##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033012_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3451414 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64097236668797 34.0 31.0 34.0 31.0 34.0 2 32.645874415529406 34.0 31.0 34.0 31.0 34.0 3 32.750660164210956 34.0 31.0 34.0 31.0 34.0 4 36.25975875394838 37.0 37.0 37.0 35.0 37.0 5 36.24544143356896 37.0 37.0 37.0 35.0 37.0 6 36.31438940677647 37.0 37.0 37.0 35.0 37.0 7 36.26098346938385 37.0 37.0 37.0 35.0 37.0 8 36.270157680301466 37.0 37.0 37.0 35.0 37.0 9 38.057716924136024 39.0 39.0 39.0 35.0 39.0 10-11 38.01770448285833 39.0 38.5 39.0 35.0 39.0 12-13 37.99492164660629 39.0 38.0 39.0 35.0 39.0 14-15 39.46393246362216 41.0 39.0 41.0 36.0 41.0 16-17 39.459747367310904 41.0 39.0 41.0 36.0 41.0 18-19 39.479594160538255 41.0 39.0 41.0 36.5 41.0 20-21 39.47228874890118 41.0 39.0 41.0 36.0 41.0 22-23 39.397913145163116 41.0 39.0 41.0 36.0 41.0 24-25 39.340761351724254 41.0 39.0 41.0 36.0 41.0 26-27 39.224708481799055 41.0 39.0 41.0 36.0 41.0 28-29 39.16943244131246 41.0 39.0 41.0 36.0 41.0 30-31 39.15464531348601 41.0 39.0 41.0 36.0 41.0 32-33 39.10588471855303 41.0 39.0 41.0 35.0 41.0 34-35 39.02045755739532 41.0 39.0 41.0 35.0 41.0 36-37 38.920736689368475 41.0 39.0 41.0 35.0 41.0 38-39 38.81872878767948 41.0 39.0 41.0 35.0 41.0 40-41 38.70855090116689 41.0 38.5 41.0 35.0 41.0 42-43 38.57858445842776 41.0 38.0 41.0 35.0 41.0 44-45 38.39888665920692 40.0 38.0 41.0 34.5 41.0 46-47 38.184894075297834 40.0 38.0 41.0 34.0 41.0 48-49 37.96567421352524 40.0 38.0 41.0 33.5 41.0 50-51 37.819967700194766 40.0 38.0 41.0 33.0 41.0 52-53 37.67823260263764 40.0 37.5 41.0 33.0 41.0 54-55 37.523918312900165 40.0 37.0 41.0 33.0 41.0 56-57 37.36401790686368 40.0 37.0 41.0 33.0 41.0 58-59 37.17960798096085 40.0 36.0 41.0 33.0 41.0 60-61 36.97921663410996 39.5 36.0 41.0 32.0 41.0 62-63 36.74423236389491 39.0 35.0 41.0 32.0 41.0 64-65 36.473581697240604 39.0 35.0 41.0 31.5 41.0 66-67 36.19174923668966 38.5 35.0 41.0 31.0 41.0 68-69 35.88769037849415 37.5 35.0 40.0 31.0 41.0 70-71 35.56411604055613 37.0 35.0 40.0 31.0 41.0 72-73 35.22113806109612 36.5 35.0 39.0 31.0 41.0 74-75 34.88089751041167 36.0 35.0 39.0 30.0 41.0 76-77 34.53258374683536 35.5 34.5 38.5 30.0 40.0 78-79 34.17826896454613 35.0 34.0 37.0 30.0 39.0 80-81 33.85438417413849 35.0 34.0 37.0 29.5 39.0 82-83 33.56313571770874 35.0 34.0 36.5 29.0 39.0 84-85 33.34500396069553 35.0 34.0 36.0 29.0 37.0 86-87 33.143603317365 35.0 34.0 36.0 29.0 37.0 88-89 32.934894220166 35.0 34.0 35.5 29.0 37.0 90-91 32.753505230030356 35.0 34.0 35.0 29.0 36.0 92-93 32.5797213837575 35.0 34.0 35.0 29.0 36.0 94-95 32.423459921064236 35.0 34.0 35.0 29.0 36.0 96-97 32.275769728001336 35.0 34.0 35.0 29.0 36.0 98-99 32.14761181938765 35.0 34.0 35.0 29.0 35.0 100 32.04032579111054 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21017075985408695 1101 2 0.15499538567866722 1101 3 0.1867541535009707 1101 4 0.1514154761636206 1101 5 0.14073142090391144 1101 6 0.20081266231983363 1101 7 0.24345272157044917 1101 8 0.20924025084244136 1101 9 0.28270634707950393 1101 10-11 0.26370593107317575 1101 12-13 0.2825494651690761 1101 14-15 0.36748139468053864 1101 16-17 0.36171631357689904 1101 18-19 0.3285213589300966 1101 20-21 0.30101662369322213 1101 22-23 0.3398181100561857 1101 24-25 0.3809978273755448 1101 26-27 0.3731055037639024 1101 28-29 0.40902936167917403 1101 30-31 0.43124299971921687 1101 32-33 0.3928203986753118 1101 34-35 0.3672789034283852 1101 36-37 0.3378319045455811 1101 38-39 0.307237468308017 1101 40-41 0.31917760606032886 1101 42-43 0.33009523130763796 1101 44-45 0.31671258119693846 1101 46-47 