##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033011_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	270002
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.576354989963036	34.0	31.0	34.0	31.0	34.0
2	32.54989222302057	34.0	31.0	34.0	31.0	34.0
3	32.623969452078136	34.0	31.0	34.0	31.0	34.0
4	36.1868282457167	37.0	35.0	37.0	35.0	37.0
5	36.177457944755965	37.0	35.0	37.0	35.0	37.0
6	36.26470915030259	37.0	37.0	37.0	35.0	37.0
7	36.21087991940801	37.0	37.0	37.0	35.0	37.0
8	36.22695757809201	37.0	37.0	37.0	35.0	37.0
9	37.999818519862814	39.0	38.0	39.0	35.0	39.0
10-11	37.95977066836542	39.0	38.0	39.0	35.0	39.0
12-13	37.93571899467411	39.0	38.0	39.0	35.0	39.0
14-15	39.31433841230806	41.0	39.0	41.0	36.0	41.0
16-17	39.309094006711064	41.0	39.0	41.0	36.0	41.0
18-19	39.388236013066575	41.0	39.0	41.0	36.0	41.0
20-21	39.39854889963778	41.0	39.0	41.0	36.0	41.0
22-23	39.30106258472159	41.0	39.0	41.0	36.0	41.0
24-25	39.20335404922926	41.0	39.0	41.0	35.5	41.0
26-27	39.04259412893238	41.0	39.0	41.0	35.0	41.0
28-29	38.93273753527752	40.0	38.5	41.0	35.0	41.0
30-31	38.780468292827464	40.0	38.0	41.0	35.0	41.0
32-33	38.5472496500026	40.0	38.0	41.0	35.0	41.0
34-35	38.332558647713725	40.0	38.0	41.0	35.0	41.0
36-37	38.13664343227087	40.0	38.0	41.0	34.0	41.0
38-39	37.90486922319094	40.0	37.0	41.0	33.5	41.0
40-41	37.70001148139643	40.0	37.0	41.0	33.0	41.0
42-43	37.41887467500241	40.0	37.0	41.0	33.0	41.0
44-45	36.96530766438767	40.0	37.0	41.0	32.5	41.0
46-47	36.353714046562615	40.0	36.0	41.0	30.5	41.0
48-49	35.79115339886371	40.0	36.0	41.0	27.5	41.0
50-51	35.445617069503186	40.0	35.0	41.0	25.5	41.0
52-53	35.20627810164369	40.0	35.0	41.0	24.0	41.0
54-55	34.97770572069837	39.0	35.0	41.0	23.0	41.0
56-57	34.76346656691432	39.0	35.0	41.0	23.0	41.0
58-59	34.556169954296635	39.0	35.0	41.0	22.0	41.0
60-61	34.296714468781715	38.0	35.0	41.0	22.0	41.0
62-63	34.061229176080175	37.5	35.0	40.5	21.0	41.0
64-65	33.74724816853208	37.0	35.0	40.0	19.5	41.0
66-67	33.38375826845727	36.5	34.0	40.0	17.0	41.0
68-69	32.99127043503381	36.0	34.0	39.0	10.0	41.0
70-71	32.569253190717106	35.0	34.0	39.0	4.0	41.0
72-73	32.190689328227194	35.0	34.0	38.5	2.0	40.5
74-75	31.883810119925037	35.0	33.5	37.0	2.0	40.0
76-77	31.572890200813326	35.0	33.0	37.0	2.0	39.0
78-79	31.254344412263613	35.0	33.0	36.5	2.0	39.0
80-81	30.98434270857253	35.0	33.0	36.0	2.0	38.0
82-83	30.75324071673543	35.0	33.0	35.5	2.0	37.0
84-85	30.598360382515686	35.0	33.0	35.0	2.0	37.0
86-87	30.44190413404345	35.0	33.0	35.0	2.0	36.5
88-89	30.291966355804774	35.0	33.0	35.0	2.0	36.0
90-91	30.143852638128607	35.0	33.0	35.0	2.0	36.0
92-93	29.98734453818861	35.0	33.0	35.0	2.0	35.5
94-95	29.858439937481943	35.0	32.5	35.0	2.0	35.0
96-97	29.73437233798268	35.0	32.0	35.0	2.0	35.0
98-99	29.615023222050205	35.0	32.0	35.0	2.0	35.0
100	29.52411463618788	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.16871234298363902
1101	2	0.17459538720839163
1101	3	0.17413338585605231
1101	4	0.1953263464366728
1101	5	0.18078020426845143
1101	6	0.13931980027881963
1101	7	0.2192131634086465
