##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033009_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2262223
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.623386819071328	33.0	31.0	34.0	30.0	34.0
2	31.630734016938206	33.0	31.0	34.0	30.0	34.0
3	31.804926393198194	34.0	31.0	34.0	30.0	34.0
4	35.20838838611401	37.0	35.0	37.0	33.0	37.0
5	35.23075399728497	37.0	35.0	37.0	33.0	37.0
6	35.30828083703508	37.0	35.0	37.0	33.0	37.0
7	35.183380241470445	37.0	35.0	37.0	33.0	37.0
8	35.15489321786579	37.0	35.0	37.0	33.0	37.0
9	36.79589722144988	39.0	38.0	39.0	33.0	39.0
10-11	36.82650538872605	39.0	38.0	39.0	34.0	39.0
12-13	36.783415251281596	39.0	38.0	39.0	33.0	39.0
14-15	38.16880409225793	41.0	39.0	41.0	34.0	41.0
16-17	38.157070943050265	40.5	38.5	41.0	33.5	41.0
18-19	38.14332053029255	40.0	38.5	41.0	33.5	41.0
20-21	38.179544633751846	40.0	39.0	41.0	34.0	41.0
22-23	38.12412503099827	40.0	38.5	41.0	33.5	41.0
24-25	38.13026257800402	40.0	38.0	41.0	33.5	41.0
26-27	38.05149492335636	40.0	38.0	41.0	33.0	41.0
28-29	38.07084602181129	40.0	38.0	41.0	33.0	41.0
30-31	38.2248082969716	40.0	39.0	41.0	33.5	41.0
32-33	38.218894865802355	40.0	39.0	41.0	33.0	41.0
34-35	38.172835967099616	40.0	38.0	41.0	33.0	41.0
36-37	38.09317892179507	40.0	38.0	41.0	33.0	41.0
38-39	38.00054526012688	40.0	38.0	41.0	33.0	41.0
40-41	37.92485886669881	40.0	38.0	41.0	33.0	41.0
42-43	37.78805029389234	40.0	38.0	41.0	32.5	41.0
44-45	37.628887382013176	40.0	38.0	41.0	32.0	41.0
46-47	37.48825579971559	40.0	38.0	41.0	32.0	41.0
48-49	37.36382664308515	40.0	37.0	41.0	31.5	41.0
50-51	37.229124847550395	40.0	37.0	41.0	31.0	41.0
52-53	37.071408963660964	40.0	37.0	41.0	31.0	41.0
54-55	36.90558247352273	40.0	36.0	41.0	31.0	41.0
56-57	36.72882779460734	40.0	36.0	41.0	30.0	41.0
58-59	36.529701757961085	39.0	35.5	41.0	30.0	41.0
60-61	36.29987958746773	39.0	35.0	41.0	30.0	41.0
62-63	36.04090357139857	39.0	35.0	41.0	30.0	41.0
64-65	35.76449182949692	38.5	35.0	40.0	29.5	41.0
66-67	35.46634814516517	38.0	35.0	40.0	29.0	41.0
68-69	35.14649086319076	37.0	34.5	40.0	29.0	41.0
70-71	34.81378537836456	37.0	34.0	39.5	29.0	41.0
72-73	34.4677693136353	36.0	34.0	39.0	28.5	41.0
74-75	34.118870465024884	36.0	34.0	39.0	28.0	40.5
76-77	33.75883434126521	35.0	34.0	38.0	28.0	40.0
78-79	33.38269657765835	35.0	34.0	37.0	27.5	39.0
80-81	33.03650413774416	35.0	33.0	37.0	27.0	39.0
82-83	32.82690499566135	35.0	33.0	36.0	27.0	39.0
84-85	32.60742464381275	35.0	33.0	36.0	27.0	37.0
86-87	32.43749754113542	35.0	33.0	36.0	27.0	37.0
88-89	32.220260115824125	35.0	33.0	35.0	27.0	37.0
90-91	32.01817791614708	35.0	33.0	35.0	26.5	36.0
92-93	31.80557487038192	35.0	33.0	35.0	26.0	36.0
94-95	31.607563445336734	35.0	33.0	35.0	25.5	36.0
96-97	31.434355720015226	35.0	33.0	35.0	25.0	35.5
98-99	31.256113787190742	35.0	33.0	35.0	25.0	35.0
100	31.113055167417183	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.03544691876156847
1101	2	0.03160779545574499
1101	3	0.012123754663321051
1101	4	-0.0551553139509835
1101	5	-0.033014414929674274
1101	6	0.0793219121133859
1101	7	0.10195357402891148
1101	8	0.11673349105704744
1101	9	0.152527101026962
1101	10-11	0.11677461532772071
1101	12-13	0.13091372618431762
1101	14-15	0.26379529089965104
1101	16-17	0.28184488424930976
