##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033010_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4309001 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6533688434976 34.0 31.0 34.0 31.0 34.0 2 32.66090167999497 34.0 31.0 34.0 31.0 34.0 3 32.764782834814845 34.0 31.0 34.0 31.0 34.0 4 36.267784110516565 37.0 37.0 37.0 35.0 37.0 5 36.24980384084385 37.0 37.0 37.0 35.0 37.0 6 36.31626263256843 37.0 37.0 37.0 35.0 37.0 7 36.26405053050579 37.0 37.0 37.0 35.0 37.0 8 36.272479862501775 37.0 37.0 37.0 35.0 37.0 9 38.06690901208888 39.0 39.0 39.0 35.0 39.0 10-11 38.02317613757806 39.0 38.5 39.0 35.0 39.0 12-13 37.99900127663001 39.0 38.0 39.0 35.0 39.0 14-15 39.477436649469325 41.0 39.0 41.0 36.0 41.0 16-17 39.468414140539764 41.0 39.0 41.0 36.0 41.0 18-19 39.478064753292 41.0 39.0 41.0 36.5 41.0 20-21 39.4644090358763 41.0 39.0 41.0 36.0 41.0 22-23 39.39177607524343 41.0 39.0 41.0 36.0 41.0 24-25 39.33530823037637 41.0 39.0 41.0 36.0 41.0 26-27 39.21854764016068 41.0 39.0 41.0 36.0 41.0 28-29 39.17049067753756 41.0 39.0 41.0 36.0 41.0 30-31 39.178091395198095 41.0 39.0 41.0 36.0 41.0 32-33 39.14950901612694 41.0 39.0 41.0 35.0 41.0 34-35 39.07217879503857 41.0 39.0 41.0 35.0 41.0 36-37 38.98718392035648 41.0 39.0 41.0 35.0 41.0 38-39 38.895327478457304 41.0 39.0 41.0 35.0 41.0 40-41 38.79298299072105 41.0 39.0 41.0 35.0 41.0 42-43 38.67476162108108 41.0 38.0 41.0 35.0 41.0 44-45 38.511246342249635 40.0 38.0 41.0 35.0 41.0 46-47 38.326092869321684 40.0 38.0 41.0 34.0 41.0 48-49 38.13036710829262 40.0 38.0 41.0 34.0 41.0 50-51 37.982830939236266 40.0 38.0 41.0 34.0 41.0 52-53 37.837050745636866 40.0 37.0 41.0 33.0 41.0 54-55 37.66825628492544 40.0 37.0 41.0 33.0 41.0 56-57 37.49583464937697 40.0 36.5 41.0 33.0 41.0 58-59 37.29512815151354 40.0 36.0 41.0 33.0 41.0 60-61 37.0796421258663 39.0 35.5 41.0 33.0 41.0 62-63 36.82924847313797 39.0 35.0 41.0 32.0 41.0 64-65 36.54202923136941 39.0 35.0 41.0 32.0 41.0 66-67 36.24556968076823 38.0 35.0 40.5 31.5 41.0 68-69 35.92063879771669 37.0 35.0 40.0 31.0 41.0 70-71 35.5836691613671 37.0 35.0 39.5 31.0 41.0 72-73 35.23305982059415 36.0 35.0 39.0 31.0 41.0 74-75 34.88584581437786 36.0 35.0 39.0 31.0 40.5 76-77 34.539739257428806 35.0 34.5 37.5 30.0 39.5 78-79 34.19119362005254 35.0 34.0 37.0 30.0 39.0 80-81 33.87870599240984 35.0 34.0 37.0 30.0 39.0 82-83 33.60589832770984 35.0 34.0 36.0 30.0 38.0 84-85 33.4126415844415 35.0 34.0 36.0 30.0 37.0 86-87 33.23125209300253 35.0 34.0 35.5 30.0 37.0 88-89 33.04500323856968 35.0 34.0 35.0 29.5 36.5 90-91 32.880416249613305 35.0 34.0 35.0 29.0 36.0 92-93 32.7202664840412 35.0 34.0 35.0 29.0 36.0 94-95 32.575017039912495 35.0 34.0 35.0 29.0 36.0 96-97 32.43634649887526 35.0 34.0 35.0 29.0 35.5 98-99 32.31257732360703 35.0 34.0 35.0 29.0 35.0 100 32.2081185407012 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1659118865875584 1101 2 0.19371406302367689 1101 3 0.19362939400905788 1101 4 0.14324378213255784 1101 5 0.14211512761175982 1101 6 0.1600003548919844 1101 7 0.18884265173777237 1101 8 0.18993202112972796 1101 9 0.2618133790752424 1101 10-11 0.28453014342083094 1101 12-13 0.2959111365504157 1101 14-15 0.3829097517898674 1101 16-17 0.3863746729334565 1101 18-19 0.39192508912975654 1101 20-21 0.32415637475737924 1101 22-23 0.3831851422194461 1101 24-25 0.4375988635799928 1101 26-27 0.4757356155843411 1101 28-29 0.47571071500740203 1101 30-31 0.4447529671325725 1101 32-33 0.4306100424139174 1101 34-35 0.42787333205801303 1101 36-37 0.4154410231087624 1101 38-39 0.4352224994519176 1101 40-41 0.45192218369214743 1101 42-43 0.4583259836973639 1101 44-45 0.419980813545358 1101 46-47 0.4427822848616998 1101 48-49 0.4250167294627687 1101 50-51 0.44276844580892316 1101 52-53 0.4583239699713886 