1101	8	0.19510748601418726
1101	9	0.17919611390487233
1101	10-11	0.2653930615753737
1101	12-13	0.30885541733893973
1101	14-15	0.3521656233205235
1101	16-17	0.3651839233486527
1101	18-19	0.33671670057275094
1101	20-21	0.21905256510250126
1101	22-23	0.24166264080496092
1101	24-25	0.311383663894766
1101	26-27	0.32677434833836827
1101	28-29	0.5085999912433579
1101	30-31	0.33517022252895146
1101	32-33	0.16545060627058206
1101	34-35	0.001560771957947793
1101	36-37	0.0011017735287168762
1101	38-39	-0.10143649217014428
1101	40-41	-0.03862089598981555
1101	42-43	0.14992578279493785
1101	44-45	-0.14902212376092905
1101	46-47	-0.45638163314936264
1101	48-49	-0.34877657526233463
1101	50-51	-0.248198366933309
1101	52-53	-0.7371189578999378
1101	54-55	-0.6196572971089225
1101	56-57	-0.705260152732734
1101	58-59	-0.6555234011792663
1101	60-61	-0.6320212610757565
1101	62-63	-0.591612642841973
1101	64-65	-0.5467358835534171
1101	66-67	-0.5761049879038183
1101	68-69	-0.7472899360598362
1101	70-71	-1.0387550581947487
1101	72-73	-1.0416723643468728
1101	74-75	-0.8908342032593417
1101	76-77	-0.7995116517090324
1101	78-79	-0.8377227900063993
1101	80-81	-0.8340650766958149
1101	82-83	-0.708107681680147
1101	84-85	-0.6982976737580238
1101	86-87	-0.6778036339888516
1101	88-89	-0.6035933338183632
1101	90-91	-0.6725833011029145
1101	92-93	-0.6264964766049701
1101	94-95	-0.6581648410821366
1101	96-97	-0.3640700829905583
1101	98-99	-0.5578723386742332
1101	100	-0.8850274948877832
1102	1	0.33055762021353274
1102	2	0.33118096687089604
1102	3	0.22826443998710744
1102	4	0.1659597016854093
1102	5	0.26296297875891383
1102	6	0.280235864271809
1102	7	0.21340558067798554
1102	8	0.08125694908672898
1102	9	0.35134163220423886
1102	10-11	0.4066378566663147
1102	12-13	0.4147616193989947
1102	14-15	0.6682953625271821
1102	16-17	0.7209805483760476
1102	18-19	0.6107688592403022
1102	20-21	0.6716604984796604
1102	22-23	0.6695609533186513
1102	24-25	0.6219797629523995
1102	26-27	0.648382939177722
1102	28-29	0.5877146088195104
1102	30-31	0.5875809172473438
1102	32-33	0.5364236503205433
1102	34-35	0.7283304410353111
1102	36-37	0.8375580522926853
1102	38-39	0.9448598041261533
1102	40-41	0.7639212911677618
1102	42-43	0.47542435829351604
1102	44-45	0.5046596645362484
1102	46-47	0.635772651312621
1102	48-49	0.7612935540385735
1102	50-51	0.8997306271495304
1102	52-53	0.899030505172604
1102	54-55	0.839689623776458
1102	56-57	0.9095765774886075
1102	58-59	0.7430082665831748
1102	60-61	0.7081058482821234
1102	62-63	0.8377649596125494
1102	64-65	0.896064905401218
1102	66-67	0.7102197984209724
1102	68-69	0.985287315748181
1102	70-71	1.0817796777246045
1102	72-73	1.0069250496351572
1102	74-75	1.0751421281016036
1102	76-77	0.9435302067174476
1102	78-79	1.0035000896780204
1102	80-81	0.6678525213756288
1102	82-83	0.7657581960318893
1102	84-85	0.8311785480258891
1102	86-87	0.954236690359167
1102	88-89	0.8798605342508914
1102	90-91	0.7999676522172656
1102	92-93	0.897610470578897
1102	94-95	0.7511601643967154
1102	96-97	0.8929428470992953
