##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033009_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2262223 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.61924973797897 34.0 31.0 34.0 31.0 34.0 2 32.62779045213491 34.0 31.0 34.0 31.0 34.0 3 32.730440367726786 34.0 31.0 34.0 31.0 34.0 4 36.24791941378016 37.0 37.0 37.0 35.0 37.0 5 36.23279800444077 37.0 37.0 37.0 35.0 37.0 6 36.303360013579564 37.0 37.0 37.0 35.0 37.0 7 36.25307761436428 37.0 37.0 37.0 35.0 37.0 8 36.26202810244613 37.0 37.0 37.0 35.0 37.0 9 38.04624521985675 39.0 39.0 39.0 35.0 39.0 10-11 38.004820479678614 39.0 38.0 39.0 35.0 39.0 12-13 37.9806749820862 39.0 38.0 39.0 35.0 39.0 14-15 39.44618258235373 41.0 39.0 41.0 36.0 41.0 16-17 39.43738791445406 41.0 39.0 41.0 36.0 41.0 18-19 39.456641542412044 41.0 39.0 41.0 36.0 41.0 20-21 39.45121480066289 41.0 39.0 41.0 36.0 41.0 22-23 39.37886649547812 41.0 39.0 41.0 36.0 41.0 24-25 39.318398760864866 41.0 39.0 41.0 36.0 41.0 26-27 39.20068224927428 41.0 39.0 41.0 36.0 41.0 28-29 39.14565761200377 40.5 39.0 41.0 36.0 41.0 30-31 39.13960626339667 41.0 39.0 41.0 36.0 41.0 32-33 39.09331485003909 41.0 39.0 41.0 35.0 41.0 34-35 39.0086711168616 41.0 39.0 41.0 35.0 41.0 36-37 38.91580538258165 41.0 39.0 41.0 35.0 41.0 38-39 38.81752638886617 41.0 39.0 41.0 35.0 41.0 40-41 38.70856918172965 41.0 38.0 41.0 35.0 41.0 42-43 38.582025954116816 40.5 38.0 41.0 35.0 41.0 44-45 38.40565673675849 40.0 38.0 41.0 34.5 41.0 46-47 38.19460636727679 40.0 38.0 41.0 34.0 41.0 48-49 37.97513021483735 40.0 38.0 41.0 33.5 41.0 50-51 37.826304701172255 40.0 38.0 41.0 33.0 41.0 52-53 37.68272424955453 40.0 37.5 41.0 33.0 41.0 54-55 37.521019369001195 40.0 37.0 41.0 33.0 41.0 56-57 37.367563012134525 40.0 37.0 41.0 33.0 41.0 58-59 37.178460965165684 40.0 36.0 41.0 32.5 41.0 60-61 36.9745535696525 39.5 36.0 41.0 32.0 41.0 62-63 36.73784503119277 39.0 35.0 41.0 32.0 41.0 64-65 36.46483127437038 39.0 35.0 41.0 31.5 41.0 66-67 36.18457022141495 38.5 35.0 41.0 31.0 41.0 68-69 35.874980052806464 37.5 35.0 40.0 31.0 41.0 70-71 35.55086921139074 37.0 35.0 40.0 31.0 41.0 72-73 35.21020208883032 36.5 35.0 39.0 30.5 41.0 74-75 34.87188354110094 36.0 35.0 39.0 30.0 41.0 76-77 34.5216727086587 35.5 34.5 38.5 30.0 40.0 78-79 34.16745077739905 35.0 34.0 37.0 30.0 39.0 80-81 33.84526613866095 35.0 34.0 37.0 29.5 39.0 82-83 33.54998976670293 35.0 34.0 36.5 29.0 39.0 84-85 33.33303878530101 35.0 34.0 36.0 29.0 37.0 86-87 33.12230381354977 35.0 34.0 36.0 29.0 37.0 88-89 32.912816508363676 35.0 34.0 35.5 29.0 37.0 90-91 32.73151320625774 35.0 34.0 35.0 29.0 36.0 92-93 32.551392590385646 35.0 34.0 35.0 29.0 36.0 94-95 32.39128436940125 35.0 34.0 35.0 29.0 36.0 96-97 32.23783884258979 35.0 34.0 35.0 29.0 36.0 98-99 32.10440394249373 35.0 34.0 35.0 29.0 35.0 100 31.994326819239305 35.0 34.0 35.0 28.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1375048506282397 1101 2 0.08035953756012049 1101 3 0.1421850118467134 1101 4 0.15202920369778639 1101 5 0.12199137381232816 1101 6 0.11814217592959864 1101 7 0.18868493340468717 1101 8 0.2025046966249633 1101 9 0.2732761562387225 1101 10-11 0.2204708193512701 1101 12-13 0.21988469063169447 1101 14-15 0.32974041126401943 1101 16-17 0.35475107475836865 1101 18-19 0.2723368128575103 1101 20-21 0.28353686553447943 1101 22-23 0.3577224089804858 1101 24-25 0.4104029690295192 1101 26-27 0.397397102442838 1101 28-29 0.42314776042583446 1101 30-31 0.4171996196069543 1101 32-33 0.43280727138562014 1101 34-35 0.3576728186412197 1101 36-37 0.3999634179718754 1101 38-39 0.3807874102897273 