##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033008_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2077039 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.237331605232256 33.0 31.0 34.0 28.0 34.0 2 31.33921943690032 33.0 31.0 34.0 28.0 34.0 3 31.51607408430944 34.0 31.0 34.0 28.0 34.0 4 34.86208925301836 37.0 35.0 37.0 32.0 37.0 5 34.963385376971736 37.0 35.0 37.0 32.0 37.0 6 34.98194400779186 37.0 35.0 37.0 32.0 37.0 7 34.872892131539174 37.0 35.0 37.0 32.0 37.0 8 34.88252218663203 37.0 35.0 37.0 32.0 37.0 9 36.47092086378734 39.0 37.0 39.0 32.0 39.0 10-11 36.45594521816875 39.0 37.0 39.0 32.0 39.0 12-13 36.382970180145875 39.0 37.0 39.0 32.0 39.0 14-15 37.72473843774719 40.0 38.0 41.0 32.0 41.0 16-17 37.720134287319596 40.0 38.0 41.0 32.0 41.0 18-19 37.718383959087916 40.0 38.0 41.0 32.0 41.0 20-21 37.734132098626944 40.0 38.0 41.0 32.0 41.0 22-23 37.69219066180269 40.0 38.0 41.0 32.0 41.0 24-25 37.693990339131815 40.0 38.0 41.0 32.0 41.0 26-27 37.60115144684332 40.0 38.0 41.0 32.0 41.0 28-29 37.60915129662948 40.0 38.0 41.0 32.0 41.0 30-31 37.76005818860406 40.0 38.0 41.0 32.0 41.0 32-33 37.74298316016213 40.0 38.0 41.0 32.0 41.0 34-35 37.691540938807606 40.0 38.0 41.0 32.0 41.0 36-37 37.60764386224813 40.0 38.0 41.0 31.0 41.0 38-39 37.50793220541357 40.0 38.0 41.0 31.0 41.0 40-41 37.42808199557158 40.0 38.0 41.0 31.0 41.0 42-43 37.29532666454506 40.0 38.0 41.0 30.5 41.0 44-45 37.15137631021854 40.0 37.5 41.0 30.0 41.0 46-47 37.005579336738506 40.0 37.0 41.0 30.0 41.0 48-49 36.875835745019714 40.0 37.0 41.0 30.0 41.0 50-51 36.73778080238262 40.0 36.5 41.0 30.0 41.0 52-53 36.5690379429563 40.0 36.0 41.0 30.0 41.0 54-55 36.39588086694569 40.0 36.0 41.0 30.0 41.0 56-57 36.21094428173953 39.5 35.0 41.0 29.5 41.0 58-59 35.998795882022435 39.0 35.0 41.0 29.0 41.0 60-61 35.76594734138358 39.0 35.0 41.0 29.0 41.0 62-63 35.504551671875205 38.5 35.0 40.5 28.5 41.0 64-65 35.22229938869708 38.0 35.0 40.0 28.0 41.0 66-67 34.92426285688425 37.5 34.0 40.0 28.0 41.0 68-69 34.60722957055693 37.0 34.0 40.0 28.0 41.0 70-71 34.27965796501655 36.5 34.0 39.5 27.0 41.0 72-73 33.9327918252859 36.0 34.0 39.0 26.5 41.0 74-75 33.587028698064884 35.5 34.0 39.0 26.0 40.5 76-77 33.22963844203214 35.0 33.0 37.5 26.0 40.0 78-79 32.85436286945021 35.0 33.0 37.0 26.0 39.0 80-81 32.51217526488429 35.0 33.0 37.0 25.5 39.0 82-83 32.295102306697174 35.0 33.0 36.0 25.5 38.5 84-85 32.08021154152618 35.0 33.0 36.0 25.0 37.0 86-87 31.905807979532405 35.0 33.0 36.0 25.0 37.0 88-89 31.686782000723145 35.0 33.0 35.0 25.0 37.0 90-91 31.473989414738963 35.0 33.0 35.0 24.5 36.0 92-93 31.248497981983007 35.0 33.0 35.0 24.0 36.0 94-95 31.034424486011098 35.0 32.0 35.0 23.0 36.0 96-97 30.850580802767787 35.0 32.0 35.0 20.5 35.5 98-99 30.657585148858544 35.0 32.0 35.0 19.5 35.0 100 30.5067223099807 35.0 32.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06141925186366848 1101 2 0.12509076574825073 1101 3 0.12734057591958958 1101 4 0.06333744395853813 1101 5 0.07685046738431822 1101 6 0.10307347738923767 1101 7 0.0743698044807104 1101 8 0.15061857219937735 1101 9 0.18841429896677653 1101 10-11 0.25906419288642013 1101 12-13 0.2208333060050407 1101 14-15 0.2659329251382161 1101 16-17 0.2936169739587058 1101 18-19 0.2451661908795657 1101 20-21 0.2864976459476978 1101 22-23 0.26039417802986975 1101 24-25 0.21212675132527892 1101 26-27 0.1245213971839263 1101 28-29 0.11589168264163874 1101 30-31 0.2012458368040484 1101 32-33 0.12196285829526943 1101 34-35 0.20066477138674088 1101 36-37 0.24686730874570628 1101 38-39 0.2557136302501064 1101 40-41 0.18219957829255407 1101 42-43 0.1881301944375977 1101 44-45 0.20342722609736796 1101 46-47 0.1580233819057213 1101 48-49 0.14697730972650902 1101 50-51 0.14148909739944315 1101 52-53 0.05451939871239375 1101 54-55 0.09127396338438842 1101 