##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033010_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4309001
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55699824622923	31.0	31.0	34.0	30.0	34.0
2	31.705042537701893	33.0	31.0	34.0	30.0	34.0
3	31.8052200498445	34.0	31.0	34.0	30.0	34.0
4	35.264616787046464	37.0	35.0	37.0	33.0	37.0
5	35.323468247048446	37.0	35.0	37.0	33.0	37.0
6	35.46978058255266	37.0	35.0	37.0	35.0	37.0
7	35.43509667321962	37.0	35.0	37.0	35.0	37.0
8	35.436838840371585	37.0	36.0	37.0	35.0	37.0
9	37.12705659618088	39.0	38.0	39.0	35.0	39.0
10-11	37.104182732842254	39.0	38.0	39.0	35.0	39.0
12-13	37.04293547390683	39.0	38.0	39.0	34.0	39.0
14-15	38.446674414788944	41.0	39.0	41.0	34.5	41.0
16-17	38.43562788683502	41.0	39.0	41.0	34.0	41.0
18-19	38.43142876968467	41.0	39.0	41.0	34.0	41.0
20-21	38.436995605245855	40.5	39.0	41.0	34.0	41.0
22-23	38.391790927874	40.0	39.0	41.0	34.0	41.0
24-25	38.38501708400625	40.0	39.0	41.0	34.0	41.0
26-27	38.27790316131279	40.0	38.0	41.0	34.0	41.0
28-29	38.285110284263105	40.0	38.0	41.0	34.0	41.0
30-31	38.4301982060343	40.5	39.0	41.0	34.0	41.0
32-33	38.41657764758003	41.0	39.0	41.0	34.0	41.0
34-35	38.37630926518699	41.0	39.0	41.0	34.0	41.0
36-37	38.2986693435439	40.5	38.0	41.0	34.0	41.0
38-39	38.20580385105504	40.0	38.0	41.0	33.5	41.0
40-41	38.124849936214915	40.0	38.0	41.0	33.0	41.0
42-43	37.998710373935864	40.0	38.0	41.0	33.0	41.0
44-45	37.85098529798438	40.0	38.0	41.0	33.0	41.0
46-47	37.70953685088493	40.0	38.0	41.0	33.0	41.0
48-49	37.578951362508384	40.0	37.5	41.0	32.5	41.0
50-51	37.435306814735014	40.0	37.0	41.0	32.0	41.0
52-53	37.26715101713831	40.0	37.0	41.0	32.0	41.0
54-55	37.09230921505936	40.0	36.0	41.0	31.0	41.0
56-57	36.89788468371207	40.0	36.0	41.0	31.0	41.0
58-59	36.67997570666611	39.0	35.0	41.0	31.0	41.0
60-61	36.43642714401784	39.0	35.0	41.0	31.0	41.0
62-63	36.165221358732566	39.0	35.0	41.0	30.0	41.0
64-65	35.86942356244522	38.0	35.0	40.0	30.0	41.0
66-67	35.552348676642225	37.5	35.0	40.0	30.0	41.0
68-69	35.21814975211191	37.0	34.5	40.0	29.5	41.0
70-71	34.87059645611593	36.5	34.0	39.0	29.0	41.0
72-73	34.51149767660764	36.0	34.0	39.0	29.0	41.0
74-75	34.15078669046491	35.5	34.0	38.5	29.0	40.0
76-77	33.78793286425322	35.0	34.0	37.5	28.5	39.5
78-79	33.41027177761156	35.0	34.0	37.0	28.0	39.0
80-81	33.07463806576048	35.0	33.0	36.5	27.5	39.0
82-83	32.87816781662386	35.0	33.0	36.0	27.5	37.5
84-85	32.6838098900418	35.0	33.5	36.0	27.5	37.0
86-87	32.536403333394446	35.0	34.0	35.5	27.5	37.0
88-89	32.342261234100434	35.0	33.0	35.0	27.0	36.0
90-91	32.15469316437847	35.0	33.0	35.0	27.0	36.0
92-93	31.95289905943396	35.0	33.0	35.0	27.0	36.0
94-95	31.767802792340962	35.0	33.0	35.0	26.0	36.0
96-97	31.603387072780905	35.0	33.0	35.0	25.5	35.0
98-99	31.435595164633288	35.0	33.0	35.0	25.0	35.0
100	31.297782246975576	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.010774054793341037
1101	2	0.02701154494603486
1101	3	0.05478981992322929
1101	4	-0.045234955469247495
1101	5	-0.03568233590301162
1101	6	0.1176683092049231
1101	7	0.16813658290082145
1101	8	0.2268860863593929
1101	9	0.2704668584784784
1101	10-11	0.2503038102260433
1101	12-13	0.25909297230486317
1101	14-15	0.345813500057794
1101	16-17	0.3359274773395384
1101	18-19	0.33240630031939844
1101	20-21	0.3131701436531742