0.2805462681646915 1101 48-49 0.3288834334996338 1101 50-51 0.35882284344091886 1101 52-53 0.36997108185936867 1101 54-55 0.33508292482831337 1101 56-57 0.30902743509133046 1101 58-59 0.2981897985768711 1101 60-61 0.27574442273886746 1101 62-63 0.2887103575535832 1101 64-65 0.322452559473291 1101 66-67 0.35654526381881624 1101 68-69 0.3352212319570498 1101 70-71 0.30925111506192593 1101 72-73 0.3489428788378177 1101 74-75 0.35253251719016276 1101 76-77 0.34736367513990274 1101 78-79 0.2688249965342422 1101 80-81 0.32129415382046744 1101 82-83 0.31178089984173596 1101 84-85 0.3284734406889456 1101 86-87 0.34373493088577334 1101 88-89 0.3665833571419057 1101 90-91 0.40311278583287447 1101 92-93 0.38772256559674645 1101 94-95 0.4513295173969354 1101 96-97 0.4980941870830762 1101 98-99 0.4889594462983595 1101 100 0.48243278153999825 1102 1 0.21277811125335688 1102 2 0.13310903761954762 1102 3 0.15386731207443205 1102 4 0.12575704775973406 1102 5 0.14684592032875088 1102 6 0.16712189963293866 1102 7 0.16934008107022436 1102 8 0.22534567462746935 1102 9 0.22951081972849607 1102 10-11 0.2491584001001499 1102 12-13 0.29072699284425596 1102 14-15 0.3674670765438961 1102 16-17 0.35971708525894996 1102 18-19 0.3390069140826242 1102 20-21 0.3379840404805208 1102 22-23 0.34325698958058126 1102 24-25 0.3759610718765387 1102 26-27 0.4007102863210079 1102 28-29 0.418122314273603 1102 30-31 0.40858361265524934 1102 32-33 0.4093465464003003 1102 34-35 0.3778332183907054 1102 36-37 0.35822752869842134 1102 38-39 0.42149039660091603 1102 40-41 0.4408741405472014 1102 42-43 0.42420918570746124 1102 44-45 0.506826272816852 1102 46-47 0.4638708234508542 1102 48-49 0.40202842999402577 1102 50-51 0.42245315738805544 1102 52-53 0.3743170574522807 1102 54-55 0.39302971058444314 1102 56-57 0.33794812927202855 1102 58-59 0.39502571965604716 1102 60-61 0.36110459125490735 1102 62-63 0.35321962260456985 1102 64-65 0.36354221927062724 1102 66-67 0.3241959374076089 1102 68-69 0.3154016086606717 1102 70-71 0.2540049825497164 1102 72-73 0.3219577066252626 1102 74-75 0.3369648189812935 1102 76-77 0.2643209157560946 1102 78-79 0.2747172840933345 1102 80-81 0.2964362777035987 1102 82-83 0.2515602676501487 1102 84-85 0.28676636973798963 1102 86-87 0.26869795172437705 1102 88-89 0.33500976023506723 1102 90-91 0.25354534329164835 1102 92-93 0.30455859336008473 1102 94-95 0.3177212056531076 1102 96-97 0.27427361317472077 1102 98-99 0.1969284859949738 1102 100 0.19153527020059613 1103 1 0.17438457917757688 1103 2 0.07870973563625228 1103 3 0.12259517345205495 1103 4 0.10657146731894329 1103 5 0.10783357001197658 1103 6 0.14078493504870693 1103 7 0.17928130817762877 1103 8 0.15785621168143393 1103 9 0.2152079628428254 1103 10-11 0.22564191151113988 1103 12-13 0.2206172650161875 1103 14-15 0.3047514492414365 1103 16-17 0.32511104065974195 1103 18-19 0.28456030034605817 1103 20-21 0.35578207528850214 1103 22-23 0.3662739803475219 1103 24-25 0.29769321186687847 1103 26-27 0.3377551986418723 1103 28-29 0.38383095042282633 1103 30-31 0.3696724864547747 1103 32-33 0.33062136159207967 1103 34-35 0.35730658453157815 1103 36-37 0.33134944701104985 1103 38-39 0.3519045534115648 1103 40-41 0.41598492447283064 1103 42-43 0.41287757780601453 1103 44-45 0.3963968309431465 1103 46-47 0.3406825357854686 1103 48-49 0.3044401421868912 1103 50-51 0.34591040439687504 1103 52-53 0.32267601296469905 1103 54-55 0.3118233609927614 1103 56-57 0.31135775000187493 1103 58-59 0.29543924121337284 1103 60-61 0.2687444958304681 1103 62-63 0.21787542605559906 1103 64-65 0.3053826296389559 1103 66-67 0.26516475719022736 1103 68-69 0.24539292775133958 1103 70-71 