##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033006_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2705883 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.585080360089478 33.0 31.0 34.0 30.0 34.0 2 31.732409716162895 34.0 31.0 34.0 30.0 34.0 3 31.841041168446676 34.0 31.0 34.0 30.0 34.0 4 35.20791105897779 37.0 35.0 37.0 33.0 37.0 5 35.20735486345862 37.0 35.0 37.0 33.0 37.0 6 35.325422791746725 37.0 35.0 37.0 33.0 37.0 7 35.24054218160948 37.0 35.0 37.0 33.0 37.0 8 35.240188877346135 37.0 36.0 37.0 33.0 37.0 9 36.87478542124696 39.0 38.0 39.0 34.0 39.0 10-11 36.865489934339365 39.0 38.0 39.0 34.0 39.0 12-13 36.78800136591272 39.0 38.0 39.0 33.5 39.0 14-15 38.18462771671946 41.0 39.0 41.0 34.0 41.0 16-17 38.18323722792154 41.0 39.0 41.0 33.5 41.0 18-19 38.18722224870773 41.0 39.0 41.0 33.5 41.0 20-21 38.20738461345151 41.0 39.0 41.0 34.0 41.0 22-23 38.171306556861474 40.0 39.0 41.0 34.0 41.0 24-25 38.1850924448692 40.0 38.5 41.0 33.5 41.0 26-27 38.09992228045337 40.0 38.0 41.0 33.0 41.0 28-29 38.112851331709464 40.0 38.0 41.0 33.0 41.0 30-31 38.264420893290655 40.5 39.0 41.0 33.5 41.0 32-33 38.253550874150875 41.0 39.0 41.0 33.5 41.0 34-35 38.207340080853456 41.0 38.5 41.0 33.0 41.0 36-37 38.12939011036323 40.5 38.0 41.0 33.0 41.0 38-39 38.03892407764859 40.0 38.0 41.0 33.0 41.0 40-41 37.964008052085035 40.0 38.0 41.0 33.0 41.0 42-43 37.83873009291237 40.0 38.0 41.0 33.0 41.0 44-45 37.69650092040195 40.0 38.0 41.0 32.0 41.0 46-47 37.55789625789437 40.0 38.0 41.0 32.0 41.0 48-49 37.43874605812594 40.0 37.5 41.0 32.0 41.0 50-51 37.30902721958044 40.0 37.0 41.0 31.0 41.0 52-53 37.15579923448279 40.0 37.0 41.0 31.0 41.0 54-55 36.99399105578475 40.0 36.5 41.0 31.0 41.0 56-57 36.81495134859859 40.0 36.0 41.0 30.5 41.0 58-59 36.617793710962374 39.5 35.5 41.0 30.0 41.0 60-61 36.39048750444864 39.0 35.0 41.0 30.0 41.0 62-63 36.140299857754385 39.0 35.0 41.0 30.0 41.0 64-65 35.86549843433733 38.5 35.0 40.5 30.0 41.0 66-67 35.56735102737258 38.0 35.0 40.0 29.5 41.0 68-69 35.253813080609916 37.0 35.0 40.0 29.0 41.0 70-71 34.92013217127274 37.0 34.0 39.5 29.0 41.0 72-73 34.571353048154705 36.0 34.0 39.0 29.0 41.0 74-75 34.21829066519136 36.0 34.0 39.0 28.5 40.5 76-77 33.86142545705043 35.0 34.0 38.5 28.0 40.0 78-79 33.478808026806774 35.0 34.0 37.0 28.0 39.0 80-81 33.132614011766215 35.0 33.5 37.0 27.0 39.0 82-83 32.913909618412916 35.0 33.5 36.5 27.0 39.0 84-85 32.690506019661605 35.0 33.5 36.0 27.0 37.0 86-87 32.5215284622432 35.0 34.0 36.0 27.0 37.0 88-89 32.30443537285241 35.0 33.0 35.0 27.0 37.0 90-91 32.09951649794171 35.0 33.0 35.0 27.0 36.0 92-93 31.889802330699446 35.0 33.0 35.0 26.0 36.0 94-95 31.694951703381115 35.0 33.0 35.0 26.0 36.0 96-97 31.52168275568456 35.0 33.0 35.0 25.0 36.0 98-99 31.348744199213343 35.0 33.0 35.0 25.0 35.0 100 31.21153612332832 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10076274992850998 1101 2 0.1930973718725788 1101 3 0.1744717813327199 1101 4 0.015523942596161078 1101 5 0.09824886956841539 1101 6 0.059294893470017485 1101 7 0.09265183689647927 1101 8 0.19975710087583565 1101 9 0.24861352857084285 1101 10-11 0.23678310949079417 1101 12-13 0.21186460834441334 1101 14-15 0.32281308120886365 1101 16-17 0.30811315958153074 1101 18-19 0.37381487374959477 1101 20-21 0.2968591519041155 1101 22-23 0.2808729971394186 1101 24-25 0.25462916566142013 1101 26-27 0.26967239937113874 1101 28-29 0.27969430293548925 1101 30-31 0.33462029644399394 1101 32-33 0.29634490112435685 1101 34-35 0.3529723057284002 1101 36-37 0.31804840388437583 1101 38-39 0.3377751752360112 1101 40-41 0.3289760859994715 1101 42-43 0.32167363431517515 1101 44-45 0.30798789985296793 1101 46-47 0.25964056056741214 1101 48-49 0.2522724265392853 1101 50-51 0.22799640424140932 1101 52-53 0.21278386350066114 1101 54-55 0.21426560680224327 1101 56-57 0.22332300275996886 1101 58-59 0.20858653780133096 1101 60-61 0.23739516851639308 1101 62-63 0.19188123435353077 1101 64-65 0.21986354818535858 1101 