##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033012_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3451414 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.67649201167985 33.0 31.0 34.0 30.0 34.0 2 31.741095388730532 33.0 31.0 34.0 30.0 34.0 3 31.786224428596512 34.0 31.0 34.0 30.0 34.0 4 35.17049418006649 37.0 35.0 37.0 33.0 37.0 5 35.21793560552284 37.0 35.0 37.0 33.0 37.0 6 35.28248827871707 37.0 35.0 37.0 33.0 37.0 7 35.16052290452551 37.0 35.0 37.0 33.0 37.0 8 35.16625215056786 37.0 35.0 37.0 33.0 37.0 9 36.79198612510699 39.0 37.0 39.0 33.0 39.0 10-11 36.798270795679684 39.0 37.5 39.0 33.0 39.0 12-13 36.77489327562559 39.0 37.5 39.0 33.0 39.0 14-15 38.183098869043235 41.0 39.0 41.0 34.0 41.0 16-17 38.19394094710168 41.0 39.0 41.0 34.0 41.0 18-19 38.19386648486678 41.0 38.5 41.0 34.0 41.0 20-21 38.20726678978529 40.0 39.0 41.0 34.0 41.0 22-23 38.178640261643494 40.0 39.0 41.0 34.0 41.0 24-25 38.19334916066285 40.0 38.5 41.0 34.0 41.0 26-27 38.10127733155165 40.0 38.0 41.0 33.0 41.0 28-29 38.11669825758371 40.0 38.0 41.0 33.0 41.0 30-31 38.27115350404211 40.0 39.0 41.0 34.0 41.0 32-33 38.25653196052401 40.5 38.5 41.0 33.5 41.0 34-35 38.212970249294926 40.0 38.0 41.0 33.0 41.0 36-37 38.13858667780799 40.0 38.0 41.0 33.0 41.0 38-39 38.044531024096216 40.0 38.0 41.0 33.0 41.0 40-41 37.96634234548507 40.0 38.0 41.0 33.0 41.0 42-43 37.846918972919504 40.0 38.0 41.0 33.0 41.0 44-45 37.71214305209401 40.0 38.0 41.0 32.5 41.0 46-47 37.57311148416272 40.0 38.0 41.0 32.0 41.0 48-49 37.45195360510214 40.0 37.5 41.0 32.0 41.0 50-51 37.32328344267016 40.0 37.0 41.0 31.5 41.0 52-53 37.16325453857463 40.0 37.0 41.0 31.0 41.0 54-55 37.00175696106002 40.0 36.5 41.0 31.0 41.0 56-57 36.82186257574432 40.0 36.0 41.0 31.0 41.0 58-59 36.61968659801461 39.5 35.5 41.0 30.5 41.0 60-61 36.395142976183095 39.0 35.0 41.0 30.0 41.0 62-63 36.135876339378584 39.0 35.0 41.0 30.0 41.0 64-65 35.85927332971356 38.5 35.0 40.5 30.0 41.0 66-67 35.558308275970376 38.0 35.0 40.0 29.5 41.0 68-69 35.24081072279361 37.0 35.0 40.0 29.0 41.0 70-71 34.90620786147359 37.0 34.0 39.5 29.0 41.0 72-73 34.55591853078188 36.0 34.0 39.0 29.0 41.0 74-75 34.20146235716723 36.0 34.0 39.0 28.5 40.5 76-77 33.8373750584543 35.0 34.0 38.5 28.0 40.0 78-79 33.45785872109228 35.0 34.0 37.0 28.0 39.0 80-81 33.112034371999414 35.0 33.5 37.0 27.0 39.0 82-83 32.892956046420395 35.0 33.0 36.0 27.0 39.0 84-85 32.674858478293245 35.0 33.0 36.0 27.0 37.0 86-87 32.50644808185862 35.0 33.0 36.0 27.0 37.0 88-89 32.29223022795874 35.0 33.0 35.0 27.0 37.0 90-91 32.088504016035166 35.0 33.0 35.0 27.0 36.0 92-93 31.87342115434428 35.0 33.0 35.0 26.0 36.0 94-95 31.67458221470968 35.0 33.0 35.0 25.5 36.0 96-97 31.501603980281708 35.0 33.0 35.0 25.0 35.5 98-99 31.325138044870883 35.0 33.0 35.0 25.0 35.0 100 31.185440228265865 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1009450697630001 1101 2 0.19983655974724357 1101 3 0.17975541889822466 1101 4 0.05206334368379828 1101 5 0.11687760508689848 1101 6 0.21747598592843786 1101 7 0.19762220173194578 1101 8 0.18308300007271328 1101 9 0.3034806051518828 1101 10-11 0.27794954200557953 1101 12-13 0.2464128124953433 1101 14-15 0.36833818218437386 1101 16-17 0.37296734502521645 1101 18-19 0.39356276254302003 1101 20-21 0.3223703718433697 1101 22-23 0.3273620151128753 1101 24-25 0.3663998328419069 1101 26-27 0.2549809486742305 1101 28-29 0.23842863062758823 1101 30-31 0.2836908002441163 1101 32-33 0.24238189111626696 1101 34-35 0.3081707072214712 1101 36-37 0.3018917530066574 1101 38-39 0.34586302005335057 1101 40-41 0.29767836619598853 1101 42-43 0.3474861526774191 1101 44-45 0.36873171824545636 1101 46-47 0.30311597163948534 1101 48-49 0.3618201155817289 1101 50-51 0.3235062342309192 1101 52-53 0.2506612551007805 1101 54-55 0.25652785803184486 1101 56-57 0.31439260344048137 1101 58-59 0.39834008184343617 1101 