1101	22-23	0.3523186449382081
1101	24-25	0.31513922904934333
1101	26-27	0.2559808305350728
1101	28-29	0.27531611662799804
1101	30-31	0.3128449740755528
1101	32-33	0.2795195550957601
1101	34-35	0.24976683657656196
1101	36-37	0.27818377379567494
1101	38-39	0.2981243797207469
1101	40-41	0.25636767459408816
1101	42-43	0.31138687883830585
1101	44-45	0.288947449441622
1101	46-47	0.26442383085647947
1101	48-49	0.26092816007853514
1101	50-51	0.3160530357055933
1101	52-53	0.2569616397224408
1101	54-55	0.23163547738779755
1101	56-57	0.2981170402928299
1101	58-59	0.32706642537941377
1101	60-61	0.3475279951803074
1101	62-63	0.2739183823547009
1101	64-65	0.32556807351512873
1101	66-67	0.2190426618166441
1101	68-69	0.263786186531604
1101	70-71	0.19269429117050407
1101	72-73	0.20853567882208068
1101	74-75	0.21531311803448006
1101	76-77	0.181860298756078
1101	78-79	0.23109937182405815
1101	80-81	0.2019751609071747
1101	82-83	0.21681861598563046
1101	84-85	0.23308851143345777
1101	86-87	0.1831675685799965
1101	88-89	0.24741134467807058
1101	90-91	0.26308107912504397
1101	92-93	0.24660797363838327
1101	94-95	0.2129068375530565
1101	96-97	0.28763586083089265
1101	98-99	0.32415963608409726
1101	100	0.3706501334449257
1102	1	-0.0821225204383822
1102	2	-0.007052799384471342
1102	3	-0.006803297563426014
1102	4	-0.16111364092967762
1102	5	-0.082769813979084
1102	6	0.06806390009481333
1102	7	0.040905463084150995
1102	8	0.11253343436415264
1102	9	0.13458842906393187
1102	10-11	0.13097106579823503
1102	12-13	0.0817351097758987
1102	14-15	0.16073585414364544
1102	16-17	0.1426390800587498
1102	18-19	0.09551264897707767
1102	20-21	0.14455208729747682
1102	22-23	0.18656958270625523
1102	24-25	0.09786509352426265
1102	26-27	0.04883106929785441
1102	28-29	0.0747233704078738
1102	30-31	0.11990095850796223
1102	32-33	0.11321743375562221
1102	34-35	0.09212354689905311
1102	36-37	0.11197638943239241
1102	38-39	0.1599831448114557
1102	40-41	0.09154357811600278
1102	42-43	0.17407107184568815
1102	44-45	0.10521020372718937
1102	46-47	0.08134366289037587
1102	48-49	0.030418372724639653
1102	50-51	0.1029879744466129
1102	52-53	0.03861947280613265
1102	54-55	0.09779165257438649
1102	56-57	0.047425076292874735
1102	58-59	0.00806654060005485
1102	60-61	-0.044685969562173966
1102	62-63	-0.041934378460041444
1102	64-65	7.738703939281777E-4
1102	66-67	-0.033005705250737094
1102	68-69	-0.07686909889554983
1102	70-71	-0.07862600942736009
1102	72-73	-0.043289608269368784
1102	74-75	-0.10496859644875656
1102	76-77	-0.11757672035774647
1102	78-79	-0.15659410284661135
1102	80-81	-0.06500612213874746
1102	82-83	-0.12460256669030656
1102	84-85	-0.05835027439696461
1102	86-87	-0.09251799427286755
1102	88-89	-0.060333403180742096
1102	90-91	-0.02510336352708009
1102	92-93	0.0074780815617216945
1102	94-95	0.03151465207341886
1102	96-97	-0.009583800932496445
1102	98-99	-0.005630022222668174
1102	100	-0.017619764520791392
1103	1	-0.007175915812855038
1103	2	0.061438692364383485
1103	3	0.017850829802398493
1103	4	-8.489845026105058E-5
1103	5	0.016260947245989144
1103	6	0.06304086992621905
1103	7	0.13795231938071595
1103	8	0.13949293117891415
1103	9	0.23308893347027038
1103	10-11	0.22143983041439697
1103	12-13	0.22925937365351245
1103	14-15	0.3086886253537813