66-67 0.22338901429661462 1101 68-69 0.17244919201609576 1101 70-71 0.17847737361616112 1101 72-73 0.19118604630256186 1101 74-75 0.17812947184313543 1101 76-77 0.12100456363120315 1101 78-79 0.11747213538936307 1101 80-81 0.15741210141161588 1101 82-83 0.1237535586001286 1101 84-85 0.12317469428403172 1101 86-87 0.1427692553244455 1101 88-89 0.19601782775023224 1101 90-91 0.20802687462101233 1101 92-93 0.33556539259829066 1101 94-95 0.31142481946827516 1101 96-97 0.2534286610966241 1101 98-99 0.14256258761298213 1101 100 0.15942320261505927 1102 1 0.013582846807281612 1102 2 0.05700145749002772 1102 3 0.10748889495849667 1102 4 -0.05229194138699711 1102 5 0.06154423930497188 1102 6 0.07919317491759159 1102 7 0.14488602338574452 1102 8 0.1892473672449242 1102 9 0.1625142569442488 1102 10-11 0.207270743245779 1102 12-13 0.21788079709402552 1102 14-15 0.25019812085405846 1102 16-17 0.2557102938617888 1102 18-19 0.18128279572842843 1102 20-21 0.2303666682684593 1102 22-23 0.14907067850189293 1102 24-25 0.1947272788902623 1102 26-27 0.08117347071821968 1102 28-29 0.11159802222710624 1102 30-31 0.17238697451934826 1102 32-33 0.13204575998462076 1102 34-35 0.08171014388904041 1102 36-37 0.1930462127627095 1102 38-39 0.22487885703345256 1102 40-41 0.12059009147088773 1102 42-43 0.18156254518954995 1102 44-45 0.1926493722986109 1102 46-47 0.13146321132846595 1102 48-49 0.06450239864741292 1102 50-51 0.10828866867578313 1102 52-53 0.1810726880968403 1102 54-55 0.14154584398411174 1102 56-57 0.10826764982746795 1102 58-59 0.055436380753626224 1102 60-61 0.015442194295268052 1102 62-63 0.004642943410601674 1102 64-65 0.035513378421704545 1102 66-67 0.033902855352209826 1102 68-69 0.07426770415684558 1102 70-71 0.10208157712337851 1102 72-73 0.06623399101558647 1102 74-75 0.13162362013705575 1102 76-77 0.12743951658224972 1102 78-79 0.1177501478771319 1102 80-81 0.13942837750898462 1102 82-83 -0.042373535072329105 1102 84-85 0.02502135676751749 1102 86-87 0.006333902772233557 1102 88-89 0.029760158081650445 1102 90-91 0.05759374557449348 1102 92-93 0.10229475505467889 1102 94-95 0.1764003264418328 1102 96-97 0.09456524715378478 1102 98-99 0.10253294879302288 1102 100 0.005874224286813501 1103 1 0.04270822014423814 1103 2 0.1092424691200371 1103 3 0.10451591663466431 1103 4 0.05319243502715665 1103 5 0.008613827050467648 1103 6 0.12665886751420885 1103 7 0.16518070503114046 1103 8 0.1410070004696493 1103 9 0.19140219363535493 1103 10-11 0.20710846894493784 1103 12-13 0.29285201903718416 1103 14-15 0.3468734421500983 1103 16-17 0.316198516777888 1103 18-19 0.34064436125471786 1103 20-21 0.3210074360981281 1103 22-23 0.29316792429337823 1103 24-25 0.27716085602394 1103 26-27 0.1609412947294473 1103 28-29 0.2612640694938264 1103 30-31 0.21143688853871367 1103 32-33 0.1330694668811816 1103 34-35 0.12176248177028981 1103 36-37 0.14836803204499915 1103 38-39 0.20397552302132027 1103 40-41 0.23905089674738633 1103 42-43 0.24175421977548694 1103 44-45 0.2347734474411567 1103 46-47 0.19905275563111502 1103 48-49 0.1539508922893802 1103 50-51 0.0560016970041346 1103 52-53 0.1394635775438502 1103 54-55 0.16734498304646905 1103 56-57 0.21178790618550636 1103 58-59 0.16409900010831535 1103 60-61 0.08536099350786941 1103 62-63 0.07401425246571591 1103 64-65 0.1546703015595554 1103 66-67 0.12005509480826504 1103 68-69 0.13658909636428263 1103 70-71 0.1186213940751415 1103 72-73 0.09334157710934932 1103 74-75 0.07854868122856118 1103 76-77 0.0703645691888255 1103 78-79 0.12189348098419828 1103 80-81 0.19698668312488365 1103 82-83 0.19808127135812015 1103 84-85 0.15957991410301986 1103 86-87 0.057106172887166906 1103 88-89 0.10382801772959027 1103 90-91 0.1328883140874808 1103 92-93 0.18708982983520173 1103 94-95 0.21786731440454687 1103 96-97 0.1861188521180317 1103 98-99 0.2347179881631014 1103 100 0.19839122243143947 1104 1 -0.06297472978645402 1104 2 0.048773388622421976 1104 3 0.040819993751508576 1104 4 0.033505227626584144 1104 