1101 54-55 0.5080016119300339 1101 56-57 0.4349916864284964 1101 58-59 0.45778607763813994 1101 60-61 0.4273165313695415 1101 62-63 0.40532369971788285 1101 64-65 0.3959488762735077 1101 66-67 0.4278468622178764 1101 68-69 0.425734256899851 1101 70-71 0.3833483452545039 1101 72-73 0.4062678328550504 1101 74-75 0.38083978277125397 1101 76-77 0.4284319679361843 1101 78-79 0.4234936591636611 1101 80-81 0.44245044789622767 1101 82-83 0.4358638126722383 1101 84-85 0.4427179209828651 1101 86-87 0.4816146024797163 1101 88-89 0.5110141873291454 1101 90-91 0.5294613803591091 1101 92-93 0.5100881424370556 1101 94-95 0.49863708614144286 1101 96-97 0.5506333070643521 1101 98-99 0.5249930049371656 1101 100 0.49189722593740015 1102 1 0.10952226156675238 1102 2 0.12979092799788106 1102 3 0.15732080751035937 1102 4 0.07623465409681529 1102 5 0.14854828043274182 1102 6 0.1424759345158506 1102 7 0.15678058621562485 1102 8 0.15415088915087694 1102 9 0.17439867436015533 1102 10-11 0.2079090529214156 1102 12-13 0.23560689002943747 1102 14-15 0.30448353781231674 1102 16-17 0.3057515852761483 1102 18-19 0.26089872920606183 1102 20-21 0.3210172522266177 1102 22-23 0.3277179224936688 1102 24-25 0.3094264550843846 1102 26-27 0.3110581053745136 1102 28-29 0.32464684436178004 1102 30-31 0.31040057118322295 1102 32-33 0.31835088557300395 1102 34-35 0.3119594402886108 1102 36-37 0.3222854496299874 1102 38-39 0.3440163953180573 1102 40-41 0.32634704062651565 1102 42-43 0.3002595781974904 1102 44-45 0.3214015481409973 1102 46-47 0.31998363934442153 1102 48-49 0.35898633681640035 1102 50-51 0.3757753206408836 1102 52-53 0.3620977662356779 1102 54-55 0.32577183635425655 1102 56-57 0.33563404153853327 1102 58-59 0.31938015529685515 1102 60-61 0.3467616159286848 1102 62-63 0.3438531641979239 1102 64-65 0.3411944242757059 1102 66-67 0.29220943599109717 1102 68-69 0.37119712384560444 1102 70-71 0.3150148198867555 1102 72-73 0.33531335781312066 1102 74-75 0.37744717481591294 1102 76-77 0.33945442316016283 1102 78-79 0.39314390048690484 1102 80-81 0.354125500065571 1102 82-83 0.3285972305007121 1102 84-85 0.40995396968278897 1102 86-87 0.4157131929471305 1102 88-89 0.47881417708731533 1102 90-91 0.39575165957715086 1102 92-93 0.44134174308229035 1102 94-95 0.4616765428120857 1102 96-97 0.4919779960326096 1102 98-99 0.4637625124329148 1102 100 0.4940908990436199 1103 1 0.1661768404056616 1103 2 0.03586895442193594 1103 3 0.11025905148171233 1103 4 0.04883709955735327 1103 5 0.09790711583383427 1103 6 0.1295265829622707 1103 7 0.15509613229360752 1103 8 0.14909943186185615 1103 9 0.16461630007104588 1103 10-11 0.20214753377609185 1103 12-13 0.2020560122055599 1103 14-15 0.29878461434597625 1103 16-17 0.3045152716042949 1103 18-19 0.3230419268342004 1103 20-21 0.35571438716497994 1103 22-23 0.26771454484763524 1103 24-25 0.24296551394785126 1103 26-27 0.25466310955595617 1103 28-29 0.28383320136047985 1103 30-31 0.2483841052988538 1103 32-33 0.23529564789707536 1103 34-35 0.28741285986114207 1103 36-37 0.311028632022321 1103 38-39 0.2959502797174167 1103 40-41 0.2472701294418016 1103 42-43 0.24762001503971476 1103 44-45 0.31305272692825525 1103 46-47 0.31459101459977745 1103 48-49 0.2761985241250784 1103 50-51 0.2527976651977468 1103 52-53 0.27983375474607897 1103 54-55 0.2828471925894931 1103 56-57 0.33500779067522046 1103 58-59 0.2875121910091565 1103 60-61 0.2801646167123266 1103 62-63 0.273881375664331 1103 64-65 0.28651981537967686 1103 66-67 0.27316462364649396 1103 68-69 0.25547348138763937 1103 70-71 0.32568322410864425 1103 72-73 0.3658131514014258 1103 74-75 0.31097952599923673 1103 76-77 0.3004933741966198 1103 78-79 0.2648597558743617 1103 80-81 0.2188411420395724 1103 82-83 0.27394876615336017 1103 84-85 