1103	16-17	0.2979684509595728
1103	18-19	0.28678445873998726
1103	20-21	0.31018262043341593
1103	22-23	0.29646366297548354
1103	24-25	0.2482063309974265
1103	26-27	0.2173674702955779
1103	28-29	0.19500425836469049
1103	30-31	0.23910335232356061
1103	32-33	0.21910295396072144
1103	34-35	0.20935417682724022
1103	36-37	0.2908135745705209
1103	38-39	0.22219187885514913
1103	40-41	0.23679273412459168
1103	42-43	0.174930257054406
1103	44-45	0.1544642088704009
1103	46-47	0.1978287740810245
1103	48-49	0.2054980386793659
1103	50-51	0.16033532080993496
1103	52-53	0.1599081792013095
1103	54-55	0.17698861582974246
1103	56-57	0.19832076864589965
1103	58-59	0.19262313633615946
1103	60-61	0.1479264379936751
1103	62-63	0.11392089416865048
1103	64-65	0.13269013277933084
1103	66-67	0.129791092091061
1103	68-69	0.1228413505778363
1103	70-71	0.08697622261971816
1103	72-73	0.05098145874749349
1103	74-75	0.08432111483813287
1103	76-77	0.1241267755339095
1103	78-79	0.10726327815018522
1103	80-81	0.17064516979685607
1103	82-83	0.1616417317815717
1103	84-85	0.1234773037748198
1103	86-87	0.08867872588159997
1103	88-89	0.07852901907878618
1103	90-91	0.11345063147758339
1103	92-93	0.06637443992156022
1103	94-95	0.034772750161828014
1103	96-97	0.08153527257303494
1103	98-99	0.05725407298550067
1103	100	0.009319371193921455
1104	1	-0.07098581638305035
1104	2	0.021901788998409444
1104	3	0.024109697910233763
1104	4	0.036608860463438475
1104	5	-0.03456077321684603
1104	6	0.06728618629939831
1104	7	0.06706979166037286
1104	8	0.12616184121978335
1104	9	0.1727787344785341
1104	10-11	0.11974779415593417
1104	12-13	0.11896567978217121
1104	14-15	0.19338430279014318
1104	16-17	0.18740802991134586
1104	18-19	0.15950362254252326
1104	20-21	0.21250248893718293
1104	22-23	0.2179695687067067
1104	24-25	0.1543189997020633
1104	26-27	0.06853425950210834
1104	28-29	0.09640218005160506
1104	30-31	0.14966681041121888
1104	32-33	0.13875048337913398
1104	34-35	0.17414883743092702
1104	36-37	0.1854297115660728
1104	38-39	0.17829681040463896
1104	40-41	0.2299755263132539
1104	42-43	0.1520983945010812
1104	44-45	0.16705618232645492
1104	46-47	0.16569438411375614
1104	48-49	0.17077880122052136
1104	50-51	0.1593308821660031
1104	52-53	0.13004046361399446
1104	54-55	0.1787266454023495
1104	56-57	0.19818758744194298
1104	58-59	0.2545802402077939
1104	60-61	0.2551733922876096
1104	62-63	0.28500505266568155
1104	64-65	0.26263740312801076
1104	66-67	0.21574363100063465
1104	68-69	0.21300070246930147
1104	70-71	0.21056709644594918
1104	72-73	0.2140642207467991
1104	74-75	0.21689233050800283
1104	76-77	0.1471417791301235
1104	78-79	0.1814023551555337
1104	80-81	0.17056562314146362
1104	82-83	0.22179656621199229
1104	84-85	0.14748558678647328
1104	86-87	0.2145632534816002
1104	88-89	0.20114691896223746
1104	90-91	0.22445005873625945
1104	92-93	0.2619351502180791
1104	94-95	0.3323135155935759
1104	96-97	0.32089664370662874
1104	98-99	0.36391202266451117
1104	100	0.41484130330652746
1105	1	-0.06208024083669983
1105	2	-0.07801948168081552
1105	3	-0.07995336630751382
1105	4	-0.07536843645906544
1105	5	-0.0702773307942266
1105	6	0.004792714597037673
1105	7	0.04680010316447181
1105	8	0.05344769101404978
1105	9	0.10195339792378633
1105	10-11	0.12438805764166716
1105	12-13	0.1262977039844415