1101	18-19	0.2097083354063045
1101	20-21	0.15205172895008445
1101	22-23	0.149298120559763
1101	24-25	0.1526960872153964
1101	26-27	0.13618777646215108
1101	28-29	0.14797632908658898
1101	30-31	0.14605991348379632
1101	32-33	0.10568910834280132
1101	34-35	0.0720005764824947
1101	36-37	0.13152759532206915
1101	38-39	0.12420586366072683
1101	40-41	0.168693151158827
1101	42-43	0.15341704378949572
1101	44-45	0.20259095747548628
1101	46-47	0.12683276203712524
1101	48-49	0.1357535704090651
1101	50-51	0.07479445014674724
1101	52-53	0.032595985234074476
1101	54-55	0.0036745344908695188
1101	56-57	0.11837116708892381
1101	58-59	0.061999497915891766
1101	60-61	0.055155379741620436
1101	62-63	0.07202355611222089
1101	64-65	0.07412372857611871
1101	66-67	0.03278992037240869
1101	68-69	-0.009877092927219167
1101	70-71	-0.07274401996784263
1101	72-73	-0.09003581228072477
1101	74-75	-0.0559980828505644
1101	76-77	-0.04301347129062805
1101	78-79	0.00641220299496581
1101	80-81	-0.06843918474826438
1101	82-83	-0.06974653654845753
1101	84-85	-0.07516313238217265
1101	86-87	0.007949249552808624
1101	88-89	0.03722725752692213
1101	90-91	0.07130972127303892
1101	92-93	0.17939534544138525
1101	94-95	0.16329212147743277
1101	96-97	0.1656475797297574
1101	98-99	0.1574892960689276
1101	100	0.17934400004540052
1102	1	-0.06845630732635755
1102	2	-0.08275313775606108
1102	3	0.00920117061791359
1102	4	-0.12529480206632115
1102	5	-0.03329492877681872
1102	6	-0.05898739764855776
1102	7	-0.060482354403134764
1102	8	0.006174618048994773
1102	9	-0.03343709071786094
1102	10-11	-0.003924888892129275
1102	12-13	-0.051920100973433136
1102	14-15	-0.08617906529931929
1102	16-17	-0.021812619774280506
1102	18-19	-0.13192778967879804
1102	20-21	0.007236935865719829
1102	22-23	-0.09975275532524108
1102	24-25	-0.0031049249813506208
1102	26-27	-0.15679538157953488
1102	28-29	-0.04383545856452997
1102	30-31	0.06378291592273655
1102	32-33	0.015153542363371741
1102	34-35	0.04198493730443431
1102	36-37	0.06743159747473015
1102	38-39	0.13297644824834975
1102	40-41	-0.010912389520740362
1102	42-43	0.03707071388781458
1102	44-45	0.032366040602873625
1102	46-47	-0.03966292909618829
1102	48-49	-0.07505177644989658
1102	50-51	-0.05243289352976177
1102	52-53	-0.06071401746687144
1102	54-55	-0.029343452587077934
1102	56-57	-0.09100702890254553
1102	58-59	-0.06915898482081673
1102	60-61	-0.05644821261602573
1102	62-63	-0.08294431073829145
1102	64-65	-0.0018619897993161771
1102	66-67	-0.0025738928355139024
1102	68-69	-0.05833871776296462
1102	70-71	-0.05782929192869801
1102	72-73	-0.010303974616604705
1102	74-75	-0.07794583828457036
1102	76-77	-0.04304073363530847
1102	78-79	-0.11571935830281888
1102	80-81	-0.05111950315626501
1102	82-83	-0.1573912824016972
1102	84-85	-0.15809063275393243
1102	86-87	-0.23359552897478864
1102	88-89	-0.18903123603379868
1102	90-91	-0.10927276224057536
1102	92-93	-0.19737749094509738
1102	94-95	-0.12450906673592499
1102	96-97	-0.10693479655533977
1102	98-99	-0.051653857036196626
1102	100	-0.011209673391164188
1103	1	0.059748702604402126
1103	2	0.09859498543225342
1103	3	0.13165683391845917
1103	4	0.11354159813618026
1103	5	-0.013603118664782698
1103	6	0.1261410256958584
1103	7	0.17701444073107808
1103	8	0.11223366907046284