0.26888889244263225 1103 72-73 0.3007123150912605 1103 74-75 0.2356661467936263 1103 76-77 0.26893779864515466 1103 78-79 0.21267708965797283 1103 80-81 0.20758276729554836 1103 82-83 0.24561905436416254 1103 84-85 0.24077555796399253 1103 86-87 0.2051473029863189 1103 88-89 0.19283240424265813 1103 90-91 0.2400547601025238 1103 92-93 0.2957349735254411 1103 94-95 0.3280341711289978 1103 96-97 0.3271061563249944 1103 98-99 0.41234113574021336 1103 100 0.440389200566905 1104 1 0.13231957221445612 1104 2 0.1277139699648231 1104 3 0.16631805310110082 1104 4 0.0835866827088978 1104 5 0.08660424243177545 1104 6 0.11349179528750852 1104 7 0.1389609768509743 1104 8 0.14409643717045384 1104 9 0.17659546365089795 1104 10-11 0.21921420608070719 1104 12-13 0.22576032922486888 1104 14-15 0.2641407206599169 1104 16-17 0.2536512013712624 1104 18-19 0.26970022946137107 1104 20-21 0.298761132177205 1104 22-23 0.26945195309496484 1104 24-25 0.29477909462126917 1104 26-27 0.2835653263347453 1104 28-29 0.3492001598754655 1104 30-31 0.30073765798271523 1104 32-33 0.32901903006058575 1104 34-35 0.28322086048439843 1104 36-37 0.31361059553420745 1104 38-39 0.2678522801229306 1104 40-41 0.27136248610961644 1104 42-43 0.2831494221505366 1104 44-45 0.2994829708380067 1104 46-47 0.28792300207309296 1104 48-49 0.25992300492867315 1104 50-51 0.2913282209550303 1104 52-53 0.3105575145852768 1104 54-55 0.2663198111341103 1104 56-57 0.2905886266221174 1104 58-59 0.24298926931928122 1104 60-61 0.25894696827562314 1104 62-63 0.2442541635858433 1104 64-65 0.26092389866921906 1104 66-67 0.23670434058784906 1104 68-69 0.27821944787368125 1104 70-71 0.25937734771387255 1104 72-73 0.21561987079785183 1104 74-75 0.24817018610207242 1104 76-77 0.2546182406075701 1104 78-79 0.23967832531602085 1104 80-81 0.2647625449501092 1104 82-83 0.24199763102243566 1104 84-85 0.3152217583229415 1104 86-87 0.30920534304701164 1104 88-89 0.29945157703218683 1104 90-91 0.3409698754335082 1104 92-93 0.3659137795387579 1104 94-95 0.41779502242102495 1104 96-97 0.4315820814948239 1104 98-99 0.4294296017843635 1104 100 0.43598321862540246 1105 1 0.06867302213060356 1105 2 0.07503381470765902 1105 3 0.107931339232195 1105 4 0.040215082250036005 1105 5 0.10193670665966437 1105 6 0.103700773860254 1105 7 0.1426342602392623 1105 8 0.09482727992244122 1105 9 0.15386965922554907 1105 10-11 0.13799280473694608 1105 12-13 0.1720387408075439 1105 14-15 0.24423824767026048 1105 16-17 0.2549875445728915 1105 18-19 0.23923000241424575 1105 20-21 0.21416316884974407 1105 22-23 0.23936052232781435 1105 24-25 0.21245060826753104 1105 26-27 0.18606311154081112 1105 28-29 0.20289996363237606 1105 30-31 0.2329557860323206 1105 32-33 0.20291667975254057 1105 34-35 0.24411194084535737 1105 36-37 0.19617698631953573 1105 38-39 0.11598087434534676 1105 40-41 0.17464692619050481 1105 42-43 0.17014642724276285 1105 44-45 0.20309754190984108 1105 46-47 0.2157774758723079 1105 48-49 0.20658256756338034 1105 50-51 0.21364018526200113 1105 52-53 0.2898378954416856 1105 54-55 0.23289474768910168 1105 56-57 0.1957219451613028 1105 58-59 0.17952814243969328 1105 60-61 0.14208886937527865 1105 62-63 0.20834300426474073 1105 64-65 0.23435689749477717 1105 66-67 0.23196768641945198 1105 68-69 0.16012135016320173 1105 70-71 0.13882334351255565 1105 72-73 0.18002676671359552 1105 74-75 0.15700478365281612 1105 76-77 0.09466153379614184 1105 78-79 0.058196707572321316 1105 80-81 0.087829783272241 1105 82-83 0.14439649567613344 1105 84-85 0.16891518205644473 1105 86-87 0.10887999405469628 1105 88-89 0.07985243893511296 1105 90-91 0.14640093779113528 1105 92-93 0.14302941241152212 1105 94-95 