60-61 0.3821301033678708 1101 62-63 0.35368174012644005 1101 64-65 0.317286807794531 1101 66-67 0.2953787762078619 1101 68-69 0.32595947322828067 1101 70-71 0.29784781617173195 1101 72-73 0.27558422887764067 1101 74-75 0.2408447295983578 1101 76-77 0.2813393945224334 1101 78-79 0.37296138316010286 1101 80-81 0.4035093170194983 1101 82-83 0.3470857474488369 1101 84-85 0.3017280461234648 1101 86-87 0.2746717066859645 1101 88-89 0.27187992263873184 1101 90-91 0.3477920365435665 1101 92-93 0.3532629298393104 1101 94-95 0.23941814669789352 1101 96-97 0.20946966010851398 1101 98-99 0.19588727958760188 1101 100 0.15788323366116686 1102 1 0.037162871268328956 1102 2 0.1180125732560775 1102 3 0.07025199433683582 1102 4 -0.09316575024579521 1102 5 -0.01475141143117753 1102 6 0.03605307571195482 1102 7 0.05238183109995731 1102 8 0.03755397393434379 1102 9 0.053961994007394765 1102 10-11 0.044682998568475796 1102 12-13 0.004349060851566833 1102 14-15 0.11407360005122769 1102 16-17 0.11746763644141112 1102 18-19 0.04694231538293536 1102 20-21 0.1558118164666169 1102 22-23 0.13709477785442914 1102 24-25 0.1304682911285866 1102 26-27 0.07414108140429221 1102 28-29 0.06506905392785 1102 30-31 0.15832973520684135 1102 32-33 0.15843975622781414 1102 34-35 0.07115192294919126 1102 36-37 0.1891789368331871 1102 38-39 0.17601452184808153 1102 40-41 0.044865601006321754 1102 42-43 0.1140037141995478 1102 44-45 0.08953226491753696 1102 46-47 0.014797830504207354 1102 48-49 0.051188094313779686 1102 50-51 0.11767108820277628 1102 52-53 0.08794024109221255 1102 54-55 0.047425088623469946 1102 56-57 0.09766137882242276 1102 58-59 0.016600708053225333 1102 60-61 -0.005933348062953314 1102 62-63 0.10516296539957892 1102 64-65 0.0994849047363644 1102 66-67 0.11268028846512124 1102 68-69 0.020700824044340038 1102 70-71 -0.04435225723445768 1102 72-73 -0.03323905143927419 1102 74-75 -0.07494977538154757 1102 76-77 -0.02493588915447731 1102 78-79 -0.06106752150805761 1102 80-81 0.021696083168201596 1102 82-83 -0.03321144840491996 1102 84-85 -0.07959568058242894 1102 86-87 -0.0933191209098112 1102 88-89 -0.05289053329939719 1102 90-91 -0.04869511157184547 1102 92-93 0.013005514073938684 1102 94-95 -0.0020458341109375056 1102 96-97 -0.02246941425483584 1102 98-99 -0.025719738470471043 1102 100 -0.09239391118634543 1103 1 -0.02853769353122715 1103 2 0.04151022131262749 1103 3 -2.1257164073418267E-4 1103 4 -0.021167406334356542 1103 5 -0.036386305390827545 1103 6 -0.04959612659771295 1103 7 -0.0030556714915590533 1103 8 -0.015583459798193644 1103 9 0.05659311224106034 1103 10-11 0.10116361900098525 1103 12-13 0.12401769517919803 1103 14-15 0.14547170400170728 1103 16-17 0.07683725074253545 1103 18-19 0.14218182714690641 1103 20-21 0.12988929486711953 1103 22-23 0.1392430991377296 1103 24-25 0.06123604570568375 1103 26-27 0.028443000028779863 1103 28-29 0.07268223710868682 1103 30-31 0.09812798779064735 1103 32-33 0.030554713865186045 1103 34-35 0.023582435805046487 1103 36-37 0.11824557251391354 1103 38-39 0.18350096446140185 1103 40-41 0.21103612922167514 1103 42-43 0.14406444158142762 1103 44-45 0.16922111660280592 1103 46-47 0.20749514620898424 1103 48-49 0.11098496741443853 1103 50-51 0.08112253730684671 1103 52-53 0.1349603049967456 1103 54-55 0.14856127063231384 1103 56-57 0.24149807938226786 1103 58-59 0.15360515730720437 1103 60-61 0.08732788564329041 1103 62-63 0.09420338381267612 1103 64-65 0.06418433423104375 1103 66-67 0.0677785994532556 1103 68-69 0.033310646673108124 1103 70-71 0.10999000041862672 1103 72-73 0.15099661478286208 1103 74-75 0.18221675915479807 1103 76-77 0.13613693054450238 1103 78-79 0.16940818488122034 1103 80-81 0.17960924855839977 1103 82-83 0.18880843387209012 1103 84-85 0.12728455139755113 1103 86-87 0.1263388284038811 1103 88-89 0.12012458360315748 1103 90-91 0.11564303864137315 1103 92-93 0.14658617387975426 