1105	14-15	0.15609119088372125
1105	16-17	0.0646284465799809
1105	18-19	0.10393002493969306
1105	20-21	0.08260935814389114
1105	22-23	0.043219944175660885
1105	24-25	0.04125665402833789
1105	26-27	0.06540005048131547
1105	28-29	0.11859587462656407
1105	30-31	0.10676196299288421
1105	32-33	0.10770843609476799
1105	34-35	0.10007209876971501
1105	36-37	0.10397776961237071
1105	38-39	0.14375821521122845
1105	40-41	0.17182296522071994
1105	42-43	0.1518257362447315
1105	44-45	0.10468343061977237
1105	46-47	0.024778867281519013
1105	48-49	0.04604897722227008
1105	50-51	0.02501052091841416
1105	52-53	0.1298120834125882
1105	54-55	0.06390233982983773
1105	56-57	0.10514520970144048
1105	58-59	0.08721443178571064
1105	60-61	0.05724701091133255
1105	62-63	0.07374609530462806
1105	64-65	0.04695175201634072
1105	66-67	0.0755518079881341
1105	68-69	0.15956599688258422
1105	70-71	0.08927778593513125
1105	72-73	0.07919308649892542
1105	74-75	0.06901216315355896
1105	76-77	0.08932941150904838
1105	78-79	0.0517134017826919
1105	80-81	0.11386593173330795
1105	82-83	0.07155995016457695
1105	84-85	0.03202707634624602
1105	86-87	0.0069390068953865125
1105	88-89	0.028719994684607286
1105	90-91	0.047057730762254835
1105	92-93	0.05623373407425447
1105	94-95	0.07225453185736086
1105	96-97	0.08158086169164847
1105	98-99	0.03201021439475227
1105	100	0.08130960258324293
1106	1	-0.19679466561900227
1106	2	-0.09020066219335021
1106	3	-0.07210033574181907
1106	4	-0.06317949723282368
1106	5	-0.11024513213236276
1106	6	-0.005836117025545207
1106	7	0.012796691755042389
1106	8	0.04281934431108425
1106	9	0.007429837498555969
1106	10-11	-0.0028387841133081793
1106	12-13	0.04194169540621573
1106	14-15	0.10273494105798875
1106	16-17	0.12127287564169364
1106	18-19	0.09972425657851858
1106	20-21	0.11463569170312127
1106	22-23	0.05696135352175702
1106	24-25	-0.013508529713767814
1106	26-27	0.0143007377336275
1106	28-29	-0.010555976735879824
1106	30-31	0.03979195890743625
1106	32-33	-0.010997883256308683
1106	34-35	0.05712240554198189
1106	36-37	0.005452107060648359
1106	38-39	0.04218368124927707
1106	40-41	0.06943691829746967
1106	42-43	0.04769573657205228
1106	44-45	0.07718703417859984
1106	46-47	0.0723397822763232
1106	48-49	0.11270451094297584
1106	50-51	0.08469915403914996
1106	52-53	-0.0019857317821276865
1106	54-55	-0.003177074241797584
1106	56-57	0.04736800843920008
1106	58-59	0.09052673577211579
1106	60-61	0.09749430778759915
1106	62-63	0.013434279874722677
1106	64-65	0.1284432192497178
1106	66-67	0.11249734787943311
1106	68-69	0.13693704160550624
1106	70-71	0.06622361677819555
1106	72-73	0.047794966289785634
1106	74-75	0.06748903066988987
1106	76-77	0.03773772351602389
1106	78-79	0.0439922693499355
1106	80-81	0.12002925993053282
1106	82-83	0.07396503018237865
1106	84-85	0.031521608271532386
1106	86-87	0.005491764330116666
1106	88-89	0.015843621301364408
1106	90-91	0.02947775842692124
1106	92-93	0.052962587639449055
1106	94-95	0.015063416554387032
1106	96-97	-0.0749222133048093
1106	98-99	-0.08980510639775474
1106	100	-0.05544148454590214
1107	1	-0.21956481670421013
1107	2	-0.14141299256819195
1107	3	-0.15895376147859608
1107	4	-0.10354093041578949
1107	5	-0.10437881499984769
1107	6	-0.052282093780590344
1107	7	-0.08062583785891064
1107	8	-0.0784487860298313