1103	9	0.19385179847854062
1103	10-11	0.23771757070734623
1103	12-13	0.25375567308574176
1103	14-15	0.27531518823698775
1103	16-17	0.2960948825138061
1103	18-19	0.20451123638026303
1103	20-21	0.182984856402733
1103	22-23	0.21993275076293628
1103	24-25	0.17199145162718565
1103	26-27	0.13948509059185454
1103	28-29	0.16277459873673195
1103	30-31	0.16471418169000174
1103	32-33	0.11747111679025579
1103	34-35	0.17177114248579528
1103	36-37	0.13038111954131182
1103	38-39	0.1604605507686827
1103	40-41	0.18944053971697628
1103	42-43	0.09337227763167988
1103	44-45	0.1881700861717377
1103	46-47	0.2711215067301964
1103	48-49	0.16004218617877086
1103	50-51	0.15479843685375982
1103	52-53	0.16543995524055077
1103	54-55	0.18248414542991753
1103	56-57	0.23171642087455524
1103	58-59	0.1278343609744894
1103	60-61	0.11161225834837296
1103	62-63	0.05947579248626056
1103	64-65	0.04604417096174984
1103	66-67	0.18305971729827775
1103	68-69	0.11283657522999846
1103	70-71	0.10089363200056312
1103	72-73	0.238963663100364
1103	74-75	0.12718431097857064
1103	76-77	0.184467931514547
1103	78-79	0.10559656439293974
1103	80-81	0.11858299304498843
1103	82-83	0.10995631493877056
1103	84-85	0.12545917076425184
1103	86-87	0.0387172559315303
1103	88-89	-0.009354736243096795
1103	90-91	0.05275333564938478
1103	92-93	0.1418847960394416
1103	94-95	0.059441676543080746
1103	96-97	0.01099676865306165
1103	98-99	0.004071984041669197
1103	100	0.00864550319261781
1104	1	-0.046543418070978504
1104	2	0.01778831532582359
1104	3	0.0492530396146833
1104	4	0.012883425203405352
1104	5	-0.06756783100415475
1104	6	0.004519523902175138
1104	7	0.12591561078550484
1104	8	0.05008594667430799
1104	9	0.12522709135237875
1104	10-11	0.08927955835709156
1104	12-13	0.12489220000242085
1104	14-15	0.11105201290912703
1104	16-17	0.13116379873234507
1104	18-19	0.16139839689092383
1104	20-21	0.07629486328040969
1104	22-23	0.11030810773974054
1104	24-25	0.05100118844114121
1104	26-27	-0.11792637482201229
1104	28-29	-0.10139363217134445
1104	30-31	-0.08100532964226659
1104	32-33	-0.055879622250316174
1104	34-35	0.03746062703338282
1104	36-37	0.06592952401506835
1104	38-39	-0.03360601876445202
1104	40-41	0.048847380481355174
1104	42-43	0.11577422828274564
1104	44-45	0.11257940145797107
1104	46-47	0.10060755436182944
1104	48-49	0.11245650297335885
1104	50-51	0.06587886352449601
1104	52-53	0.10077148112917911
1104	54-55	0.07609979593543414
1104	56-57	0.09067347872997544
1104	58-59	0.025227005613018605
1104	60-61	0.08388897731898481
1104	62-63	0.05414522537092381
1104	64-65	0.03451843196275206
1104	66-67	0.014165334398214213
1104	68-69	0.031124843447102535
1104	70-71	0.04794339048760321
1104	72-73	0.10127019271602933
1104	74-75	0.17456041262109778
1104	76-77	0.0591932078638564
1104	78-79	0.14664051334186468
1104	80-81	0.017278802725343212
1104	82-83	0.052767473360454176
1104	84-85	0.07984326791108032
1104	86-87	0.07115385912518946
1104	88-89	0.12325857496639969
1104	90-91	0.06567576165249989
1104	92-93	0.018698590995331443
1104	94-95	0.03653087404982713
1104	96-97	0.07952846183747653
1104	98-99	0.12180371114338229
1104	100	0.11947877325430412
1105	1	-0.16681864385438772
1105	2	-0.1036455990530385
1105	3	-0.08023149072462488
1105	4	-0.08346164169202552
