##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033007_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2577007 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.673815010979794 34.0 31.0 34.0 31.0 34.0 2 32.67812660190678 34.0 31.0 34.0 31.0 34.0 3 32.787655602022035 34.0 31.0 34.0 31.0 34.0 4 36.282663570568495 37.0 37.0 37.0 35.0 37.0 5 36.2614637833735 37.0 37.0 37.0 35.0 37.0 6 36.327585450873826 37.0 37.0 37.0 35.0 37.0 7 36.28007102813458 37.0 37.0 37.0 35.0 37.0 8 36.28919944726576 37.0 37.0 37.0 35.0 37.0 9 38.08933929942759 39.0 39.0 39.0 35.0 39.0 10-11 38.047389859631735 39.0 38.5 39.0 35.0 39.0 12-13 38.02380920967619 39.0 38.0 39.0 35.0 39.0 14-15 39.515754904817875 41.0 39.0 41.0 37.0 41.0 16-17 39.49747206740223 41.0 39.0 41.0 36.0 41.0 18-19 39.49606694898384 41.0 39.5 41.0 36.5 41.0 20-21 39.4806577940999 41.0 39.0 41.0 36.5 41.0 22-23 39.415796697486655 41.0 39.0 41.0 36.0 41.0 24-25 39.359232435146666 41.0 39.0 41.0 36.0 41.0 26-27 39.24917375078919 41.0 39.0 41.0 36.0 41.0 28-29 39.2018017413224 41.0 39.0 41.0 36.0 41.0 30-31 39.226177887758936 41.0 39.0 41.0 36.0 41.0 32-33 39.2227089410312 41.0 39.0 41.0 35.5 41.0 34-35 39.155888788815865 41.0 39.0 41.0 35.0 41.0 36-37 39.08843243343925 41.0 39.0 41.0 35.0 41.0 38-39 39.00729897124843 41.0 39.0 41.0 35.0 41.0 40-41 38.916643416180094 41.0 39.0 41.0 35.0 41.0 42-43 38.809291554116854 41.0 38.5 41.0 35.0 41.0 44-45 38.66159405077285 40.5 38.0 41.0 35.0 41.0 46-47 38.50306712399306 40.0 38.0 41.0 35.0 41.0 48-49 38.32864598349946 40.0 38.0 41.0 34.0 41.0 50-51 38.179207507003284 40.0 38.0 41.0 34.0 41.0 52-53 38.021768276143604 40.0 37.0 41.0 34.0 41.0 54-55 37.84992143987191 40.0 37.0 41.0 33.5 41.0 56-57 37.66742814435506 40.0 36.5 41.0 33.0 41.0 58-59 37.45463691018301 39.5 36.0 41.0 33.0 41.0 60-61 37.22595592483839 39.0 35.5 41.0 33.0 41.0 62-63 36.96081558955797 39.0 35.0 41.0 33.0 41.0 64-65 36.66109230591923 38.5 35.0 41.0 32.0 41.0 66-67 36.34693522369167 37.5 35.0 40.0 32.0 41.0 68-69 36.01573433832349 37.0 35.0 40.0 32.0 41.0 70-71 35.66956531355949 36.5 35.0 39.0 31.0 41.0 72-73 35.31750068975366 36.0 35.0 39.0 31.0 41.0 74-75 34.97009088450284 35.5 35.0 38.5 31.0 40.0 76-77 34.62915079392489 35.0 34.5 37.0 31.0 39.5 78-79 34.293224077389 35.0 34.0 37.0 30.5 39.0 80-81 33.996967800242686 35.0 34.0 36.5 30.0 39.0 82-83 33.739789026572296 35.0 34.0 36.0 30.0 37.0 84-85 33.56331104261649 35.0 34.0 36.0 30.0 37.0 86-87 33.39905363082056 35.0 34.0 35.0 30.0 37.0 88-89 33.229232206198894 35.0 34.0 35.0 30.0 36.0 90-91 33.08068507380849 35.0 34.0 35.0 30.0 36.0 92-93 32.93092645848459 35.0 34.0 35.0 30.0 36.0 94-95 32.795547315160576 35.0 34.0 35.0 30.0 36.0 96-97 32.664910106957414 35.0 34.0 35.0 29.0 35.0 98-99 32.549564087330765 35.0 34.0 35.0 29.0 35.0 100 32.45226574859905 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1742919086669943 1101 2 0.15283706009356024 1101 3 0.2246882318486101 1101 4 0.11301334531334106 1101 5 0.12195515831154324 1101 6 0.12701255264405376 1101 7 0.19830482567384422 1101 8 0.19169559527988156 1101 9 0.27025026751191206 1101 10-11 0.2676162827694242 1101 12-13 0.24977861117128697 1101 14-15 0.3352797782091912 1101 16-17 0.3853305632966837 1101 18-19 0.28715449032655016 1101 20-21 0.33045691175031777 1101 22-23 0.30630193226155455 1101 24-25 0.3766784146769808 1101 26-27 0.35404825134474294 1101 28-29 0.37077153382947614 1101 30-31 0.4305407506760588 1101 32-33 0.37965897654973446 1101 34-35 0.40023918574883055 1101 36-37 0.358488775169036 