5 -0.07425304097203167 1104 6 0.04192424122168603 1104 7 0.09947149483109996 1104 8 0.1369128103848709 1104 9 0.15601939397627262 1104 10-11 0.1506316133479615 1104 12-13 0.17225674561466775 1104 14-15 0.1881990843952579 1104 16-17 0.17531440749289118 1104 18-19 0.15042597283309078 1104 20-21 0.10849004104095883 1104 22-23 0.02430036792119239 1104 24-25 -0.041036914427699855 1104 26-27 0.050612052198651725 1104 28-29 0.05610740896539568 1104 30-31 7.887422725403326E-4 1104 32-33 0.06838180554922246 1104 34-35 0.024468522580761487 1104 36-37 0.011942816224340902 1104 38-39 0.045556857362790026 1104 40-41 0.10399167563943479 1104 42-43 0.0671933475768185 1104 44-45 0.07572076836522257 1104 46-47 0.08907602130835102 1104 48-49 0.08399498460953936 1104 50-51 0.02737624188711152 1104 52-53 -0.004460313745681788 1104 54-55 -0.007862707499704413 1104 56-57 0.04720586858914544 1104 58-59 0.05533297531417247 1104 60-61 0.06381354819304619 1104 62-63 0.07422339965737024 1104 64-65 0.09721471569673668 1104 66-67 0.07259324875739992 1104 68-69 0.05078569878949679 1104 70-71 -7.660153587707441E-4 1104 72-73 0.026956822157764293 1104 74-75 0.011060805261585926 1104 76-77 0.03400974660824829 1104 78-79 0.03896048803164831 1104 80-81 0.006280487842239779 1104 82-83 0.04871650429267049 1104 84-85 -0.0159602981200635 1104 86-87 -0.03498641014500947 1104 88-89 -0.04545829373238064 1104 90-91 0.009708612814975481 1104 92-93 -0.042318690795642766 1104 94-95 -0.003524303718563715 1104 96-97 -0.0018422100543062925 1104 98-99 0.012864647295863563 1104 100 0.1248077254795561 1105 1 -0.13139435913861774 1105 2 -0.12724836426263053 1105 3 -0.07615623618833922 1105 4 -0.04795215874072767 1105 5 -0.20102246841259586 1105 6 -0.09847877975357022 1105 7 -0.04677240609312605 1105 8 -0.05947451828787109 1105 9 -0.012674928303354704 1105 10-11 -0.008902029742735351 1105 12-13 0.08257830325166537 1105 14-15 0.0442152217960583 1105 16-17 0.07870123025449516 1105 18-19 0.038595933097710144 1105 20-21 -0.021037637215087557 1105 22-23 -0.061831779690997735 1105 24-25 -0.14861322484753003 1105 26-27 -0.065017302551162 1105 28-29 -0.0652234090450392 1105 30-31 -0.04478634671477266 1105 32-33 -0.06918617221656831 1105 34-35 -0.10657016085990278 1105 36-37 -0.08961768026727412 1105 38-39 -0.06808158826081723 1105 40-41 -0.016836854125863 1105 42-43 -0.026560267229427836 1105 44-45 -0.09072237873846234 1105 46-47 -0.07236397517226578 1105 48-49 -0.07269248781442883 1105 50-51 -0.1272576324217951 1105 52-53 -0.08698147208810525 1105 54-55 -0.08269349898538536 1105 56-57 -0.015453425490136397 1105 58-59 -0.14165258357530774 1105 60-61 -0.1317891653538581 1105 62-63 -0.17593937175230678 1105 64-65 -0.05845207130173691 1105 66-67 -0.1361326185696683 1105 68-69 -0.011994962207346305 1105 70-71 -0.09076624825903679 1105 72-73 -0.08807061478771772 1105 74-75 -0.13944413545882384 1105 76-77 -0.16281582273586537 1105 78-79 -0.14870032636883934 1105 80-81 -0.03634369005215632 1105 82-83 -0.07913144547333673 1105 84-85 -0.09428012970728616 1105 86-87 -0.17810685969428874 1105 88-89 -0.16212722470721275 1105 90-91 -0.15266020287421966 1105 92-93 -0.09331097532731292 1105 94-95 -0.06674579316753082 1105 96-97 -0.018271059669309864 1105 98-99 -0.037598630154345614 1105 100 -0.013510579317024707 1106 1 -0.19848421894975132 1106 2 -0.13005447356134425 1106 3 -0.03483770970562716 1106 4 -0.06921933255184598 1106 5 -0.07122208596774726 1106 6 -0.05264517026461846 1106 7 -0.025490013038997006 1106 8 -0.020950809407445092 1106 9 0.04994317424979755 1106 10-11 0.08476431044663002 1106 12-13 0.0255054055323356 1106 14-15 0.055840514214182235 1106 16-17 0.0437936257878917 1106 18-19 0.09041383485504184 1106 20-21 0.10820245616233848 1106 22-23 0.0715429156173002 1106 24-25 0.02668913487155322 1106 26-27 -0.10360802519517875 1106 28-29 -0.09502293233907011 1106 30-31 -0.02973273570389523 1106 32-33 -0.025500562501775903 1106 34-35 0.013542130151662946 1106 36-37 -0.05504809027858926 1106 38-39 0.06880009930775799 1106 40-41 0.10551100867511565 1106 42-43 0.04066392694847565 1106 44-45 -0.0017846205329590248 1106 46-47 -0.09000080721318682 1106 48-49 -0.1034848212434909 1106 50-51 -0.07347933260761863 1106 52-53 -0.029303854930610385 1106 54-55 -0.1368136180882331 1106 56-57 -0.10793113052648096 1106 58-59 -0.15112705329514853 1106 60-61 -0.15139026163029712 1106 62-63 -0.11425473656001373 1106 64-65 -0.04780706996391615 1106 66-67 -0.07560789834599291 1106 68-69 -0.03209221807963303 1106 70-71 -0.06670499326062185 1106 72-73 -0.009861563756608405 1106 74-75 0.015835346301408038 1106 76-77 -0.0191416274533438 1106 78-79 -0.16554446904677889 1106 80-81 -0.10933849341708424 1106 82-83 -0.08841831347585583 1106 84-85 -0.13608886309806678 1106 86-87 -0.15206783951897052 1106 88-89 -0.16762321247290402 1106 90-91 -0.14035519942534336 1106 92-93 -0.12895922916464997 1106 94-95 -0.09580681496787236 1106 96-97 -0.11232536781970381 1106 98-99 -0.1824205281903275 1106 100 -0.12073186465573826 1107 1 -0.12178640812765806 1107 2 -0.08548973526546177 1107 3 -0.04353666717973326 1107 4 -0.15923502875651252 1107 5 -0.14383178726208712 1107 6 -0.0852218210008786 1107 7 -0.06374453512445655 1107 8 -0.0901034579253448 1107 9 -0.0012057963630311974 1107 10-11 -0.00839987766238437 1107 12-13 -0.0024732783565895033 1107 14-15 -0.006837625530287994 1107 16-17 -0.050006478759790696 1107 18-19 -0.03271559536476332 1107 20-21 -0.019532259711063205 1107 22-23 -0.009253778015356318 1107 24-25 -0.1069694813424249 1107 26-27 -0.1325358118804374 1107 28-29 -0.12719019832814382 1107 30-31 -0.09917608720660809 1107 32-33 -0.11365458859467026 1107 34-35 -0.17039381612432436 1107 36-37 -0.07033040181288897 1107 38-39 -0.13596938522812962 1107 40-41 -0.1918793743431948 1107 42-43 -0.19961863049771011 1107 44-45 -0.1674835068097167 1107 46-47 -0.14936961696685103 1107 48-49 -0.14969201676593258 1107 50-51 -0.07202072379440949 1107 52-53 -0.0980302561716968 1107 54-55 -0.10965581150988157 1107 56-57 2.9451681901093707E-4 1107 58-59 -0.07801662855144542 1107 60-61 -0.041218046020368604 1107 62-63 -0.06261517172569597 1107 64-65 -0.10802866702346847 1107 66-67 -0.10096129715278579 1107 68-69 0.009841731391794895 1107 70-71 -0.005476445560042009 1107 72-73 -0.12015872602707844 1107 74-75 -0.11373621429292058 1107 76-77 -0.13860375023035942 1107 78-79 -0.08877522667555127 1107 80-81 -0.022734883243167303 1107 82-83 -0.07033003017036776 1107 84-85 -0.11075244392181105 1107 86-87 -0.13653323409513263 1107 88-89 -0.1298321757505292 1107 90-91 -0.05408755173417035 1107 92-93 -0.07223227427051171 1107 94-95 -0.030128784361444616 1107 96-97 -0.02382835319005494 1107 98-99 0.008289763714113718 1107 100 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0.08146127398303804 2214 6 0.09025075333024546 2214 7 0.012649275865072696 2214 8 -0.04270351589201482 2214 9 -0.04393590011614634 2214 10-11 -0.07936563368650695 2214 12-13 -0.007656645353335989 2214 14-15 -0.11692772367333504 2214 16-17 -0.13392008073222428 2214 18-19 -0.062324871115464475 2214 20-21 -0.13603714037827785 2214 22-23 -0.07035970743988429 2214 24-25 -0.11418243421455543 2214 26-27 -0.10147518735126226 2214 28-29 -0.17265498357608067 2214 30-31 -0.15604963709932207 2214 32-33 -0.0814889556067584 2214 34-35 -0.10441775340956383 2214 36-37 -0.08716827481531197 2214 38-39 -0.09504381762277347 2214 40-41 -0.1253553971795327 2214 42-43 -0.10792888701678294 2214 44-45 -0.049206143215734244 2214 46-47 -0.09829333601932433 2214 48-49 -0.07834041821310933 2214 50-51 0.018807420077294523 2214 52-53 -0.04440476600571941 2214 54-55 -0.0933622413258206 2214 56-57 -0.1049138607191793 2214 58-59 -0.04231724081311228 2214 60-61 -0.0381177621763058 2214 62-63 0.016607524121738493 2214 64-65 0.009370016958563099 2214 66-67 -0.0855049480718364 2214 68-69 -0.05919887507320709 2214 70-71 -0.0523375774631063 2214 72-73 0.026303426041231148 2214 74-75 -0.032597172235789174 2214 