0.06775054943128822 1105 96-97 0.1270615913753872 1105 98-99 0.129981632807457 1105 100 0.11933138568090129 1106 1 0.05891200981484701 1106 2 0.039888815694048674 1106 3 0.07487607333020918 1106 4 0.03775756738376401 1106 5 0.08908970950609785 1106 6 0.09027180787654032 1106 7 0.07366743105345108 1106 8 0.09117200838395689 1106 9 0.10129095956655476 1106 10-11 0.10156338783307461 1106 12-13 0.0987099636466553 1106 14-15 0.14640125530772252 1106 16-17 0.1837083404469766 1106 18-19 0.18910459024503723 1106 20-21 0.20505105882522656 1106 22-23 0.18649858844398182 1106 24-25 0.1391718550617682 1106 26-27 0.1830130594031658 1106 28-29 0.12897160357263715 1106 30-31 0.1489690881770116 1106 32-33 0.1645926936183315 1106 34-35 0.10408714023661503 1106 36-37 0.1227763437583107 1106 38-39 0.0796705285459538 1106 40-41 0.08727409976658151 1106 42-43 0.09876224326733762 1106 44-45 0.0468001600650112 1106 46-47 0.057162082585385576 1106 48-49 0.06605893652879757 1106 50-51 0.031639649794307445 1106 52-53 0.033300962262423184 1106 54-55 0.11019514506249095 1106 56-57 0.04832178170274659 1106 58-59 0.05514745309550051 1106 60-61 0.09350504049947972 1106 62-63 0.04694952006103392 1106 64-65 0.03151257767964921 1106 66-67 0.09981989733735475 1106 68-69 0.07222644274274614 1106 70-71 0.09921564405084382 1106 72-73 0.11721358857194275 1106 74-75 0.11135475406117479 1106 76-77 0.08858538465429433 1106 78-79 0.09812075570805234 1106 80-81 0.029825245888140728 1106 82-83 0.09629529228296008 1106 84-85 0.10491974762305745 1106 86-87 0.06047301042872988 1106 88-89 0.11013736411054253 1106 90-91 0.07700784814257133 1106 92-93 0.0897503773806605 1106 94-95 0.13122953937013193 1106 96-97 0.09941807194354624 1106 98-99 0.13440628686714717 1106 100 0.13134981704239834 1107 1 0.1113915884443415 1107 2 0.04016941997805645 1107 3 0.0898648513513578 1107 4 0.058773326389299996 1107 5 0.05873310564012968 1107 6 0.07347245802778701 1107 7 0.0685923998285034 1107 8 0.06286696934638059 1107 9 0.09047996604687114 1107 10-11 0.10921660566516067 1107 12-13 0.08378964663945254 1107 14-15 0.14623860787079934 1107 16-17 0.11822314242343879 1107 18-19 0.13476689893971638 1107 20-21 0.11266225361699611 1107 22-23 0.20039308649521104 1107 24-25 0.17089292877603413 1107 26-27 0.13475107753811244 1107 28-29 0.14237936519163696 1107 30-31 0.1530233249399373 1107 32-33 0.16186642147127372 1107 34-35 0.11731599332918563 1107 36-37 0.16394870984549925 1107 38-39 0.2152628743597731 1107 40-41 0.21526842409821967 1107 42-43 0.25877288057780135 1107 44-45 0.250295892351204 1107 46-47 0.26758083670090116 1107 48-49 0.259353813298965 1107 50-51 0.23475204059835875 1107 52-53 0.22033257893628644 1107 54-55 0.22668246470142606 1107 56-57 0.2143086252023707 1107 58-59 0.24966560229849222 1107 60-61 0.26197767473020406 1107 62-63 0.2243984036002118 1107 64-65 0.23396385470503844 1107 66-67 0.23642620430141648 1107 68-69 0.22759638435630336 1107 70-71 0.2951629420424382 1107 72-73 0.272707753946861 1107 74-75 0.2879159830925957 1107 76-77 0.24312084004154144 1107 78-79 0.25415604994606866 1107 80-81 0.26032989875224644 1107 82-83 0.20165964300506545 1107 84-85 0.1911175906352085 1107 86-87 0.2166323639901293 1107 88-89 0.29017254653074076 1107 90-91 0.28553474325379113 1107 92-93 0.26266714910492794 1107 94-95 0.22398895125220264 1107 96-97 0.18927219352679714 1107 98-99 0.20993009492929104 1107 100 0.29367893857083516 1108 1 0.0049985796128524385 1108 2 0.025286082534137222 1108 3 0.044507384195590305 1108 4 0.027536071442703758 1108 5 0.07596932222166686 1108 6 0.026959689973622858 1108 7 0.05519149688381475 1108 8 0.056039441069607676 1108 9 