1107	9	-0.04844705397155025
1107	10-11	-0.03227146509135537
1107	12-13	-0.0755702210588467
1107	14-15	-0.04355974132841567
1107	16-17	-0.08565517470609052
1107	18-19	-0.015587035652018244
1107	20-21	-0.0658136363035382
1107	22-23	-0.08753534286925913
1107	24-25	-0.14367397742657317
1107	26-27	-0.14653168341783385
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2216	4	-0.13620146352846518
2216	5	-0.044710647818291704
2216	6	-0.18720623744253828
2216	7	-0.20262271700580214
2216	8	-0.07338403280169814
2216	9	-0.14343260320007545
2216	10-11	-0.18129348496257336
2216	12-13	-0.20513765883532642
2216	14-15	-0.21589899230033183
2216	16-17	-0.24914541435664717
2216	18-19	-0.24588494835294483
2216	20-21	-0.13255756567624388
2216	22-23	-0.09953718612555917
2216	24-25	-0.2102513212467798
2216	26-27	-0.23694208221555613
2216	28-29	-0.16017353085477026
2216	30-31	-0.313148737141681
2216	32-33	-0.32665396095843846
2216	34-35	-0.4746899539420468
2216	36-37	-0.4660479646752762
2216	38-39	-0.404141551554666
2216	40-41	-0.3188214208548814
2216	42-43	-0.40640167644366443
2216	44-45	-0.30934928506494686
2216	46-47	-0.2623085109849086
2216	48-49	-0.3078457055961934
2216	50-51	-0.21426522559611527
2216	52-53	-0.24887235285309828
2216	54-55	-0.36253678440480996
2216	56-57	-0.3985070764615628
2216	58-59	-0.31432112246338306
2216	60-61	-0.2675017596529159
2216	62-63	-0.30424111446421875
2216	64-65	-0.32717525372491707
2216	66-67	-0.34288703944210397
2216	68-69	-0.3069247278356215
2216	70-71	-0.33955517903591925
2216	72-73	-0.338756594354102
2216	74-75	-0.34620654988041366
2216	76-77	-0.22068333894669934
2216	78-79	-0.26157445837506543
2216	80-81	-0.1344317210910404
2216	82-83	-0.3299174731272174
2216	84-85	-0.2037426601233676
2216	86-87	-0.19026363484156406
2216	88-89	-0.2669201632237872
2216	90-91	-0.2800169782227435
2216	92-93	-0.4365813546888404
2216	94-95	-0.33725339552491107
2216	96-97	-0.2899303625854799
2216	98-99	-0.19963932685874397
2216	100	-0.2168388924347866
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	36987.0
3	7372.0
4	4435.0
5	3793.0
6	3459.0
7	3838.0
8	4505.0
9	5186.0
10	5626.0
11	6232.0
12	6988.0
13	7706.0
14	8689.0
15	8875.0
16	8680.0
17	8934.0
18	8652.0
19	8663.0
20	8609.0
21	9112.0
22	10678.0
23	14799.0
24	21977.0
25	27863.0
26	28423.0
27	27075.0
28	31038.0
29	39362.0
30	48351.0
31	60610.0
32	80171.0
33	109491.0
34	160914.0
35	253875.0
36	474512.0
37	948280.0
38	1348719.0
39	456123.0
40	399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52583981878282	19.1050369969592	13.061290835691238	24.307832348566738
2	15.872079835644515	19.940964638914537	39.737289392835194	24.449666132605756
3	18.64069067252614	24.753340362783728	29.545913139305952	27.060055825384175
4	12.612007527435356	16.345208575591016	38.15691167990331	32.885872217070315
5	13.44214271545591	37.73025871107616	35.28943391651062	13.538164656957315
6	31.917339381671262	37.20406056235142	17.559295893564116	13.319304162413204
7	28.206550445341648	32.046272049324145	21.948237889043895	17.798939616290312
8	25.73386493721178	35.74420477667596	20.171839722499243	18.350090563613012
9	25.620448655244516	16.6236731136477	20.401104878175126	37.35477335293266
10-11	24.50341725680713	26.335335449394737	28.514479130986153	20.64676816281198