76-77 0.04848555847044622 2214 78-79 0.07890171839063953 2214 80-81 0.09221172933330735 2214 82-83 0.17537265619051823 2214 84-85 0.12851529497777392 2214 86-87 0.1512324577623545 2214 88-89 0.14445807113150266 2214 90-91 0.10377493784100267 2214 92-93 0.09883569089772237 2214 94-95 0.1581148165064299 2214 96-97 0.060924727154578306 2214 98-99 0.02123181600510904 2214 100 0.054115534425481115 2215 1 -0.08848090607686387 2215 2 -0.12178827450478025 2215 3 -0.11002128555207946 2215 4 -0.1227011860050169 2215 5 -0.09288718237676363 2215 6 -0.11181537477088455 2215 7 -0.12864221259219022 2215 8 -0.15925127755866697 2215 9 -0.20503089495417015 2215 10-11 -0.15621121738340804 2215 12-13 -0.2397213839651542 2215 14-15 -0.22812369794035448 2215 16-17 -0.2717132497761483 2215 18-19 -0.23279602131255928 2215 20-21 -0.24158816946959405 2215 22-23 -0.16278366831372182 2215 24-25 -0.17886380525803958 2215 26-27 -0.1495059951235973 2215 28-29 -0.16127507904262472 2215 30-31 -0.22738626271102902 2215 32-33 -0.2734785175673622 2215 34-35 -0.2517248677622135 2215 36-37 -0.1538644697394176 2215 38-39 -0.16041239994319056 2215 40-41 -0.21486484306690556 2215 42-43 -0.17489319285226657 2215 44-45 -0.08588331668800464 2215 46-47 -0.05486934848892133 2215 48-49 -0.029502637805734366 2215 50-51 -0.018299155812840695 2215 52-53 -0.05639841530557277 2215 54-55 -0.05188843229779394 2215 56-57 -0.04778086779141688 2215 58-59 -0.07966720268908745 2215 60-61 -0.20552830140761813 2215 62-63 -0.19705208293480325 2215 64-65 -0.286715409204497 2215 66-67 -0.1972214979196707 2215 68-69 -0.20153003377207312 2215 70-71 -0.16562235722390284 2215 72-73 -0.2572744715751796 2215 74-75 -0.16789286624835853 2215 76-77 -0.0914027904621264 2215 78-79 -0.13874111280392043 2215 80-81 -0.18524256400191774 2215 82-83 -0.13221089055626578 2215 84-85 -0.19531117570780054 2215 86-87 -0.10327852119917935 2215 88-89 -0.16499896821576954 2215 90-91 -0.20153822974003432 2215 92-93 -0.14564469483172005 2215 94-95 -0.25643410233825037 2215 96-97 -0.12920293201438326 2215 98-99 -0.08143727834018577 2215 100 -0.08025709570006256 2216 1 -0.03488209720553925 2216 2 -0.12737958320734677 2216 3 -0.091653824575058 2216 4 -0.08449558315068373 2216 5 -0.03817700743931596 2216 6 -0.10934099015368304 2216 7 -0.1092591588044769 2216 8 -0.04796090533360342 2216 9 -0.11467589586104765 2216 10-11 -0.06407741170959014 2216 12-13 -0.24165699448266764 2216 14-15 -0.20571302037165395 2216 16-17 -0.1995763714320944 2216 18-19 -0.24414270821182527 2216 20-21 -0.1432831315189489 2216 22-23 -0.11669586048692082 2216 24-25 -0.13385149806446606 2216 26-27 -0.14919919045361496 2216 28-29 -0.1867745431747423 2216 30-31 -0.3294685978558576 2216 32-33 -0.2814560334416143 2216 34-35 -0.3402925397574421 2216 36-37 -0.4301972602695727 2216 38-39 -0.397506425369734 2216 40-41 -0.2856883728516664 2216 42-43 -0.338130186216695 2216 44-45 -0.2393844632589932 2216 46-47 -0.1858149686986934 2216 48-49 -0.1949038425408176 2216 50-51 -0.0784940999624979 2216 52-53 -0.10796986948572851 2216 54-55 -0.09350911128922235 2216 56-57 -0.12863000800517455 2216 58-59 -0.12741961574578653 2216 60-61 -0.21529974943187113 2216 62-63 -0.19790624827220427 2216 64-65 -0.19775942619639864 2216 66-67 -0.24529432205368096 2216 68-69 -0.18926722614672542 2216 70-71 -0.0881407367721252 2216 72-73 -0.1839730564956028 2216 74-75 -0.218805466481669 2216 76-77 -0.0828606086839585 2216 78-79 -0.09666950690571241 2216 80-81 0.016984514254602345 2216 82-83 -0.18848342451713052 2216 84-85 -0.06934569872991148 2216 86-87 -0.04119380559357211 2216 88-89 -0.1096162668736369 2216 90-91 -0.09440335999232019 2216 92-93 -0.28145790818560457 2216 94-95 -0.10110606440728631 2216 96-97 -0.1085063315158088 2216 98-99 -0.060273700658235896 2216 100 -0.0647398468046596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28943.0 3 6000.0 4 3327.0 5 2735.0 6 2440.0 7 2575.0 8 3059.0 9 3535.0 10 3820.0 11 4254.0 12 4625.0 13 5190.0 14 5643.0 15 5837.0 16 5798.0 17 5999.0 18 5706.0 19 5623.0 20 5552.0 21 5838.0 22 6981.0 23 10102.0 24 15927.0 25 21314.0 26 21670.0 27 18167.0 28 19253.0 29 24179.0 30 29662.0 31 36295.0 32 47743.0 33 64965.0 34 94296.0 35 148861.0 36 271129.0 37 550976.0 38 856798.0 39 350493.0 40 573.