56-57 0.12934181432372327 1101 58-59 0.12864433057855962 1101 60-61 0.10466040168369517 1101 62-63 0.06940260065960757 1101 64-65 0.08754677749335826 1101 66-67 0.1483815660830814 1101 68-69 0.23887556325276194 1101 70-71 0.10389712979964116 1101 72-73 0.009238850724123893 1101 74-75 0.0994738204853931 1101 76-77 0.11883781073969857 1101 78-79 0.08460895661142587 1101 80-81 0.10430430053781947 1101 82-83 0.1755431990448102 1101 84-85 0.12317372996866993 1101 86-87 0.024723342939417137 1101 88-89 0.17546975667839604 1101 90-91 0.17870734374865194 1101 92-93 0.1711313616609047 1101 94-95 0.17977935086442187 1101 96-97 0.15099985354905243 1101 98-99 0.11204067699360465 1101 100 0.196246426985212 1102 1 0.02169445610066134 1102 2 0.07387738070213956 1102 3 0.004356643818802297 1102 4 -0.089112343282153 1102 5 -0.03970175673379117 1102 6 0.019206522939299475 1102 7 0.1470727527890645 1102 8 0.10791211389074107 1102 9 0.22587574179741665 1102 10-11 0.21484014184991196 1102 12-13 0.2148689472095029 1102 14-15 0.2869966022402437 1102 16-17 0.2787381792303236 1102 18-19 0.17407478753182914 1102 20-21 0.243645779904746 1102 22-23 0.14555537617123093 1102 24-25 0.19928332030919194 1102 26-27 0.12833737393339106 1102 28-29 0.1437903272485883 1102 30-31 0.2996542538214868 1102 32-33 0.2528137393329146 1102 34-35 0.26171063661559657 1102 36-37 0.2613066643023103 1102 38-39 0.28735979741249196 1102 40-41 0.23831409986205898 1102 42-43 0.24208404436579656 1102 44-45 0.21265992809006917 1102 46-47 0.207548383306964 1102 48-49 0.17492201160160903 1102 50-51 0.14926358723192834 1102 52-53 0.2108077893405067 1102 54-55 0.2664348077691656 1102 56-57 0.2404449237980586 1102 58-59 0.1907582420328282 1102 60-61 0.27990876636353335 1102 62-63 0.211081304238256 1102 64-65 0.20623555348642952 1102 66-67 0.2765152980127823 1102 68-69 0.3894559537448856 1102 70-71 0.22844551588865158 1102 72-73 0.1822023843939462 1102 74-75 0.2286504451698832 1102 76-77 0.2734607889289933 1102 78-79 0.18761402681602846 1102 80-81 0.23379138107210906 1102 82-83 0.13898142349922438 1102 84-85 0.11413616837138107 1102 86-87 0.08850484909707745 1102 88-89 0.1996633754611281 1102 90-91 0.2132207768505303 1102 92-93 0.1609442060567119 1102 94-95 0.1888846775195674 1102 96-97 0.2151901323184262 1102 98-99 0.17201459089201165 1102 100 0.0682568393734897 1103 1 -0.06845206501174061 1103 2 -0.014834033354045317 1103 3 0.021831725561234805 1103 4 -0.017229322855328633 1103 5 -0.020366375444901053 1103 6 0.051792528283321815 1103 7 0.11850531088771987 1103 8 0.0861910401643371 1103 9 0.09918947452603533 1103 10-11 0.12370732797655393 1103 12-13 0.16224945383679312 1103 14-15 0.18324677803041567 1103 16-17 0.18774863435108813 1103 18-19 0.21855629440522506 1103 20-21 0.21339712469960403 1103 22-23 0.18627310548951215 1103 24-25 0.18453408499645718 1103 26-27 0.14614052409335443 1103 28-29 0.059602543786930084 1103 30-31 0.10957200599249717 1103 32-33 0.14557942421144077 1103 34-35 0.060444507866868946 1103 36-37 0.14572956712549967 1103 38-39 0.19686960650436447 1103 40-41 0.14730785491602916 1103 42-43 0.19565005797195312 1103 44-45 0.14796837392912465 1103 46-47 0.16178591538866982 1103 48-49 0.13684102506713458 1103 50-51 0.1292436130730863 1103 52-53 0.09474056717688484 1103 54-55 0.21345483466506465 1103 56-57 0.20266585689231675 1103 58-59 0.23738405945715613 1103 60-61 0.19929227021334128 1103 62-63 0.1885831280613317 1103 64-65 0.23906052360209173 1103 66-67 0.20488200950594404 1103 68-69 0.19659655072833004 1103 70-71 0.29245862983584203 1103 72-73 0.26769804677132925 1103 74-75 0.2115134299112924 1103 76-77 0.12595316403007217 1103 78-79 0.18437344477475648 1103 80-81 0.23496269299420192 1103 82-83 0.18768936569940564 1103 84-85 0.256019826037587 1103 86-87 0.21778852610021104 1103 88-89 0.1792813834068454 