1102	98-99	1.0448561294388483
1102	100	1.0262151086625124
1103	1	0.061401003111711816
1103	2	0.007885490150762564
1103	3	0.2293494027159042
1103	4	0.15416561043926436
1103	5	0.16789214396205665
1103	6	0.20917470827914997
1103	7	0.15471612198852824
1103	8	0.09543171394642513
1103	9	0.3754918097790707
1103	10-11	0.3116684932038041
1103	12-13	0.23502466431957458
1103	14-15	0.3581638400668936
1103	16-17	0.49814169266047514
1103	18-19	0.3273789361243402
1103	20-21	0.36991987482123534
1103	22-23	0.31915311660944923
1103	24-25	0.208542616638546
1103	26-27	0.18523264452058186
1103	28-29	0.2648211146931416
1103	30-31	0.3173822675966349
1103	32-33	0.24115377559861884
1103	34-35	0.2132775588591329
1103	36-37	0.38457830753212363
1103	38-39	0.32759406631247145
1103	40-41	0.3754343848726336
1103	42-43	0.3741091336906237
1103	44-45	0.3605547587875009
1103	46-47	0.6730906858909265
1103	48-49	0.6356564311411717
1103	50-51	0.7207000686669147
1103	52-53	0.7878101172398857
1103	54-55	0.7079993455108351
1103	56-57	0.9688826696714159
1103	58-59	0.9057799951083325
1103	60-61	1.0504623248851743
1103	62-63	0.9698650259591872
1103	64-65	1.0851762506769447
1103	66-67	1.0066136633079878
1103	68-69	1.147258981829438
1103	70-71	1.2079905039354273
1103	72-73	1.3623440091637065
1103	74-75	1.220452981631631
1103	76-77	1.2794783782820573
1103	78-79	1.2955267844444407
1103	80-81	1.3829366255008253
1103	82-83	1.3291582662812687
1103	84-85	1.4148416631958582
1103	86-87	1.3000353868427936
1103	88-89	1.1598823896152126
1103	90-91	1.4103177283207664
1103	92-93	1.5037377780760686
1103	94-95	1.4516714238120834
1103	96-97	1.283919703829575
1103	98-99	1.3421398198732248
1103	100	1.4464469316340605
1104	1	0.08710154297729389
1104	2	0.08069022040159979
1104	3	0.09741653702123898
1104	4	-0.10035714103894122
1104	5	0.03634122398702999
1104	6	0.037681080162066394
1104	7	0.18635151072847833
1104	8	0.1762058885396911
1104	9	0.13646724038614622
1104	10-11	0.13210626175938955
1104	12-13	0.07881988265297224
1104	14-15	0.2283573837152133
1104	16-17	-0.011190269585661383
1104	18-19	0.19931327084101014
1104	20-21	0.1781757784533866
1104	22-23	0.17056154145060987
1104	24-25	0.21583263830661537
1104	26-27	0.23128281162183129
1104	28-29	0.2549235789775466
1104	30-31	0.1717067011050304
1104	32-33	0.22657740481746202
1104	34-35	0.3298481270034017
1104	36-37	0.464056070211619
1104	38-39	0.5335913486217692
1104	40-41	0.5970216631421223
1104	42-43	0.5680016747314198
1104	44-45	0.4914152384231869
1104	46-47	0.5336401001184043
1104	48-49	0.4761794029726758
1104	50-51	0.4289119016391112
1104	52-53	0.6295744126602045
1104	54-55	0.6931034624449879
1104	56-57	0.6717803155636588
1104	58-59	0.6198862788499326
1104	60-61	0.6998949151382092
1104	62-63	0.5707721470433356
1104	64-65	0.6397577630350924
1104	66-67	0.6952911762537468
1104	68-69	0.6855233323380929
1104	70-71	0.5085266969649211
1104	72-73	0.724468949484681
1104	74-75	0.6444347657588523
1104	76-77	0.6577515429226963
1104	78-79	0.7009291128443067
1104	80-81	0.6473862931346019