12-13	25.106808070717513	23.795223547723868	28.281011511342914	22.81695687021571
14-15	22.431571631799816	25.582290313051864	25.317784760479654	26.668353294668663
16-17	22.347517345071825	28.9009864291099	25.82056112706621	22.930935098752066
18-19	22.28888941800386	28.018046950178082	27.56906837759219	22.12399525422587
20-21	23.51262680021374	27.323889059808277	26.953228210362546	22.210255929615435
22-23	23.15752138002457	27.039432590518253	26.995941104214705	22.807104925242477
24-25	22.957998724023035	27.164057049656677	26.97816180627457	22.89978242004572
26-27	22.709245829346628	27.110743571469342	27.090575130696728	23.089435468487306
28-29	22.718722136757368	27.194733811583028	27.159737665919643	22.926806385739965
30-31	22.975382104931406	27.581694822826265	26.769118717120534	22.673804355121792
32-33	22.663194389207128	27.24326568879487	27.224953671292436	22.86858625070557
34-35	22.807830369765398	27.01799286858884	27.228653031882676	22.94552372976309
36-37	23.133520455827515	27.308263005669286	26.70560040512635	22.852616133376845
38-39	22.93543475107887	27.548140684471136	26.869171440372806	22.64725312407719
40-41	22.70963850482697	27.19842779613329	27.18724455200075	22.90468914703899
42-43	22.72810986060727	27.110573595737865	27.11294248250043	23.048374061154437
44-45	22.790879036137753	27.20860423418746	27.12267615343643	22.877840576238352
46-47	22.876783579830278	27.36259477848952	26.962119261715735	22.79850237996447
48-49	22.842642128564687	27.235432911494712	26.97255453611512	22.94937042382548
50-51	22.810827004459483	27.172806269402468	26.963621046715712	23.05274567942234
52-53	22.852710974675396	27.086475887364358	27.04549640218932	23.01531673577092
54-55	22.8699675491303	27.48389416009746	26.842842021809478	22.803296268962768
56-57	22.75690076305387	27.188038042617503	26.948738980213733	23.106322214114893
58-59	22.6613543522267	26.858514693045247	27.232705892242876	23.24742506248518
60-61	22.91036589670329	27.227651153458893	26.908529539619614	22.95345341021821
62-63	22.734300831752627	27.27933103085678	26.868912107694964	23.11745602969563
64-65	22.748700376151294	27.018324080881584	27.006817538988713	23.226158003978405
66-67	22.601182070483468	27.125175881229108	26.912667001534775	23.36097504675265
68-69	22.918184828644506	27.143551823257383	26.872001122104578	23.066262225993533
70-71	22.907982605832437	26.904405688051625	27.00575246586498	23.18185924025096
72-73	22.7413786237401	27.112663424085437	27.021999171726502	23.12395878044796
74-75	22.760029954893504	27.161611294620307	26.897880517128275	23.18047823335791
76-77	22.775000252514026	27.0703044320681	26.867852039088476	23.286843276329407
78-79	22.854901237811383	27.211812478094416	26.90108549537125	23.03220078872295
80-81	22.837610492901895	26.93780496412405	27.050863418792943	23.173721124181114
82-83	22.704128603858962	27.00980099642264	27.16448635845535	23.121584041263052
84-85	22.700401349455415	26.942581744740636	26.937659775367816	23.419357130436136
86-87	22.80785150061384	27.058491808333006	26.934945850118773	23.198710840934375
88-89	22.6296870182503	26.973455707816836	26.990392401959873	23.406464871972986
90-91	22.63961711282164	26.827767747830844	27.09939467822482	23.4332204611227
92-93	22.780735180117524	26.98015750576177	26.974678398456938	23.26442891566377