1103 94-95 0.10226800991053864 1103 96-97 0.11930074170383875 1103 98-99 0.10771896392584424 1103 100 0.07544572961290186 1104 1 -0.07188021071577211 1104 2 -0.039236260292131675 1104 3 -0.010676975066552075 1104 4 -0.04405150801105151 1104 5 -0.08748667180115177 1104 6 -0.006787458170677496 1104 7 0.05458021428289328 1104 8 0.06970500211167519 1104 9 0.0696524782009007 1104 10-11 0.09233732944764483 1104 12-13 0.15532516366686622 1104 14-15 0.1449154223056368 1104 16-17 0.12784591163956804 1104 18-19 0.16294428643706027 1104 20-21 0.1310679049052652 1104 22-23 0.17908019907535078 1104 24-25 0.02092201767398194 1104 26-27 -0.025772318913482195 1104 28-29 0.024969308091463915 1104 30-31 0.06891626936685213 1104 32-33 0.07109060938958578 1104 34-35 0.025073557688408243 1104 36-37 0.09558299875845933 1104 38-39 0.08979917432139217 1104 40-41 0.15330872894286784 1104 42-43 0.13247832755766353 1104 44-45 0.12982590523083104 1104 46-47 0.08933525585320723 1104 48-49 0.05908486780540301 1104 50-51 0.04241209297633475 1104 52-53 0.029857255038187702 1104 54-55 -0.006601147026387366 1104 56-57 -0.004158770005105339 1104 58-59 0.05957130874070771 1104 60-61 0.05023993489880496 1104 62-63 0.04462383056389996 1104 64-65 0.007031043149169136 1104 66-67 0.00793112730585932 1104 68-69 0.03266870808010225 1104 70-71 0.02399794647532616 1104 72-73 0.050831442987508524 1104 74-75 0.07054894424143754 1104 76-77 -0.017310502755918833 1104 78-79 0.07862327100482247 1104 80-81 0.14678024531986722 1104 82-83 0.1336402562327592 1104 84-85 0.09600834470602138 1104 86-87 0.028258625279036664 1104 88-89 0.06422318632183988 1104 90-91 0.1821027308529679 1104 92-93 0.1466132122560424 1104 94-95 0.18664890346477847 1104 96-97 0.1846822309088445 1104 98-99 0.1743142088135201 1104 100 0.20244765714776136 1105 1 -0.06507282635993761 1105 2 -0.012802998094379348 1105 3 -0.04042559530094891 1105 4 -0.10683331334353596 1105 5 -0.09174583052602969 1105 6 0.028947596403078535 1105 7 -0.026191606735594064 1105 8 -0.004738077191127843 1105 9 0.04796124388212064 1105 10-11 0.05095122447850997 1105 12-13 0.08565083991211253 1105 14-15 0.01934774613678769 1105 16-17 0.032473477470638556 1105 18-19 0.012638220411425038 1105 20-21 0.06457028940379672 1105 22-23 0.06573266277673895 1105 24-25 0.04971480171856513 1105 26-27 -0.00239490679668819 1105 28-29 0.061533202174381074 1105 30-31 0.0918536007515911 1105 32-33 0.021999589640856243 1105 34-35 0.021384455062239738 1105 36-37 0.05090015254025104 1105 38-39 0.06545781513327853 1105 40-41 0.17395726214731866 1105 42-43 0.060657397627188914 1105 44-45 0.06637256721263896 1105 46-47 0.04889735922255056 1105 48-49 0.035620852405436665 1105 50-51 0.024202479701955326 1105 52-53 0.11516364248014099 1105 54-55 0.09188769713033196 1105 56-57 0.06767214254875853 1105 58-59 0.08180180598524345 1105 60-61 0.0376008669924488 1105 62-63 -0.02836311405656744 1105 64-65 0.035697937457214834 1105 66-67 0.07349363666205022 1105 68-69 0.08332410152045355 1105 70-71 -0.012328659039461343 1105 72-73 0.02933450755225664 1105 74-75 0.02939926241138835 1105 76-77 0.0832350234609649 1105 78-79 -0.01576925597110801 1105 80-81 0.027557613134739256 1105 82-83 0.00724305626201982 1105 84-85 -0.0037530625443977783 1105 86-87 -0.03599555662505338 1105 88-89 -0.02440612662933006 1105 90-91 -0.08493711418451255 1105 92-93 -0.0840717700020619 1105 94-95 -0.06696193821165508 1105 96-97 -0.0012612773448736903 1105 98-99 -0.03773667728719943 1105 100 -0.0803398567962148 1106 1 -0.009372913725716359 1106 2 -0.012460580598677495 1106 3 -0.08568388800449966 1106 4 -0.09088483097169586 1106 5 -0.06306871754235033 1106 6 0.002899911596266236 1106 7 -0.04120442977761485 1106 8 0.05419296020706099 1106 9 0.05902569728234397 1106 10-11 -0.023120866381901806 1106 12-13 0.014554822100770082 1106 14-15 0.057175015533402984 1106 16-17 