1104	82-83	0.5832807092648551
1104	84-85	0.2510971261214152
1104	86-87	0.34944226921085786
1104	88-89	0.4123338200753821
1104	90-91	0.4788476045556642
1104	92-93	0.5355274141258661
1104	94-95	0.6542826104146009
1104	96-97	0.8738973012440994
1104	98-99	0.8231380203212773
1104	100	0.7245034155299059
1105	1	0.1616117512676638
1105	2	-0.09246575677582314
1105	3	0.007466719189388016
1105	4	0.11781155353725836
1105	5	0.11509973089566472
1105	6	0.07482275885870138
1105	7	0.09317766045006692
1105	8	0.07498914281892155
1105	9	0.07071485157745627
1105	10-11	0.13413073415919285
1105	12-13	0.04396389860126959
1105	14-15	0.008181402413221406
1105	16-17	0.1255389529344484
1105	18-19	0.2745865230579554
1105	20-21	0.2202359970551555
1105	22-23	0.3244042581619624
1105	24-25	0.17311936409200257
1105	26-27	0.16918065997544574
1105	28-29	0.29084851195341344
1105	30-31	0.0784641081305324
1105	32-33	0.4214663853632885
1105	34-35	0.5243418370467055
1105	36-37	0.6636264283610629
1105	38-39	0.5498455591119011
1105	40-41	0.38771046495693184
1105	42-43	0.49351552638468377
1105	44-45	0.4208989807755614
1105	46-47	0.4022968678368386
1105	48-49	0.5641425568875746
1105	50-51	0.35485883227773485
1105	52-53	0.4786601348022259
1105	54-55	0.6634787975656309
1105	56-57	0.6273828452948749
1105	58-59	0.5202162437911539
1105	60-61	0.6219235120997908
1105	62-63	0.7644031362507278
1105	64-65	0.8704238797601889
1105	66-67	0.6636386018397715
1105	68-69	0.6925522730131419
1105	70-71	0.8797853757303002
1105	72-73	0.9307142234243315
1105	74-75	0.8751421281016079
1105	76-77	0.8908236540108945
1105	78-79	0.6829872691652028
1105	80-81	0.5638639173870246
1105	82-83	0.6593479396216289
1105	84-85	0.6693551862025302
1105	86-87	0.5847210208434959
1105	88-89	0.6881226425130009
1105	90-91	0.7221898744394899
1105	92-93	0.6538782768467044
1105	94-95	0.7064308196673714
1105	96-97	0.8163046704611219
1105	98-99	0.9093860439687624
1105	100	1.0800612625086643
1106	1	0.06718867434458531
1106	2	0.21240603809597047
1106	3	0.15297953970220846
1106	4	0.0595423227680314
1106	5	0.0518305001264352
1106	6	0.030784297320245457
1106	7	0.13721612198852995
1106	8	0.04902760435738429
1106	9	-0.03152873816613777
1106	10-11	0.06332945210791507
1106	12-13	0.13947671911409287
1106	14-15	0.33718781266963305
1106	16-17	0.0905389529344518
1106	18-19	0.251317292288725
1106	20-21	0.2936013816705412
1106	22-23	0.3160709248286224
1106	24-25	0.3421898769125207
1106	26-27	0.25982168561646546
1106	28-29	0.14459851195341855
1106	30-31	0.004008979925409051
1106	32-33	0.09528048792738275
1106	34-35	0.3946302985851702
1106	36-37	0.29897899246363124
1106	38-39	0.468467353983705
1106	40-41	0.4569412341877026
1106	42-43	0.2943488597180135
1106	44-45	0.485674621801202
1106	46-47	0.4112071242470847
1106	48-49	0.3350399927850063
1106	50-51	0.25857678099568204
1106	52-53	0.42132039121248965
1106	54-55	0.42097879756563117
1106	56-57	0.27148540939744237
1106	58-59	0.27098547456038347
1106	60-61	0.7115068454331208
1106	62-63	0.6221595465071417
1106	64-65	0.6437892643755774
1106	66-67	0.6612027044038769