1105	5	-0.108324669294241
1105	6	-0.02616002910934867
1105	7	-0.06467372229819546
1105	8	0.017318462376323396
1105	9	0.03506569295054618
1105	10-11	0.06805651736939922
1105	12-13	0.09982715213259041
1105	14-15	0.1316035081330753
1105	16-17	0.12645270929357366
1105	18-19	0.1274363923570121
1105	20-21	0.08993446590795884
1105	22-23	0.0761487867797328
1105	24-25	0.04673616546394044
1105	26-27	0.028937955568800078
1105	28-29	0.004457529276244543
1105	30-31	0.015779721591243856
1105	32-33	0.010380661606795627
1105	34-35	-0.09983202764779975
1105	36-37	0.03550286393473101
1105	38-39	-0.011806498247331376
1105	40-41	-0.004015395706552738
1105	42-43	-0.006112187268030311
1105	44-45	0.011295209566796416
1105	46-47	0.02285470153741187
1105	48-49	0.0072286710352926775
1105	50-51	-0.04606605676530506
1105	52-53	-0.044356182642566466
1105	54-55	-0.04743280362936986
1105	56-57	-0.04331160261446598
1105	58-59	-0.033153975428206195
1105	60-61	-0.05209562415472391
1105	62-63	-0.05698945029588032
1105	64-65	-0.01992538781529163
1105	66-67	-0.07319877201179281
1105	68-69	-0.025525288658371892
1105	70-71	-0.0842817535044631
1105	72-73	-0.04625395992518122
1105	74-75	-0.0965315982717172
1105	76-77	-0.18497875157243016
1105	78-79	-0.167709322643546
1105	80-81	-0.061563807961810824
1105	82-83	-0.07258394556370007
1105	84-85	-0.07032171629919759
1105	86-87	-0.12177237564723953
1105	88-89	-0.2201579935488951
1105	90-91	-0.1856593966704665
1105	92-93	-0.16931456613624363
1105	94-95	-0.22284251561983837
1105	96-97	-0.1536330030269788
1105	98-99	-0.22687878632107683
1105	100	-0.23431949522684548
1106	1	-0.10847075046563504
1106	2	-0.05488254097103251
1106	3	-0.04640153335715169
1106	4	-0.0684161655717972
1106	5	-0.10464065456741878
1106	6	-0.09806002012958004
1106	7	-0.0069713460800500116
1106	8	-0.030541487422482305
1106	9	-0.09610514075693999
1106	10-11	-0.05555065618389676
1106	12-13	-0.018498676678788684
1106	14-15	-0.0432782796108242
1106	16-17	-0.012006276676601146
1106	18-19	0.03619419085952558
1106	20-21	0.03926491716276814
1106	22-23	0.025971115618901308
1106	24-25	0.013301018644703788
1106	26-27	0.03026251438415528
1106	28-29	0.0031618767676775406
1106	30-31	0.008110784325623399
1106	32-33	0.013698258962463683
1106	34-35	-0.010305945692437035
1106	36-37	0.017685632183969346
1106	38-39	0.06777106772189967
1106	40-41	0.11243028309991132
1106	42-43	0.038808598333559985
1106	44-45	-0.01633691064238718
1106	46-47	-0.05967549828385188
1106	48-49	-0.04737273408488818
1106	50-51	-0.11597356513573231
1106	52-53	-0.12310440272390366
1106	54-55	-0.13706459030424156
1106	56-57	-0.13136504507338742
1106	58-59	-0.0915481360121504
1106	60-61	-0.1196007020007741
1106	62-63	-0.1239544163651729
1106	64-65	-0.07429319063665218
1106	66-67	-0.11344075542915277
1106	68-69	-0.1580390683290318
1106	70-71	-0.161223201128891
1106	72-73	-0.14048497091898327
1106	74-75	-0.20553689633593564
1106	76-77	-0.13735612426280852
1106	78-79	-0.15031597879104908
1106	80-81	-0.16910189715230928
1106	82-83	-0.12277555674821627
1106	84-85	-0.13163071018012573
1106	86-87	-0.15725174569500666
1106	88-89	-0.15958016673872066
1106	90-91	-0.19110434426709588
1106	92-93	-0.11604255180282053
1106	94-95	-0.17358086650648374
1106	96-97	-0.17934328144260547
