FastQCFastQC Report
Wed 25 May 2016
SRR1033007_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033007_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2577007
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31800.036.6677321
GGTATCA22250.033.8103331
TCAACGC34350.033.3949174
ATCAACG35500.032.1806833
CAACGCA36500.031.3002345
AACGCAG38750.029.474216
TATCAAC42500.027.2121892
GTACATG90350.027.1124691
TACATGG91300.025.9523742
ACGCAGA47750.024.0172967
ACATGGG95750.023.518743
GCGTATA1000.001755463723.5087491
CGCAGAG51250.022.3970998
TATACTG13050.020.1749135
CATGGGG79250.019.6950054
GAGTACA64000.018.8071
GCAGAGT61000.018.2038149
GTATTAA12000.018.0233751
AGTACAT64600.017.538882
GTCGTAC2505.030666E-616.9262981
Produced by FastQC (version 0.11.5)