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.41874039006979 20.128583547133864 13.726733707216166 23.725942355580177 2 15.221423667352251 20.357652675093085 40.53880085642021 23.88212280113445 3 18.129787223027925 25.674750234253608 29.851786058970525 26.34367648374794 4 12.234930368195156 16.97799261427623 39.16334580313736 31.62373121439125 5 13.05375480211193 38.08931983674484 35.53192937446227 13.32499598668096 6 30.623817266904485 38.20277834083723 18.170331320621834 13.00307307163645 7 27.214121849109397 32.60872620903278 22.742821928489683 17.434330013368143 8 24.734294334066444 36.868725918718795 20.438572963854263 17.958406783360495 9 24.86323758114373 17.531965487187907 21.087307470919953 36.51748946074841 10-11 23.678420819563513 27.304830465177965 28.925060477463983 20.09168823779454 12-13 24.380052312826262 24.636140985656517 28.769187227383213 22.214619474134008 14-15 21.785669164417804 26.48994286600064 25.841874206793374 25.88251376278819 16-17 21.57638228171835 29.77773093043447 26.397010892931938 22.248875894915248 18-19 21.558809667731833 28.823314631888834 28.136826357716732 21.4810493426626 20-21 22.791021728801226 28.06602146524359 27.592265055013264 21.55069175094192 22-23 22.37767163854581 27.800922934301713 27.657466721172263 22.163938705980215 24-25 22.2382546173945 27.928206494949798 27.67263282465646 22.160906062999242 26-27 21.988573738851695 27.826511280572802 27.760497983873204 22.4244169967023 28-29 22.021028761027175 27.909270454023094 27.820625922109304 22.24907486284043 30-31 22.318332605663326 28.29978007167966 27.370063748330843 22.011823574326176 32-33 21.9294925195944 27.97365654578252 27.875982724936872 22.220868209686202 34-35 22.091492251199675 27.76880164357515 27.934752314142276 22.20495379108291 36-37 22.416185353676997 28.057609287059005 27.354039729051028 22.172165630212966 38-39 22.205848114566397 28.236095605638667 27.574321939037898 21.983734340757035 40-41 22.045551720413723 27.922140127501617 27.809810582131135 22.22249756995353 42-43 22.008125555927457 27.799627563276335 27.808004615700412 22.3842422650958 44-45 22.090243669261014 27.97637330357868 27.750106744337465 22.183276282822845 46-47 22.19280396623104 28.097101573366086 27.616912293796098 22.09318216660678 48-49 22.050073877369115 27.962316069391097 27.700758745545627 22.286851307694157 50-51 22.08534968481121 27.896436119951108 27.69495198664169 22.323262208595988 52-53 22.121925925597175 27.84976229944131 27.677820507415056 22.350491267546456 54-55 22.22948495301886 28.219302501715937 27.454324305933586 22.096888239331612 56-57 22.013867996451918 27.97266119710628 27.61996090600003 22.39350990044177 58-59 21.944564273369213 27.6208804710944 27.962530044804414 22.47202521073198 60-61 22.102720869950616 28.059853111149202 27.55296620550571 22.28445981339447 62-63 22.00596264021108 27.971857284948044 27.582397729884594 22.439782344956285 64-65 22.027607130547718 27.72240063548322 27.74013307278916 22.509859161179904 66-67 21.898104857371987 27.80930565306895 27.62140194068552 22.671187548873547 68-69 22.281339165805154 27.850490550031036 27.574127981423462 22.294042302740348 70-71 22.14849084463371 27.71969865334744 27.6750284088703 22.456782093148554 72-73 22.037444447258892 27.794221756872712 27.738218344528775 22.430115451339617 74-75 22.096332417617973 27.892518510602343 27.566717857484914 22.444431214294774 76-77 22.066022038160035 27.906430261795595 27.49639476408815 22.531152935956218 78-79 22.180014860393097 27.94251220510079 27.60607349701211 22.271399437494 80-81 22.11756693527232 27.7271724789173 27.705302707766062 22.449957878044323 82-83 22.008483723076548 27.73640187940497 27.823313451456162 