FastQCFastQC Report
Wed 25 May 2016
SRR1033011_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033011_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270002
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12220.45258924007970314No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5150.1907393278568307No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3450.12777683128273123No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCACG301.3714745E-462.646272
CGTCAAG301.3714745E-462.646277
GTGTAAT405.6676794E-447.0021321
ACGTCAC500.001702438637.5877655
GCACCTA250.001705501237.58079554-55
CAACGTA352.1651125E-433.61971722-23
CCTAAGC953.7405895E-529.6855581
CTCTAAA804.770027E-429.3763331
GGGTATT650.00615571328.9136666
CGTATCA500.001766513228.18037494
GATATGC1057.417304E-526.8484046
TCACGTA700.00883826826.8484022
GTATCAT909.5374236E-426.09293794
GTACAAA2051.0713848E-925.2206571
ACTATGG950.001293231524.7655228
GCCGTAT952.0340212E-424.71962794
GGTTATA1151.3729704E-424.5228521
GTACATG14050.024.0865021
TATGCCT1201.8186243E-423.5272458
GTGCTAC1201.8324074E-423.5010641
Produced by FastQC (version 0.11.5)