1101 40-41 0.4285963975507343 1101 42-43 0.4012840996183442 1101 44-45 0.3872017218181867 1101 46-47 0.4144097633199948 1101 48-49 0.4590005463653313 1101 50-51 0.514109102990524 1101 52-53 0.44861856760632435 1101 54-55 0.4835043891953674 1101 56-57 0.5094310449181378 1101 58-59 0.43587717936049586 1101 60-61 0.3696254750439536 1101 62-63 0.4156723594542129 1101 64-65 0.44496052298975997 1101 66-67 0.4586618626970065 1101 68-69 0.39813225037421063 1101 70-71 0.391145142257578 1101 72-73 0.3929914384381519 1101 74-75 0.3617964707311998 1101 76-77 0.39961436899661607 1101 78-79 0.419681975372427 1101 80-81 0.36375768181213886 1101 82-83 0.42517821553656887 1101 84-85 0.42114983183552823 1101 86-87 0.37567274543393836 1101 88-89 0.43100492074411534 1101 90-91 0.4339524150362877 1101 92-93 0.41128734069855 1101 94-95 0.43517294712746946 1101 96-97 0.42545570682804623 1101 98-99 0.4913658969860748 1101 100 0.4182835256633908 1102 1 0.1443222576606047 1102 2 0.10540296005160599 1102 3 0.16923178674674233 1102 4 0.14032986436707517 1102 5 0.08878867043979 1102 6 0.13487892890847775 1102 7 0.1518389894245118 1102 8 0.14338475950538765 1102 9 0.18587884524669107 1102 10-11 0.20794905752604365 1102 12-13 0.21998129745607997 1102 14-15 0.33218618246466036 1102 16-17 0.36545121627978006 1102 18-19 0.2576160571594741 1102 20-21 0.2856977730519077 1102 22-23 0.24976311939975204 1102 24-25 0.2992150389141912 1102 26-27 0.34331837243311725 1102 28-29 0.3363485518936855 1102 30-31 0.2716612869294721 1102 32-33 0.29516095756821414 1102 34-35 0.28632250813373616 1102 36-37 0.2902217577709507 1102 38-39 0.3283968718912931 1102 40-41 0.31499395435908895 1102 42-43 0.2762031977253585 1102 44-45 0.2832693182707402 1102 46-47 0.24767108832052998 1102 48-49 0.22908242444245985 1102 50-51 0.20257940726661872 1102 52-53 0.24539812094896973 1102 54-55 0.11973387036108818 1102 56-57 0.21144471554248412 1102 58-59 0.2363934947863271 1102 60-61 0.1895836356904823 1102 62-63 0.22134009469688465 1102 64-65 0.22317740379312312 1102 66-67 0.22553290421973315 1102 68-69 0.19487852241296366 1102 70-71 0.1921064054499695 1102 72-73 0.10558274706104953 1102 74-75 0.08774319232045258 1102 76-77 0.13743062989776433 1102 78-79 0.11352048315610119 1102 80-81 0.17789692512274513 1102 82-83 0.06801970042897665 1102 84-85 0.06473989316821616 1102 86-87 0.11171796552046942 1102 88-89 0.13846337222270932 1102 90-91 0.09837045249141596 1102 92-93 0.1610754632182534 1102 94-95 0.21694055865271622 1102 96-97 0.2173979389818257 1102 98-99 0.2102115617674727 1102 100 0.20590591538240233 1103 1 0.1422617259623209 1103 2 0.1614161339238862 1103 3 0.14425651274273577 1103 4 0.0987867315824218 1103 5 0.14797230703894826 1103 6 0.1537329573142614 1103 7 0.15321107559128677 1103 8 0.14630383006163328 1103 9 0.21743548171170346 1103 10-11 0.21059271143052172 1103 12-13 0.20917537413377119 1103 14-15 0.2609689715570127 1103 16-17 0.285559245722645 1103 18-19 0.2886449238228366 1103 20-21 0.30195312138189934 1103 22-23 0.22808198615152975 1103 24-25 0.218188055770149 1103 26-27 0.21155130073292128 1103 28-29 0.2767200780017589 1103 30-31 0.28952800525838285 1103 32-33 0.25867584294453394 1103 34-35 0.2172289652608015 1103 36-37 0.2992775456912895 1103 38-39 0.37829264408232177 1103 40-41 0.3530529607911177 1103 42-43 0.33947705028731434 1103 44-45 0.3888883368956755 1103 46-47 0.26231074224499906 1103 48-49 0.2906800409064161 1103 50-51 0.35015304998913876 1103 52-53 0.3057347404469297 1103 54-55 0.29491519753491957 1103 56-57 0.3205634283838208 1103 58-59 