1106	68-69	0.8038343242951953
1106	70-71	0.6367405039354281
1106	72-73	0.7301693516294563
1106	74-75	0.8411036665631428
1106	76-77	0.733964679651919
1106	78-79	0.5849103460882787
1106	80-81	0.634152378925485
1106	82-83	0.8638671703908614
1106	84-85	0.897752622099965
1106	86-87	0.7948812772537543
1106	88-89	1.009180334820691
1106	90-91	0.8755232077728223
1106	92-93	0.9421795588979833
1106	94-95	0.6971679991545479
1106	96-97	0.7291251832816315
1106	98-99	0.7911809157636362
1106	100	0.5908304932778954
1107	1	0.0017618450762952875
1107	2	-0.15369152287964027
1107	3	-0.14763021639535623
1107	4	0.0358228105729097
1107	5	0.02091586598009343
1107	6	0.1108452729299998
1107	7	0.17422831711048303
1107	8	0.05603979947933624
1107	9	0.10292248134606297
1107	10-11	-0.07596932837989101
1107	12-13	0.010818182528730347
1107	14-15	0.1538036663281659
1107	16-17	-0.04519275438262582
1107	18-19	0.09747582887408868
1107	20-21	0.029241625572979046
1107	22-23	0.25082702238960053
1107	24-25	0.15057402325398073
1107	26-27	0.1655533929335391
1107	28-29	0.08962899975828975
1107	30-31	0.18562483358393678
1107	32-33	0.23256707329323945
1107	34-35	0.1066120059022353
1107	36-37	0.21797289490265115
1107	38-39	0.3910588173983385
1107	40-41	0.31913635613891955
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2216	76-77	-0.33353532034808353
2216	78-79	-0.32879933133107997
2216	80-81	-0.4050411694616116
2216	82-83	-0.3606892812220437
2216	84-85	-0.6643038295129386
2216	86-87	-0.7018122711333419
2216	88-89	-0.7076745038889847
2216	90-91	-0.6162509857755616
2216	92-93	-0.644110763682658
2216	94-95	-0.6570255492325465
2216	96-97	-0.9664393328474006
2216	98-99	-0.8855529552041084
2216	100	-0.8815082163995243
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	1.0
5	11.0
6	36.0
7	88.0
8	125.0
9	198.0
10	328.0
11	593.0
12	1002.0
13	1536.0
14	1674.0
15	2233.0
16	3107.0
17	4612.0
18	5246.0
19	2262.0
20	971.0
21	791.0
22	770.0
23	875.0
24	1065.0
25	1495.0
26	2010.0
27	2458.0
28	2038.0
29	2499.0
30	3180.0
31	4011.0
32	4971.0
33	6459.0
34	9049.0
35	14465.0
36	27759.0
37	58398.0
38	76452.0
39	27193.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.77282327570236	18.61137254611343	13.652465187973824	22.963338990210392
2	17.246232051420105	20.981690313429453	37.70065092031146	24.071426714838978
3	19.092575788440527	25.57417729957962	31.216874386562715	24.116372525417137
4	13.045652190016222	18.114578626508347	35.32878021318677	33.51098897028867
5	12.825508053673875	37.260232369509744	34.43727986192644	15.476979714889943
6	30.323849452967018	36.74195005962918	16.725061295842252	16.209139191561544
7	25.852030725698327	33.860119554669964	21.47576684617151	18.812082873460195
8	26.159903111469955	32.61395328165451	20.207332565434942	21.01881104144059
9	26.350175183887526	14.47952237390834	20.575032777534982	38.59526966466915
10-11	24.52842417934881	25.91472561009485	27.162137637546806	22.394712573009528
12-13	26.49739631558285	23.689268968378013	28.209605854771446	21.603728861267694