1103 90-91 0.14238920141896472 1103 92-93 0.21003877677709681 1103 94-95 0.13580175729314803 1103 96-97 0.06895621711960587 1103 98-99 0.1422585876574729 1103 100 0.07179527589565637 1104 1 -0.10037065374625342 1104 2 -0.051058881071519124 1104 3 0.01977890576792518 1104 4 0.09774099060712871 1104 5 -0.01995615369416015 1104 6 0.015991162873696396 1104 7 0.0692098314544154 1104 8 0.1598384633386587 1104 9 0.14386386368488502 1104 10-11 0.16641765752277848 1104 12-13 0.20252946547186212 1104 14-15 0.2035401357745812 1104 16-17 0.16193011576920213 1104 18-19 0.28373407142254337 1104 20-21 0.2152818348494847 1104 22-23 0.24401834024415336 1104 24-25 0.13205393305614166 1104 26-27 0.1996960644396779 1104 28-29 0.10725692233734208 1104 30-31 0.12391287075192281 1104 32-33 0.09303275560559854 1104 34-35 0.09423155187480603 1104 36-37 0.11808665254059747 1104 38-39 0.1681027527640282 1104 40-41 0.14284824400643714 1104 42-43 0.17285195209281312 1104 44-45 0.1643171436916262 1104 46-47 0.13484196049167707 1104 48-49 0.10972703560713626 1104 50-51 0.053737359223248404 1104 52-53 0.09413143706943572 1104 54-55 0.05187858218405239 1104 56-57 0.03171417665140552 1104 58-59 0.04654132966453517 1104 60-61 -0.03647530216363748 1104 62-63 0.11968405373377067 1104 64-65 0.010156572811595765 1104 66-67 0.012983140503131096 1104 68-69 0.05911451664442069 1104 70-71 0.0158896064972609 1104 72-73 0.007761825773449971 1104 74-75 0.049479902089792915 1104 76-77 -0.015831493077577363 1104 78-79 0.013426683772770787 1104 80-81 -0.02287322686071036 1104 82-83 0.10561592787858842 1104 84-85 0.06372314032135051 1104 86-87 0.05743566695298696 1104 88-89 -0.01673018322103914 1104 90-91 0.044997681331356176 1104 92-93 0.15052903240641768 1104 94-95 0.13257559207516323 1104 96-97 0.14020433497601203 1104 98-99 0.14945864241074247 1104 100 0.23275662351046833 1105 1 0.03519972751304934 1105 2 -0.021479193960875165 1105 3 0.01639747265844349 1105 4 -0.004926903158747109 1105 5 0.015731493018527942 1105 6 0.12166974245401718 1105 7 0.01018416079025286 1105 8 0.07710234620722645 1105 9 0.13649666157578366 1105 10-11 0.16388896413215548 1105 12-13 0.15568374911605787 1105 14-15 0.12629968505643774 1105 16-17 0.1193003018652874 1105 18-19 0.17652413066253558 1105 20-21 0.22906476602513237 1105 22-23 0.1805885665455378 1105 24-25 0.09363337307171804 1105 26-27 0.10594008337804439 1105 28-29 0.018964830845156655 1105 30-31 0.13447639407623058 1105 32-33 0.13027542599125042 1105 34-35 0.15049980916359118 1105 36-37 0.1934579233421232 1105 38-39 0.21084218892660544 1105 40-41 0.2160978477120281 1105 42-43 0.12775103051200176 1105 44-45 0.05929216196200571 1105 46-47 0.0625537241012708 1105 48-49 0.10971478555531178 1105 50-51 0.0788614302609858 1105 52-53 0.13303194780236538 1105 54-55 0.07231549022909434 1105 56-57 0.060408038433124034 1105 58-59 0.09538553203957179 1105 60-61 0.03288621885305787 1105 62-63 0.07859313950297775 1105 64-65 0.12940266064761374 1105 66-67 0.09627349395378104 1105 68-69 0.10861875599996296 1105 70-71 -0.0020726863773958826 1105 72-73 0.15521685133103347 1105 74-75 0.07276782647032576 1105 76-77 0.01506638337070143 1105 78-79 0.033196173395829476 1105 80-81 0.09405006268654859 1105 82-83 0.09199905296104305 1105 84-85 0.07944262669866475 1105 86-87 0.03754205394155008 1105 88-89 -0.01374556802971938 1105 90-91 -0.021043080977850792 1105 92-93 -0.10799108804195612 1105 94-95 -0.041104124561247346 1105 96-97 -0.03841675940383027 1105 98-99 0.04023299226803445 1105 100 -0.04862329771227536 1106 1 -0.2678529626302364 1106 2 -0.18437648805909035 1106 3 -0.057790305838853584 1106 4 -0.07240449923239822 1106 5 -0.10070923424387956 1106 6 0.015822578629453687 1106 7 -0.03775421181772032 1106 8 -0.017049577900642987 1106 9 0.008599149594765265 1106 10-11 0.0122992303646825 