0.055759980866632475 1106 18-19 0.06633012921282955 1106 20-21 0.02934215198587964 1106 22-23 0.04493115219420929 1106 24-25 0.019630676926404078 1106 26-27 0.03666393828404324 1106 28-29 0.018963520918568122 1106 30-31 0.03673565694751346 1106 32-33 -0.020393950927868332 1106 34-35 0.030623386381471107 1106 36-37 0.05734267431124351 1106 38-39 0.09803679661737164 1106 40-41 0.1298361337196141 1106 42-43 0.1094843006511752 1106 44-45 0.07392759356328327 1106 46-47 0.016080940092265905 1106 48-49 0.15756516376580265 1106 50-51 0.11178667495038752 1106 52-53 0.09016434704288656 1106 54-55 0.1453097583991081 1106 56-57 0.11318407784171569 1106 58-59 0.14403019704574405 1106 60-61 0.1978275952848776 1106 62-63 0.14811153577574743 1106 64-65 0.1954258353238032 1106 66-67 0.18440238169488765 1106 68-69 0.17949480298312182 1106 70-71 0.04671933577495935 1106 72-73 0.1029204501163079 1106 74-75 0.12420945320698706 1106 76-77 0.06224469006187405 1106 78-79 0.09356831413288802 1106 80-81 0.13372324174562067 1106 82-83 0.12775640068047522 1106 84-85 0.19587140551072935 1106 86-87 0.1891073095362117 1106 88-89 0.2136289887780407 1106 90-91 0.1814714476620054 1106 92-93 0.2330899694224513 1106 94-95 0.20237055904054913 1106 96-97 0.12772232043434428 1106 98-99 0.07109994870270953 1106 100 0.08345689094214137 1107 1 -0.05788255746534432 1107 2 -0.0861564594904678 1107 3 0.01091071097511076 1107 4 -0.026797209643866893 1107 5 -0.07127705973706355 1107 6 -0.018059171117499773 1107 7 -0.06204179184155123 1107 8 -0.010643073197968533 1107 9 0.026657432205389853 1107 10-11 0.018111921528912944 1107 12-13 0.031689113851761874 1107 14-15 0.11817874864104994 1107 16-17 0.030324914503118805 1107 18-19 0.06413188570282813 1107 20-21 0.049018801134415924 1107 22-23 0.10587435385291144 1107 24-25 0.0739189820239119 1107 26-27 -0.0012540539354759517 1107 28-29 -0.005097044879640578 1107 30-31 0.0771850257614517 1107 32-33 0.06083896122497379 1107 34-35 0.04706522978242589 1107 36-37 0.13548075398253445 1107 38-39 0.13464361516627577 1107 40-41 0.08388225820866779 1107 42-43 0.06353523038353615 1107 44-45 0.10178855869001069 1107 46-47 0.12671340877413684 1107 48-49 0.1197334284042526 1107 50-51 0.14887968180489253 1107 52-53 0.1342325939220359 1107 54-55 0.1530705552144198 1107 56-57 0.15154884806338487 1107 58-59 0.09862086705379625 1107 60-61 0.11141238352894334 1107 62-63 0.1083335124372411 1107 64-65 0.17159118904451987 1107 66-67 0.22577890667447065 1107 68-69 0.20757381287643994 1107 70-71 0.23769629472466391 1107 72-73 0.1948628792819065 1107 74-75 0.17280244873354178 1107 76-77 0.20379305273460346 1107 78-79 0.20045528750197406 1107 80-81 0.14482688006390987 1107 82-83 0.12910163128464802 1107 84-85 0.09789580153001509 1107 86-87 0.11114567182158908 1107 88-89 0.12488487839850393 1107 90-91 0.052206364861994814 1107 92-93 0.10892639236279322 1107 94-95 0.11042577293051536 1107 96-97 0.07934300293236873 1107 98-99 0.0600861483362678 1107 100 0.09260987454145919 1108 1 0.041853256362450963 1108 2 0.10657121544216963 1108 3 0.13993989629285153 1108 4 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-0.04917169928715026 2214 10-11 -0.010127197574277602 2214 12-13 0.0061878904780243715 2214 14-15 -0.06904811708508163 2214 16-17 -0.07065394147752357 2214 18-19 -0.08461920429464698 2214 20-21 -0.04157279102208378 2214 22-23 -0.06693077594678698 2214 24-25 -0.03008668616304533 2214 26-27 -0.018163525264348834 2214 28-29 0.023408164898093275 2214 30-31 -0.008692045456932362 2214 32-33 0.0015832861656477348 2214 34-35 -0.021563600107846526 2214 36-37 -0.02306258981495546 2214 38-39 -0.05898437203170204 2214 40-41 -0.043454843059727466 2214 42-43 -0.029060953591752536 2214 44-45 -0.0076073234367441955 2214 46-47 -0.016453009302153987 2214 48-49 0.04079309234256101 2214 50-51 0.02564198996849143 2214 52-53 0.019199077240415363 