22.431800946062317 84-85 22.028163869234106 27.779618166908342 27.594517332891638 22.59770063096591 86-87 22.13712249757422 27.887324776818108 27.579483218817803 22.39606950678987 88-89 21.944708383399576 27.70289305850818 27.68976800073944 22.662630557352806 90-91 22.000136051604965 27.67090004051972 27.693378131774853 22.635585776100463 92-93 22.15769007872211 27.75347603814376 27.609489540228505 22.47934434290563 94-95 21.90216405738901 27.540817660136863 27.831947866083436 22.725070416390693 96-97 21.937964849077083 27.558071415089646 27.742619953314744 22.761343782518523 98-99 21.890334902012835 27.6921259584877 27.63577994218618 22.781759197313285 100 22.023092683175737 27.537250050194253 27.609241362429117 22.830415904200898 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 395.0 1 379.0 2 343.5 3 323.0 4 319.5 5 322.0 6 314.5 7 276.5 8 244.0 9 231.0 10 223.0 11 232.0 12 242.0 13 252.0 14 285.0 15 336.0 16 408.0 17 512.0 18 765.0 19 1128.5 20 1573.0 21 2269.5 22 3445.0 23 5162.5 24 7639.5 25 10871.5 26 14971.5 27 20426.0 28 27301.0 29 34982.0 30 42484.0 31 50244.0 32 58046.0 33 66050.0 34 73678.0 35 79383.5 36 84325.0 37 89418.5 38 93509.5 39 95864.5 40 96839.5 41 97292.0 42 97788.5 43 97526.5 44 95125.0 45 92550.0 46 90906.5 47 88530.0 48 86403.0 49 84870.5 50 82956.0 51 80059.5 52 75716.0 53 72264.0 54 69768.5 55 66157.5 56 61691.0 57 56850.5 58 51668.0 59 46819.5 60 41902.0 61 36230.5 62 31307.0 63 26455.0 64 21717.0 65 17986.5 66 14656.5 67 11668.5 68 9485.5 69 7879.0 70 6163.0 71 4668.0 72 3748.5 73 3061.0 74 2440.5 75 1754.0 76 1169.0 77 839.0 78 616.0 79 448.5 80 321.5 81 211.0 82 128.5 83 71.0 84 45.0 85 33.5 86 25.5 87 18.0 88 12.0 89 11.0 90 7.5 91 5.5 92 5.5 93 5.0 94 3.0 95 2.5 96 2.5 97 1.5 98 2.0 99 1.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.060941289774908966 2 0.04198259865633511 3 0.05724563848473862 4 0.07457824303563754 5 0.0881412832705627 6 0.08865867445118654 7 0.09021084799305808 8 0.10340432309896622 9 0.09811954175402261 10-11 0.10384780125378666 12-13 0.10765432208266212 14-15 0.10538149653920735 16-17 0.07633367739846846 18-19 0.0999673673991078 20-21 0.09200323886879071 22-23 0.058151073050830365 24-25 0.022044559945866102 26-27 0.1815303913731673 28-29 0.06742715778915792 30-31 0.0756684601662378 32-33 0.07337715636633217 34-35 0.020732603737855626 36-37 0.05131411816401522 38-39 0.07718367719520763 40-41 0.08244998028370037 42-43 0.07642606868072271 44-45 0.0736358519566441 46-47 0.07973367658542516 48-49 0.07679563380973974 50-51 0.0719912871325183 52-53 0.07616737309041077 54-55 0.06722389696819855 56-57 0.06975541810196524 58-59 0.06639237542791022 60-61 0.0639162890634961 62-63 0.0694597659987516 64-65 0.06648476671016448 66-67 0.06794454896978176 68-69 0.06750107081496133 70-71 0.059943463926562975 72-73 0.058520638179847394 74-75 0.059943463926562975 76-77 0.0548065086332262 78-79 0.05094455303499819 80-81 0.0456967282029563 82-83 0.05210868319140185 84-85 0.04216738122084362 86-87 0.04000542521609397 88-89 0.04187172911763 90-91 0.03795433875004943 92-93 0.04122499014185019 94-95 0.03989455567738886 96-97 0.04340542440305069 98-99 0.04310977229983706 100 0.05136955293336778 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2705883.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.0795724738205 #Duplication Level Percentage of deduplicated Percentage of total 1 77.25987567621175 50.28039678388385 2 13.559428360708656 17.64883601408632 3 4.380884154085626 8.55318203415682 4 1.8167151072753382 4.729241699528399 5 0.9143194926886354 2.9751760844328423 6 0.5503727459889987 2.1490813806123987 7 0.3467524567882968 1.5796551149420492 8 0.2283225117133946 1.1887305158765278 9 0.15953271789227474 0.9344088968414269 >10 0.750507453669666 8.236383396081031 >50 0.027734740222336476 1.218759061654206 >100 0.00549777563614761 0.482303477477819 >500 5.680711890521079E-5 0.023845540426242897 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.108695387051103E-4 