0.3108528574725824 1103 60-61 0.3952430923220689 1103 62-63 0.2833852391954892 1103 64-65 0.38368890229418895 1103 66-67 0.34467030618543504 1103 68-69 0.38501345543078713 1103 70-71 0.36506498762887674 1103 72-73 0.374143388503164 1103 74-75 0.40306345018577616 1103 76-77 0.4533790798959032 1103 78-79 0.3720791565127257 1103 80-81 0.3555844861689508 1103 82-83 0.4102031721241062 1103 84-85 0.45416923678480003 1103 86-87 0.39063152466854945 1103 88-89 0.3689275290422245 1103 90-91 0.3463565282865346 1103 92-93 0.38872808305909246 1103 94-95 0.33740247831254067 1103 96-97 0.3594582515768394 1103 98-99 0.4259600512041928 1103 100 0.4268794411729715 1104 1 0.13523312329774484 1104 2 0.17890266260479137 1104 3 0.17505456497352156 1104 4 0.10027837224305358 1104 5 0.11175275201691193 1104 6 0.1377096201439869 1104 7 0.07736591877274179 1104 8 0.13002537421499483 1104 9 0.11948875292982564 1104 10-11 0.1712356215790507 1104 12-13 0.1448607594174618 1104 14-15 0.21050438215861078 1104 16-17 0.20628989194139535 1104 18-19 0.24706870790338797 1104 20-21 0.28454496641457894 1104 22-23 0.25731140951865683 1104 24-25 0.2668333312622977 1104 26-27 0.23433445152061694 1104 28-29 0.2475626549996619 1104 30-31 0.21132452474503793 1104 32-33 0.22846182959523276 1104 34-35 0.22507668176390894 1104 36-37 0.2576574552710227 1104 38-39 0.27812835947776193 1104 40-41 0.3044496200850162 1104 42-43 0.3210203871176702 1104 44-45 0.2728931163528756 1104 46-47 0.33552601530048776 1104 48-49 0.33671469123218856 1104 50-51 0.37262081239144607 1104 52-53 0.4186479851334539 1104 54-55 0.3428218111679371 1104 56-57 0.3184034636164341 1104 58-59 0.29349518127304464 1104 60-61 0.3067662819816448 1104 62-63 0.37190038199452147 1104 64-65 0.40123842552153377 1104 66-67 0.334735364218929 1104 68-69 0.3567300586687807 1104 70-71 0.35377968089439094 1104 72-73 0.37043756342885814 1104 74-75 0.38104898065250836 1104 76-77 0.41039296843840845 1104 78-79 0.3298820933147866 1104 80-81 0.3337137864473263 1104 82-83 0.35301280648418043 1104 84-85 0.36525497457933653 1104 86-87 0.4083336387913903 1104 88-89 0.3605938678047238 1104 90-91 0.3985608109181271 1104 92-93 0.3832100101823528 1104 94-95 0.48079981853869924 1104 96-97 0.5096596955744133 1104 98-99 0.5069229607172403 1104 100 0.4555682845062776 1105 1 0.08577241372724842 1105 2 0.10150358662919245 1105 3 0.13623677816482171 1105 4 0.0392366825480579 1105 5 0.039050750152078706 1105 6 0.1359856466521734 1105 7 0.12864156924702996 1105 8 0.0912294477921165 1105 9 0.1533040372532284 1105 10-11 0.1523089788636085 1105 12-13 0.18421289162484555 1105 14-15 0.2777337069494692 1105 16-17 0.251291832050633 1105 18-19 0.22268750226432843 1105 20-21 0.21799485615184722 1105 22-23 0.22899308668977625 1105 24-25 0.21363498052263452 1105 26-27 0.28061989597664194 1105 28-29 0.19715467673452025 1105 30-31 0.27514944703048627 1105 32-33 0.3047031425158977 1105 34-35 0.3210623304206166 1105 36-37 0.26836701695089005 1105 38-39 0.29254858943139084 1105 40-41 0.20987154227932336 1105 42-43 0.16818367591399408 1105 44-45 0.18540705895983933 1105 46-47 0.31825873453327347 1105 48-49 0.30457445897264535 1105 50-51 0.36417908944751076 1105 52-53 0.3349689844492545 1105 54-55 0.35047021365853936 1105 56-57 0.3347326870209315 1105 58-59 0.41558896197565076 1105 60-61 0.40285285862936604 1105 62-63 0.40108470224493686 1105 64-65 0.4228983216388684 1105 66-67 0.3588668061352678 1105 68-69 0.42582606648633714 1105 70-71 0.4531720238711543 1105 72-73 0.41393404575782 1105 74-75 0.41850873506518127 1105 76-77 