2214 54-55 0.07996292735460031 2214 56-57 0.009891518735201998 2214 58-59 0.011170693256012498 2214 60-61 0.015623234592140989 2214 62-63 0.016884528967345602 2214 64-65 0.03971346158817113 2214 66-67 0.06330576931920007 2214 68-69 -0.004685708522970344 2214 70-71 0.11907130812692657 2214 72-73 0.12969829201042415 2214 74-75 0.09707910398361719 2214 76-77 0.11370021128483643 2214 78-79 0.15321757436354488 2214 80-81 0.1262274220404933 2214 82-83 0.08852757482373619 2214 84-85 0.10517569330338006 2214 86-87 0.14370988797599438 2214 88-89 0.15892793872815503 2214 90-91 0.11763234835779457 2214 92-93 0.15152009366652663 2214 94-95 0.13601873047941737 2214 96-97 0.06835721979017606 2214 98-99 0.08881670711713952 2214 100 0.07765628049036621 2215 1 -0.05963199533871588 2215 2 -0.16688193442620758 2215 3 -0.13145252735983703 2215 4 -0.1218997277823135 2215 5 -0.11205195595551487 2215 6 -0.07272517056087935 2215 7 -0.09792266820606699 2215 8 -0.10847590139291441 2215 9 -0.14235912833051145 2215 10-11 -0.1375967267009912 2215 12-13 -0.17149053781632517 2215 14-15 -0.3218272221980527 2215 16-17 -0.2817902687382414 2215 18-19 -0.22374146596565225 2215 20-21 -0.20827252410860808 2215 22-23 -0.2328018913345602 2215 24-25 -0.1435671238604712 2215 26-27 -0.13171702326793167 2215 28-29 -0.1686889092516708 2215 30-31 -0.20356720105129966 2215 32-33 -0.22282045276054419 2215 34-35 -0.21213555002354667 2215 36-37 -0.1489238908626902 2215 38-39 -0.17765294130514775 2215 40-41 -0.1855615680201268 2215 42-43 -0.15340963332352686 2215 44-45 -0.0998671801721045 2215 46-47 -0.1002325654730214 2215 48-49 -0.07612690971249236 2215 50-51 -0.09008008831780501 2215 52-53 -0.10652139536731653 2215 54-55 -0.10580627959341626 2215 56-57 -0.1976438522261219 2215 58-59 -0.12222698310691982 2215 60-61 -0.25625664674473114 2215 62-63 -0.30235496397816775 2215 64-65 -0.38798002557611966 2215 66-67 -0.2881961839559395 2215 68-69 -0.2693702055612235 2215 70-71 -0.24475496852594603 2215 72-73 -0.28872733951248364 2215 74-75 -0.24970523175282722 2215 76-77 -0.12152411437201494 2215 78-79 -0.2864934833279662 2215 80-81 -0.33630621254749116 2215 82-83 -0.23303232323176815 2215 84-85 -0.276956130202791 2215 86-87 -0.27747704713128485 2215 88-89 -0.28917428955967495 2215 90-91 -0.2578038727890899 2215 92-93 -0.26108749613268856 2215 94-95 -0.3501139495603489 2215 96-97 -0.2491962591564416 2215 98-99 -0.17946620958316473 2215 100 -0.15765317059547357 2216 1 -0.09825970481299606 2216 2 -0.09001058956678065 2216 3 -0.047093673619663434 2216 4 -0.11393096477577558 2216 5 -0.08617070531425952 2216 6 -0.12466693222974357 2216 7 -0.05706601263186428 2216 8 0.01346220150563937 2216 9 0.0016159997748417254 2216 10-11 -0.049538062481246925 2216 12-13 -0.15304704452309892 2216 14-15 -0.15310738527468004 2216 16-17 -0.17002103067232355 2216 18-19 -0.14341693884720286 2216 20-21 -0.06577038982592853 2216 22-23 -0.019046657260375355 2216 24-25 -0.07548902363339494 2216 26-27 -0.1390576005792994 2216 28-29 -0.0390015695648529 2216 30-31 -0.20691681494456304 2216 32-33 -0.2116377794577602 2216 34-35 -0.3623423963764836 2216 36-37 -0.34258086082012085 2216 38-39 -0.273981781513676 2216 40-41 -0.17294035915467276 2216 42-43 -0.2411035820271792 2216 44-45 -0.18469838309447084 2216 46-47 -0.1451448420992918 2216 48-49 -0.280133747160491 2216 50-51 -0.09719165371009808 2216 52-53 -0.16717974171929484 2216 54-55 -0.20427637156599587 2216 56-57 -0.225292540586004 2216 58-59 -0.2364361757189144 2216 60-61 -0.16517339907309037 2216 62-63 -0.24469777720471342 2216 64-65 -0.15807683779989645 2216 66-67 -0.19951049555540123 2216 68-69 -0.1321041781146377 2216 70-71 -0.11453217070882005 2216 72-73 -0.1281634629969446 2216 74-75 -0.1935953757225377 2216 76-77 -0.025204372692805066 2216 78-79 -0.06754434410691346 2216 80-81 -0.0351568940660556 2216 82-83 -0.14947665668706378 2216 