1106 12-13 0.05695420856502409 1106 14-15 0.044203064672132086 1106 16-17 0.12315829699890202 1106 18-19 0.05667237204426101 1106 20-21 0.03287685375792648 1106 22-23 0.054343163619421375 1106 24-25 0.038380015903015874 1106 26-27 0.038496689838112275 1106 28-29 -0.01597161324662011 1106 30-31 0.1189116063700908 1106 32-33 0.12695808424258104 1106 34-35 0.06303344165508662 1106 36-37 0.05098020059242714 1106 38-39 0.10489890331466967 1106 40-41 0.04034810283759782 1106 42-43 0.03452039598658985 1106 44-45 0.025230948040608325 1106 46-47 -0.062423803567305924 1106 48-49 0.02999916822238191 1106 50-51 0.06447979686958405 1106 52-53 0.055402685026578524 1106 54-55 0.04420810136706166 1106 56-57 0.07024559919963025 1106 58-59 0.06157949435497301 1106 60-61 0.03721002716078203 1106 62-63 0.008885639571381887 1106 64-65 0.07742864241234315 1106 66-67 0.04694928534973286 1106 68-69 0.01762765271236333 1106 70-71 0.05707970896538228 1106 72-73 0.03246269103617294 1106 74-75 0.0616000658292819 1106 76-77 0.14989479812633277 1106 78-79 0.06083445550643063 1106 80-81 0.16999124040899005 1106 82-83 0.11185385180453977 1106 84-85 0.13783197618167264 1106 86-87 0.1596469234702802 1106 88-89 0.15849124727355957 1106 90-91 0.08473998476499389 1106 92-93 0.12821606885089665 1106 94-95 0.08567879018374214 1106 96-97 0.09681240770620292 1106 98-99 0.059103011492393165 1106 100 0.08053732650676793 1107 1 -0.0016767869111262712 1107 2 -0.019649947350391272 1107 3 -0.001116763563786094 1107 4 -0.016472847676851643 1107 5 0.062336388958222244 1107 6 0.030637165507293673 1107 7 0.07128943009741562 1107 8 0.12516870910008748 1107 9 0.11044424789479024 1107 10-11 0.05636599060314751 1107 12-13 0.05727373575610528 1107 14-15 0.1620665107217647 1107 16-17 0.09403754768331396 1107 18-19 0.1601904468209696 1107 20-21 0.2019763211133494 1107 22-23 0.1139384093248097 1107 24-25 0.01150776920698604 1107 26-27 -0.03371585976850611 1107 28-29 0.0034952260050928885 1107 30-31 -0.001577441957032022 1107 32-33 -0.0730444965921393 1107 34-35 -0.033169554892552355 1107 36-37 0.05894505359403013 1107 38-39 0.10720706292143234 1107 40-41 0.01313935499904062 1107 42-43 0.08976584329488446 1107 44-45 0.02582980095883869 1107 46-47 0.12906291605279563 1107 48-49 0.013038518010851874 1107 50-51 0.1243939741888056 1107 52-53 0.12511579546843166 1107 54-55 0.14201151323056393 1107 56-57 0.15253303271831697 1107 58-59 0.07151312719748404 1107 60-61 0.1580047058766354 1107 62-63 0.2000853902230446 1107 64-65 0.215724457688097 1107 66-67 0.295746715632454 1107 68-69 0.3259838992257542 1107 70-71 0.25601084633923676 1107 72-73 0.18551879639775848 1107 74-75 0.17330832033135835 1107 76-77 0.18490997541974963 1107 78-79 0.19011134532265572 1107 80-81 0.2869918935586995 1107 82-83 0.29581654484601216 1107 84-85 0.2738291610865673 1107 86-87 0.1963908629755231 1107 88-89 0.21202669305319688 1107 90-91 0.2154445724965015 1107 92-93 0.21856702266784822 1107 94-95 0.2400334524616703 1107 96-97 0.23227287825192988 1107 98-99 0.28387406167374607 1107 100 0.23778917428516522 1108 1 -0.28125848173848667 1108 2 -0.2415528709732051 1108 3 -0.18073927099408138 1108 4 -0.2119196016344702 1108 5 -0.13744610387556122 1108 6 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-0.16807643607798184 2214 66-67 -0.21924020464496863 2214 68-69 -0.19055409480981922 2214 70-71 -0.15583765869425292 2214 72-73 -0.23657255877640182 2214 74-75 -0.19319134122970638 2214 76-77 -0.1268327786653103 2214 78-79 -0.0707606242124541 2214 80-81 -0.16978307955470484 2214 82-83 -0.19400640759837273 2214 84-85 -0.19132715820588686 2214 86-87 -0.21587615727156972 2214 88-89 -0.19799971775243108 2214 90-91 -0.1580058349768052 2214 92-93 -0.19714704759459067 2214 94-95 -0.290467975804539 2214 96-97 -0.2628082663000981 2214 98-99 -0.27727081353475214 