0.36002148291412794 1105 78-79 0.4299496842174051 1105 80-81 0.46650328979522726 1105 82-83 0.4791824913825806 1105 84-85 0.444295474646232 1105 86-87 0.3852061879821065 1105 88-89 0.428077319580801 1105 90-91 0.41022284194894354 1105 92-93 0.47012593460958385 1105 94-95 0.41960003770670085 1105 96-97 0.4567302363950745 1105 98-99 0.4099950236554051 1105 100 0.422719881522049 1106 1 0.07120295093129414 1106 2 0.06993490635603905 1106 3 0.066552310173428 1106 4 0.0339097975447018 1106 5 0.07989946668639902 1106 6 0.09425622849849447 1106 7 0.0789175902682473 1106 8 0.0699216512138392 1106 9 0.08111361481920198 1106 10-11 0.12636424293298631 1106 12-13 0.1335669896069689 1106 14-15 0.13563996670427514 1106 16-17 0.17180825433928248 1106 18-19 0.14205156351491155 1106 20-21 0.17346575955945553 1106 22-23 0.23677645187991203 1106 24-25 0.19012632787296013 1106 26-27 0.16355042249048068 1106 28-29 0.12243080465776046 1106 30-31 0.08226099715255941 1106 32-33 0.0657101168038281 1106 34-35 0.06551428651381741 1106 36-37 0.06875673896287537 1106 38-39 0.007322320611294231 1106 40-41 0.019159703094167924 1106 42-43 -0.09672894237309038 1106 44-45 -0.05767428492686122 1106 46-47 -0.06983678456155928 1106 48-49 -0.022467562074226066 1106 50-51 -0.07370194143006614 1106 52-53 -0.06499321499570243 1106 54-55 -0.02771997786712177 1106 56-57 -0.014723053403855602 1106 58-59 0.06312692288372546 1106 60-61 0.025242375389460392 1106 62-63 -0.04495757109069842 1106 64-65 0.03432764443593328 1106 66-67 -7.190554308849073E-4 1106 68-69 -0.026434569195849633 1106 70-71 -0.02951639094290215 1106 72-73 0.023713228940849262 1106 74-75 0.04498267876629569 1106 76-77 0.04723980677594142 1106 78-79 0.10344858740266005 1106 80-81 0.060507796784484924 1106 82-83 0.027646052759301654 1106 84-85 0.03022686920449047 1106 86-87 0.04484883590243527 1106 88-89 -0.0034259366547928494 1106 90-91 -0.04283472692802803 1106 92-93 0.10824428797635477 1106 94-95 0.09285251035046826 1106 96-97 0.08887516132847395 1106 98-99 0.08809879558635458 1106 100 0.05075643346146563 1107 1 0.0841536569976924 1107 2 0.07228337077054192 1107 3 0.10939798808431789 1107 4 0.07912410726779484 1107 5 0.0992428228567519 1107 6 0.07158906940428977 1107 7 0.07699338814610002 1107 8 0.06774407022264484 1107 9 0.08073624380132571 1107 10-11 0.11021339411792752 1107 12-13 0.11341930284067558 1107 14-15 0.16367638421090902 1107 16-17 0.13756372256732874 1107 18-19 0.12985269471503358 1107 20-21 0.058522423307515226 1107 22-23 0.1509124282765839 1107 24-25 0.21992000626520536 1107 26-27 0.17966774196943902 1107 28-29 0.1826153404963904 1107 30-31 0.11926630458088994 1107 32-33 0.06976036101192307 1107 34-35 0.09935285603947364 1107 36-37 0.10947815914603609 1107 38-39 0.14310683653150136 1107 40-41 0.15207638186404182 1107 42-43 0.14822464356450382 1107 44-45 0.24738507610690164 1107 46-47 0.13231569092425843 1107 48-49 0.10858583173363456 1107 50-51 0.12748150304526717 1107 52-53 0.14864060979692084 1107 54-55 0.20175849156848358 1107 56-57 0.1515156024349409 1107 58-59 0.16539571362829975 1107 60-61 0.20251035694833064 1107 62-63 0.17381823467153623 1107 64-65 0.07947294514179504 1107 66-67 0.11355738730573961 1107 68-69 0.2005498653908191 1107 70-71 0.16168116241148311 1107 72-73 0.1415516241803445 1107 74-75 0.1605630724040239 1107 76-77 0.19105065650393271 1107 78-79 0.21136756923955602 1107 80-81 0.2395511317915151 1107 82-83 0.23420136993935614 1107 84-85 0.20350170601870587 1107 86-87 0.22940255960908473 1107 88-89 0.2538703871522756 1107 90-91 0.2378856143408541 