FastQCFastQC Report
Wed 25 May 2016
SRR1033005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033005_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4513405
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113840.2522264232879611No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85480.1893913796789785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47590.1054414571703625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74350.051.941341
GTATCAA135800.043.2100751
TCAACGC164950.035.31354
ATCAACG165300.035.1537253
CAACGCA168850.034.5505145
TATCAAC171750.033.8883362
AACGCAG172200.033.7960246
ACGCAGA198300.029.1804647
CGCAGAG205350.028.0179758
GTACATG158750.026.4277421
GCAGAGT223900.025.4901479
TACATGG160950.025.1995932
ACATGGG163250.023.8357933
GAGTACT144300.021.3546512-13
CAGAGTA220150.021.12353910-11
AGAGTAC207600.020.97502910-11
CATGGGG111500.020.42414
GTACTTT154850.019.68740514-15
GTGGTAT34150.019.6728691
AGTACTT148700.019.55395712-13
Produced by FastQC (version 0.11.5)