0.0 0.0 0.0 3 0.0 1.108695387051103E-4 0.0 3.695651290170344E-5 0.0 4 0.0 1.108695387051103E-4 0.0 3.695651290170344E-5 0.0 5 0.0 1.108695387051103E-4 0.0 3.695651290170344E-5 0.0 6 0.0 1.108695387051103E-4 0.0 3.695651290170344E-5 0.0 7 0.0 1.108695387051103E-4 0.0 3.695651290170344E-5 0.0 8 0.0 1.108695387051103E-4 0.0 3.695651290170344E-5 0.0 9 0.0 1.108695387051103E-4 0.0 3.695651290170344E-5 0.0 10-11 0.0 1.108695387051103E-4 0.0 3.695651290170344E-5 0.0 12-13 0.0 1.108695387051103E-4 0.0 5.543476935255516E-5 0.0 14-15 0.0 1.4782605160681376E-4 0.0 1.4782605160681376E-4 0.0 16-17 0.0 2.032608209593689E-4 0.0 2.217390774102206E-4 0.0 18-19 0.0 2.217390774102206E-4 0.0 2.217390774102206E-4 0.0 20-21 0.0 2.217390774102206E-4 0.0 2.217390774102206E-4 0.0 22-23 0.0 2.217390774102206E-4 0.0 2.771738467627758E-4 1.847825645085172E-5 24-25 0.0 2.217390774102206E-4 0.0 3.326086161153309E-4 3.695651290170344E-5 26-27 0.0 2.217390774102206E-4 0.0 4.2499989836958956E-4 3.695651290170344E-5 28-29 0.0 2.217390774102206E-4 0.0 9.978258483459928E-4 3.695651290170344E-5 30-31 0.0 2.4021733386107234E-4 0.0 0.003603260007916085 3.695651290170344E-5 32-33 0.0 2.5869559031192405E-4 0.0 0.009941301970558224 3.695651290170344E-5 34-35 0.0 2.956521032136275E-4 0.0 0.017905430500875313 3.695651290170344E-5 36-37 0.0 3.326086161153309E-4 0.0 0.03344564417604161 3.695651290170344E-5 38-39 0.0 4.61956411271293E-4 0.0 0.06256737634258391 3.695651290170344E-5 40-41 0.0 4.804346677221447E-4 0.0 0.09721410718793089 3.695651290170344E-5 42-43 0.0 5.543476935255515E-4 0.0 0.13289562039452557 3.695651290170344E-5 44-45 0.0 5.91304206427255E-4 0.0 0.17504452335891835 3.695651290170344E-5 46-47 0.0 6.652172322306618E-4 0.0 0.22879777137444599 3.695651290170344E-5 48-49 0.0 7.021737451323653E-4 0.0 0.28480536667697753 3.695651290170344E-5 50-51 0.0 7.206520015832171E-4 0.0 0.34759448209697164 3.695651290170344E-5 52-53 0.0 7.760867709357722E-4 0.0 0.4145412052184074 3.695651290170344E-5 54-55 0.0 7.760867709357722E-4 0.0 0.48529444916871867 3.695651290170344E-5 56-57 0.0 7.760867709357722E-4 0.0 0.5689085596088227 3.695651290170344E-5 58-59 0.0 8.49999796739179E-4 0.0 0.666067232027401 3.695651290170344E-5 60-61 0.0 8.49999796739179E-4 0.0 0.7688063378941365 3.695651290170344E-5 62-63 0.0 8.49999796739179E-4 0.0 0.8793432679831316 3.695651290170344E-5 64-65 0.0 8.49999796739179E-4 0.0 0.9991932393233558 3.695651290170344E-5 66-67 0.0 8.869563096408825E-4 0.0 1.1373921193192758 3.695651290170344E-5 68-69 0.0 9.23912822542586E-4 0.0 1.291630125914535 3.695651290170344E-5 70-71 0.0 9.23912822542586E-4 0.0 1.4598007378737365 3.695651290170344E-5 72-73 0.0 9.23912822542586E-4 0.0 1.6368593911857978 3.695651290170344E-5 74-75 0.0 9.23912822542586E-4 0.0 1.8189810867653922 3.695651290170344E-5 76-77 0.0 9.608693354442894E-4 0.0 2.018195169562025 3.695651290170344E-5 78-79 0.0 9.608693354442894E-4 0.0 2.248027723297718 3.695651290170344E-5 80-81 0.0 9.608693354442894E-4 0.0 2.4944167948133753 3.695651290170344E-5 82-83 0.0 9.608693354442894E-4 0.0 2.760669992013698 3.695651290170344E-5 84-85 0.0 9.793475918951412E-4 0.0 3.0277177542414067 3.695651290170344E-5 86-87 0.0 9.978258483459928E-4 0.0 3.3084209479862947 3.695651290170344E-5 88 0.0 9.978258483459928E-4 0.0 3.541062196702518 3.695651290170344E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3220 0.0 35.621624 1 GGTATCA 2080 0.0 34.578636 1 TCAACGC 3175 0.0 34.346603 4 ATCAACG 3305 0.0 32.995605 3 CAACGCA 3375 0.0 32.31125 5 AACGCAG 3610 0.0 30.202856 6 TATCAAC 3885 0.0 28.67457 2 GTACATG 7535 0.0 26.015522 1 TACATGG 7560 0.0 25.18099 2 ACGCAGA 4385 0.0 24.650488 7 CGCAGAG 4610 0.0 23.361416 8 ACATGGG 8195 0.0 22.541517 3 ACCGATC 200 5.934071E-7 21.162994 8 TATACCG 230 1.0089025E-7 20.436745 5 GAGTACA 5635 0.0 19.103867 1 GCAGAGT 5570 0.0 19.000114 9 CATGGGG 5910 0.0 18.531391 4 AGTACAT 5810 0.0 16.908682 2 TATACTG 1705 0.0 16.541178 5 TAATACG 310 2.3032117E-6 15.162745 4 >>END_MODULE