84-85 -0.020989334027277096 2216 86-87 -8.486523635227172E-4 2216 88-89 -0.14264537849877001 2216 90-91 -0.12525281939233324 2216 92-93 -0.25755126559531405 2216 94-95 -0.24273639737447894 2216 96-97 -0.18737484973617313 2216 98-99 -0.12141797963428758 2216 100 -0.12144063669377303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36239.0 3 7107.0 4 3724.0 5 3419.0 6 2884.0 7 3148.0 8 3779.0 9 4281.0 10 4939.0 11 5254.0 12 6034.0 13 6689.0 14 7514.0 15 7615.0 16 7508.0 17 7485.0 18 7431.0 19 7168.0 20 7139.0 21 7451.0 22 8639.0 23 12753.0 24 20343.0 25 26461.0 26 26202.0 27 22910.0 28 24908.0 29 31321.0 30 37950.0 31 47293.0 32 62639.0 33 85265.0 34 124928.0 35 194178.0 36 356047.0 37 716519.0 38 1082394.0 39 423201.0 40 655.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.855374417755826 19.731608882668105 13.293329881494268 24.1196868180818 2 15.482919301467604 20.23808281564857 40.417237685131724 23.861760197752098 3 18.152844268934203 25.372474487592108 29.817382123427816 26.657299120045874 4 12.191894338423461 16.709076348445215 39.24076618096563 31.858263132165693 5 12.892849955371306 38.14433946342332 35.83602971070515 13.126780870500228 6 30.932708658604202 38.229003064602566 17.92528610303653 12.913002173756702 7 27.38991426297039 32.61858259130856 22.562280056897023 17.429223088824028 8 24.990958240849263 36.55179354567763 20.523937676684096 17.93331053678901 9 25.06326727705644 17.080627713474495 21.080575510357967 36.7755294991111 10-11 23.771679276354906 27.060716765500402 28.94445511123073 20.223148846913958 12-13 24.396044468692292 24.5021868018049 28.79212664336437 22.30964208613844 14-15 21.829206340274876 26.496935626623426 25.802065886964275 25.871792146137423 16-17 21.511121085373745 29.738481502599466 26.394392492743652 22.356004919283134 18-19 21.47948120769253 28.68099371629704 28.256724496029857 21.582800579980578 20-21 22.73054710122337 27.99367218006737 27.706252274694666 21.569528444014598 22-23 22.38128811483822 27.656283157517557 27.789029188472114 22.17339953917211 24-25 22.241112073715918 27.802350040491707 27.71167295740742 22.244864928384956 26-27 21.92867293708413 27.808437430700124 27.797886433536554 22.465003198679195 28-29 21.926403462384148 27.860299711467988 27.904904464360264 22.3083923617876 30-31 22.244263781943104 28.282356004486214 27.495354889891754 21.978025323678928 32-33 21.88482451101969 27.93725244370554 28.02761293756993 22.150310107704836 34-35 22.09279613384218 27.67223439219326 28.04953891442828 22.18543055953627 36-37 22.42817533692689 28.04884394517217 27.455108181430386 22.06787253647055 38-39 22.11212830072149 28.203628972337256 27.712294736750536 21.971947990190714 40-41 21.909094520576943 27.909251686122428 27.929564317969675 22.252089475330955 42-43 21.95445252359011 27.7768305875933 27.928042734901474 22.340674153915117 44-45 22.023918341701076 27.954980479778897 27.891539217529736 22.129561960990294 46-47 22.09358784932192 28.107265321768853 27.759579906824772 22.039566922084454 48-49 22.047223660340382 27.9514352773614 27.71942425078474 22.281916811513476 50-51 22.020569899646933 27.86091484211158 27.71194080007005 22.406574458171434 52-53 22.04683084961559 27.849930193009442 27.79015569206056 22.313083265314408 54-55 22.18221496557142 28.240511681827403 27.58519513733524 21.992078215265938 56-57 22.01235195385109 27.961147680391672 27.638170848495903 22.388329517261337 58-59 21.856773446467407 27.58663579118518 28.0773692327006 22.47922152964681 60-61 22.22653397798028 28.012802290600515 27.61785867063239 22.142805060786817 62-63 21.963058840823297 28.01114401734635 27.66030578782326 22.365491354007094 64-65 21.971074562268203 27.795664663464326 27.762786940759792 22.470473833507675 66-67 21.87695612907154 27.824342327480107 27.64486046339692 22.653841080051436 68-69 22.287707932956973 27.936241890453406 27.581931916412362 