1106	98-99	-0.1719542377683183
1106	100	-0.22370070356178218
1107	1	0.06860746667001294
1107	2	0.16650793055600488
1107	3	0.13441088909588572
1107	4	0.12350509028442502
1107	5	0.10058137354244678
1107	6	0.1373425601354228
1107	7	0.15165751985654907
1107	8	0.1704862199261683
1107	9	0.1923851676880517
1107	10-11	0.17815587083252638
1107	12-13	0.19652051554638206
1107	14-15	0.2312884325638933
1107	16-17	0.20548545510531113
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2216	2	0.0632979426565825
2216	3	0.07235922185224553
2216	4	0.005317064457159404
2216	5	0.13668667080060004
2216	6	-0.06614834537526804
2216	7	0.05776624719973
2216	8	0.1759834075740514
2216	9	0.12899211488062434
2216	10-11	0.050398860596267525
2216	12-13	-0.027652532242953498
2216	14-15	-0.06472814300437335
2216	16-17	-0.06282754650491995
2216	18-19	-0.006038814890175104
2216	20-21	0.03283258832861691
2216	22-23	0.04078781830617828
2216	24-25	0.1346093822217611
2216	26-27	0.10211159483923637
2216	28-29	0.11070071785563584
2216	30-31	-0.025209975777130467
2216	32-33	-0.03230039536204288
2216	34-35	-0.17879206076807463
2216	36-37	-0.14661971381773498
2216	38-39	-0.06661032933392619
2216	40-41	0.032568928453251544
2216	42-43	-0.0028944967200672522
2216	44-45	0.05899094484021106
2216	46-47	0.16461333260959066
2216	48-49	0.0668622697890271
2216	50-51	0.19746076182263295
2216	52-53	0.1057250634914837
2216	54-55	0.07108796036247611
2216	56-57	0.06243281815705615
2216	58-59	0.056929823454474615
2216	60-61	0.13845765138176347
2216	62-63	0.026023971270923596
2216	64-65	-0.035398071768646844
2216	66-67	-0.010859505174288131
2216	68-69	-0.029441221409811646
2216	70-71	-0.045062861855555525
2216	72-73	-0.05422859934942892
2216	74-75	-0.058732263950012964
2216	76-77	0.09268363433115923
2216	78-79	-0.0022449842145348953
2216	80-81	0.07006949297124976
2216	82-83	-0.018633730707357188
2216	84-85	0.10445663639247726
2216	86-87	0.20851467766265586
2216	88-89	0.14958612612920064
2216	90-91	0.1605512443639867
2216	92-93	0.06649812429716917
2216	94-95	0.1081430253501452
2216	96-97	0.13878500628727863
2216	98-99	0.16061110904895415
2216	100	0.17620142912997494
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24515.0
3	5203.0
4	2922.0
5	2494.0
6	2042.0
7	2144.0
8	2482.0
9	2868.0
10	3216.0
11	3422.0
12	3969.0
13	4438.0
14	4822.0
15	5062.0
16	4782.0
17	5042.0
18	4843.0
19	4914.0
20	4769.0
21	4946.0
22	5762.0
23	8250.0
24	12932.0
25	17224.0
26	17554.0
27	15334.0
28	16722.0
29	20848.0
30	25659.0
31	31452.0
32	41678.0
33	56799.0
34	81944.0
35	129008.0
36	237098.0
37	473153.0
38	700973.0
39	270482.0
40	456.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.03133322452359	19.73706966521543	13.33401450000376	23.89758261025722
2	15.500760426511045	20.118510669630833	40.32073680221083	24.05999210164729
3	18.240113937683695	25.16089938232152	29.775110189151594	26.823876490843183
4	12.182701818896064	16.656683589236327	39.0334935934301	32.127120998437505
5	12.888755173779254	38.101225779191616	35.542862452133555	13.467156594895574
6	31.05765172169379	38.08056781871002	17.826054638658007	13.035725820938183
7	27.596421575849927	32.30930830082372	22.457580435564605	17.63668968776174
8	24.943678818589373	36.876423517583575	20.261686064340893	17.91821159948615