0.314608609576986 1103 86-87 0.2725058323674503 1103 88-89 0.2586626395301863 1103 90-91 0.27502358360867873 1103 92-93 0.34486608986067324 1103 94-95 0.30726038265228794 1103 96-97 0.2851672640804779 1103 98-99 0.3714335638778792 1103 100 0.39848281913176464 1104 1 0.12016512570157545 1104 2 0.15383867086972458 1104 3 0.17040623927371712 1104 4 0.10733628816255703 1104 5 0.07743106638878317 1104 6 0.09205818985577707 1104 7 0.13483332408645765 1104 8 0.11941645149293834 1104 9 0.12884648744854132 1104 10-11 0.19362952984702275 1104 12-13 0.17623594873953863 1104 14-15 0.22603257810425248 1104 16-17 0.2353436184332267 1104 18-19 0.2713031906905883 1104 20-21 0.2415027908459848 1104 22-23 0.29701057288912835 1104 24-25 0.27268872698316926 1104 26-27 0.2791834741617265 1104 28-29 0.2951481051423954 1104 30-31 0.30326344823483 1104 32-33 0.32813667317672923 1104 34-35 0.2868918076523528 1104 36-37 0.29339414636399397 1104 38-39 0.2623371974052944 1104 40-41 0.23788962252803714 1104 42-43 0.24668981363617348 1104 44-45 0.2464368478108625 1104 46-47 0.28003353191420643 1104 48-49 0.18828552763093853 1104 50-51 0.2908772084486344 1104 52-53 0.30802989626315025 1104 54-55 0.27872393872166157 1104 56-57 0.3277982941015196 1104 58-59 0.3198281548637425 1104 60-61 0.3277159401792602 1104 62-63 0.34155921884322993 1104 64-65 0.31494501829998 1104 66-67 0.31182685155215495 1104 68-69 0.37978596280638044 1104 70-71 0.32592471467678763 1104 72-73 0.35514429399536596 1104 74-75 0.33135389947137384 1104 76-77 0.32260703366781485 1104 78-79 0.2604810421378332 1104 80-81 0.32982210118039745 1104 82-83 0.2561816667094732 1104 84-85 0.2876856063351667 1104 86-87 0.3510419966574503 1104 88-89 0.33958914839094945 1104 90-91 0.32463912116722327 1104 92-93 0.26908941633914196 1104 94-95 0.3061775186797959 1104 96-97 0.3524157201417708 1104 98-99 0.3259949417556456 1104 100 0.29755532512458416 1105 1 0.058522227128477766 1105 2 0.06913453453267238 1105 3 0.11845907187443316 1105 4 0.048989874806402156 1105 5 0.049434403663475734 1105 6 0.08255638761754369 1105 7 0.08881374232431227 1105 8 0.09949682159358986 1105 9 0.13350203421096296 1105 10-11 0.14614891283711273 1105 12-13 0.16342274858880756 1105 14-15 0.21296674385649794 1105 16-17 0.18407282547779857 1105 18-19 0.16926908368184002 1105 20-21 0.19935915539149107 1105 22-23 0.18810106151567396 1105 24-25 0.21838881670213084 1105 26-27 0.19726286209460397 1105 28-29 0.20495782195835233 1105 30-31 0.1713903536128214 1105 32-33 0.1843725631407125 1105 34-35 0.1782629156400759 1105 36-37 0.20668087251873146 1105 38-39 0.21451749866731262 1105 40-41 0.2086865649015266 1105 42-43 0.17614013832273656 1105 44-45 0.16279032444322894 1105 46-47 0.15129922107306726 1105 48-49 0.16637082952740911 1105 50-51 0.16719661461922897 1105 52-53 0.1568453337015825 1105 54-55 0.1519233329620846 1105 56-57 0.14175604044181256 1105 58-59 0.14474732025882986 1105 60-61 0.19516745507016964 1105 62-63 0.18282397643781678 1105 64-65 0.15609593081765638 1105 66-67 0.17590748387088695 1105 68-69 0.17935051720749584 1105 70-71 0.11902303738828834 1105 72-73 0.09528436343715896 1105 74-75 0.10050571722928936 1105 76-77 0.11708683169343459 1105 78-79 0.12525427779790732 1105 80-81 0.1337054591105158 1105 82-83 0.16301695886221523 1105 84-85 0.17088924692412633 1105 86-87 0.1477461503734787 1105 88-89 0.11761955316649164 1105 90-91 0.16384923644734073 1105 92-93 0.1916552638128053 1105 94-95 0.14534439097639051 1105 96-97 0.13443250439978982 1105 98-99 0.10609900349087553 1105 100 0.09644554966735086 1106 1 0.021612086368442363 1106 2 0.04089633232361933 1106 3 0.07301446727181826 1106 4 0.0391034551012055 1106 5 0.06861333778594059 1106 6 0.06279494381791295 1106 7 0.10483742579788924 1106 8 