FastQCFastQC Report
Wed 25 May 2016
SRR1033005_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033005_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4513405
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44350.036.349561
GTATCAA69300.035.19921
TCAACGC72500.032.0904274
ATCAACG75750.030.6508833
CAACGCA77050.030.073075
AACGCAG79600.029.1090286
GTACATG163000.028.3730581
TATCAAC85500.027.8702262
TACATGG168000.026.3815982
ACATGGG172750.024.1598153
ACGCAGA97450.023.3912687
CGCAGAG102250.021.9714368
TACACCG9300.021.2260825
CATGGGG121400.020.4420074
GAGTACA116150.018.6543621
GCAGAGT118900.018.1436529
TATACCG2352.7995175E-618.0002355
GTATAGG19050.017.0236531
AGTACAT122150.016.8146062
ACCGTCC4551.0913936E-1116.5273198
Produced by FastQC (version 0.11.5)