1101 38-39 0.3627424883278323 1101 40-41 0.3917528817585776 1101 42-43 0.3543417185088984 1101 44-45 0.3881301737962488 1101 46-47 0.37215684704380436 1101 48-49 0.37161908302169167 1101 50-51 0.4101195202743497 1101 52-53 0.4056718486844417 1101 54-55 0.3728895747072656 1101 56-57 0.3448773974969157 1101 58-59 0.3418129543985913 1101 60-61 0.3977463768123002 1101 62-63 0.3186286510783063 1101 64-65 0.3225086391218994 1101 66-67 0.4438168548995307 1101 68-69 0.3509676008020577 1101 70-71 0.3616542985091371 1101 72-73 0.34024582643612433 1101 74-75 0.29590712880235515 1101 76-77 0.3800619354922077 1101 78-79 0.3904741306200279 1101 80-81 0.3681807582548373 1101 82-83 0.32568814788436384 1101 84-85 0.35624098951291927 1101 86-87 0.32297007767168395 1101 88-89 0.3873578534235378 1101 90-91 0.40418047475225194 1101 92-93 0.3955897097519454 1101 94-95 0.47457789326171707 1101 96-97 0.5025608988922201 1101 98-99 0.6128329192609527 1101 100 0.4984879084411702 1102 1 0.1929471155296696 1102 2 0.1304485826457622 1102 3 0.17095520895377092 1102 4 0.1350369749668232 1102 5 0.10993912449355747 1102 6 0.1293443915793162 1102 7 0.1689166206853585 1102 8 0.11416572795931046 1102 9 0.2235125137325653 1102 10-11 0.20669689770184618 1102 12-13 0.20989386971866253 1102 14-15 0.3307417205964356 1102 16-17 0.3128883370563713 1102 18-19 0.33362044529646084 1102 20-21 0.28709901579675545 1102 22-23 0.32902235597708085 1102 24-25 0.37064649565519403 1102 26-27 0.3229042111858931 1102 28-29 0.2935221757601454 1102 30-31 0.26183546909348365 1102 32-33 0.29152226802340664 1102 34-35 0.3022849424013856 1102 36-37 0.3039294987549326 1102 38-39 0.2630382718134996 1102 40-41 0.28061193772833093 1102 42-43 0.23239941383333473 1102 44-45 0.24711414823461553 1102 46-47 0.24164532531723637 1102 48-49 0.275312475470443 1102 50-51 0.23208680038730023 1102 52-53 0.279742225751626 1102 54-55 0.18819815552448205 1102 56-57 0.2375164549528037 1102 58-59 0.2822334086625773 1102 60-61 0.2677849752155197 1102 62-63 0.31047076503235616 1102 64-65 0.3107857054781249 1102 66-67 0.24559027608035677 1102 68-69 0.27676223252787935 1102 70-71 0.16930243794224964 1102 72-73 0.17516458402163693 1102 74-75 0.13284526402473773 1102 76-77 0.0744876242776229 1102 78-79 0.11971702397284645 1102 80-81 0.11881055209396152 1102 82-83 0.15532078190544496 1102 84-85 0.15502817401740998 1102 86-87 0.19439008609315067 1102 88-89 0.22468783468163878 1102 90-91 0.1795593170478469 1102 92-93 0.21503575042268608 1102 94-95 0.19668198097710388 1102 96-97 0.2236201464091252 1102 98-99 0.202920188805777 1102 100 0.23733623116510216 1103 1 0.10384814622019434 1103 2 0.0880027861724173 1103 3 0.10137117612406854 1103 4 0.0793287274582184 1103 5 0.16341501617778675 1103 6 0.12959148742916682 1103 7 0.12846870488686335 1103 8 0.13050961426188223 1103 9 0.20888139140305384 1103 10-11 0.219521632686984 1103 12-13 0.20514781170313512 1103 14-15 0.2333732556784085 1103 16-17 0.25280344498717255 1103 18-19 0.2284702470562081 1103 20-21 0.259859813837771 1103 22-23 0.22557329320298436 1103 24-25 0.24170476915926287 1103 26-27 0.22623055947753556 1103 28-29 0.25669719471103747 1103 30-31 0.23030344307420592 1103 32-33 0.18572682655686634 1103 34-35 0.2554848058755681 1103 36-37 0.2589721035404793 1103 38-39 0.25501182390564026 1103 40-41 0.20450788441976187 1103 42-43 0.21789172036553595 1103 44-45 0.2885132600230591 1103 46-47 0.27480006383459 1103 48-49 0.26794476461942196 1103 50-51 0.27583502932203885 1103 52-53 0.2847298233338478 1103 54-55 