FastQCFastQC Report
Wed 25 May 2016
SRR1033006_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033006_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2705883
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51990.19213691057595617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41710.15414561531300502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39200.053.747841
GTATCAA74450.045.203611
ATCAACG89650.036.6986123
TCAACGC89950.036.5748634
CAACGCA92400.035.7061425
TATCAAC95500.034.778282
AACGCAG95150.034.674176
ACGCAGA109100.030.111357
CGCAGAG112350.029.1566418
GCAGAGT123500.026.0295689
GTACATG75750.023.7072931
GTGGTAT16750.023.0748371
TACATGG76850.022.8045442
AGAGTAC114800.021.90209610-11
ACATGGG78900.021.6833443
GAGTACT87450.021.227912-13
CAGAGTA122150.021.10361910-11
AGTACTT92600.019.53977412-13
GTACTTT95150.019.23837714-15
TGGTATC19250.018.5996692
Produced by FastQC (version 0.11.5)