0.11255413060496977 1106 9 0.06551606293517409 1106 10-11 0.11538779514591369 1106 12-13 0.08587082885269837 1106 14-15 0.17214033174030163 1106 16-17 0.17477800769240304 1106 18-19 0.15252402167881485 1106 20-21 0.16450010333659293 1106 22-23 0.1646465306087208 1106 24-25 0.10868599968291193 1106 26-27 0.11272876679093713 1106 28-29 0.09798188973072541 1106 30-31 0.11412995542107751 1106 32-33 0.0816947764592797 1106 34-35 0.083257163281246 1106 36-37 0.08498732716238067 1106 38-39 0.13009469241330152 1106 40-41 0.1692934539119051 1106 42-43 0.11520992916479145 1106 44-45 0.1427092580080327 1106 46-47 0.16003813914468168 1106 48-49 0.17012883524704137 1106 50-51 0.1624128827021778 1106 52-53 0.13124545784099695 1106 54-55 0.19431603540693487 1106 56-57 0.13515895165667047 1106 58-59 0.14104168585669186 1106 60-61 0.19732801437643843 1106 62-63 0.15593300212521655 1106 64-65 0.17537378853981522 1106 66-67 0.13819328578850332 1106 68-69 0.1471316099495823 1106 70-71 0.15418619465135208 1106 72-73 0.11466478165607441 1106 74-75 0.12606613275690393 1106 76-77 0.09898332802804788 1106 78-79 0.14770932878114706 1106 80-81 0.14822183060257998 1106 82-83 0.16227297095610993 1106 84-85 0.1595730417594936 1106 86-87 0.15218383153641213 1106 88-89 0.10975779404788 1106 90-91 0.15192354795242835 1106 92-93 0.16681683296415883 1106 94-95 0.1344623100893756 1106 96-97 0.08709156106143467 1106 98-99 0.16010874310116918 1106 100 0.17787506842100953 1107 1 -0.027110278006965416 1107 2 0.022183938037947826 1107 3 0.03438861987228847 1107 4 0.03270051938621066 1107 5 0.007710033712143627 1107 6 0.032543274583190396 1107 7 0.028200669400419542 1107 8 0.022958116781637727 1107 9 0.040051431189382924 1107 10-11 0.033517322647334424 1107 12-13 0.06628204276341165 1107 14-15 0.0944091895026773 1107 16-17 0.055709688175433314 1107 18-19 0.10286972117705062 1107 20-21 0.09190181428765243 1107 22-23 0.10242654720229183 1107 24-25 0.0710483299415543 1107 26-27 0.10970102019389572 1107 28-29 0.13593620859231947 1107 30-31 0.03591971434942565 1107 32-33 0.06844420184280153 1107 34-35 0.05675124978629498 1107 36-37 0.08484962837064813 1107 38-39 0.11563910811724298 1107 40-41 0.08613267982391903 1107 42-43 0.1284247137155674 1107 44-45 0.14887804767631962 1107 46-47 0.10135428395036428 1107 48-49 0.09904453569813398 1107 50-51 0.11071064675268616 1107 52-53 0.11613321880054173 1107 54-55 0.10554086906446258 1107 56-57 0.11528919031133 1107 58-59 0.1013183151761865 1107 60-61 0.11281848933850114 1107 62-63 0.08715762662002646 1107 64-65 0.05278735204510099 1107 66-67 0.07344905693659598 1107 68-69 0.09717332629239195 1107 70-71 0.0958343271768527 1107 72-73 0.07058652690982115 1107 74-75 0.11870743960559338 1107 76-77 0.12426215355612413 1107 78-79 0.1505535931907147 1107 80-81 0.17136882319125135 1107 82-83 0.13742304520888382 1107 84-85 0.11048301580006381 1107 86-87 0.11200529953335803 1107 88-89 0.13835719398327484 1107 90-91 0.09323097764470134 1107 92-93 0.18046830279708104 1107 94-95 0.16245316270634902 1107 96-97 0.19672036695145323 1107 98-99 0.2280825605764889 1107 100 0.23373545927641715 1108 1 -0.028364978624090043 1108 2 -0.0107235890966777 1108 3 0.003035982493109657 1108 4 0.02056355046351399 1108 5 0.013644905275384644 1108 6 0.027660423628276476 1108 7 -0.03175091903176508 1108 8 -0.015302256203035824 1108 9 -0.01894537849364042 1108 10-11 -0.050505323168685834 1108 12-13 -0.03388105896235061 1108 14-15 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74-75 -0.3120978993902739 2214 76-77 -0.27876953260838633 2214 78-79 -0.29845480555889026 2214 80-81 -0.27348913218639126 2214 82-83 -0.28772292707984803 2214 84-85 -0.18699921255576868 2214 86-87 -0.19964220406701116 2214 88-89 -0.19834194617463652 