0.32930500681849395 1103 56-57 0.27781776425124605 1103 58-59 0.30331402636251426 1103 60-61 0.2603834504126681 1103 62-63 0.22605336116597385 1103 64-65 0.33177619232759525 1103 66-67 0.2858349906659967 1103 68-69 0.3034829538921855 1103 70-71 0.2905459738021108 1103 72-73 0.381299911266872 1103 74-75 0.2814709570718392 1103 76-77 0.3089345558202439 1103 78-79 0.324193959444564 1103 80-81 0.365140269874459 1103 82-83 0.3551229385370718 1103 84-85 0.38282193272390685 1103 86-87 0.3167886144718679 1103 88-89 0.34569669695328287 1103 90-91 0.38624519257916745 1103 92-93 0.41689063618154165 1103 94-95 0.4294408343190028 1103 96-97 0.43901918675964424 1103 98-99 0.47318657289575583 1103 100 0.324924050204892 1104 1 0.0794835173244266 1104 2 0.028789876610154863 1104 3 0.07381235181090773 1104 4 -0.004172751476950509 1104 5 0.07299021727109789 1104 6 0.08233324669583197 1104 7 0.07692646292012739 1104 8 0.08477218330740754 1104 9 0.0896697000113349 1104 10-11 0.11599491478101953 1104 12-13 0.13501472657204516 1104 14-15 0.1360160005211668 1104 16-17 0.17254675361448335 1104 18-19 0.21130462535145966 1104 20-21 0.21459069331882574 1104 22-23 0.21595534193742338 1104 24-25 0.21110097531940397 1104 26-27 0.19436751991364076 1104 28-29 0.2082334219607418 1104 30-31 0.18478524474842573 1104 32-33 0.22119081629378456 1104 34-35 0.13391035852527722 1104 36-37 0.12383976073206071 1104 38-39 0.13991191488774746 1104 40-41 0.20940336919452562 1104 42-43 0.14987227531835146 1104 44-45 0.08929970330770942 1104 46-47 0.15324468846675643 1104 48-49 0.1667642050984668 1104 50-51 0.17840319928728832 1104 52-53 0.16257910518424978 1104 54-55 0.12321624786779495 1104 56-57 0.20003780391792247 1104 58-59 0.18336526566069722 1104 60-61 0.18384518653409287 1104 62-63 0.1663659741798469 1104 64-65 0.12507141976384162 1104 66-67 0.14384762157174436 1104 68-69 0.20791580033797885 1104 70-71 0.12816768616920626 1104 72-73 0.07953858894276067 1104 74-75 0.11804717457619063 1104 76-77 0.19707265829122633 1104 78-79 0.18911635817461558 1104 80-81 0.19464207619296303 1104 82-83 0.20102638329494482 1104 84-85 0.25982192130356196 1104 86-87 0.28144465260060514 1104 88-89 0.29391927193739775 1104 90-91 0.2975590275703581 1104 92-93 0.2667914314184969 1104 94-95 0.30961004582005813 1104 96-97 0.44789572898258 1104 98-99 0.4303771290286704 1104 100 0.42969402534660617 1105 1 0.03851778355081592 1105 2 0.11678641209437757 1105 3 0.11831153079285173 1105 4 0.05055252324832793 1105 5 0.031852701256731564 1105 6 0.08860582469254297 1105 7 0.07745660601184312 1105 8 0.08606632430598182 1105 9 0.12896127871177754 1105 10-11 0.15661149032369792 1105 12-13 0.12758297933734042 1105 14-15 0.1824726309186815 1105 16-17 0.20726047995382402 1105 18-19 0.13215252949985512 1105 20-21 0.15201558369938084 1105 22-23 0.19715637407441733 1105 24-25 0.14499963823283935 1105 26-27 0.18469249871152726 1105 28-29 0.17878413561490447 1105 30-31 0.17987862427530388 1105 32-33 0.21484732398969442 1105 34-35 0.19212090003631488 1105 36-37 0.19838874212833701 1105 38-39 0.22512510891868232 1105 40-41 0.24520625268214502 1105 42-43 0.21124490925256367 1105 44-45 0.2048865958896755 1105 46-47 0.2067581044432245 1105 48-49 0.17951380361160574 1105 50-51 0.21624617732730655 1105 52-53 0.22566486999414792 1105 54-55 0.25134919866085426 1105 56-57 0.19910382820561523 1105 58-59 0.18918583261427102 1105 60-61 0.22898782714471366 1105 62-63 0.22252938384079357 1105 64-65 0.236806925637282 1105 66-67 0.24417729190141557 1105 68-69 0.30288936536080513 