0.0770941346769547 1108 10-11 0.04303321201580701 1108 12-13 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-0.19569230842557062 2214 84-85 -0.12306455594264065 2214 86-87 -0.14702117671662052 2214 88-89 -0.12072586442710076 2214 90-91 -0.051533833306088184 2214 92-93 -0.12689909508276287 2214 94-95 -0.1581876587679929 2214 96-97 -0.17553050242363355 2214 98-99 -0.14084643024602883 2214 100 -0.14860947570759464 2215 1 -0.184585423825105 2215 2 -0.17469572133704503 2215 3 -0.21659547966153525 2215 4 -0.13901710345266594 2215 5 -0.17928720150739252 2215 6 -0.16168238588287664 2215 7 -0.186446687136673 2215 8 -0.15881041380748684 2215 9 -0.20015179638926384 2215 10-11 -0.18656356674281227 2215 12-13 -0.18336197085996275 2215 14-15 -0.2728736524469966 2215 16-17 -0.30392116214689935 2215 18-19 -0.31233740145823674 2215 20-21 -0.3332530494907573 2215 22-23 -0.3677000621917159 2215 24-25 -0.36416960991865466 2215 26-27 -0.37043456025585897 2215 28-29 -0.4488555958697731 2215 30-31 -0.4717051659889293 2215 32-33 -0.3997021916979264 2215 34-35 -0.33922641424082656 2215 36-37 -0.4097645361103588 2215 38-39 -0.40982589016660853 2215 40-41 -0.3558791145267719 2215 42-43 -0.36445588663725914 2215 44-45 -0.4176831899290292 2215 46-47 -0.3913693297227496 2215 48-49 -0.28883854663472164 2215 50-51 -0.2954871121899387 2215 52-53 -0.26298171335517395 2215 54-55 -0.2927207841004673 2215 56-57 -0.2549880432222764 2215 58-59 -0.23281740351544045 2215 60-61 -0.2690053151225129 2215 62-63 -0.1979522276316814 2215 64-65 -0.24687474245351382 2215 66-67 -0.25766779055413025 2215 68-69 -0.23124960711098197 2215 70-71 -0.24239637855880858 2215 72-73 -0.20242005932773566 2215 74-75 -0.23417818980905736 2215 76-77 -0.2798681044642777 2215 78-79 -0.3166750971519363 2215 80-81 -0.2772220032612509 2215 82-83 -0.225450978928869 2215 84-85 -0.22108639085339377 2215 86-87 -0.2054933716302827 2215 88-89 -0.1929930143167411 2215 90-91 -0.1587960843953269 2215 92-93 -0.19135577118392177 2215 94-95 -0.1499526523227459 2215 96-97 -0.16956664911955954 2215 98-99 -0.13475062029451124 2215 100 -0.1517829764229397 2216 1 -0.3241038212449894 2216 2 -0.28030215767540767 2216 3 -0.2468726750689072 2216 4 -0.19442753423899006 2216 5 -0.20495783087395836 2216 6 -0.21525065485863593 2216 7 -0.21501026165847037 2216 8 -0.24856643771145315 2216 9 -0.33374647045864947 2216 10-11 -0.3821263882161148 2216 12-13 -0.3836852590297468 2216 14-15 -0.47103981482283785 2216 16-17 -0.4699788081057008 2216 18-19 -0.4591290083553119 2216 20-21 -0.4906144372681496 2216 22-23 -0.42154941764803056 2216 24-25 -0.4548577491145309 2216 26-27 -0.5400410198245069 2216 28-29 -0.4908356294375338 2216 30-31 -0.4629295376580487 2216 32-33 -0.5190896090126245 2216 34-35 -0.5679773797496637 2216 36-37 -0.49106434813862165 2216 38-39 -0.5029474019937368 2216 40-41 -0.45189589453788415 2216 42-43 -0.4178414106835078 2216 44-45 -0.5094877380321776 2216 46-47 -0.5095522566228468 2216 48-49 -0.5624051894903417 2216 50-51 -0.5393427978996286 2216 52-53 -0.5858703221900399 2216 54-55 -0.5175409913533571 2216 56-57 -0.6227219480206898 2216 58-59 -0.6111416092815816 2216 60-61 -0.5983409095371854 2216 62-63 -0.5598669453740115 2216 64-65 -0.6503108891682672 2216 66-67 -0.6421848038360451 2216 68-69 -0.593891045685254 2216 70-71 -0.5768083715819756 2216 72-73 -0.5917976231287554 2216 74-75 -0.6907069653599578 2216 76-77 -0.5904758281477527 2216 78-79 -0.6735333556159873 2216 80-81 -0.677457403509429 2216 82-83 -0.6219391401935255 2216 84-85 -0.7777644210024377 2216 86-87 -0.8208828564405763 2216 88-89 -0.9293096973414023 2216 90-91 -0.9269572329007545 2216 92-93 -0.8981703760657638 