2214 90-91 -0.19016037395676477 2214 92-93 -0.21399401641518523 2214 94-95 -0.23421148931257108 2214 96-97 -0.24290602051014076 2214 98-99 -0.315598978016574 2214 100 -0.29334929761179396 2215 1 -0.21793122176908497 2215 2 -0.16553422615080393 2215 3 -0.21734592009754294 2215 4 -0.15083751121064637 2215 5 -0.17548324359877654 2215 6 -0.18648279738405904 2215 7 -0.20933434460518185 2215 8 -0.19955858220564693 2215 9 -0.2608104520254386 2215 10-11 -0.2298957348833781 2215 12-13 -0.2197231285358754 2215 14-15 -0.3608267510273748 2215 16-17 -0.34664971712515324 2215 18-19 -0.2899443275193505 2215 20-21 -0.31643690581577033 2215 22-23 -0.3578190895139315 2215 24-25 -0.4047866194228078 2215 26-27 -0.3953625436932029 2215 28-29 -0.39072830336630915 2215 30-31 -0.4197256207076805 2215 32-33 -0.3951843264916093 2215 34-35 -0.4040977051802983 2215 36-37 -0.392396654757988 2215 38-39 -0.3996485454057108 2215 40-41 -0.3745546720399062 2215 42-43 -0.39447983271838893 2215 44-45 -0.4092559751635818 2215 46-47 -0.4250732101241894 2215 48-49 -0.4013109911279855 2215 50-51 -0.39000040689954574 2215 52-53 -0.32314710871507657 2215 54-55 -0.3689366142365458 2215 56-57 -0.33745806243592114 2215 58-59 -0.3232561299864898 2215 60-61 -0.3267958179228643 2215 62-63 -0.3280438385814364 2215 64-65 -0.33140786450304915 2215 66-67 -0.349724828001996 2215 68-69 -0.3846193907944411 2215 70-71 -0.3617610186537874 2215 72-73 -0.32346647890788915 2215 74-75 -0.3265414291726927 2215 76-77 -0.3069056981999623 2215 78-79 -0.36673793880670047 2215 80-81 -0.3363234845010581 2215 82-83 -0.35559570075104574 2215 84-85 -0.35530022057140087 2215 86-87 -0.31668463798011004 2215 88-89 -0.27268568470666565 2215 90-91 -0.29082418288104606 2215 92-93 -0.3019600531606841 2215 94-95 -0.2666353957939762 2215 96-97 -0.23695771966780654 2215 98-99 -0.24671812893008394 2215 100 -0.24932853555228363 2216 1 -0.26766257797299886 2216 2 -0.2586208312418421 2216 3 -0.25819980138141574 2216 4 -0.18624627244137315 2216 5 -0.21710203234163572 2216 6 -0.22411565414176948 2216 7 -0.22227903241066826 2216 8 -0.22698607589116904 2216 9 -0.2834777860569275 2216 10-11 -0.32262646301519027 2216 12-13 -0.363998635607814 2216 14-15 -0.474615117904861 2216 16-17 -0.4202684378421466 2216 18-19 -0.44645800380195055 2216 20-21 -0.47930668180895 2216 22-23 -0.47825240758034937 2216 24-25 -0.4856189825261765 2216 26-27 -0.4776959774536067 2216 28-29 -0.5124759961141692 2216 30-31 -0.4981502017859185 2216 32-33 -0.4821082887512702 2216 34-35 -0.5003623851361354 2216 36-37 -0.48622196568035747 2216 38-39 -0.4581606064075672 2216 40-41 -0.5061274332846395 2216 42-43 -0.5064451398626559 2216 44-45 -0.5769447165000869 2216 46-47 -0.4723778354325816 2216 48-49 -0.5419471229641033 2216 50-51 -0.5400349617027942 2216 52-53 -0.5626225909775826 2216 54-55 -0.5223208174644753 2216 56-57 -0.5865689660977935 2216 58-59 -0.5599058046378005 2216 60-61 -0.5892216584799144 2216 62-63 -0.5853485989411666 2216 64-65 -0.7003342013321046 2216 66-67 -0.6753161986547411 2216 68-69 -0.6703030815442048 2216 70-71 -0.5939339664027585 2216 72-73 -0.5942171592915457 2216 74-75 -0.7479695754396332 2216 76-77 -0.6200385158594699 2216 78-79 -0.7414779608050637 2216 80-81 -0.6999273228936218 2216 82-83 -0.6389930389724583 2216 84-85 -0.7493312346952692 2216 86-87 -0.7266685168513973 2216 88-89 -0.8613169293515099 2216 90-91 -0.7948085726132135 2216 92-93 -0.8730504376885335 2216 94-95 -0.7553749149344995 2216 96-97 -0.8707620125998368 2216 98-99 -0.9580953708448838 2216 100 -0.9765632392788177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18.0 3 7.0 4 13.0 5 41.0 6 112.0 7 265.0 8 555.0 9 1027.0 10 1839.0 11 2911.0 12 4316.0 13 6359.0 14 7473.0 15 9794.0 16 11820.0 17 16962.0 18 19274.0 19 10271.0 20 7909.0 21 7773.0 22 8336.0 23 9092.0 24 10983.0 25 13637.0 26 17248.0 27 22516.0 28 29101.0 29 37563.0 30 48307.0 31 61907.0 32 79424.0 33 103263.0 34 143773.0 35 221520.0 36 416214.0 37 914712.0 38 1483044.0 39 578587.0 40 1035.