FastQCFastQC Report
Wed 25 May 2016
SRR1033010_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033010_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4309001
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55100.036.0946961
GGTATCA34100.034.8835561
TCAACGC56700.032.9163444
ATCAACG59300.031.869523
CAACGCA60300.031.1078365
AACGCAG62800.029.4883126
GTACATG135950.028.8084831
TATCAAC69100.028.2341162
TACATGG137050.027.8192842
ACATGGG144300.025.6070983
ACGCAGA74950.024.2066127
CGCAGAG79850.022.447728
CATGGGG105900.021.9298234
TACACCG6550.020.0969875
GAGTACA96300.019.8711381
AGTACAT97700.018.0924512
GCAGAGT96650.018.0628039
ACCGTCC4405.456968E-1217.1076158
GTGTAAG19400.015.9954621
AGAGTAC80650.015.87602710-11
Produced by FastQC (version 0.11.5)