9	24.83287166915936	17.398515004823135	21.028832623874973	36.73978070214253
10-11	23.784398402164435	27.190751245570972	28.77110744413129	20.253742908133304
12-13	24.41567723291927	24.505091361280872	28.738953228511622	22.340278177288237
14-15	21.861306730130128	26.46100634664113	25.732085187129	25.94560173609974
16-17	21.66246149374732	29.76278135526526	26.246582905314046	22.328174245673377
18-19	21.549234323511204	28.76031518250611	28.065416255696608	21.62503423828608
20-21	22.704327867039076	28.090174497386688	27.60107297917646	21.60442465639778
22-23	22.398357612859378	27.836940485581675	27.61372852002262	22.150973381536325
24-25	22.203608387013908	27.88602319832113	27.63082353841049	22.279544876254473
26-27	22.002825590639237	27.82577886431629	27.72787967767008	22.44351586737439
28-29	22.015096614751457	27.915533447412766	27.79147925282616	22.277890685009613
30-31	22.252036875892244	28.336828954627112	27.347157442753577	22.06397672672706
32-33	21.8962650297022	28.0383321824625	27.886116428021325	22.17928635981398
34-35	22.046740860051987	27.67429336703	27.99561577842261	22.283349994495406
36-37	22.369035008768225	28.072980820475852	27.390806300737946	22.16717787001798
38-39	22.12947151414339	28.247029313136494	27.566602666761042	22.05689650595907
40-41	21.972136816684728	27.926560012423156	27.855352477298865	22.245950693593247
42-43	21.989490578858703	27.829439120032845	27.78593004572545	22.395140255383
44-45	22.061834645618784	28.049833299896726	27.768368586281817	22.119963468202673
46-47	22.07002501841541	28.170315462088585	27.625020185061068	22.13463933443494
48-49	21.95282023059452	27.9750692128624	27.718059226213914	22.35405133032917
50-51	22.00468030798019	27.91870066156905	27.70645002178688	22.37016900866388
52-53	22.100332791031786	27.820909982735564	27.722654232440547	22.356102993792103
54-55	22.177264531699816	28.26431317801476	27.516318358839342	22.042103931446082
56-57	21.93500754887749	27.998837477290333	27.61522267750921	22.45093229632297
58-59	21.965283528320985	27.58716938800065	27.967899583129853	22.479647500548513
60-61	22.157073894216424	28.0538195815217	27.547016163081224	22.24209036118065
62-63	21.981052178504022	28.005157091564918	27.603334813044373	22.410455916886686
64-65	22.036940376083198	27.76791836048605	27.748565690702314	22.446575572728438
66-67	21.88636712493105	27.872881509631604	27.56809934925249	22.67265201618486
68-69	22.339232584248787	27.95763208951857	27.49935194941578	22.20378337681686
70-71	22.205510059697144	27.76636971800382	27.605278330488137	22.4228418918109
72-73	21.94425799888317	27.88433617701209	27.782472397149316	22.388933426955422
74-75	22.091427552823774	27.963232410892537	27.634056242592486	22.311283793691203
76-77	22.0081488482852	27.943375588750307	27.529851614355668	22.518623948608827
78-79	22.150229133362185	28.009311728625075	27.582446767929248	22.258012370083495
80-81	22.06267862967201	27.810272484534888	27.67969661287737	22.447352272915733
82-83	21.94839698680933	27.871925243432973	27.827630526777625	22.35204724298007
84-85	21.98151444460414	27.805149863458965	27.67172660836493	22.54160908357196
86-87	22.177606498604444	27.885090140099518	27.61064447633879	22.32665888495725
