FastQCFastQC Report
Wed 25 May 2016
SRR1033007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033007_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2577007
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29650.048.1499021
GTATCAA51600.044.286311
TCAACGC61450.036.253134
ATCAACG63050.035.4849853
CAACGCA63200.035.247925
AACGCAG65050.034.245486
TATCAAC67350.033.2904852
ACGCAGA78450.028.396037
CGCAGAG82350.027.0512248
GCAGAGT92900.023.928629
GTACATG94750.023.7698351
TACATGG94550.022.81682
TAGACCG1050.002337077922.3796315
ACATGGG97600.020.9489483
GTGGTAT14850.020.9408821
CAGAGTA91650.019.38347210-11
ACACCGT4950.018.988786
AGAGTAC86400.018.79345110-11
GAGTACA66200.018.0069431
GAGTACT49350.017.5703912-13
Produced by FastQC (version 0.11.5)