2216 94-95 -0.8238272900754495 2216 96-97 -0.9660817188007442 2216 98-99 -1.0023569520965765 2216 100 -1.0374454364716819 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16.0 3 6.0 4 13.0 5 26.0 6 91.0 7 243.0 8 499.0 9 919.0 10 1610.0 11 2689.0 12 4283.0 13 6568.0 14 7715.0 15 10001.0 16 11919.0 17 16955.0 18 19460.0 19 10229.0 20 7082.0 21 6891.0 22 7166.0 23 7551.0 24 8808.0 25 11159.0 26 13851.0 27 17765.0 28 23175.0 29 29628.0 30 37835.0 31 48200.0 32 61306.0 33 81007.0 34 112598.0 35 171021.0 36 314346.0 37 686077.0 38 1177180.0 39 533919.0 40 1607.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.69160644770443 19.884953520526622 13.554208660515782 23.869231371253164 2 15.774520749421159 20.51012987194039 39.81462549272687 23.900723885911585 3 18.19211465546166 25.61485277781158 30.122759041918528 26.070273524808236 4 12.279417730027932 16.97593964048538 38.87176500585283 31.872877623633855 5 12.534761155585688 37.926007052190265 35.86366151804625 13.675570274177801 6 30.61668673574757 38.41424900881147 17.618122669794275 13.350941585646686 7 27.0342242338937 32.70007596886377 22.648891150119923 17.6168086471226 8 25.226299341542948 35.76366937262017 20.470347991917485 18.5396832939194 9 25.372354634940926 16.10545706774093 21.30150135567625 37.22068694164189 10-11 23.921857172079257 26.42892821981122 29.01306948662542 20.636145121484105 12-13 24.983064911946233 23.923499180335945 29.113314137336175 21.980121770381647 14-15 22.066895694301706 25.71891778502829 26.721582498479602 25.492604022190395 16-17 21.11217476688506 29.183965157418317 27.213364165185872 22.490495910510752 18-19 20.939355588662913 27.644218371801806 30.05445909164924 21.361966947886046 20-21 22.170702260204735 26.932360746163965 29.711454410121302 21.18548258351 22-23 21.761593853639752 26.686336896950912 29.790287066859804 21.76178218254953 24-25 21.590525843665468 26.904925814923242 29.705496487190008 21.799051854221283 26-27 21.341110253812285 26.933319116917176 29.813070545971225 21.912500083299314 28-29 21.35142276758435 26.97975057708349 29.870644059075037 21.79818259625712 30-31 21.612630779884164 27.366684920076146 29.45775784389568 21.56292645614401 32-33 21.346453946121795 27.034557430664652 29.944292397260945 21.67469622595261 34-35 21.575780245429844 26.893846985612274 29.82095164474618 21.7094211242117 36-37 21.924115739230356 27.25026612281227 29.201307058498344 21.624311079459027 38-39 21.66239691905984 27.398162028664192 29.337903247770335 21.601537804505632 40-41 21.549473309687937 27.158960906284285 29.453123743720504 21.838442040307278 42-43 21.654921721937733 27.11007720314051 29.245520821321346 21.98948025360041 44-45 21.707697193092454 27.287960818377627 29.128467347006183 21.875874641523733 46-47 21.8887389342455 27.517721722169526 28.747507543285156 21.84603180029982 48-49 21.921319783717628 27.497874784074007 28.440285633656238 22.14051979855213 50-51 21.915597491347025 27.532295459194405 28.274194286747402 22.277912762711168 52-53 21.959173834260394 27.52881862332366 28.330113397001927 22.181894145414024 54-55 22.034997829874943 27.860581199473604 28.128645245108235 21.975775725543212 56-57 21.81689881306618 27.613682392202154 28.24855262220064 22.320866172531026 58-59 21.725269701055858 27.22531982543966 28.64946077173008 22.399949701774403 60-61 22.05099127488038 27.58404236640403 28.215131537393077 22.149834821322507 62-63 21.78331837328121 27.568077895030846 28.320218901586426 22.32838483010152 64-65 21.83202304910393 27.36930429093699 28.37599314367966 22.422679516279416 66-67 21.71140581802125 27.39850971225127 28.309672499445153 22.580411970282324 68-69 22.130142249192215 