1105 70-71 0.2806080044715884 1105 72-73 0.2099492784174899 1105 74-75 0.24091983557693197 1105 76-77 0.18935941914941168 1105 78-79 0.1443862114740213 1105 80-81 0.14067051409331555 1105 82-83 0.17348113707974733 1105 84-85 0.1869046907508647 1105 86-87 0.13597143944267032 1105 88-89 0.09111445770400906 1105 90-91 0.1291552310384816 1105 92-93 0.16931872350244248 1105 94-95 0.15474582957653382 1105 96-97 0.1665063138000633 1105 98-99 0.17099983965138676 1105 100 0.21218883759510732 1106 1 0.08745181987931261 1106 2 0.07352337596756797 1106 3 0.0792523956099842 1106 4 0.07466259138766418 1106 5 0.08110584397644516 1106 6 0.09525098656418152 1106 7 0.10566903494240165 1106 8 0.08728231095397376 1106 9 0.0971969544458986 1106 10-11 0.14484236282739005 1106 12-13 0.07665875601554006 1106 14-15 0.1518838851717632 1106 16-17 0.17761422089404988 1106 18-19 0.21361833264780472 1106 20-21 0.15408558194582156 1106 22-23 0.1578431459223495 1106 24-25 0.10824671210902181 1106 26-27 0.11700673394783223 1106 28-29 0.15674711539213604 1106 30-31 0.12925957589713732 1106 32-33 0.15903666275883666 1106 34-35 0.10760410986955549 1106 36-37 0.140479284061648 1106 38-39 0.11263362546903011 1106 40-41 0.11968896971642096 1106 42-43 0.13056203959616397 1106 44-45 0.11480796984923813 1106 46-47 0.1659159519028819 1106 48-49 0.10295589002955552 1106 50-51 0.13318993655862243 1106 52-53 0.1659847318718164 1106 54-55 0.2064140422716818 1106 56-57 0.12244863707794451 1106 58-59 0.12460113183888666 1106 60-61 0.15646091900423897 1106 62-63 0.1384531670397422 1106 64-65 0.11283953354188725 1106 66-67 0.1474505929405865 1106 68-69 0.16983911996633339 1106 70-71 0.13805010059303413 1106 72-73 0.15362724295675179 1106 74-75 0.20782190443155457 1106 76-77 0.09892103600133595 1106 78-79 0.16144135297525253 1106 80-81 0.1774859584545112 1106 82-83 0.17297492649586843 1106 84-85 0.19349405752151938 1106 86-87 0.11327260614942247 1106 88-89 0.20086501548173885 1106 90-91 0.2015538797573697 1106 92-93 0.23502885651657124 1106 94-95 0.14688703762018207 1106 96-97 0.22820904166066924 1106 98-99 0.2858085872142482 1106 100 0.2789777221020202 1107 1 0.0548947979262806 1107 2 -0.006961609320264017 1107 3 0.04920028702572665 1107 4 0.03230091742034347 1107 5 0.054884380231598584 1107 6 0.044536718716003065 1107 7 0.060069500175757184 1107 8 0.07971634834613184 1107 9 0.08069419534918154 1107 10-11 0.06492431749933303 1107 12-13 0.06513592168960258 1107 14-15 0.111136052615322 1107 16-17 0.10942449860250036 1107 18-19 0.09267036513006133 1107 20-21 0.10465054618054381 1107 22-23 0.08846808838018916 1107 24-25 0.11957826032709562 1107 26-27 0.07702071968642343 1107 28-29 0.07222333564516248 1107 30-31 0.07106865220509917 1107 32-33 0.07744956215464782 1107 34-35 0.02159338081769846 1107 36-37 0.07030525528018217 1107 38-39 0.0033892345325128304 1107 40-41 -0.0010957510238185364 1107 42-43 0.04141968654353434 1107 44-45 0.137830987511812 1107 46-47 0.11876251270439298 1107 48-49 0.10647557437122401 1107 50-51 0.08465255850143905 1107 52-53 0.1688078899771881 1107 54-55 0.16416246342122776 1107 56-57 0.1499055942112264 1107 58-59 0.13382677001803955 1107 60-61 0.07759586195701473 1107 62-63 0.10904799199715143 1107 64-65 0.10430403541703015 1107 66-67 0.08079408651209974 1107 68-69 0.08169979662488913 1107 70-71 0.10257705480464097 1107 72-73 0.15783347512533652 1107 74-75 0.1384115565815378 1107 76-77 0.14388511239710056 1107 78-79 0.17990566778848205 1107 80-81 0.08694935552016858 1107 82-83 0.08071222504508313 