2214 100 -0.23292625152848245 2215 1 -0.22502381925015413 2215 2 -0.30367278551198496 2215 3 -0.3332965150360536 2215 4 -0.31196146176020534 2215 5 -0.2748909464392497 2215 6 -0.35876781654426537 2215 7 -0.2450007128975642 2215 8 -0.3476367747472011 2215 9 -0.2863702247642408 2215 10-11 -0.36505022480995564 2215 12-13 -0.3389849057164227 2215 14-15 -0.4995119348788535 2215 16-17 -0.5193049212684073 2215 18-19 -0.5077109875507162 2215 20-21 -0.47313752036554746 2215 22-23 -0.47571309292283814 2215 24-25 -0.40239747329371767 2215 26-27 -0.4178580446582103 2215 28-29 -0.38154995241361434 2215 30-31 -0.3943467282379558 2215 32-33 -0.47227858155782343 2215 34-35 -0.536216588735023 2215 36-37 -0.4210445815030113 2215 38-39 -0.4920229096894033 2215 40-41 -0.5092060315136706 2215 42-43 -0.4760738862978897 2215 44-45 -0.4059424294143881 2215 46-47 -0.40187287018965634 2215 48-49 -0.34883155103248953 2215 50-51 -0.3711968739202547 2215 52-53 -0.3625019925445372 2215 54-55 -0.3892030729825251 2215 56-57 -0.4411996942858778 2215 58-59 -0.31010752089196103 2215 60-61 -0.4258206065960053 2215 62-63 -0.5068190324598163 2215 64-65 -0.574124437591891 2215 66-67 -0.37478379582952215 2215 68-69 -0.29709193878814233 2215 70-71 -0.32853682558150865 2215 72-73 -0.5674313096833004 2215 74-75 -0.525651121237388 2215 76-77 -0.5450261990643739 2215 78-79 -0.4895829519466517 2215 80-81 -0.4773845452127574 2215 82-83 -0.43755446296392364 2215 84-85 -0.506221451831177 2215 86-87 -0.4388270785653674 2215 88-89 -0.4814430201166857 2215 90-91 -0.4472809105725375 2215 92-93 -0.4761073009382173 2215 94-95 -0.5962971242673554 2215 96-97 -0.43873462215267267 2215 98-99 -0.3806546668834194 2215 100 -0.3333248493754688 2216 1 -0.19999405200168496 2216 2 -0.1162570079253058 2216 3 -0.17058030873799623 2216 4 -0.24599558542185918 2216 5 -0.18338012470569254 2216 6 -0.337322640256005 2216 7 -0.2367467588934744 2216 8 -0.07048400418811696 2216 9 -0.1458356585313254 2216 10-11 -0.13581181749950133 2216 12-13 -0.2515404633719598 2216 14-15 -0.32449642125994416 2216 16-17 -0.2669582698476134 2216 18-19 -0.3393310503611815 2216 20-21 -0.25087697346716453 2216 22-23 -0.17979541905218355 2216 24-25 -0.24102032068065427 2216 26-27 -0.3336569330326 2216 28-29 -0.35568981280679424 2216 30-31 -0.40317378052436226 2216 32-33 -0.3977215071900986 2216 34-35 -0.49096737859084527 2216 36-37 -0.5329017900595971 2216 38-39 -0.48653518356069014 2216 40-41 -0.43267994815907684 2216 42-43 -0.5434263021699763 2216 44-45 -0.4343959814818774 2216 46-47 -0.3827162283132779 2216 48-49 -0.48937127534682645 2216 50-51 -0.39828262879562715 2216 52-53 -0.42768699048130543 2216 54-55 -0.4063208158375957 2216 56-57 -0.4562342022442891 2216 58-59 -0.3838921824115644 2216 60-61 -0.3540859083792043 2216 62-63 -0.4134256685087436 2216 64-65 -0.4877375988106749 2216 66-67 -0.5159987563131025 2216 68-69 -0.44213053383701606 2216 70-71 -0.419383475427324 2216 72-73 -0.41461456410980446 2216 74-75 -0.4786384772442318 2216 76-77 -0.3890545976493982 2216 78-79 -0.42004886450968826 2216 80-81 -0.46750071623111467 2216 82-83 -0.5850486591379642 2216 84-85 -0.30610872215873997 2216 86-87 -0.4116559594113234 2216 88-89 -0.5507156597628793 2216 90-91 -0.54557649934981 2216 92-93 -0.6609898305558417 2216 94-95 -0.5440696205897595 2216 96-97 -0.46872488873491847 2216 98-99 -0.4409761846665958 2216 100 -0.4414624118630641 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31753.0 3 6240.0 4 3539.0 5 2979.0 6 2400.0 7 2481.0 8 2951.0 9 3326.0 10 3794.0 11 4098.0 12 4542.0 13 4907.0 14 5366.0 15 5472.0 16 5531.0 17 5473.0 18 5278.0 19 5449.0 20 5207.0 21 5564.0 22 6381.0 23 9077.0 24 13937.0 25 17849.0 26 17725.0 27 15902.0 28 17400.0 29 21367.0 30 26275.0 31 31946.0 32 40378.0 33 54605.0 34 77626.0 35 120048.0 36 216181.0 37 421340.0 38 612670.0 39 239540.0 40 442.