1107 92-93 0.34954496063791396 1107 94-95 0.27928350340806674 1107 96-97 0.31155535038745086 1107 98-99 0.263412863383671 1107 100 0.21122625913381654 1108 1 -0.030524150884957635 1108 2 -0.01521309114276903 1108 3 0.04573059025324966 1108 4 0.018235744933001286 1108 5 0.01485312653669979 1108 6 0.020619243879174576 1108 7 0.0440688896703918 1108 8 0.04868075466453092 1108 9 0.08143984286927974 1108 10-11 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-0.10229253912731906 2214 98-99 -0.09665943665250865 2214 100 -0.11641585782217234 2215 1 -0.1820518173727237 2215 2 -0.17567270563695558 2215 3 -0.19367920566808294 2215 4 -0.1249256338183784 2215 5 -0.1878501532183421 2215 6 -0.22940154886295971 2215 7 -0.22789061834821212 2215 8 -0.18265845701423444 2215 9 -0.2626948315819675 2215 10-11 -0.26314556028214753 2215 12-13 -0.29167764042163924 2215 14-15 -0.4028984499377728 2215 16-17 -0.38948129538295007 2215 18-19 -0.36623876441181835 2215 20-21 -0.37127094791491544 2215 22-23 -0.3712868997316221 2215 24-25 -0.42333289925467454 2215 26-27 -0.4398517002784885 2215 28-29 -0.46237078922036545 2215 30-31 -0.5284760633960701 2215 32-33 -0.45207488258369466 2215 34-35 -0.3821999899361259 2215 36-37 -0.42711218401649376 2215 38-39 -0.4396079508235573 2215 40-41 -0.36883202488011335 2215 42-43 -0.3753483139008935 2215 44-45 -0.4420877493059905 2215 46-47 -0.4026629999656848 2215 48-49 -0.40998563010948175 2215 50-51 -0.39274756006663125 2215 52-53 -0.2821314399688575 2215 54-55 -0.3483159528204567 2215 56-57 -0.31368360996418687 2215 58-59 -0.28248213022874324 2215 60-61 -0.3119355163968791 2215 62-63 -0.29309042068826585 2215 64-65 -0.29096274113557286 2215 66-67 -0.2917332432519899 2215 68-69 -0.23941955993665687 2215 70-71 -0.300789872183131 2215 72-73 -0.3397345773150491 2215 74-75 -0.3219777120652978 2215 76-77 -0.2632707097933249 2215 78-79 -0.3048585295657986 2215 80-81 -0.3382856259462912 2215 82-83 -0.2876520428013265 2215 84-85 -0.3055141183946759 2215 86-87 -0.2721205619258029 2215 88-89 -0.20176519058725262 2215 90-91 -0.18969967962328127 2215 92-93 -0.2471221166467501 2215 94-95 -0.3340762479089534 2215 96-97 -0.24092787366911494 2215 98-99 -0.2864970064326542 2215 100 -0.21438252648261624 2216 1 -0.42211148857447967 2216 2 -0.3553621617025229 2216 3 -0.4008913141792263 2216 4 -0.361870490348295 2216 5 -0.34549628424129963 2216 6 -0.2981753663308453 2216 7 -0.2914591988684876 2216 8 -0.33615876309109893 2216 9 -0.3846813531700377 2216 10-11 -0.4720150336644977 2216 12-13 -0.5050640914190083 2216 14-15 -0.6301737591857091 2216 16-17 -0.5961024825199956 2216 18-19 -0.6193151126666336 2216 20-21 -0.5964926468345411 2216 22-23 -0.6337866929229321 2216 24-25 -0.6888242491363812 2216 26-27 -0.6731345263330724 2216 28-29 -0.7122855840385682 2216 30-31 -0.6772433457092433 2216 32-33 -0.7154589897492016 2216 34-35 -0.6666812034343934 2216 36-37 -0.7279108791915121 2216 38-39 -0.783185162159981 2216 40-41 -0.7367058109130866 2216 42-43 -0.676269577695038 2216 44-45 -0.8297884226751009 2216 46-47 -0.7385647134446529 2216 48-49 -0.7124834655118164 2216 50-51 -0.8543521473592008 2216 52-53 -0.8559784125653351 2216 54-55 -0.7260415535307843 2216 56-57 -0.8029783647442272 2216 58-59 -0.7576916963716727 2216 60-61 -0.9002563401021177 2216 62-63 -0.976171125712888 2216 64-65 -1.0337258155811924 2216 66-67 -0.9603445090866387 2216 68-69 -0.9345671386204728 2216 70-71 -0.9581788710636019 2216 72-73 -0.9399015408670905 2216 74-75 -1.0147883525104646 2216 76-77 -0.9756062968818497 2216 78-79 -1.0779019814510704 2216 