FastQCFastQC Report
Wed 25 May 2016
SRR1033012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3451414
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61150.1771737612468397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47820.13855190944928658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46200.053.3604281
GTATCAA87850.044.3734661
TCAACGC104750.036.926034
ATCAACG106050.036.385273
CAACGCA108150.035.72175
AACGCAG113550.034.022426
TATCAAC113800.033.9087682
ACGCAGA132750.028.9954517
CGCAGAG135150.028.4457768
GTACATG97450.027.6193771
TACATGG98500.025.9258752
GCAGAGT150650.025.2382899
ACATGGG100450.024.6109813
GAGTACT101400.021.94572612-13
AGAGTAC135650.021.41160210-11
CAGAGTA144900.020.98536110-11
CATGGGG71200.020.9250324
ATACCGC2301.01053956E-720.4339686
GTGGTAT22700.019.9344841
AGTACTT109000.019.48856512-13
Produced by FastQC (version 0.11.5)