88-89	21.89883930632566	27.84612295764171	27.660560561180315	22.594477174852315
90-91	21.904214183245507	27.676605127279867	27.773141834223964	22.64603885525066
92-93	22.141059449476856	27.892661121309587	27.543979363452912	22.42230006576064
94-95	21.839291151264504	27.66171501753084	27.75601915800222	22.742974673202436
96-97	21.906504122567895	27.63608631123486	27.80232459406725	22.65508497212999
98-99	21.854982349032543	27.741660152816582	27.691841637709057	22.71151586044182
100	22.007908823411036	27.60892323328552	27.666905939313203	22.716262003990238
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	428.0
1	408.5
2	355.5
3	334.0
4	333.5
5	314.0
6	298.5
7	277.5
8	253.5
9	254.0
10	237.0
11	202.5
12	222.0
13	250.0
14	258.0
15	310.5
16	393.5
17	507.0
18	678.5
19	950.0
20	1378.0
21	1982.0
22	2956.5
23	4497.0
24	6573.0
25	9115.0
26	12449.0
27	16800.5
28	22216.0
29	28074.5
30	34320.0
31	41191.5
32	47666.0
33	54335.0
34	60597.0
35	65817.0
36	70330.0
37	74126.0
38	77550.5
39	79051.0
40	80177.0
41	81646.0
42	81436.0
43	80464.0
44	79254.0
45	78098.0
46	77154.5
47	75273.5
48	72702.5
49	70915.0
50	69862.5
51	68428.0
52	65264.0
53	62115.0
54	60005.0
55	57074.0
56	52709.5
57	47579.5
58	43119.0
59	39719.5
60	36112.5
61	31225.5
62	26731.0
63	22728.0
64	18573.5
65	15192.0
66	12231.0
67	9339.5
68	7160.5
69	5574.0
70	4083.5
71	3072.0
72	2500.0
73	1923.0
74	1402.0
75	1018.0
76	719.0
77	511.5
78	354.0
79	237.0
80	155.5
81	90.5
82	58.0
83	37.5
84	27.0
85	22.0
86	13.0
87	8.0
88	9.0
89	9.5
90	6.0
91	2.0
92	1.5
93	1.0
94	0.5
95	1.0
96	1.0
97	1.0
98	1.5
99	2.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06170921257541807
2	0.04385067254642889
3	0.059145362769276066
4	0.07651765542123831
5	0.08964633460096551
6	0.0912818939600561
7	0.09380153945919567
8	0.10679760571791552
9	0.10091843288658987
10-11	0.10679760571791552
12-13	0.11108542349715302
14-15	0.10807953062098652
16-17	0.07574408004869547
18-19	0.10281921808769515
20-21	0.09550340527878994
22-23	0.06044938982584829
24-25	0.020532900602637315
26-27	0.18954806842649907
28-29	0.06708003587621557
30-31	0.07850684923634849
32-33	0.07143416011595674
34-35	0.021041250133165476
36-37	0.053840845928982244
38-39	0.0797003655254146
40-41	0.08524800605422189
42-43	0.07846264492934604
44-45	0.07631873603972729
46-47	0.08345773162062271
48-49	0.0797887741394195
50-51	0.07461687022013302
52-53	0.07936883322289624
54-55	0.06979860075686614
56-57	0.07169938595797143
58-59	0.06926814907283677
60-61	0.06562129374513477
62-63	0.07278239147953142
64-65	0.06864928877480249
66-67	0.07037325674789797
68-69	0.07059427828291022
70-71	0.061642906114914395
72-73	0.060139959676831146
74-75	0.06310164824599519
76-77	0.057023556033158534
78-79	0.053199883477446745
80-81	0.04798377525115782
82-83	0.05441550192001408
84-85	0.04411589838844358
86-87	0.04144153781479545
88-89	0.043718059625421545
90-91	0.040093306451220764
92-93	0.04385067254642889
94-95	0.04077847320975872
96-97	0.04469055437947541
98-99	0.044889473760986426
100	0.05322198563094796
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2262223.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.91235057655946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.16386199407268	55.24280161306467
2	12.464089465860104	17.178594057779062
