FastQCFastQC Report
Wed 25 May 2016
SRR1033010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033010_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4309001
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52410.1216291200675052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44380.10299371014302386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52050.049.433221
GTATCAA94950.044.1218571
TCAACGC112950.036.5749664
ATCAACG113700.036.2510343
CAACGCA114650.036.0326425
AACGCAG120600.034.332856
TATCAAC124200.033.5263062
ACGCAGA143150.028.8588377
GTACATG128750.027.8171941
CGCAGAG147950.027.7637258
TACATGG131050.026.1667252
GCAGAGT165550.024.6133799
TACACCG5800.024.3094185
ACATGGG136100.024.2070123
ACACCGT8300.020.951016
CATGGGG99250.020.5987114
GAGTACT98800.020.5732512-13
AGAGTAC146300.020.5595910-11
GAGTACA93950.020.3144571
CAGAGTA156100.020.09681110-11
Produced by FastQC (version 0.11.5)