22.194118260177262 70-71 22.22840049841101 27.678292391462538 27.669247240504184 22.42405986962227 72-73 22.021098058019657 27.831500221631657 27.770924767866568 22.376476952482115 74-75 22.066942536546573 27.910843014073887 27.641356013476816 22.380858435902727 76-77 22.06109259089886 27.847453040402986 27.55000710965494 22.541447259043213 78-79 22.141764481306296 28.02033953137678 27.60257093717397 22.235325050142958 80-81 22.132817864883457 27.785844251576975 27.675577213449916 22.405760670089663 82-83 21.970088212277652 27.78206252274736 27.819878759030274 22.427970505944717 84-85 21.96930335061344 27.728552005763614 27.72066776611229 22.581476877510656 86-87 22.154763255694167 27.85483819775574 27.561548215165892 22.4288503313842 88-89 21.869905667159045 27.80592664392599 27.66450274673673 22.659664942178235 90-91 21.92077840166676 27.64592277663705 27.80779027745733 22.625508544238855 92-93 22.08582430785329 27.76078238701664 27.647983814281634 22.505409490848432 94-95 21.865969197663304 27.55479858249193 27.8082190192574 22.771013200587362 96-97 21.883483995665934 27.581931882808608 27.815795225222455 22.718788896303 98-99 21.875780371006943 27.626064837299484 27.716995496801843 22.781159294891722 100 21.988967989893315 27.57390861196 27.63817697018609 22.798946427960594 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 508.0 1 473.5 2 412.0 3 391.0 4 399.5 5 393.0 6 383.0 7 368.0 8 331.5 9 309.0 10 294.5 11 261.0 12 250.5 13 277.0 14 311.0 15 372.0 16 485.0 17 632.5 18 854.0 19 1227.0 20 1824.0 21 2668.0 22 4137.5 23 6596.0 24 9732.0 25 13366.0 26 18422.5 27 25533.5 28 34466.0 29 43875.5 30 52858.0 31 62858.0 32 73214.0 33 83124.5 34 93566.5 35 102237.5 36 108566.0 37 114476.0 38 119332.5 39 122117.5 40 123972.0 41 125115.5 42 124181.5 43 124027.5 44 122891.0 45 120105.0 46 118661.5 47 115631.5 48 112548.0 49 109630.0 50 106522.5 51 103205.5 52 97319.0 53 92254.0 54 88757.5 55 84864.5 56 78716.5 57 71299.5 58 64757.0 59 58710.5 60 52704.0 61 46624.5 62 40220.0 63 33725.0 64 28170.5 65 23480.0 66 19067.5 67 14705.5 68 11385.5 69 8887.0 70 6598.5 71 4945.0 72 3867.0 73 3043.0 74 2429.0 75 1835.0 76 1373.5 77 1057.0 78 778.5 79 584.5 80 412.5 81 212.0 82 128.5 83 101.0 84 60.0 85 29.0 86 31.0 87 35.0 88 24.5 89 14.5 90 10.5 91 4.5 92 5.0 93 8.5 94 8.5 95 5.5 96 1.5 97 1.0 98 1.0 99 0.5 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0592510779639881 2 0.041664083184457155 3 0.055513479402934565 4 0.0728976587566719 5 0.08576195147843753 6 0.08709473856222406 7 0.08920981371692877 8 0.10207410643869441 9 0.09688782626482943 10-11 0.10245076365802538 12-13 0.10676783486420348 14-15 0.10562337639008244 16-17 0.07352059185018082 18-19 0.0975687066228508 20-21 0.09123796797486479 22-23 0.05707805554477092 24-25 0.020498844821281944 26-27 0.18085341254338078 28-29 0.06461119993139045 30-31 0.07608475830485709 32-33 0.07101437266001702 34-35 0.021817145088940358 36-37 0.05260162936118356 38-39 0.07653384960482863 40-41 0.08173461659482172 42-43 0.07582399561455101 44-45 0.07401314359853671 46-47 0.07969197552075759 48-49 0.07693948045641583 50-51 0.07217331795026617 52-53 0.07588194287906348 54-55 0.06714639275381047 56-57 0.0696815855762305 58-59 0.0663785914990204 60-61 0.0633798205605007 62-63 0.06920352064400272 64-65 0.0657701452216396 66-67 0.06718985320219481 68-69 0.06708844548929802 70-71 0.059453893389781694 72-73 0.05786034361568911 74-75 0.06033758917359667 76-77 0.054455941825582214 78-79 0.051486144519318745 80-81 0.04656062703575984 82-83 0.05323904927082059 84-85 0.04327211977467786 86-87 0.041012176458692005 88-89 0.043518395648855804 90-91 0.03969387619103359 92-93 0.04227252946183796 94-95 0.041026663274820116 96-97 0.04450349914556758 98-99 0.04440209143267078 100 0.05261611617731168 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3451414.