FastQCFastQC Report
Wed 25 May 2016
SRR1033008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033008_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077039
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43450.20919202768941747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35260.16976089519744214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28450.056.3527531
GTATCAA54100.045.0621381
ATCAACG65650.036.7969743
TCAACGC66300.036.364464
CAACGCA67450.035.7435955
TATCAAC69150.035.035732
AACGCAG69500.034.5540476
ACGCAGA81250.029.3256327
CGCAGAG83850.028.3042188
GTACATG59800.025.7060221
GCAGAGT94800.024.787039
TAGACCG950.001305553624.7348465
TACATGG62550.024.5657772
ACATGGG61150.023.6721653
GTGGTAT13250.023.1321491
TAGAACG1303.1620442E-421.691084
GAGTACT66000.021.61165612-13
AGAGTAC86650.021.15236310-11
CAGAGTA91000.021.0966510-11
CATGGGG43800.020.7088744
Produced by FastQC (version 0.11.5)