80-81 -1.067669832379977 2216 82-83 -0.9754910022502727 2216 84-85 -1.1122866894954981 2216 86-87 -1.0644807730361094 2216 88-89 -1.1609825161923695 2216 90-91 -1.1273439631552478 2216 92-93 -1.0518601038191022 2216 94-95 -1.0337470084686018 2216 96-97 -1.3508879182294926 2216 98-99 -1.3751903615314518 2216 100 -1.3837254814755298 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14.0 3 5.0 4 6.0 5 23.0 6 65.0 7 136.0 8 298.0 9 593.0 10 1050.0 11 1606.0 12 2564.0 13 3981.0 14 4753.0 15 6212.0 16 7655.0 17 10985.0 18 12705.0 19 6900.0 20 4768.0 21 4601.0 22 4726.0 23 5250.0 24 5984.0 25 7170.0 26 9190.0 27 11808.0 28 15388.0 29 19866.0 30 25211.0 31 32071.0 32 41007.0 33 53853.0 34 74949.0 35 114610.0 36 209774.0 37 454158.0 38 765251.0 39 341926.0 40 1111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.91572473227836 19.936461439947927 13.605822127409672 23.541991700364036 2 15.96396126527297 20.343796625401087 39.72059877262336 23.971643336702584 3 18.34266950995682 25.40987229949323 30.10899278921813 26.138465401331818 4 12.457397025682464 16.96378603515392 38.58682342498296 31.991993514180656 5 12.710086866037285 37.64816757351963 35.68204493022117 13.959700630221919 6 30.865109995884577 38.16744694704894 17.440957360045726 13.52648569702076 7 27.174730342676206 32.59700745682455 22.375026688350353 17.853235512148892 8 25.36126049744101 35.836908732933786 20.156368661198876 18.645462108426333 9 25.13540884342525 16.36204741972829 21.274869895673415 37.22767384117304 10-11 23.990186598297022 26.5138221984009 28.77373367886527 20.722257524436806 12-13 24.987434925734554 23.90102567253538 29.049567615571053 22.061971786159013 14-15 22.123950918965367 25.75649207989681 26.531793133995045 25.587763867142776 16-17 21.26458108950082 29.22289371124243 27.00182276741934 22.510702431837405 18-19 21.03328740473352 27.633059220486896 29.857966136609072 21.47568723817051 20-21 22.151787523900733 27.022199196674595 29.58697899387836 21.23903428554631 22-23 21.79244763825968 26.82149101800245 29.647476736894895 21.73858460684297 24-25 21.62733539770002 26.901062905717676 29.636569791728828 21.835031904853473 26-27 21.404568436817318 26.893009427031345 29.74269058066022 21.959731555491118 28-29 21.44929067370637 26.938043521023847 29.82697946306654 21.785686342203245 30-31 21.73385954233432 27.350402414620334 29.299860004712187 21.615878038333157 32-33 21.40133841800742 27.05758892911972 29.812224524284296 21.72884812858856 34-35 21.522060380431107 26.80109343773801 29.79365871534327 21.883187466487612 36-37 21.875893755832205 27.229499479052244 29.156099995446954 21.738506769668593 38-39 21.74328083482486 27.417345681659143 29.227931994325935 21.61144148919006 40-41 21.597147934329993 27.107266313621132 29.40677741333734 21.88880833871153 42-43 21.642185584710262 27.09529520299281 29.178555783404196 22.08396342889273 44-45 21.756586331232597 27.357669867205843 29.019353971734883 21.86638982982668 46-47 21.876247390288224 27.566778341480923 28.66315566590915 21.893818602321698 48-49 21.83986724562521 27.483939470158337 28.528000997249165 22.148192286967287 50-51 21.96825865531382 27.492426697102808 28.328330142519103 22.210984505064264 52-53 22.001743417868177 27.468246941172463 28.30974223142458 22.22026740953478 54-55 22.06190547969851 27.836115184046843 28.137831681492052 21.964147654762595 56-57 21.834142787868394 27.556301920721342 28.228030569930553 22.38152472147971 58-59 21.81113444607362 27.145002946217062 28.651883567623525 22.39197904008579 60-61 22.003710509529785 27.65034658386905 