FastQCFastQC Report
Wed 25 May 2016
SRR1033011_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033011_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270002
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18050.6685135665661736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17760.6577729053858861No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT14710.5448107791794136No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8940.3311086584543818No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT6000.2222205761438804TruSeq Adapter, Index 1 (95% over 21bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5770.21370212072503164No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4750.175924622780572No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT3310.12259168450604069RNA PCR Primer, Index 1 (95% over 23bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3100.11481396434100488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2800.10370293553381087No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCGT250.005232611756.3975566
CCCGTAG250.005232611756.3975568
CCTACGC352.9100289E-453.801632
GCCTACG405.603178E-447.1113781
GTCAGCG250.001701590537.59837376-77
AATACCG651.4109876E-436.152285
ATACCGC651.4109876E-436.152286
GCTAGCC702.1811499E-433.5699737
GTATTAG753.2238796E-431.4075851
TACCGCA753.2697897E-431.3319767
CTAATAC902.5874037E-531.3319743
CTAGCCT600.004156279431.3319728
GTCTACA953.7004895E-529.7324772
CAACCTA404.7787785E-429.37372624-25
ATGTAGG804.7725235E-429.3737267
ATGCATT804.7725235E-429.3737267
GGTATCA20650.029.2022111
CTGTACT650.006147247728.9218254
TAACACA1005.3102398E-528.1987784
ACCGCAT856.804218E-427.6458598
Produced by FastQC (version 0.11.5)