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.3324420870579 19.597831140144866 13.136825573468801 23.932901199328434 2 15.45355672397217 19.979924127845926 40.457169224959586 24.10934992322231 3 18.084171622859795 25.369462399571454 29.767796771834625 26.778569205734122 4 12.175563397518797 16.5826203935158 39.0002332031122 32.2415830058532 5 12.91513928691384 37.94784734025098 35.68177557866149 13.455237794173701 6 31.019052171801086 38.09166978291627 17.814522751192207 13.074755294090433 7 27.44683035010792 32.38056135044863 22.485727214504532 17.686881084938914 8 24.94010436371821 36.814357023778484 20.354057793806714 17.891480818696596 9 24.81530764548514 17.281151186758922 20.994034999616858 36.90950616813908 10-11 23.703042524396874 27.159089594651388 28.79385364574698 20.344014235204757 12-13 24.37981355727154 24.569186774818423 28.712720009601206 22.338279658308828 14-15 21.764930566015 26.564737777510018 25.69793656053258 25.972395095942403 16-17 21.587119948252372 29.770665022049354 26.204680172925727 22.437534856772544 18-19 21.51078548999357 28.769138199384514 28.07627495824579 21.64380135237613 20-21 22.713039941457524 28.059546777718104 27.56476936129281 21.66264391953156 22-23 22.419762614306542 27.776766108899892 27.60035032648 22.20312095031357 24-25 22.189136256445103 27.8432728475756 27.63959631643806 22.327994579541237 26-27 21.96422649718488 27.803750996354637 27.73619785040632 22.495824656054157 28-29 21.92505343569926 27.877283257504786 27.8010893583645 22.39657394843145 30-31 22.23742641059066 28.26235640775031 27.414757704528387 22.08545947713064 32-33 21.87935663618 27.981218960755193 27.955297464824696 22.18412693824011 34-35 22.074445159827004 27.637221424172044 27.942385325165453 22.3459480908355 36-37 22.434487798369005 28.03309005058502 27.36600942911329 22.166412721932684 38-39 22.128900669322885 28.251896880620958 27.602353495575027 22.01684895448113 40-41 21.952242588951854 27.878912725029874 27.884574611618675 22.284270074399597 42-43 21.92533904983412 27.827611652815087 27.881215893548966 22.36583340380183 44-45 22.054651127774264 28.01043153551962 27.796934381305142 22.13798295540097 46-47 22.053577666897155 28.152946193451083 27.700024815629508 22.093451324022254 48-49 21.982807012302803 27.978501296870313 27.71009274923496 22.328598941591927 50-51 22.017265864684475 27.87291629777654 27.700402195634027 22.409415641904953 52-53 22.029937031097056 27.840171688254177 27.8348232946153 22.295067986033466 54-55 22.186173335456438 28.236155111603935 27.568340651380474 22.009330901559157 56-57 21.984665077349142 27.983680265802757 27.636707645876413 22.394947010971684 58-59 21.884811204511482 27.629411842642522 28.02233579086454 22.46344116198146 60-61 22.173718133861808 28.05342697764634 27.545924648863938 22.226930239627915 62-63 21.920940947697797 28.052475410432397 27.6696083327371 22.356975309132704 64-65 21.981107554159863 27.784031684461997 27.786320188726926 22.44854057265122 66-67 21.81999249362464 27.849278920623693 27.632013197344925 22.698715388406747 68-69 22.236049712477335 28.00510027218957 27.551153884790025 22.20769613054307 70-71 22.19665153542553 27.798229777270862 27.624170321007934 22.380948366295673 72-73 21.914294702470105 27.88401828551828 27.811104564586813 22.3905824474248 74-75 22.032788622742952 27.94986799761047 27.667532229780512 22.34981114986607 76-77 22.026053331239382 27.94545189151848 27.569744760983493 22.458750016258644 78-79 22.122880484342357 28.014024848173335 27.581462767482044 22.281631900002264 80-81 22.048971542690783 27.89638067419324 27.668348408639215 22.386299374476764 82-83 21.840384701075077 27.886733328596424 27.850002227952007 22.422879742376487 84-85 21.908868946668985 27.83102910299989 27.68269874076939 22.577403209561734 86-87 22.137596693864577 