28.200115550058502 22.145827356542657 62-63 21.8053657840098 27.583598080295356 28.308482408675005 22.302553727019838 64-65 21.855139833694555 27.287871266448978 28.475464178376757 22.38152472147971 66-67 21.73793691822975 27.39040921041144 28.3310770713314 22.540576800027406 68-69 22.1688428887522 27.521935907520746 28.196694788803278 22.112526414923778 70-71 22.174309633252093 27.27624151746414 28.22057875411404 22.328870095169727 72-73 21.886661007018127 27.470240983001826 28.28103978691666 22.362058223063386 74-75 22.032094165991076 27.52634803542364 28.09948024460949 22.342077553975788 76-77 22.022784668001343 27.51145665126736 27.987028688153202 22.4787299925781 78-79 22.12874239188621 27.60886084174726 28.02259105313667 22.239805713229863 80-81 21.969805806058908 27.4291924359358 28.155822834442052 22.44517892356324 82-83 21.882398705696176 27.447529418005324 28.27331117221137 22.396760704087136 84-85 21.872600534960522 27.392768086965784 28.121896028817673 22.61273534925602 86-87 22.023677304722355 27.49773064684356 28.031876814184013 22.44671523425007 88-89 21.71740736721332 27.431076733130094 28.113208923372962 22.738306976283628 90-91 21.659744489094226 27.33444185426096 28.200246355993475 22.80556730065134 92-93 21.814770421847548 27.501548821757194 28.003382542728804 22.680298213666454 94-95 21.560339084796396 27.282008233552457 28.150098554199506 23.007554127451645 96-97 21.551005053891824 27.26402904761126 28.177822905583326 23.00714299291359 98-99 21.562995295993474 27.316413232043473 28.129530255781876 22.99106121618118 100 21.676554433404664 27.15545726482314 28.015496261862776 23.15249203990942 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 28.0 1 26.5 2 101.5 3 185.5 4 128.5 5 93.5 6 246.0 7 605.5 8 1043.5 9 1182.5 10 960.5 11 762.0 12 787.0 13 927.5 14 1104.0 15 1290.0 16 1491.5 17 1713.0 18 1999.0 19 2292.5 20 2701.0 21 3352.5 22 4260.0 23 5728.0 24 7762.0 25 10243.0 26 13309.0 27 17154.5 28 22165.0 29 28281.5 30 34465.0 31 40871.0 32 47239.5 33 53573.5 34 59749.5 35 64951.0 36 69565.5 37 73720.5 38 77004.5 39 78342.5 40 79603.5 41 81091.0 42 80774.0 43 80214.5 44 79180.5 45 77719.0 46 76710.0 47 74697.5 48 72532.5 49 70931.0 50 69618.0 51 67987.5 52 64448.5 53 61397.0 54 59485.0 55 56384.0 56 52374.0 57 47375.5 58 42806.5 59 39778.0 60 35911.0 61 30963.0 62 26583.5 63 22346.0 64 18153.5 65 14675.0 66 11665.5 67 8857.0 68 6786.0 69 5260.5 70 3835.5 71 2893.5 72 2296.5 73 1723.0 74 1291.5 75 888.0 76 560.5 77 404.0 78 280.5 79 173.5 80 87.5 81 37.0 82 21.5 83 15.5 84 8.0 85 3.5 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04062375813525015 2 0.24489186079356454 3 0.0024312368851346663 4 0.002519645499139563 5 1.7681722800979392E-4 6 8.840861400489696E-5 7 0.0 8 2.210215350122424E-4 9 0.0 10-11 4.6414522352570896E-4 12-13 0.0 14-15 2.210215350122424E-4 16-17 1.5471507450856965E-4 18-19 7.072689120391757E-4 20-21 6.630646050367271E-5 22-23 1.5471507450856965E-4 24-25 0.0012377205960685572 26-27 2.210215350122424E-4 28-29 2.6522584201469084E-4 30-31 1.7681722800979392E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.3261292100734542E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.210215350122424E-5 68-69 3.3153230251836355E-4 70-71 6.630646050367271E-5 72-73 4.1994091652326055E-4 74-75 2.210215350122424E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3261292100734542E-4 84-85 0.0 86-87 6.409624515355029E-4 88-89 0.0022765218106260967 90-91 0.0021881131966211996 92-93 8.619839865477453E-4 94-95 7.072689120391757E-4 96-97 2.6522584201469084E-4 98-99 1.105107675061212E-4 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2262223.