3	3.4953362560921892	7.226155123883513
4	1.4633108589570836	4.033607636597476
5	0.7517713837483374	2.5903166575145318
6	0.44125934516993875	1.8244931213720326
7	0.2664848515602103	1.2854868259841734
8	0.1877206382908717	1.034901634908485
9	0.1232093548915599	0.7641581632739016
>10	0.6132163320508435	7.184423499713681
>50	0.025245123121122984	1.158176965342652
>100	0.0044303527951251	0.4476239876218216
>500	6.404338986564129E-5	0.029260712943949455
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	4.420430700244848E-5	0.0
12-13	0.0	0.0	0.0	8.840861400489696E-5	0.0
14-15	0.0	0.0	0.0	8.840861400489696E-5	0.0
16-17	0.0	0.0	0.0	1.3261292100734542E-4	0.0
18-19	0.0	0.0	0.0	1.3261292100734542E-4	0.0
20-21	0.0	0.0	0.0	1.5471507450856965E-4	0.0
22-23	0.0	0.0	0.0	2.431236885134666E-4	0.0
24-25	0.0	0.0	0.0	2.6522584201469084E-4	0.0
26-27	0.0	0.0	0.0	3.536344560195878E-4	0.0
28-29	0.0	0.0	0.0	9.945969075550907E-4	0.0
30-31	0.0	0.0	0.0	0.0035363445601958783	0.0
32-33	0.0	0.0	0.0	0.008531431251472555	0.0
34-35	0.0	0.0	0.0	0.016355593590905937	0.0
36-37	0.0	0.0	0.0	0.03171659027425678	0.0
38-39	0.0	0.0	0.0	0.057045658186659756	0.0
40-41	0.0	0.0	0.0	0.08661833957129779	0.0
42-43	0.0	0.0	0.0	0.11829072553855212	4.420430700244848E-5
44-45	0.0	0.0	0.0	0.15551075203461373	4.420430700244848E-5
46-47	0.0	0.0	0.0	0.2012401076286467	4.420430700244848E-5
48-49	0.0	0.0	0.0	0.2526718188259955	4.420430700244848E-5
50-51	0.0	0.0	0.0	0.31190559020927644	4.420430700244848E-5
52-53	0.0	0.0	0.0	0.37107305513205374	4.420430700244848E-5
54-55	0.0	0.0	0.0	0.43172136433941305	4.420430700244848E-5
56-57	0.0	0.0	0.0	0.5046142665864506	4.420430700244848E-5
58-59	0.0	0.0	0.0	0.5891771058821346	4.420430700244848E-5
60-61	0.0	0.0	0.0	0.6829344410343278	4.420430700244848E-5
62-63	0.0	0.0	0.0	0.780537550895734	4.420430700244848E-5
64-65	0.0	0.0	0.0	0.88481551111451	4.420430700244848E-5
66-67	0.0	0.0	0.0	1.0055374735381968	4.420430700244848E-5
68-69	0.0	0.0	0.0	1.1484499980771126	6.630646050367272E-5
70-71	0.0	0.0	0.0	1.3018389433756088	8.840861400489696E-5
72-73	0.0	0.0	0.0	1.4703236595154412	8.840861400489696E-5
74-75	0.0	0.0	0.0	1.6310284176228427	8.840861400489696E-5
76-77	0.0	0.0	0.0	1.8060111668920351	8.840861400489696E-5
78-79	0.0	0.0	0.0	2.0059693496176108	8.840861400489696E-5
80-81	0.0	0.0	0.0	2.235875950337345	8.840861400489696E-5
82-83	0.0	0.0	0.0	2.4776956117942395	8.840861400489696E-5
84-85	0.0	0.0	0.0	2.7215486713732466	8.840861400489696E-5
86-87	0.0	0.0	0.0	2.9802764802585777	8.840861400489696E-5
88	0.0	0.0	0.0	3.1882356425515965	8.840861400489696E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2900	0.0	35.99155	1
TCAACGC	2700	0.0	35.51848	4
ATCAACG	2715	0.0	35.49539	3
GGTATCA	1760	0.0	35.261993	1
CAACGCA	2775	0.0	34.560047	5
AACGCAG	2850	0.0	33.477467	6
TATCAAC	3230	0.0	30.563616	2
GTACATG	6215	0.0	27.158072	1
ACGCAGA	3535	0.0	26.724997	7
CGCAGAG	3635	0.0	25.622854	8
TACATGG	6285	0.0	24.832516	2
ACATGGG	6235	0.0	24.27769	3
ATATGCG	125	2.4210381E-4	22.56468	4
CATGGGG	4605	0.0	21.539755	4
TATGCGA	135	4.07181E-4	20.894146	5
GCAGAGT	4650	0.0	18.920069	9
TATAGCG	155	0.0010323068	18.197323	2
GTATAGA	1060	0.0	18.18543	1
GAGTACA	4535	0.0	17.831863	1
GTCGTAT	135	0.009974179	17.41333	1
>>END_MODULE