FastQCFastQC Report
Wed 25 May 2016
SRR1033009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262223
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44020.1945873594247782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36340.16063845164689777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27750.053.345611
GTATCAA54800.044.477671
ATCAACG66650.035.894083
TCAACGC67950.035.343364
CAACGCA69600.034.4371955
TATCAAC72000.033.5057372
AACGCAG72000.033.3545576
ACGCAGA84750.028.1148057
CGCAGAG87000.027.4417178
GTACATG60650.024.952051
GCAGAGT97350.024.0414249
TACATGG60600.024.0252322
GTGGTAT15100.022.7917611
ACATGGG62000.022.439093
ATAACGC2154.950016E-821.8608343
AGAGTAC90150.021.3478910-11
TGGTATC15550.020.3014072
GAGTACT69050.020.14627812-13
TAACGCC1200.00506706619.5823674
AGTACTT73100.019.5444312-13
Produced by FastQC (version 0.11.5)