27.91538624562891 27.59768127390253 22.349335786603987 88-89 21.887358644382925 27.86064271874823 27.643745913315936 22.60825272355291 90-91 21.87333680442 27.726051308183848 27.774577485106196 22.626034402289953 92-93 22.04020235561355 27.89079963990989 27.625042928077832 22.443955076398733 94-95 21.83668407059186 27.712628121146732 27.79862823674476 22.65205957151665 96-97 21.861286541426157 27.65688118336167 27.804177621779065 22.67765465343311 98-99 21.87455670780418 27.714778606179756 27.754420518808114 22.65624416720795 100 22.036001526076248 27.734123604468785 27.558875328048586 22.670999541406378 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 516.0 1 464.5 2 375.0 3 325.0 4 301.5 5 288.0 6 284.5 7 277.5 8 255.0 9 227.5 10 213.0 11 213.0 12 217.0 13 236.0 14 274.0 15 336.5 16 410.0 17 523.5 18 705.0 19 974.5 20 1433.0 21 2104.0 22 3147.5 23 4836.5 24 7086.0 25 9558.0 26 12739.5 27 17183.5 28 22708.0 29 28448.0 30 33962.5 31 39623.5 32 45373.0 33 50708.0 34 55891.5 35 60476.0 36 63861.5 37 67249.5 38 69512.5 39 70008.0 40 70974.5 41 72150.5 42 71720.5 43 71157.0 44 70732.5 45 69570.5 46 68821.5 47 67268.5 48 65413.5 49 64823.0 50 63533.5 51 61484.0 52 58349.5 53 55646.0 54 54005.5 55 51402.0 56 48158.0 57 44245.5 58 40387.5 59 37345.0 60 33886.5 61 29677.0 62 25829.5 63 21860.5 64 18038.5 65 14936.0 66 12072.5 67 9435.0 68 7367.5 69 5839.0 70 4441.5 71 3279.0 72 2557.5 73 1997.5 74 1521.0 75 1122.5 76 835.0 77 634.5 78 455.0 79 326.0 80 231.0 81 127.0 82 76.0 83 50.0 84 41.0 85 36.5 86 26.0 87 14.5 88 10.5 89 10.0 90 5.5 91 3.0 92 4.0 93 3.0 94 3.0 95 4.0 96 3.5 97 2.0 98 1.0 99 1.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0623002264281027 2 0.04405309674011899 3 0.057052371188022956 4 0.07679201016446971 5 0.08964684822961919 6 0.09123564843991856 7 0.09320961233756322 8 0.10495710480159498 9 0.10120175884997826 10-11 0.10510154118434946 12-13 0.1107345601117745 14-15 0.10719586873428953 16-17 0.0761420464420745 18-19 0.1010332497367647 20-21 0.09458175797373088 22-23 0.06071142621780332 24-25 0.022243202944191225 26-27 0.18567297003089495 28-29 0.06805360900782316 30-31 0.07643091920758349 32-33 0.074216228005348 34-35 0.021665457413173272 36-37 0.05430807991568767 38-39 0.07982517420231397 40-41 0.08468786575504841 42-43 0.0791270650190006 44-45 0.07710495566043778 46-47 0.08348422923209434 48-49 0.07982517420231397 50-51 0.07493840991912043 52-53 0.07941593778450959 54-55 0.07041273659281314 56-57 0.07294037329101669 58-59 0.06964240921812252 60-61 0.06660924518027828 62-63 0.0729163005605576 64-65 0.0696905546790407 66-67 0.07077382754969935 68-69 0.0704608820537313 70-71 0.06314277199417055 72-73 0.06249280827177535 74-75 0.06350386295105677 76-77 0.05873746232015865 78-79 0.05546357097772357 80-81 0.048963933753771595 82-83 0.05495804363808286 84-85 0.04477527865389143 86-87 0.04328276936542838 88-89 0.04508822414985949 90-91 0.04044218717125678 92-93 0.042945751139001245 94-95 0.042319860147065125 96-97 0.04590669698546826 98-99 0.04590669698546826 100 0.0542599344547695 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2077039.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.18001456507544 #Duplication Level Percentage of deduplicated Percentage of total 1 82.36586147957333 58.62803219781016 2 11.31446634700402 16.10727758751604 3 3.070982138126672 6.5577765996282915 4 1.206261411201252 3.4344681927437435 5 0.5887796268260502 2.095467120654897 6 0.33093525652653155 1.4133585827793294 7 0.2357308544445431 1.1745527956960178 8 0.15868135291003943 0.9035952809073988 9 0.11629416999562273 0.7450038642709608 >10 0.5824846121413049 7.20099761779194 >50 0.02424284559070087 1.1697484889588203 >100 0.00521232374695987 0.5264273372858944 >500 6.758191298672052E-5 0.043294333956573845 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.814546091816283E-5 