1107 84-85 0.07411958537937124 1107 86-87 0.1028769994701122 1107 88-89 0.07111602436545184 1107 90-91 0.09010585689271267 1107 92-93 0.10767484995902521 1107 94-95 0.12159675903895106 1107 96-97 0.1477388576800891 1107 98-99 0.18298284137470944 1107 100 0.18877157082508234 1108 1 -0.0417964728452489 1108 2 -0.025578576240128825 1108 3 0.04433537286077893 1108 4 0.003947096562619379 1108 5 0.0011585342717523872 1108 6 0.06374294253603097 1108 7 0.07201341691898477 1108 8 0.0314548925175373 1108 9 0.022239476542118553 1108 10-11 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-0.24073918659404114 2214 76-77 -0.18357255685004503 2214 78-79 -0.16045795007629238 2214 80-81 -0.15014737332918315 2214 82-83 -0.14330273485538214 2214 84-85 -0.09306401834837175 2214 86-87 -0.16000061262204213 2214 88-89 -0.09891116084921237 2214 90-91 -0.06617287009668615 2214 92-93 -0.09027463427158011 2214 94-95 -0.15398621477006458 2214 96-97 -0.12766372179159902 2214 98-99 -0.12243034979107392 2214 100 -0.10808945614414966 2215 1 -0.21559566355698934 2215 2 -0.20018971522657836 2215 3 -0.21729903074980683 2215 4 -0.1861528406481412 2215 5 -0.1761214154110462 2215 6 -0.1806073669160142 2215 7 -0.18163289583834086 2215 8 -0.164035115584781 2215 9 -0.22226603623271757 2215 10-11 -0.2256527455657178 2215 12-13 -0.2643501395112011 2215 14-15 -0.3702407869308786 2215 16-17 -0.3815286260029467 2215 18-19 -0.4041959519636933 2215 20-21 -0.4113828170772109 2215 22-23 -0.42707074921730026 2215 24-25 -0.41619509573835245 2215 26-27 -0.44455881932663033 2215 28-29 -0.5482783148417099 2215 30-31 -0.5153406593968981 2215 32-33 -0.48674152295230755 2215 34-35 -0.4987923198098443 2215 36-37 -0.4693336403085553 2215 38-39 -0.4542229994456619 2215 40-41 -0.47836737997730694 2215 42-43 -0.5116374692378685 2215 44-45 -0.5439514098780549 2215 46-47 -0.5680052497299926 2215 48-49 -0.5194215595023692 2215 50-51 -0.4945181317077072 2215 52-53 -0.5012800939036808 2215 54-55 -0.5328268021012263 2215 56-57 -0.5012192598310534 2215 58-59 -0.5125813421401801 2215 60-61 -0.5299825437884778 2215 62-63 -0.4129397061940594 2215 64-65 -0.4211638456120852 2215 66-67 -0.4215411281992161 2215 68-69 -0.42348540315324357 2215 70-71 -0.38241803422413057 2215 72-73 -0.41626428629027856 2215 74-75 -0.36460033162595096 2215 76-77 -0.33838254816074453 2215 78-79 -0.3682834551068268 2215 80-81 -0.352276776854886 2215 82-83 -0.4195449696583893 2215 84-85 -0.4560714048169672 2215 86-87 -0.41929861520672773 2215 88-89 -0.36703991464446517 2215 90-91 -0.38299487505097574 2215 92-93 -0.41112991411844035 2215 94-95 -0.3572687587158683 2215 96-97 -0.3749257861134154 2215 98-99 -0.4384420651731631 2215 100 -0.477753020166773 2216 1 -0.2817386509193156 2216 2 -0.22662746538457412 2216 3 -0.23612575446172457 2216 4 -0.23050757948862355 2216 5 -0.25276855052926095 2216 6 -0.23225381079411278 2216 7 -0.2516942914696898 2216 8 -0.21813116130407906 2216 9 -0.3379564842310572 2216 10-11 -0.359718382124008 2216 12-13 -0.3886455586922608 2216 14-15 -0.426629250543229 2216 16-17 -0.380193609436148 2216 18-19 -0.4252967655735631 2216 20-21 -0.5021180335677542 2216 22-23 -0.5282208151485506 2216 24-25 -0.49224094202474333 2216 26-27 -0.48601791506928294 2216 28-29 -0.518298623341046 2216 30-31 -0.5223046896032315 2216 32-33 -0.4548845854278909 2216 34-35 -0.49992838531436234 2216 36-37 -0.48365142955066887 2216 38-39 -0.5713272924024935 2216 40-41 -0.49339129238192214 2216 42-43 -0.4958556244364516 2216 44-45 -0.5902863152159838 2216 46-47 -0.42967691328959745 2216 48-49 -0.46913384150168014 2216 50-51 -0.5310773032571063 2216 52-53 -0.4598420731037862 2216 54-55 -0.4663935164086723 2216 56-57 -0.4739293704808034 2216 58-59 -0.4764731428436306 2216 60-61 -0.5298149124328191 2216 62-63 -0.5662373313091962 2216 64-65 -0.5737603235928646 2216 66-67 -0.5384262957895984 2216 68-69 -0.5685070603588613 2216 70-71 -0.5791169146312996 2216 72-73 -0.5418566925741075 2216 74-75 -0.6271814194946757 2216 76-77 -0.5578963375228128 2216 78-79 -0.6077164427780772 2216 80-81 -0.6354496824401394 2216 82-83 -0.6232481388164075 2216 84-85 -0.7678367933303818 2216 86-87 -0.7800695726386806 2216 88-89 -0.8643845619197208 2216 90-91 -0.8117362380395718 2216 92-93 -0.8345071187578412 2216 94-95 -0.6763676644129717 2216 96-97 -0.8245787160303095 2216 98-99 -0.9483978366137897 2216 100 -0.9722333215348513 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16.0 3 6.0 4 14.0 5 26.0 6 54.0 7 130.0 8 290.0 9 522.0 10 805.0 11 1304.0 12 1901.0 13 2778.0 14 3396.0 15 4553.0 16 5395.0 17 7524.0 18 8426.0 19 5184.0 20 4118.0 21 4211.0 22 4611.0 23 5134.0 24 6071.0 25 7786.0 26 9937.0 27 13042.0 28 16899.0 29 21876.0 30 28229.0 31 35796.0 32 46044.0 33 60807.0 34 85754.0 35 133968.0 36 260939.0 37 584340.0 38 896355.0 39 308235.0 40 531.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.610193626731636 18.80674750881639 13.367643884584865 24.215414979867106 2 16.1001992111689 19.821958664752227 38.96984368478032 25.107998439298555 3 18.983018618982204 24.905469277515195 28.750882830023205 27.360629273479397 4 13.208268366451245 16.377901972639012 36.7114818502775 33.70234781063225 5 14.029242496031241 37.27861395582387 34.45653730321618 14.235606244928704 6 32.45677348054292 36.25959737773214 17.39425519381794 13.889373947906991 7 29.160029445011208 31.38058996347313 21.547632583070207 17.911748008445453 8 26.47021045830512 35.22051847209637 19.52883106098382 18.780440008614686 9 25.98925808117712 16.608181506685856 19.916011093489463 37.48654931864756 10-11 25.28500282499907 25.870433435571393 27.636329488023247 21.208234251406292 12-13 25.966828960883692 23.030185793053725 28.04196884214905 22.961016403913533 14-15 23.118329327704306 24.722113730372758 25.161190431426327 26.998366510496613 16-17 22.964205721062132 28.11769015492409 25.749746604972284 23.16835751904149 18-19 22.852711902849844 27.019785211527896 27.77849674456796 22.3490061410543 20-21 23.693455237024967 26.245660178649103 27.60138020579688 22.459504378529047 22-23 23.416712472451046 25.98628601008885 27.54094047602186 23.056061041438245 24-25 23.132880256099167 26.14717553033688 27.52126964588846 23.198674567675493 26-27 22.993871953632976 26.277821066633965 27.539751587502387 23.18855539223067 28-29 23.07448135923916 26.21893166105323 27.66992090222722 23.03666607748039 30-31 23.156648900564804 26.43345666772086 27.34616347271348 23.063730959000857 32-33 23.13348392146393 26.24465125628297 27.57540045486877 23.046464367384335 34-35 23.20608752711964 26.095777000217694 27.566087325335165 23.1320481473275 36-37 23.365167420965484 26.23687091265177 27.19119505690128 23.206766609481463 38-39 23.13720917327737 26.574724088836387 27.263818064910183 23.024248672976054 40-41 23.140682194499277 26.252528611680138 27.42351883405827 23.183270359762314 42-43 23.15511754527636 26.289722922754965 27.24957673766505 23.305582794303625 44-45 23.10661166228885 26.490440266557293 27.233938440989874 23.169009630163984 46-47 23.285831198751108 26.39305985587156 27.090768476763937 