27.497545929081696 28.165114063593755 22.20719775813233 70-71 22.11454006149656 27.269154295523933 28.220040345800452 22.396265297179053 72-73 21.904953821000223 27.4297594328573 28.2370245289855 22.428262217156977 74-75 21.98635716403897 27.47201613486204 28.11127688884118 22.43034981225781 76-77 21.959854714618416 27.44391139399678 28.017603799486242 22.578630091898567 78-79 22.026754831498046 27.574915672243318 28.085836703449658 22.31249279280898 80-81 21.964997534343894 27.407984089999054 28.103612026838853 22.523406348818195 82-83 21.832925722262576 27.41148475217817 28.223239712685622 22.532349812873633 84-85 21.705611091570006 27.41101183456983 28.11663567453803 22.766741399322132 86-87 21.88875255149714 27.462946249425958 28.006565442189483 22.641735756887414 88-89 21.57573947129689 27.409295664219606 28.099909281901493 22.91505558258201 90-91 21.560354191735353 27.31599886537185 28.184933957745823 22.938712985146974 92-93 21.664639467624113 27.40344776432493 28.06787456437917 22.86403820367179 94-95 21.41621412070873 27.16331841088208 28.24153336081977 23.178934107589416 96-97 21.387126645125697 27.16249426321084 28.22979781005317 23.22058128161029 98-99 21.33498232171267 27.23234192180643 28.129482770659752 23.303192985821152 100 21.46013199227911 27.14302022301584 28.019298756973228 23.377549027731824 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 45.0 1 35.0 2 97.5 3 180.0 4 138.5 5 113.5 6 283.0 7 642.0 8 1077.0 9 1294.5 10 1071.0 11 894.0 12 1018.0 13 1192.5 14 1433.5 15 1687.5 16 1955.0 17 2315.5 18 2702.5 19 3177.5 20 3867.0 21 4800.5 22 6326.5 23 8750.5 24 11814.0 25 15318.5 26 19961.0 27 26600.0 28 35214.5 29 44349.0 30 53164.0 31 62888.0 32 72917.0 33 82512.0 34 92368.0 35 100695.0 36 107220.5 37 113607.5 38 118709.5 39 121488.5 40 123182.5 41 124460.0 42 123982.0 43 123855.5 44 122768.5 45 119757.5 46 118487.5 47 115646.5 48 111936.5 49 109400.0 50 106450.0 51 102738.5 52 96557.0 53 91218.5 54 87292.0 55 83445.0 56 77665.5 57 69604.5 58 63382.5 59 58144.0 60 52210.5 61 45682.5 62 39434.5 63 33279.5 64 27511.5 65 22816.0 66 18510.0 67 14147.5 68 10832.5 69 8421.0 70 6199.5 71 4630.5 72 3577.0 73 2760.0 74 2155.5 75 1609.0 76 1151.5 77 892.0 78 638.5 79 457.5 80 314.0 81 137.5 82 71.0 83 42.5 84 24.5 85 13.5 86 8.0 87 3.5 88 3.0 89 3.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04357634291336826 2 0.24135035669438668 3 0.0028973632256228898 4 0.0027235214320855163 5 1.1589452902491557E-4 6 5.794726451245779E-5 7 0.0 8 3.476835870747467E-4 9 0.0 10-11 4.635781160996623E-4 12-13 0.0 14-15 3.187099548185179E-4 16-17 1.4486816128114448E-4 18-19 4.635781160996623E-4 20-21 2.8973632256228894E-5 22-23 1.5935497740925895E-4 24-25 0.0013327870837865291 26-27 1.4486816128114448E-4 28-29 2.0281542579360224E-4 30-31 1.1589452902491557E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.243408064057224E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.7384179353737336E-4 70-71 4.346044838434334E-5 72-73 2.1730224192171674E-4 74-75 1.5935497740925895E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.692089676868668E-5 84-85 0.0 86-87 2.607626903060601E-4 88-89 0.0018253388321424204 90-91 0.0018253388321424204 92-93 5.215253806121202E-4 94-95 4.346044838434335E-4 96-97 1.7384179353737336E-4 98-99 1.4486816128114448E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3451414.