 3 3.3995552792969868 6.93627504163923 4 1.3791766997017871 3.7520001428461924 5 0.6830034344086399 2.3226075600209186 6 0.41390459706716004 1.6890186453155047 7 0.2683562411560063 1.2775934707244185 8 0.17797742607727174 0.9683622529436732 9 0.12756212802620626 0.7808133113248299 >10 0.6403997989879248 7.59512278709757 >50 0.0296325889891786 1.3419301557732763 >100 0.01117569971324477 1.3166654856312487 >500 2.672231390865055E-4 0.11263504588554184 >1k 2.0041735431413144E-4 0.3885204830029325 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3718 0.1688678225498042 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3182 0.14452324135381306 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.270949738432009E-5 0.0 16-17 0.0 0.0 0.0 6.812849215296027E-5 0.0 18-19 0.0 0.0 0.0 9.083798953728036E-5 0.0 20-21 0.0 0.0 0.0 9.083798953728036E-5 0.0 22-23 0.0 0.0 0.0 1.3625698430592053E-4 0.0 24-25 0.0 0.0 0.0 1.3625698430592053E-4 0.0 26-27 0.0 0.0 0.0 2.0438547645888083E-4 0.0 28-29 0.0 0.0 0.0 6.812849215296027E-4 0.0 30-31 0.0 0.0 0.0 0.0029295251625772916 0.0 32-33 0.0 0.0 0.0 0.009061089456343718 0.0 34-35 0.0 0.0 0.0 0.016918575551318468 0.0 36-37 0.0 0.0 0.0 0.031202849406055805 0.0 38-39 0.0 0.0 0.0 0.056364972507882465 0.0 40-41 0.0 0.0 0.0 0.09088340853204901 0.0 42-43 0.0 0.0 0.0 0.12556081103790578 0.0 44-45 0.0 0.0 0.0 0.16364463815141056 0.0 46-47 0.0 0.0 0.0 0.21156167763232597 0.0 48-49 0.0 0.0 0.0 0.265269638946243 0.0 50-51 0.0 0.0 0.0 0.32919687408310405 0.0 52-53 0.0 0.0 0.0 0.3910802544553763 0.0 54-55 0.0 4.541899476864018E-5 0.0 0.45802785274435187 0.0 56-57 0.0 9.083798953728036E-5 0.0 0.536284780730719 0.0 58-59 0.0 9.083798953728036E-5 0.0 0.6299841669384236 0.0 60-61 0.0 1.3625698430592053E-4 0.0 0.726999139764239 0.0 62-63 0.0 1.3625698430592053E-4 0.0 0.8258308723808001 0.0 64-65 0.0 1.3625698430592053E-4 0.0 0.9368348955953567 0.0 66-67 0.0 1.3625698430592053E-4 0.0 1.0671874105813541 0.0 68-69 0.0 1.3625698430592053E-4 0.0 1.2180466017053924 0.0 70-71 0.0 1.3625698430592053E-4 0.0 1.3747875526519697 0.0 72-73 0.0 1.3625698430592053E-4 0.0 1.5452450400186764 0.0 74-75 0.0 1.3625698430592053E-4 0.0 1.7153164659298494 0.0 76-77 0.0 1.3625698430592053E-4 0.0 1.8983095958527008 0.0 78-79 0.0 1.3625698430592053E-4 0.0 2.1047389270761707 0.0 80-81 0.0 1.3625698430592053E-4 0.0 2.339146359077122 0.0 82-83 0.0 1.3625698430592053E-4 0.0 2.588224126388345 0.0 84-85 0.0 1.3625698430592053E-4 0.0 2.8353488769245163 0.0 86-87 0.0 1.3625698430592053E-4 0.0 3.0936921191685416 0.0 88 0.0 1.3625698430592053E-4 0.0 3.3159499700688824 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3120 0.0 51.970196 1 GTATCAA 5345 0.0 40.124924 1 TCAACGC 6470 0.0 32.618782 4 ATCAACG 6475 0.0 32.521 3 CAACGCA 6670 0.0 31.568085 5 TATCAAC 6805 0.0 30.949375 2 AACGCAG 6875 0.0 30.695147 6 ACGCAGA 8100 0.0 25.878319 7 CGCAGAG 8335 0.0 25.092878 8 GTACATG 6165 0.0 24.619164 1 TACATGG 6225 0.0 24.144812 2 ACATGGG 5785 0.0 23.96869 3 GCAGAGT 9275 0.0 22.447407 9 GAGTACT 6490 0.0 20.167952 12-13 AGAGTAC 8675 0.0 19.774237 10-11 GTGGTAT 1560 0.0 19.337748 1 CAGAGTA 9360 0.0 19.306202 10-11 CATGGGG 4280 0.0 19.218521 4 GAGTACA 4425 0.0 19.067348 1 AGTACTT 6760 0.0 18.91052 12-13 >>END_MODULE