14-15	23.842415982103837	24.234079747557423	26.991281546062623	24.932222724276116
16-17	21.033362715831736	27.295723735379738	28.482011244361154	23.188902304427376
18-19	21.26162729328279	24.83106512765717	32.154755268971726	21.752552310088312
20-21	22.079651261842507	23.59075117962089	32.771609099191856	21.557988459344745
22-23	21.608358456604027	24.183524566484692	32.56420322812424	21.643913748787046
24-25	21.949381200265925	24.053466963705354	32.657959210884506	21.339192625144214
26-27	21.58335864178784	23.72760201776283	33.315864326930914	21.37317501351842
28-29	21.515290684108574	23.99009633297654	32.97210010333295	21.522512879581928
30-31	22.09205857734387	24.0837104910334	32.608462159539556	21.21576877208317
32-33	21.702246650024815	23.91426730172369	32.86105288108977	21.522433167161726
34-35	21.271138732305687	23.3879749038896	33.370493551899614	21.970392811905096
36-37	22.280575699439265	23.53334419745039	32.396982244575966	21.789097858534383
38-39	21.674654261820283	23.966674320930956	32.37753794416338	21.981133473085386
40-41	21.69117578976413	23.577276422538393	32.45833819441744	22.27320959328004
42-43	22.427796831134582	23.645010036962688	31.716616913948787	22.210576217953943
44-45	22.40909326597581	24.049266301731098	31.34736038992304	22.194280042370057
46-47	22.51853689972667	24.175006111065844	30.382923089458597	22.92353389974889
48-49	22.608721416878392	24.233524196117067	29.257931422730206	23.89982296427434
50-51	22.82464574336486	24.475189072673537	28.96626691654136	23.73389826742024
52-53	22.63316568025422	24.910741401915544	28.76904615521367	23.687046762616575
54-55	22.754090710439183	24.62611388063792	28.387011948059644	24.232783460863253
56-57	22.43613010273998	24.78629787927497	27.944978185346773	24.832593832638278
58-59	22.19909482151984	25.038703417011725	28.290901548877418	24.471300212591018
60-61	22.218168754305523	25.208331790134885	28.016644321153176	24.556855134406412
62-63	21.937615276923875	25.82388278605344	27.9436818986526	24.294820038370084
64-65	21.46132250872216	25.42018207272539	28.340160443255975	24.778334975296477
66-67	21.617432463463235	25.179443115236182	28.06627358315864	25.13685083814194
68-69	21.944363169025298	25.69212706619605	27.97434083577468	24.389168929003965
70-71	22.380204591077103	25.27610906585877	27.60683254198117	24.736853801082955
72-73	22.390884461325072	24.498658335817897	28.242725476434305	24.86773172642273
74-75	22.241625478471335	25.005324064214697	27.791800385554843	24.961250071759125
76-77	22.495203739231563	24.973333530862735	27.910533996044474	24.62092873386123
78-79	22.842053021829468	24.6190768957267	27.965348404826628	24.5735216776172
80-81	22.642610054740338	24.835927141280433	27.775349812223617	24.746112991755616
82-83	21.9232521361032	24.901574438613192	28.065266424939168	25.109907000344446
84-85	22.494277820164296	24.585003074051304	27.66923948711491	25.251479618669492
86-87	22.73037037037037	25.015555555555558	27.22851851851852	25.025555555555556
88-89	22.15938131958488	24.944258020548602	27.19912294347282	25.697237716393694