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.75686209408082 #Duplication Level Percentage of deduplicated Percentage of total 1 74.59276776930488 44.574297368061494 2 14.774319031705973 17.65733889823215 3 4.866138089971128 8.723554283194757 4 2.1200425177279456 5.06748353461827 5 1.0986765252403377 3.2826730802395385 6 0.6372886595543665 2.284942232586715 7 0.41157327974923164 1.721602940370837 8 0.2787318643668016 1.33249132641544 9 0.19983283330350649 1.0747244755428371 >10 0.9522713683219091 9.945480534808093 >50 0.04999470479061124 2.01221614147204 >100 0.017732707805844735 1.7258211641656855 >500 4.366025722914435E-4 0.17059960873526267 >1k 1.4553418878719698E-4 0.24950736043129074 >5k 4.8511396262398996E-5 0.1772670511255191 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6115 0.1771737612468397 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4782 0.13855190944928658 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 4.346044838434335E-5 0.0 0.0 0.0 12-13 0.0 1.0140771289680113E-4 0.0 0.0 0.0 14-15 0.0 1.1589452902491559E-4 0.0 2.8973632256228897E-5 0.0 16-17 0.0 1.4486816128114448E-4 0.0 2.8973632256228897E-5 0.0 18-19 0.0 1.4486816128114448E-4 0.0 2.8973632256228897E-5 0.0 20-21 0.0 1.4486816128114448E-4 0.0 4.346044838434335E-5 0.0 22-23 0.0 1.4486816128114448E-4 0.0 1.0140771289680113E-4 0.0 24-25 0.0 1.4486816128114448E-4 0.0 1.4486816128114448E-4 0.0 26-27 0.0 1.4486816128114448E-4 0.0 2.3178905804983118E-4 0.0 28-29 0.0 1.738417935373734E-4 0.0 8.547221515587523E-4 0.0 30-31 0.0 1.738417935373734E-4 0.0 0.0037086249287972984 0.0 32-33 0.0 1.738417935373734E-4 0.0 0.010995493441238867 0.0 34-35 0.0 1.8832860966548783E-4 0.0 0.021469461501865614 0.0 36-37 0.0 2.3178905804983118E-4 0.0 0.040650006055489143 0.0 38-39 0.0 2.3178905804983118E-4 0.0 0.07360751274694952 0.0 40-41 0.0 2.3178905804983118E-4 0.0 0.11198308867032469 0.0 42-43 0.0 2.3178905804983118E-4 0.0 0.15122787356138673 0.0 44-45 0.0 2.3178905804983118E-4 0.0 0.19715108068750953 0.0 46-47 0.0 2.607626903060601E-4 0.0 0.2557792255579887 0.0 48-49 0.0 2.607626903060601E-4 0.0 0.3196226242345891 0.0 50-51 0.0 2.607626903060601E-4 0.0 0.39024585285914704 0.0 52-53 0.0 2.607626903060601E-4 0.0 0.4620859740384665 0.0 54-55 0.0 2.607626903060601E-4 0.0 0.5397497953012881 0.0 56-57 0.0 2.607626903060601E-4 0.0 0.6310891709890497 0.0 58-59 0.0 3.476835870747468E-4 0.0 0.736698060563004 0.0 60-61 0.0 3.476835870747468E-4 0.0 0.8500139363171153 0.0 62-63 0.0 3.476835870747468E-4 0.0 0.9678786723354544 0.0 64-65 0.0 3.476835870747468E-4 0.0 1.096304297311189 0.0 66-67 0.0 3.476835870747468E-4 0.0 1.2411579717762053 0.0 68-69 0.0 3.476835870747468E-4 0.0 1.4076694363527529 0.0 70-71 0.0 3.476835870747468E-4 0.0 1.5927095387571586 0.0 72-73 0.0 3.476835870747468E-4 0.0 1.7827620795418921 0.0 74-75 0.0 3.476835870747468E-4 0.0 1.979710344803608 0.0 76-77 0.0 3.476835870747468E-4 0.0 2.1951003269964136 0.0 78-79 0.0 3.476835870747468E-4 0.0 2.440956083506644 0.0 80-81 0.0 3.476835870747468E-4 0.0 2.711772624205615 0.0 82-83 0.0 3.476835870747468E-4 0.0 2.9929472384361886 0.0 84-85 0.0 3.476835870747468E-4 0.0 3.284914530682207 0.0 86-87 0.0 3.476835870747468E-4 0.0 3.5938313977981196 0.0 88 0.0 3.476835870747468E-4 0.0 3.843671028743582 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4620 0.0 53.360428 1 GTATCAA 8785 0.0 44.373466 1 TCAACGC 10475 0.0 36.92603 4 ATCAACG 10605 0.0 36.38527 3 CAACGCA 10815 0.0 35.7217 5 AACGCAG 11355 0.0 34.02242 6 TATCAAC 11380 0.0 33.908768 2 ACGCAGA 13275 0.0 28.995451 7 CGCAGAG 13515 0.0 28.445776 8 GTACATG 9745 0.0 27.619377 1 TACATGG 9850 0.0 25.925875 2 GCAGAGT 15065 0.0 25.238289 9 ACATGGG 10045 0.0 24.610981 3 GAGTACT 10140 0.0 21.945726 12-13 AGAGTAC 13565 0.0 21.411602 10-11 CAGAGTA 14490 0.0 20.985361 10-11 CATGGGG 7120 0.0 20.925032 4 ATACCGC 230 1.01053956E-7 20.433968 6 GTGGTAT 2270 0.0 19.934484 1 AGTACTT 10900 0.0 19.488565 12-13 >>END_MODULE