FastQCFastQC Report
Wed 25 May 2016
SRR1033012_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033012_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3451414
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46150.036.879391
TCAACGC44600.035.6253664
GGTATCA26300.035.574941
CAACGCA45250.035.0097355
ATCAACG46050.034.809863
AACGCAG47350.033.6507156
TATCAAC54350.029.839882
GTACATG101250.028.8829921
TACATGG102150.027.7495842
ACGCAGA60000.026.1647197
CGCAGAG61950.025.2872668
ACATGGG107750.024.9985523
CATGGGG74250.021.779084
GCAGAGT75300.020.1188879
GAGTACA74000.020.0771561
AGTACAT75700.018.381182
CTATACG2854.820322E-818.1436794
GTATAGG12350.017.512081
AGAGTAC62150.016.88942710-11
TCTATAC14300.016.7653143
Produced by FastQC (version 0.11.5)