23.230340468613395 48-49 23.372928362243485 26.45450710844014 26.833473871044976 23.339090658271395 50-51 23.310239359070426 26.50241151847861 26.749325865238237 23.438023257212727 52-53 23.371919439877345 26.51925276105187 26.785899301010822 23.32292849805996 54-55 23.317573448578138 26.751324307617324 26.738285926270283 23.192816317534255 56-57 23.09421355859724 26.605787256301593 26.84267058645941 23.45732859864176 58-59 23.128342297867256 26.218749114767636 27.128292627843077 23.524615959522034 60-61 23.276712092749456 26.557067947428937 26.84911216772015 23.317107792101456 62-63 23.15084902757346 26.52290040345253 26.882969274045433 23.443281294928575 64-65 23.180010764425553 26.443855216536083 26.81896091085511 23.557173108183253 66-67 23.032824513088247 26.446396924804628 26.897734464826833 23.623044097280292 68-69 23.165060539245932 26.517381022783802 26.887733284284167 23.4298251536861 70-71 23.21101572483117 26.356544627158563 26.875926219835645 23.556513428174622 72-73 23.167512804602865 26.484680072161343 26.750783759273855 23.597023363961934 74-75 23.11916498480602 26.560832003948764 26.658018391102544 23.661984620142672 76-77 23.139304627422433 26.41123986081528 26.681863883179208 23.76759162858308 78-79 23.193126755185375 26.561142441599888 26.691274024478783 23.55445677873595 80-81 23.118893351861285 26.46108450617325 26.78161138095473 23.63841076101074 82-83 23.10366250460321 26.462384463837314 26.781650185661114 23.65230284589836 84-85 23.067942772371204 26.430681018716673 26.651421591016245 23.84995461789588 86-87 23.193167255367428 26.438914979709953 26.694560831819135 23.673356933103477 88-89 22.90126738482553 26.50452471128772 26.724085744443066 23.870122159443685 90-91 22.92695496007308 26.36697998182353 26.765317867035478 23.940747191067917 92-93 23.050099970993376 26.412229736350152 26.707068982410732 23.83060131024574 94-95 22.85836243694218 26.268218859138532 26.808246022506793 24.065172681412495 96-97 22.952988283110656 26.23150155034533 26.78829098801264 24.02721917853137 98-99 22.750539676454117 26.445504416557657 26.749733314655334 24.054222592332888 100 22.922172892817134 26.04552490544263 26.750451201723553 24.281851000016687 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10.0 1 11.0 2 60.5 3 104.0 4 67.0 5 54.5 6 134.5 7 253.5 8 445.0 9 557.0 10 466.0 11 375.0 12 393.5 13 470.0 14 564.0 15 684.0 16 801.5 17 935.5 18 1042.0 19 1178.5 20 1352.5 21 1577.5 22 2000.0 23 2704.0 24 3578.0 25 4643.0 26 6284.0 27 8544.0 28 11611.5 29 15626.5 30 19944.0 31 24852.0 32 30968.0 33 37902.5 34 44893.0 35 51664.0 36 58452.5 37 65894.5 38 72720.0 39 77696.0 40 82482.5 41 87541.0 42 92048.0 43 96193.0 44 98144.0 45 100074.0 46 102025.0 47 100255.0 48 98200.0 49 97137.5 50 97189.0 51 97092.0 52 92019.5 53 88141.5 54 86126.5 55 81710.0 56 75573.5 57 67987.5 58 60912.0 59 55410.0 60 50329.0 61 43696.5 62 37998.0 63 32073.0 64 25816.0 65 20911.0 66 16066.0 67 11569.5 68 8593.5 69 6721.0 70 4900.0 71 3422.5 72 2473.5 73 1722.0 74 1207.5 75 756.0 76 408.0 77 252.0 78 148.5 79 81.5 80 43.0 81 17.5 82 9.0 83 6.0 84 4.0 85 3.5 86 2.5 87 2.0 88 2.