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27201100672225 19.231187137031 13.283405917459797 24.213395938786945 2 16.120344619421655 20.166028676411578 39.14738447258503 24.56624223158174 3 18.61944267226564 24.862197465097104 29.85715787595068 26.66120198668657 4 12.800611759900486 16.5357355043538 37.831478249568335 32.83217448617739 5 13.17200720168095 37.576311315355746 35.330288229688875 13.921393253274431 6 31.734856162582613 37.359645411681655 17.249141737624786 13.656356688110947 7 27.93048783233051 32.24371495852519 21.956342084859113 17.86945512428519 8 25.968840501175332 35.24110400787562 20.040167176027985 18.749888314921055 9 25.87198285635116 15.823482055353432 20.551816998882106 37.7527180894133 10-11 24.66465922870288 25.93358049188032 28.456744353355496 20.945015926061302 12-13 25.51548491169995 23.368432729535222 28.480893831308002 22.63518852745683 14-15 22.569179252571427 25.037160706217033 25.968597723780558 26.425062317430985 16-17 22.029742434140676 28.514143089049764 26.42931820528917 23.02679627152039 18-19 21.905385629008777 27.210520062028515 28.870887489539303 22.01320681942341 20-21 23.090560897990045 26.555551971326995 28.445050256428345 21.90883687425461 22-23 22.692681240057137 26.291838352098345 28.504187171254554 22.51129323658997 24-25 22.474940783048094 26.484392287807907 28.49102105887441 22.54964587026959 26-27 22.2729829746804 26.457764851842448 28.571773365198467 22.697478808278685 28-29 22.315494863358428 26.563343983541397 28.585278345228033 22.53588280787214 30-31 22.566309812505068 26.947782389704887 28.17931042645312 22.306597371336924 32-33 22.27941000709909 26.608046737515263 28.632970844054107 22.479572411331535 34-35 22.449356591005664 26.390362870651458 28.542891960340693 22.61738857800219 36-37 22.750447261441806 26.738343759957356 28.01490415063724 22.496304827963602 38-39 22.597650824402223 26.940687644305488 28.12926940606419 22.332392125228097 40-41 22.495517229815697 26.60048500829137 28.305034220828805 22.598963541064123 42-43 22.501503248664832 26.58221708465605 28.11845483442682 22.7978248322523 44-45 22.574025858893975 26.737090569252597 28.02318913362981 22.665694438223618 46-47 22.69203465025884 26.925301247319272 27.748589522258175 22.634074580163706 48-49 22.762062482696106 26.910135319068157 27.5175266842593 22.810275513976443 50-51 22.762317762284113 26.885106315825873 27.42732016075188 22.92525576113814 52-53 22.80798960130202 26.848763785387842 27.417770383436903 22.925476229873233 54-55 22.807687907243466 27.16109836131391 27.246686645001937 22.784527086440686 56-57 22.61991816664698 26.919081708266024 27.417004544672885 23.043995580414116 58-59 22.549368171416067 26.52636887297079 27.746988223024317 23.177274732588828 60-61 22.758929505934205 26.920810647293887 27.383423675232383 22.93683617153953 62-63 22.592313160289358 26.93399235692914 27.406468459858797 23.067226022922714 64-65 22.646351207623297 26.69336813799765 27.457408341283745 23.20287231309531 66-67 22.481881531241232 26.771251619574933 27.407779668651738 23.3390871805321 68-69 22.862701996197718 26.861015290815114 27.30594533477899 22.970337378208175 70-71 22.797313250710896 26.653591704245 27.39232476034457 23.156770284699533 72-73 22.68818107639195 26.81749171731274 27.396479173300087 23.09784803299522 74-75 22.719837957461976 26.81813983409577 27.232006928766634 23.230015279675616 76-77 22.69187219961193 26.7630130510529 27.209392617917704 23.33572213141747 78-79 22.824629188992994 26.901687885428665 