90-91	22.52282449674994	24.464897498101816	27.08994611011315	25.922331895035093
92-93	22.319156737617547	24.83564753943533	27.023248234252716	25.821947488694402
94-95	22.111193004418535	24.54583165122834	27.518620439334963	25.824354905018165
96-97	21.837511712919582	24.670649367965304	27.294158169784556	26.19768074933056
98-99	21.79932333709257	24.614307698290567	27.177071238493117	26.409297726123743
100	21.739838964155823	24.72907608091792	26.792023762786943	26.739061192139317
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	57.0
1	43.0
2	91.0
3	165.5
4	107.0
5	61.0
6	130.0
7	254.0
8	397.0
9	451.5
10	329.5
11	175.5
12	142.5
13	166.0
14	170.5
15	163.5
16	176.5
17	188.5
18	193.0
19	222.5
20	248.5
21	265.0
22	311.0
23	417.5
24	596.0
25	789.5
26	1076.5
27	1481.0
28	1992.0
29	2486.5
30	2781.0
31	3052.0
32	3471.5
33	4033.0
34	4645.5
35	5487.0
36	6241.5
37	7041.0
38	7681.5
39	8315.5
40	9252.5
41	9669.0
42	9201.0
43	8589.0
44	8295.0
45	8149.5
46	8306.5
47	8959.0
48	10112.0
49	10715.5
50	10550.5
51	10472.0
52	9939.5
53	9089.0
54	8760.0
55	8400.5
56	7315.0
57	6485.0
58	6109.0
59	5604.5
60	4985.5
61	4379.0
62	3869.0
63	2980.5
64	2672.0
65	2779.5
66	2373.0
67	1699.0
68	1263.5
69	990.0
70	676.0
71	435.5
72	253.5
73	140.5
74	70.0
75	65.0
76	81.0
77	44.5
78	22.5
79	50.5
80	54.5
81	32.5
82	28.5
83	12.0
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04592558573640195
2	0.255924030192369
3	0.0025925733883452717
4	0.0014814705076258694
5	3.7036762690646736E-4
6	0.0
7	0.0
8	3.7036762690646736E-4
9	0.0
10-11	0.001111102880719402
12-13	0.0
14-15	0.0
16-17	0.0
18-19	5.55551440359701E-4
20-21	0.0
22-23	0.0
24-25	0.0012962866941726358
26-27	0.0
28-29	3.7036762690646736E-4
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	1.8518381345323368E-4
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	3.7036762690646736E-4
70-71	0.0
72-73	5.55551440359701E-4
74-75	5.55551440359701E-4
76-77	0.0
78-79	0.0
80-81	0.0
82-83	3.7036762690646736E-4
84-85	0.0
86-87	7.407352538129347E-4
88-89	0.002962941015251739
90-91	0.0025925733883452717
92-93	0.001111102880719402
94-95	0.001111102880719402
96-97	3.7036762690646736E-4
98-99	1.8518381345323368E-4
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	270002.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.08180988897706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.926	27.04228067963541
2	9.44	7.00104570703887
3	4.032	4.485415724170665
4	2.6	3.8565082284536145
5	1.8769999999999998	3.4801278580804973
6	1.337	2.97470278929374
7	1.08	2.8033848276066657
8	0.894	2.6520910432596394
9	0.747	2.493010078835928
>10	4.802	32.614193433553105
>50	0.214	5.194790747346797
>100	0.045	2.761111564333232
>500	0.003	0.7679642828007149
>1k	0.003	1.8733730355911211
