FastQCFastQC Report
Wed 25 May 2016
SRR1033009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262223
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29000.035.991551
TCAACGC27000.035.518484
ATCAACG27150.035.495393
GGTATCA17600.035.2619931
CAACGCA27750.034.5600475
AACGCAG28500.033.4774676
TATCAAC32300.030.5636162
GTACATG62150.027.1580721
ACGCAGA35350.026.7249977
CGCAGAG36350.025.6228548
TACATGG62850.024.8325162
ACATGGG62350.024.277693
ATATGCG1252.4210381E-422.564684
CATGGGG46050.021.5397554
TATGCGA1354.07181E-420.8941465
GCAGAGT46500.018.9200699
TATAGCG1550.001032306818.1973232
GTATAGA10600.018.185431
GAGTACA45350.017.8318631
GTCGTAT1350.00997417917.413331
Produced by FastQC (version 0.11.5)