FastQCFastQC Report
Wed 25 May 2016
SRR1033006_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033006_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2705883
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32200.035.6216241
GGTATCA20800.034.5786361
TCAACGC31750.034.3466034
ATCAACG33050.032.9956053
CAACGCA33750.032.311255
AACGCAG36100.030.2028566
TATCAAC38850.028.674572
GTACATG75350.026.0155221
TACATGG75600.025.180992
ACGCAGA43850.024.6504887
CGCAGAG46100.023.3614168
ACATGGG81950.022.5415173
ACCGATC2005.934071E-721.1629948
TATACCG2301.0089025E-720.4367455
GAGTACA56350.019.1038671
GCAGAGT55700.019.0001149
CATGGGG59100.018.5313914
AGTACAT58100.016.9086822
TATACTG17050.016.5411785
TAATACG3102.3032117E-615.1627454
Produced by FastQC (version 0.11.5)