94-95	22.577604945078864	26.689774656470828	27.162134161382532	23.57048623706778
96-97	22.620468693317854	26.785576434738445	27.138799970978745	23.455154900964956
98-99	22.54800078659556	26.893267944675493	27.05577607936907	23.502955189359877
100	22.694920519784954	26.701971599713758	26.97843764526251	23.624670235238774
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	419.0
1	411.5
2	412.0
3	399.5
4	398.0
5	426.5
6	425.5
7	399.0
8	377.5
9	352.5
10	315.5
11	270.5
12	271.5
13	289.0
14	314.0
15	366.0
16	416.5
17	474.0
18	609.0
19	807.5
20	1124.5
21	1682.5
22	2458.5
23	3743.0
24	5725.0
25	8445.5
26	12346.0
27	17537.5
28	24522.0
29	33851.5
30	44126.0
31	55551.5
32	67980.0
33	81442.0
34	94607.5
35	106893.0
36	118728.0
37	129944.5
38	140362.0
39	147509.5
40	153089.0
41	157854.0
42	159751.0
43	161230.0
44	161094.5
45	159501.0
46	159140.0
47	156189.0
48	152951.0
49	150036.0
50	146025.5
51	142295.0
52	135448.0
53	129849.5
54	125556.0
55	119859.5
56	112378.0
57	103068.5
58	93898.5
59	85485.5
60	77064.5
61	66859.5
62	57288.5
63	48436.5
64	40123.0
65	32989.0
66	26774.0
67	21118.0
68	16705.5
69	13277.0
70	10031.0
71	7323.0
72	5486.0
73	4120.0
74	2998.5
75	2187.5
76	1540.0
77	1056.0
78	745.5
79	522.5
80	401.5
81	251.5
82	126.5
83	78.5
84	65.0
85	44.0
86	31.0
87	23.5
88	17.5
89	15.0
90	13.5
91	8.0
92	6.0
93	7.5
94	6.0
95	4.0
96	2.5
97	2.0
98	2.0
99	1.5
100	12.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0595497657113563
2	0.04065907619886837
3	0.05472266077450435
4	0.07314920558152574
5	0.0866790237458752
6	0.08554186921748219
7	0.08742165527462166
8	0.09960545379311816
9	0.09496400673845284
10-11	0.09991875146930808
12-13	0.10561612772890978
14-15	0.10569735305236644
16-17	0.07349731411062564
18-19	0.095578998473196
20-21	0.0894058738904911
22-23	0.055952644243990664
24-25	0.021571125186557162
26-27	0.17931070333935872
28-29	0.06527034920623133
30-31	0.07686236322525801
32-33	0.07160592443584951
34-35	0.020364348952344173
36-37	0.05097469227786208
38-39	0.07448362160974202
40-41	0.08012297978116041
42-43	0.07382221540445222
44-45	0.07135064484784293
46-47	0.07819677925347429
48-49	0.07443720713919537
50-51	0.06981896731980336
52-53	0.0739846660513655
54-55	0.06495705153004142
56-57	0.06690645929300086
58-59	0.06451611405984821
60-61	0.06147596623904242
62-63	0.06760267635120065
64-65	0.06393593317801505
66-67	0.0656300613529679
68-69	0.06514270941222802
70-71	0.05801808818331674
72-73	0.05691574450783372
74-75	0.058621476300423234
76-77	0.053597109863748
78-79	0.05025526798438896
80-81	0.04495241472443381
82-83	0.05091667418967876
84-85	0.04135529325706817
86-87	0.0393826782588354
88-89	0.04142491496288815
90-91	0.037653739230972566
92-93	0.040148517022855185
94-95	0.039185416759012125
96-97	0.04208632116817796
98-99	0.042515655020734504
100	0.04973310519073911
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4309001.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.85768502205088
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.46254575457046	37.24487049346934
2	15.129517278500481	15.9942251768531
3	5.8455317779947125	9.26943832522901
4	2.8928324665737715	6.116337093588759
5	1.616993858978959	4.273527604025019
6	0.9957764689288168	3.1580663368205664
7	0.6448766717313806	2.3860681594712077
8	0.4478529815625125	1.893797746849412