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.08265224525065 #Duplication Level Percentage of deduplicated Percentage of total 1 76.03632474356307 47.20536707061573 2 14.048696754852678 17.44360710261 3 4.561197600499827 8.495137333611073 4 2.009978051988623 4.99139073688784 5 1.0117760371291589 3.140686993158368 6 0.5921351385404868 2.205679193292142 7 0.36927430475742656 1.6047869771753414 8 0.26253541474083686 1.303911588433442 9 0.17818377678068856 0.9955909304658768 >10 0.8719869112757035 9.342174910160383 >50 0.0430113288967768 1.811981021486604 >100 0.01480665589266176 1.4210303028553462 >500 9.32810820713389E-5 0.0386558392478542 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 2.8973632256228897E-5 0.0 0.0 0.0 4 0.0 2.8973632256228897E-5 0.0 0.0 0.0 5 0.0 2.8973632256228897E-5 0.0 0.0 0.0 6 0.0 2.8973632256228897E-5 0.0 0.0 0.0 7 0.0 2.8973632256228897E-5 0.0 0.0 0.0 8 0.0 2.8973632256228897E-5 0.0 0.0 0.0 9 0.0 2.8973632256228897E-5 0.0 0.0 0.0 10-11 0.0 8.69208967686867E-5 0.0 0.0 0.0 12-13 0.0 1.4486816128114448E-4 0.0 0.0 0.0 14-15 0.0 1.4486816128114448E-4 0.0 0.0 0.0 16-17 0.0 1.4486816128114448E-4 0.0 0.0 0.0 18-19 0.0 1.4486816128114448E-4 0.0 0.0 0.0 20-21 0.0 1.4486816128114448E-4 0.0 1.4486816128114449E-5 0.0 22-23 0.0 1.4486816128114448E-4 0.0 7.243408064057224E-5 0.0 24-25 0.0 1.738417935373734E-4 0.0 1.1589452902491559E-4 0.0 26-27 0.0 1.738417935373734E-4 0.0 2.0281542579360227E-4 0.0 28-29 0.0 1.738417935373734E-4 0.0 8.257485193025236E-4 0.0 30-31 0.0 1.738417935373734E-4 0.0 0.0036941381126691843 0.0 32-33 0.0 1.738417935373734E-4 0.0 0.010952032992854523 0.0 34-35 0.0 1.8832860966548783E-4 0.0 0.021411514237353158 0.0 36-37 0.0 2.0281542579360227E-4 0.0 0.04051962471033611 0.0 38-39 0.0 2.0281542579360227E-4 0.0 0.07352059185018082 0.0 40-41 0.0 2.0281542579360227E-4 0.0 0.11180924687678731 0.0 42-43 0.0 2.0281542579360227E-4 0.0 0.1509960845033369 0.0 44-45 0.0 2.0281542579360227E-4 0.0 0.19680339710043476 0.0 46-47 0.0 2.607626903060601E-4 0.0 0.25553294968381074 0.0 48-49 0.0 2.607626903060601E-4 0.0 0.31902866477333636 0.0 50-51 0.0 2.607626903060601E-4 0.0 0.38957945931725374 0.0 52-53 0.0 2.607626903060601E-4 0.0 0.4611153573578829 0.0 54-55 0.0 2.607626903060601E-4 0.0 0.538214192791708 0.0 56-57 0.0 2.607626903060601E-4 0.0 0.6288726881214481 0.0 58-59 0.0 2.8973632256228896E-4 0.0 0.7342063281889684 0.0 60-61 0.0 2.8973632256228896E-4 0.0 0.8471455467237485 0.0 62-63 0.0 2.8973632256228896E-4 0.0 0.9644018364647069 0.0 64-65 0.0 2.8973632256228896E-4 0.0 1.0923059360598293 0.0 66-67 0.0 2.8973632256228896E-4 0.0 1.2368119269377709 0.0 68-69 0.0 2.8973632256228896E-4 0.0 1.4030916024562687 0.0 70-71 0.0 2.8973632256228896E-4 0.0 1.5874942849510374 0.0 72-73 0.0 2.8973632256228896E-4 0.0 1.7765762090551873 0.0 74-75 0.0 2.8973632256228896E-4 0.0 1.9720613058879635 0.0 76-77 0.0 2.8973632256228896E-4 0.0 2.186118500996983 0.0 78-79 0.0 3.1870995481851784E-4 0.0 2.4302503263879673 0.0 80-81 0.0 3.1870995481851784E-4 0.0 2.699429856864462 0.0 82-83 0.0 3.1870995481851784E-4 0.0 2.9787646454467644 0.0 84-85 0.0 3.1870995481851784E-4 0.0 3.2689500593090255 0.0 86-87 0.0 3.1870995481851784E-4 0.0 3.575433141315415 0.0 88 0.0 3.1870995481851784E-4 0.0 3.8241717742351398 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4615 0.0 36.87939 1 TCAACGC 4460 0.0 35.625366 4 GGTATCA 2630 0.0 35.57494 1 CAACGCA 4525 0.0 35.009735 5 ATCAACG 4605 0.0 34.80986 3 AACGCAG 4735 0.0 33.650715 6 TATCAAC 5435 0.0 29.83988 2 GTACATG 10125 0.0 28.882992 1 TACATGG 10215 0.0 27.749584 2 ACGCAGA 6000 0.0 26.164719 7 CGCAGAG 6195 0.0 25.287266 8 ACATGGG 10775 0.0 24.998552 3 CATGGGG 7425 0.0 21.77908 4 GCAGAGT 7530 0.0 20.118887 9 GAGTACA 7400 0.0 20.077156 1 AGTACAT 7570 0.0 18.38118 2 CTATACG 285 4.820322E-8 18.143679 4 GTATAGG 1235 0.0 17.51208 1 AGAGTAC 6215 0.0 16.889427 10-11 TCTATAC 1430 0.0 16.765314 3 >>END_MODULE