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1805	0.6685135665661736	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1776	0.6577729053858861	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	1471	0.5448107791794136	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	894	0.3311086584543818	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	600	0.2222205761438804	TruSeq Adapter, Index 1 (95% over 21bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	577	0.21370212072503164	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	475	0.175924622780572	No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT	331	0.12259168450604069	RNA PCR Primer, Index 1 (95% over 23bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	310	0.11481396434100488	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	280	0.10370293553381087	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.7036762690646736E-4	0.0
10-11	0.0	0.0	0.0	7.407352538129347E-4	0.0
12-13	0.0	0.0	0.0	7.407352538129347E-4	0.0
14-15	0.0	0.0	0.0	7.407352538129347E-4	0.0
16-17	0.0	0.0	0.0	9.259190672661684E-4	0.0
18-19	0.0	0.0	0.0	0.001666654321079103	0.0
20-21	0.0	0.0	0.0	0.0031481248287049727	0.0
22-23	0.0	0.0	0.0	0.0049999629632373095	0.0
24-25	0.0	0.0	0.0	0.007407352538129347	0.0
26-27	0.0	0.0	0.0	0.015370256516618396	0.0
28-29	0.0	0.0	0.0	0.0357404759964741	0.0
30-31	0.0	0.0	0.0	0.08222161317323576	0.0
32-33	0.0	0.0	0.0	0.16277657202539242	0.0
34-35	0.0	0.0	0.0	0.3033310864363967	0.0
36-37	0.0	0.0	0.0	0.5057369945407811	0.0
38-39	0.0	0.0	0.0	0.7622165761735098	0.0
40-41	0.0	0.0	0.0	1.0438811564358783	0.0
42-43	0.0	0.0	0.0	1.289990444515226	0.0
44-45	0.0	0.0	0.0	1.5494329671632063	0.0
46-47	0.0	0.0	0.0	1.8290605254775891	0.0
48-49	0.0	0.0	0.0	2.127762016577655	0.0
50-51	0.0	0.0	0.0	2.4362782497907425	0.0
52-53	0.0	0.0	0.0	2.7133132347167797	0.0
54-55	0.0	0.0	0.0	3.024977592758572	0.0
56-57	0.0	0.0	0.0	3.390715624328709	0.0
58-59	0.0	0.0	0.0	3.7496018548010754	0.0
60-61	0.0	0.0	0.0	4.120895400774809	0.0
62-63	0.0	0.0	0.0	4.501077769794298	0.0
64-65	0.0	0.0	0.0	4.909222894645225	0.0
66-67	0.0	0.0	0.0	5.362182502351834	0.0
68-69	0.0	0.0	0.0	5.8608825119813925	0.0
70-71	0.0	0.0	0.0	6.397360019555411	0.0
72-73	0.0	0.0	0.0	6.971985392700795	0.0
74-75	0.0	0.0	0.0	7.5516107288094165	0.0
76-77	0.0	0.0	0.0	8.137717498388902	0.0
78-79	0.0	0.0	0.0	8.817156909948816	0.0
80-81	0.0	0.0	0.0	9.549003340715995	0.0
82-83	0.0	0.0	0.0	10.307145872993534	0.0
84-85	0.0	0.0	0.0	11.07769572077244	0.0
86-87	0.0	0.0	0.0	11.838060458811416	0.0
88	0.0	0.0	0.0	12.465092851164066	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCCGT	25	0.0052326117	56.397556	6
CCCGTAG	25	0.0052326117	56.397556	8
CCTACGC	35	2.9100289E-4	53.80163	2
GCCTACG	40	5.603178E-4	47.111378	1
GTCAGCG	25	0.0017015905	37.598373	76-77
AATACCG	65	1.4109876E-4	36.15228	5
ATACCGC	65	1.4109876E-4	36.15228	6
GCTAGCC	70	2.1811499E-4	33.569973	7
GTATTAG	75	3.2238796E-4	31.407585	1
TACCGCA	75	3.2697897E-4	31.331976	7
CTAATAC	90	2.5874037E-5	31.331974	3
CTAGCCT	60	0.0041562794	31.331972	8
GTCTACA	95	3.7004895E-5	29.732477	2
CAACCTA	40	4.7787785E-4	29.373726	24-25
ATGTAGG	80	4.7725235E-4	29.373726	7
ATGCATT	80	4.7725235E-4	29.373726	7
GGTATCA	2065	0.0	29.202211	1
CTGTACT	65	0.0061472477	28.921825	4
TAACACA	100	5.3102398E-5	28.198778	4
ACCGCAT	85	6.804218E-4	27.645859	8
>>END_MODULE