27.23812317518608 23.03555975039226 80-81 22.728644063902514 26.65485573106156 27.35540093863984 23.261099266396084 82-83 22.587246882595668 26.706816800721562 27.443995912270463 23.261940404412307 84-85 22.56243384487495 26.654948560002655 27.223502616963884 23.55911497815851 86-87 22.615319938282372 26.7700043595009 27.19292352168303 23.421752180533698 88-89 22.38544467668923 26.692054362221178 27.31613308836814 23.60636787272145 90-91 22.357878955607156 26.57665560819835 27.34454033091857 23.72092510527592 92-93 22.428128465878064 26.754460981769903 27.228738727302304 23.58867182504973 94-95 22.219595922745487 26.427020513913046 27.419854545555815 23.933529017785656 96-97 22.226273183939487 26.4733120205301 27.35908793486549 23.941326860664926 98-99 22.133994616840962 26.607068842518693 27.303043355363222 23.955893185277123 100 22.2959335586137 26.386904992595735 27.2465474015903 24.070614047200266 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 26.0 1 30.5 2 102.0 3 179.5 4 127.5 5 90.0 6 246.0 7 586.0 8 987.0 9 1190.5 10 1000.5 11 767.0 12 787.5 13 951.0 14 1128.5 15 1286.5 16 1498.5 17 1792.5 18 2096.5 19 2403.0 20 2817.5 21 3453.0 22 4310.5 23 5668.0 24 7728.5 25 10348.5 26 14149.0 27 19375.5 28 26295.5 29 35070.5 30 44782.0 31 55782.0 32 67727.5 33 81083.0 34 93797.5 35 105194.0 36 116905.5 37 128449.5 38 139196.0 39 146007.0 40 151253.0 41 156506.0 42 159138.5 43 160857.0 44 160409.0 45 159406.0 46 159341.5 47 156264.5 48 152771.0 49 149776.5 50 146139.5 51 142269.0 52 134864.0 53 128529.0 54 124502.5 55 119090.5 56 111641.5 57 103002.0 58 94166.0 59 85549.0 60 76694.5 61 66174.5 62 56617.5 63 48033.0 64 39611.0 65 32373.0 66 26036.0 67 20104.5 68 16065.0 69 12964.5 70 9507.0 71 6779.5 72 5096.5 73 3760.0 74 2689.5 75 1898.0 76 1318.5 77 874.0 78 594.5 79 413.5 80 279.0 81 139.0 82 54.0 83 20.5 84 8.0 85 4.5 86 3.5 87 2.0 88 0.5 89 0.5 90 1.0 91 1.5 92 0.5 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04212113202108795 2 0.24010205613783797 3 0.0027384537622525498 4 0.00280807546807253 5 1.6245064691328687E-4 6 9.282894109330677E-5 7 0.0 8 2.3207235273326696E-4 9 0.0 10-11 6.03388117106494E-4 12-13 0.0 14-15 3.2490129382657375E-4 16-17 1.3924341163996018E-4 18-19 3.481085290999004E-4 20-21 0.0 22-23 1.3924341163996018E-4 24-25 0.001334416028216285 26-27 1.6245064691328687E-4 28-29 1.9726149982327688E-4 30-31 1.0443255872997013E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.801808818331674E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.0886511745994027E-4 70-71 4.6414470546653386E-5 72-73 3.01694058553247E-4 74-75 2.0886511745994027E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.740542645499502E-4 84-85 0.0 86-87 4.989555583765239E-4 88-89 0.0020770475569627392 90-91 0.0019958222335060955 92-93 6.498025876531475E-4 94-95 5.33766411286514E-4 96-97 2.436759703699303E-4 98-99 1.6245064691328687E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4309001.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.844287367669324 #Duplication Level Percentage of deduplicated Percentage of total 1 68.4036345597615 34.7793405254955 2 16.22131508963232 16.495224117975525 3 6.2737303841899115 9.56950051563091 4 3.074823093725231 6.2534875592844665 5 1.696889756438386 4.3138575203803855 6 1.0794712227061851 3.293096703144159 7 0.6681856390953211 2.378139585437861 8 0.48432220751207555 1.9700013997830355 9 0.3554756235090849 1.6266514278507611 >10 1.6406647858426116 14.389664692466079 >50 0.07740812524292057 2.672904935339336 >100 0.023440641342916634 1.81330051142287 >500 4.563365406768024E-4 0.13812212486701322 >1k 1.3690084567085837E-4 0.1851062457822962 >5k 4.563361522361935E-5 0.12160213513976101 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5241 