9	0.3237173139601152	1.5399853035739313
>10	1.5483259223825727	13.971268278338588
>50	0.07163958913979086	2.5379991743533488
>100	0.0202580789557184	1.572349297622246
>500	1.318367205715373E-4	0.0420670098055114
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.3207235273326693E-5	0.0
10-11	0.0	0.0	0.0	4.6414470546653386E-5	0.0
12-13	0.0	0.0	0.0	4.6414470546653386E-5	0.0
14-15	0.0	1.1603617636663346E-5	0.0	4.6414470546653386E-5	0.0
16-17	0.0	2.3207235273326693E-5	0.0	4.6414470546653386E-5	0.0
18-19	0.0	5.8018088183316734E-5	0.0	4.6414470546653386E-5	0.0
20-21	0.0	9.282894109330677E-5	0.0	4.6414470546653386E-5	0.0
22-23	0.0	9.282894109330677E-5	0.0	4.6414470546653386E-5	0.0
24-25	0.0	1.2763979400329682E-4	0.0	1.2763979400329682E-4	0.0
26-27	0.0	1.3924341163996018E-4	0.0	2.2046873509660357E-4	0.0
28-29	0.0	1.508470292766235E-4	0.0	9.514966462063945E-4	0.0
30-31	0.0	1.972614998232769E-4	0.0	0.0037247612613689344	0.0
32-33	0.0	2.3207235273326694E-4	0.0	0.01119749101938013	0.0
34-35	0.0	2.3207235273326694E-4	0.0	0.022545829068036885	0.0
36-37	0.0	2.6688320564325697E-4	0.0	0.04349035890221423	0.0
38-39	0.0	3.01694058553247E-4	0.0	0.07768622007746111	0.0
40-41	0.0	3.249012938265737E-4	0.0	0.11713852004211649	0.0
42-43	0.0	3.481085290999004E-4	0.0	0.15922484121029445	0.0
44-45	0.0	3.481085290999004E-4	0.0	0.2118240399572894	0.0
46-47	0.0	3.7131576437322714E-4	0.0	0.276050063576221	0.0
48-49	0.0	3.945229996465538E-4	0.0	0.34474347998526805	0.0
50-51	0.0	4.0612661728321714E-4	0.0	0.4216290504457994	0.0
52-53	0.0	4.873519407398606E-4	0.0	0.49858424261215073	0.0
54-55	0.0	4.873519407398606E-4	0.0	0.5863424027982356	0.0
56-57	0.0	4.873519407398606E-4	0.0	0.6925967294971619	0.0
58-59	0.0	4.873519407398606E-4	0.0	0.8130306769480907	0.0
60-61	0.0	4.873519407398606E-4	0.0	0.9406936781866608	0.0
62-63	0.0	4.873519407398606E-4	0.0	1.0689136530717909	0.0
64-65	0.0	4.873519407398606E-4	0.0	1.21139447403238	0.0
66-67	0.0	4.873519407398606E-4	0.0	1.3717100553005208	0.0
68-69	0.0	4.873519407398606E-4	0.0	1.5587719752211708	0.0
70-71	0.0	4.873519407398606E-4	0.0	1.761023030628213	0.0
72-73	0.0	4.873519407398606E-4	0.0	1.9709208700578162	0.0
74-75	0.0	4.98955558376524E-4	0.0	2.184288191160782	0.0
76-77	0.0	5.105591760131873E-4	0.0	2.421721415242187	0.0
78-79	0.0	5.105591760131873E-4	0.0	2.693234464322473	0.0
80-81	0.0	5.221627936498505E-4	0.0	2.992723835524754	0.0
82-83	0.0	5.453700289231773E-4	0.0	3.2972375731637102	0.0
84-85	0.0	5.569736465598407E-4	0.0	3.608585841590661	0.0
86-87	0.0	5.569736465598407E-4	0.0	3.9522037706651725	0.0
88	0.0	5.569736465598407E-4	0.0	4.229054483858324	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5510	0.0	36.094696	1
GGTATCA	3410	0.0	34.883556	1
TCAACGC	5670	0.0	32.916344	4
ATCAACG	5930	0.0	31.86952	3
CAACGCA	6030	0.0	31.107836	5
AACGCAG	6280	0.0	29.488312	6
GTACATG	13595	0.0	28.808483	1
TATCAAC	6910	0.0	28.234116	2
TACATGG	13705	0.0	27.819284	2
ACATGGG	14430	0.0	25.607098	3
ACGCAGA	7495	0.0	24.206612	7
CGCAGAG	7985	0.0	22.44772	8
CATGGGG	10590	0.0	21.929823	4
TACACCG	655	0.0	20.096987	5
GAGTACA	9630	0.0	19.871138	1
AGTACAT	9770	0.0	18.092451	2
GCAGAGT	9665	0.0	18.062803	9
ACCGTCC	440	5.456968E-12	17.107615	8
GTGTAAG	1940	0.0	15.995462	1
AGAGTAC	8065	0.0	15.876027	10-11
>>END_MODULE