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043577685275981014 2 0.24710837029158242 3 0.0025999153281306567 4 0.00205664943867052 5 1.552188255600392E-4 6 3.88047063900098E-5 7 0.0 8 4.656564766801177E-4 9 0.0 10-11 5.820705958501472E-4 12-13 0.0 14-15 3.2984000431508337E-4 16-17 1.1641411917002942E-4 18-19 2.522305915350637E-4 20-21 0.0 22-23 1.3581647236503432E-4 24-25 0.001416371783235358 26-27 1.1641411917002942E-4 28-29 2.522305915350637E-4 30-31 7.76094127800196E-5 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.88047063900098E-5 70-71 0.0 72-73 1.552188255600392E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 1.7462117875504413E-4 88-89 0.0018238212003304608 90-91 0.0019014306131104805 92-93 3.6864471070509314E-4 94-95 2.7163294473006865E-4 96-97 1.94023531950049E-5 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2577007.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.183356387048775 #Duplication Level Percentage of deduplicated Percentage of total 1 77.81322048768877 43.718078982838236 2 12.16733206078622 13.672031069014338 3 3.845844143299998 6.482172963362043 4 1.8425607265720754 4.140849838631138 5 1.0267397700016092 2.8842843207378452 6 0.6577606723404154 2.2173121362891823 7 0.4513471554229246 1.7750738661184804 8 0.3362961688999887 1.511539800712577 9 0.25749135118461464 1.3020055515169147 >10 1.4686300970052115 15.03216273967023 >50 0.0965705136367352 3.706061424224353 >100 0.03579191558190622 3.2647664330995614 >500 2.0746878973633596E-4 0.08516864717511981 >1k 2.0746878972801146E-4 0.2084922266099997 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.88047063900098E-5 0.0 18-19 0.0 0.0 0.0 1.5521882556003924E-4 0.0 20-21 0.0 0.0 0.0 2.5223059153506376E-4 0.0 22-23 0.0 0.0 0.0 4.850588298751226E-4 0.0 24-25 0.0 0.0 0.0 9.507153065552403E-4 0.0 26-27 0.0 0.0 0.0 0.0014551764896253677 0.0 28-29 0.0 0.0 0.0 0.0018626259067204708 0.0 30-31 0.0 0.0 0.0 0.0044043341752661135 0.0 32-33 0.0 0.0 0.0 0.011098146027542805 0.0 34-35 0.0 0.0 0.0 0.018393430828864646 0.0 36-37 0.0 0.0 0.0 0.03379889926569854 0.0 38-39 0.0 0.0 0.0 0.060748767853560356 0.0 40-41 0.0 0.0 0.0 0.09210297061668828 0.0 42-43 0.0 0.0 0.0 0.12654214753782198 0.0 44-45 0.0 0.0 0.0 0.16571549863853688 0.0 46-47 0.0 0.0 0.0 0.2131736545535189 0.0 48-49 0.0 0.0 0.0 0.2678688882102377 0.0 50-51 0.0 0.0 0.0 0.33042207491093345 0.0 52-53 0.0 0.0 0.0 0.39328569926274937 0.0 54-55 0.0 0.0 0.0 0.4595447354236911 0.0 56-57 0.0 0.0 0.0 0.5401615129489366 0.0 58-59 0.0 0.0 0.0 0.6323614953315998 0.0 60-61 0.0 0.0 0.0 0.7339716190138404 0.0 62-63 0.0 0.0 0.0 0.8415964721865327 0.0 64-65 0.0 0.0 0.0 0.953916694832416 0.0 66-67 0.0 0.0 0.0 1.0820110306258384 0.0 68-69 0.0 0.0 0.0 1.2328837290701966 0.0 70-71 0.0 1.94023531950049E-5 0.0 1.3951650111932175 0.0 72-73 0.0 3.88047063900098E-5 0.0 1.5707175029016218 0.0 74-75 0.0 3.88047063900098E-5 0.0 1.7497430158319323 0.0 76-77 0.0 3.88047063900098E-5 0.0 1.9386831312448898 0.0 78-79 0.0 3.88047063900098E-5 0.0 2.1551939905479496 0.0 80-81 0.0 3.88047063900098E-5 0.0 2.390622144216139 0.0 82-83 0.0 3.88047063900098E-5 0.0 2.644657154598338 0.0 84-85 0.0 3.88047063900098E-5 0.0 2.8991966261636075 0.0 86-87 0.0 3.88047063900098E-5 0.0 3.165668544943805 0.0 88 0.0 3.88047063900098E-5 0.0 3.3822182089532546 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2965 0.0 48.149902 1 GTATCAA 5160 0.0 44.28631 1 TCAACGC 6145 0.0 36.25313 4 ATCAACG 6305 0.0 35.484985 3 CAACGCA 6320 0.0 35.24792 5 AACGCAG 6505 0.0 34.24548 6 TATCAAC 6735 0.0 33.290485 2 ACGCAGA 7845 0.0 28.39603 7 CGCAGAG 8235 0.0 27.051224 8 GCAGAGT 9290 0.0 23.92862 9 GTACATG 9475 0.0 23.769835 1 TACATGG 9455 0.0 22.8168 2 TAGACCG 105 0.0023370779 22.379631 5 ACATGGG 9760 0.0 20.948948 3 GTGGTAT 1485 0.0 20.940882 1 CAGAGTA 9165 0.0 19.383472 10-11 ACACCGT 495 0.0 18.98878 6 AGAGTAC 8640 0.0 18.793451 10-11 GAGTACA 6620 0.0 18.006943 1 GAGTACT 4935 0.0 17.57039 12-13 >>END_MODULE