FastQCFastQC Report
Wed 25 May 2016
SRR1033008_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033008_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077039
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26950.036.8087231
ATCAACG26450.035.5493283
TCAACGC26650.035.282544
CAACGCA27000.034.8251725
GGTATCA16050.034.5647961
AACGCAG29150.032.248046
TATCAAC32450.029.7006682
GTACATG62700.026.1688691
ACGCAGA35500.025.9501347
TACATGG61900.025.8235112
CGCAGAG36700.024.738298
ACATGGG62600.024.182913
GCAGAGT44900.019.6984399
GAGTACA46150.019.6613181
CATGGGG46000.018.8055934
AATACCG1508.276923E-418.8055935
AGTACAT47350.017.5743942
ACCGTAC1350.00997636317.4125881
AGAGTAC38750.016.5776410-11
CATGGGA19700.016.2281764
Produced by FastQC (version 0.11.5)