0.0 0.0 0.0 3 0.0 4.814546091816283E-5 0.0 0.0 0.0 4 0.0 4.814546091816283E-5 0.0 0.0 0.0 5 0.0 4.814546091816283E-5 0.0 0.0 0.0 6 0.0 4.814546091816283E-5 0.0 0.0 0.0 7 0.0 4.814546091816283E-5 0.0 0.0 0.0 8 0.0 4.814546091816283E-5 0.0 0.0 0.0 9 0.0 4.814546091816283E-5 0.0 4.814546091816283E-5 0.0 10-11 0.0 4.814546091816283E-5 0.0 4.814546091816283E-5 0.0 12-13 0.0 4.814546091816283E-5 0.0 4.814546091816283E-5 0.0 14-15 0.0 4.814546091816283E-5 0.0 4.814546091816283E-5 0.0 16-17 0.0 4.814546091816283E-5 0.0 4.814546091816283E-5 0.0 18-19 0.0 7.221819137724424E-5 0.0 4.814546091816283E-5 0.0 20-21 0.0 9.629092183632566E-5 0.0 9.629092183632566E-5 0.0 22-23 0.0 9.629092183632566E-5 0.0 9.629092183632566E-5 0.0 24-25 0.0 9.629092183632566E-5 0.0 9.629092183632566E-5 0.0 26-27 0.0 9.629092183632566E-5 0.0 1.685091132135699E-4 0.0 28-29 0.0 9.629092183632566E-5 0.0 8.906910269860123E-4 0.0 30-31 0.0 9.629092183632566E-5 0.0 0.0031294549596805835 0.0 32-33 0.0 9.629092183632566E-5 0.0 0.00869025569572839 2.4072730459081415E-5 34-35 0.0 9.629092183632566E-5 0.0 0.01740458412191586 4.814546091816283E-5 36-37 0.0 9.629092183632566E-5 0.0 0.031077895022674107 4.814546091816283E-5 38-39 0.0 9.629092183632566E-5 0.0 0.058737462320158654 4.814546091816283E-5 40-41 0.0 9.629092183632566E-5 0.0 0.08916539362043756 4.814546091816283E-5 42-43 0.0 9.629092183632566E-5 0.0 0.1227949980717743 4.814546091816283E-5 44-45 0.0 9.629092183632566E-5 0.0 0.15875965737764192 4.814546091816283E-5 46-47 0.0 9.629092183632566E-5 0.0 0.20401639064071497 4.814546091816283E-5 48-49 0.0 9.629092183632566E-5 0.0 0.25743377952941665 4.814546091816283E-5 50-51 0.0 9.629092183632566E-5 0.0 0.3194692059224694 4.814546091816283E-5 52-53 0.0 9.629092183632566E-5 0.0 0.38160092323735856 4.814546091816283E-5 54-55 0.0 9.629092183632566E-5 0.0 0.4456584589889742 4.814546091816283E-5 56-57 0.0 9.629092183632566E-5 0.0 0.5230041419540028 4.814546091816283E-5 58-59 0.0 9.629092183632566E-5 0.0 0.6115436445825042 4.814546091816283E-5 60-61 0.0 9.629092183632566E-5 0.0 0.7098085303164745 4.814546091816283E-5 62-63 0.0 9.629092183632566E-5 0.0 0.8106732709400257 4.814546091816283E-5 64-65 0.0 9.629092183632566E-5 0.0 0.9159192485071297 4.814546091816283E-5 66-67 0.0 9.629092183632566E-5 0.0 1.0409289377811395 4.814546091816283E-5 68-69 0.0 9.629092183632566E-5 0.0 1.1825488110719153 4.814546091816283E-5 70-71 0.0 9.629092183632566E-5 0.0 1.3407788683794575 4.814546091816283E-5 72-73 0.0 9.629092183632566E-5 0.0 1.5071695813126282 4.814546091816283E-5 74-75 0.0 9.629092183632566E-5 0.0 1.6737047306285535 4.814546091816283E-5 76-77 0.0 9.629092183632566E-5 0.0 1.855694572899209 4.814546091816283E-5 78-79 0.0 9.629092183632566E-5 0.0 2.063345945839245 4.814546091816283E-5 80-81 0.0 9.629092183632566E-5 0.0 2.296297758491776 4.814546091816283E-5 82-83 0.0 9.629092183632566E-5 0.0 2.547472628101832 4.814546091816283E-5 84-85 0.0 9.629092183632566E-5 0.0 2.7919312059138033 4.814546091816283E-5 86-87 0.0 9.629092183632566E-5 0.0 3.049124258138629 4.814546091816283E-5 88 0.0 9.629092183632566E-5 0.0 3.2625290136583858 4.814546091816283E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2695 0.0 36.808723 1 ATCAACG 2645 0.0 35.549328 3 TCAACGC 2665 0.0 35.28254 4 CAACGCA 2700 0.0 34.825172 5 GGTATCA 1605 0.0 34.564796 1 AACGCAG 2915 0.0 32.24804 6 TATCAAC 3245 0.0 29.700668 2 GTACATG 6270 0.0 26.168869 1 ACGCAGA 3550 0.0 25.950134 7 TACATGG 6190 0.0 25.823511 2 CGCAGAG 3670 0.0 24.73829 8 ACATGGG 6260 0.0 24.18291 3 GCAGAGT 4490 0.0 19.698439 9 GAGTACA 4615 0.0 19.661318 1 CATGGGG 4600 0.0 18.805593 4 AATACCG 150 8.276923E-4 18.805593 5 AGTACAT 4735 0.0 17.574394 2 ACCGTAC 135 0.009976363 17.412588 1 AGAGTAC 3875 0.0 16.57764 10-11 CATGGGA 1970 0.0 16.228176 4 >>END_MODULE