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.9577809727943 #Duplication Level Percentage of deduplicated Percentage of total 1 78.9551787358734 52.86663564464437 2 13.257034760443851 17.753232596770395 3 3.759910035712602 7.552656979459667 4 1.5080014787285303 4.038897308774194 5 0.7544661054252593 2.525868811923082 6 0.4443233955457179 1.7850545160023057 7 0.2677571568907954 1.2549897545494397 8 0.19906178365661503 1.0662988248106715 9 0.15822845182619838 0.9535163418938631 >10 0.6598012027394218 7.581643006346585 >50 0.0287548658454279 1.275532410430715 >100 0.007019430010137795 0.7874692664226831 >500 1.9825598659523378E-4 0.06964835059689344 >1k 2.6434131545608115E-4 0.48855618737512946 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4402 0.1945873594247782 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3634 0.16063845164689777 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.420430700244848E-5 0.0 4 0.0 0.0 0.0 4.420430700244848E-5 0.0 5 0.0 0.0 0.0 4.420430700244848E-5 0.0 6 0.0 0.0 0.0 4.420430700244848E-5 0.0 7 0.0 0.0 0.0 4.420430700244848E-5 0.0 8 0.0 0.0 0.0 4.420430700244848E-5 0.0 9 0.0 0.0 0.0 8.840861400489696E-5 0.0 10-11 0.0 0.0 0.0 1.1051076750612118E-4 0.0 12-13 0.0 0.0 0.0 1.5471507450856965E-4 0.0 14-15 0.0 0.0 0.0 1.7681722800979392E-4 0.0 16-17 0.0 0.0 0.0 1.7681722800979392E-4 0.0 18-19 0.0 0.0 0.0 1.7681722800979392E-4 0.0 20-21 0.0 0.0 0.0 2.2102153501224236E-4 0.0 22-23 0.0 0.0 0.0 2.873279955159151E-4 0.0 24-25 0.0 0.0 0.0 3.094301490171393E-4 0.0 26-27 0.0 0.0 0.0 3.9783876302203626E-4 0.0 28-29 0.0 0.0 0.0 0.0010609033680587636 0.0 30-31 0.0 0.0 0.0 0.003624753174200775 0.0 32-33 0.0 0.0 0.0 0.008619839865477453 0.0 34-35 0.0 0.0 0.0 0.01637769574440716 0.0 36-37 0.0 0.0 0.0 0.03178289673476045 0.0 38-39 0.0 0.0 0.0 0.057266679721672 0.0 40-41 0.0 0.0 0.0 0.08683936110631003 0.0 42-43 0.0 0.0 0.0 0.11860015568756926 0.0 44-45 0.0 0.0 0.0 0.15577597787662845 0.0 46-47 0.0 0.0 0.0 0.20157163993116506 0.0 48-49 0.0 0.0 0.0 0.2528707382075065 0.0 50-51 0.0 0.0 0.0 0.31234763327930093 0.0 52-53 0.0 0.0 0.0 0.37182452835109536 0.0 54-55 0.0 0.0 0.0 0.4330253913959853 0.0 56-57 0.0 0.0 0.0 0.50622772379204 0.0 58-59 0.0 0.0 0.0 0.5912326061577484 0.0 60-61 0.0 0.0 0.0 0.6852993714589588 0.0 62-63 0.0 0.0 0.0 0.7833445243903895 0.0 64-65 0.0 0.0 0.0 0.8882855492142021 0.0 66-67 0.0 0.0 0.0 1.009604269782422 0.0 68-69 0.0 0.0 0.0 1.153400880461387 0.0 70-71 0.0 0.0 0.0 1.3076960140534333 0.0 72-73 0.0 0.0 0.0 1.4768216926448012 0.0 74-75 0.0 0.0 0.0 1.6385652519667602 0.0 76-77 0.0 0.0 0.0 1.8141447593804854 0.0 78-79 0.0 0.0 0.0 2.0148323131716017 0.0 80-81 0.0 0.0 0.0 2.2454903871103777 0.0 82-83 0.0 0.0 0.0 2.4878405002513015 0.0 84-85 0.0 0.0 0.0 2.732820769658871 0.0 86-87 0.0 0.0 0.0 2.993338852977801 0.0 88 0.0 0.0 0.0 3.2027346552483995 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2775 0.0 53.34561 1 GTATCAA 5480 0.0 44.47767 1 ATCAACG 6665 0.0 35.89408 3 TCAACGC 6795 0.0 35.34336 4 CAACGCA 6960 0.0 34.437195 5 TATCAAC 7200 0.0 33.505737 2 AACGCAG 7200 0.0 33.354557 6 ACGCAGA 8475 0.0 28.114805 7 CGCAGAG 8700 0.0 27.441717 8 GTACATG 6065 0.0 24.95205 1 GCAGAGT 9735 0.0 24.041424 9 TACATGG 6060 0.0 24.025232 2 GTGGTAT 1510 0.0 22.791761 1 ACATGGG 6200 0.0 22.43909 3 ATAACGC 215 4.950016E-8 21.860834 3 AGAGTAC 9015 0.0 21.34789 10-11 TGGTATC 1555 0.0 20.301407 2 GAGTACT 6905 0.0 20.146278 12-13 TAACGCC 120 0.005067066 19.582367 4 AGTACTT 7310 0.0 19.54443 12-13 >>END_MODULE