0.1216291200675052 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4438 0.10299371014302386 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 2.3207235273326693E-5 0.0 1.1603617636663346E-5 0.0 12-13 0.0 2.3207235273326693E-5 0.0 2.3207235273326693E-5 0.0 14-15 0.0 4.641447054665339E-5 0.0 4.6414470546653386E-5 0.0 16-17 0.0 6.962170581998009E-5 0.0 4.6414470546653386E-5 0.0 18-19 0.0 6.962170581998009E-5 0.0 4.6414470546653386E-5 0.0 20-21 0.0 6.962170581998009E-5 0.0 5.801808818331674E-5 0.0 22-23 2.3207235273326693E-5 6.962170581998009E-5 0.0 8.122532345664342E-5 0.0 24-25 2.3207235273326693E-5 6.962170581998009E-5 0.0 1.740542645499502E-4 0.0 26-27 2.3207235273326693E-5 6.962170581998009E-5 0.0 2.6688320564325697E-4 0.0 28-29 2.3207235273326693E-5 8.122532345664342E-5 0.0 9.631002638430578E-4 0.0 30-31 2.3207235273326693E-5 9.282894109330677E-5 0.0 0.003747968496642261 0.0 32-33 2.3207235273326693E-5 9.282894109330677E-5 0.0 0.011267112725200111 0.0 34-35 2.3207235273326693E-5 1.1603617636663347E-4 0.0 0.022696676097313507 0.0 36-37 2.3207235273326693E-5 1.6245064691328685E-4 0.0 0.04374563849022082 0.0 38-39 2.3207235273326693E-5 2.0886511745994024E-4 0.0 0.07812715754765431 0.0 40-41 2.3207235273326693E-5 2.2046873509660357E-4 0.0 0.11761426836521968 0.0 42-43 2.3207235273326693E-5 2.7848682327992035E-4 0.0 0.159723796768671 0.0 44-45 2.3207235273326693E-5 2.7848682327992035E-4 0.0 0.2123345991333026 0.0 46-47 2.3207235273326693E-5 3.01694058553247E-4 0.0 0.27653741551696087 0.0 48-49 2.3207235273326693E-5 3.2490129382657375E-4 0.0 0.3454513006611045 0.0 50-51 2.3207235273326693E-5 3.481085290999004E-4 0.0 0.4224180964450925 0.0 52-53 2.3207235273326693E-5 3.713157643732271E-4 0.0 0.49943130669962715 0.0 54-55 2.3207235273326693E-5 3.713157643732271E-4 0.0 0.5874215392384452 0.0 56-57 2.3207235273326693E-5 3.713157643732271E-4 0.0 0.6942096323486581 0.0 58-59 2.3207235273326693E-5 3.713157643732271E-4 0.0 0.8151773462108735 0.0 60-61 2.3207235273326693E-5 4.0612661728321714E-4 0.0 0.9433276993901835 0.0 62-63 2.3207235273326693E-5 4.177302349198805E-4 0.0 1.0720814406866 0.0 64-65 2.3207235273326693E-5 4.5254108782987054E-4 0.0 1.2151772533819325 0.0 66-67 2.3207235273326693E-5 4.6414470546653387E-4 0.0 1.3765835747079196 0.0 68-69 2.3207235273326693E-5 4.6414470546653387E-4 0.0 1.5648058563922358 0.0 70-71 2.3207235273326693E-5 5.105591760131873E-4 0.0 1.7682520844158542 0.0 72-73 2.3207235273326693E-5 5.569736465598407E-4 0.0 1.9792058530503938 0.0 74-75 2.3207235273326693E-5 5.569736465598407E-4 0.0 2.193582688887749 0.0 76-77 2.3207235273326693E-5 5.569736465598407E-4 0.0 2.432350328997371 0.0 78-79 2.3207235273326693E-5 5.685772641965041E-4 0.0 2.705986840105166 0.0 80-81 2.3207235273326693E-5 5.917844994698307E-4 0.0 3.007495240776226 0.0 82-83 2.3207235273326693E-5 6.03388117106494E-4 0.0 3.313679899354862 0.0 84-85 2.3207235273326693E-5 6.03388117106494E-4 0.0 3.6266642778685823 0.0 86-87 2.3207235273326693E-5 6.03388117106494E-4 0.0 3.9711872891187543 0.0 88 2.3207235273326693E-5 6.03388117106494E-4 0.0 4.2496393015457645 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5205 0.0 49.43322 1 GTATCAA 9495 0.0 44.121857 1 TCAACGC 11295 0.0 36.574966 4 ATCAACG 11370 0.0 36.251034 3 CAACGCA 11465 0.0 36.032642 5 AACGCAG 12060 0.0 34.33285 6 TATCAAC 12420 0.0 33.526306 2 ACGCAGA 14315 0.0 28.858837 7 GTACATG 12875 0.0 27.817194 1 CGCAGAG 14795 0.0 27.763725 8 TACATGG 13105 0.0 26.166725 2 GCAGAGT 16555 0.0 24.613379 9 TACACCG 580 0.0 24.309418 5 ACATGGG 13610 0.0 24.207012 3 ACACCGT 830 0.0 20.95101 6 CATGGGG 9925 0.0 20.598711 4 GAGTACT 9880 0.0 20.57325 12-13 AGAGTAC 14630 0.0 20.55959 10-11 GAGTACA 9395 0.0 20.314457 1 CAGAGTA 15610 0.0 20.096811 10-11 >>END_MODULE