##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033005_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4513405
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86507570226913	34.0	31.0	34.0	31.0	34.0
2	32.91846111749334	34.0	31.0	34.0	31.0	34.0
3	32.992146948922155	34.0	33.0	34.0	31.0	34.0
4	36.38725551994558	37.0	37.0	37.0	35.0	37.0
5	36.372494380628375	37.0	37.0	37.0	35.0	37.0
6	36.42758604645495	37.0	37.0	37.0	35.0	37.0
7	36.393465465651765	37.0	37.0	37.0	35.0	37.0
8	36.33808687675934	37.0	37.0	37.0	35.0	37.0
9	38.18806267108757	39.0	39.0	39.0	37.0	39.0
10-11	38.195174818125125	39.0	39.0	39.0	37.0	39.0
12-13	38.188105211032465	39.0	39.0	39.0	37.0	39.0
14-15	39.741247240165684	41.0	40.0	41.0	37.0	41.0
16-17	39.75681730312259	41.0	40.0	41.0	37.0	41.0
18-19	39.783644831341306	41.0	40.0	41.0	37.5	41.0
20-21	39.79203683250229	41.0	40.0	41.0	37.0	41.0
22-23	39.755572566609914	41.0	40.0	41.0	37.0	41.0
24-25	39.73400581157685	41.0	40.0	41.0	37.0	41.0
26-27	39.659306000680196	41.0	40.0	41.0	37.0	41.0
28-29	39.59719070192017	41.0	40.0	41.0	37.0	41.0
30-31	39.47944811068362	41.0	39.5	41.0	36.5	41.0
32-33	39.3526545922646	41.0	39.0	41.0	35.5	41.0
34-35	39.25843969242733	41.0	39.0	41.0	35.0	41.0
36-37	39.167521859881845	41.0	39.0	41.0	35.0	41.0
38-39	39.0709516429392	41.0	39.0	41.0	35.0	41.0
40-41	38.96607439394426	41.0	39.0	41.0	35.0	41.0
42-43	38.823607010671545	41.0	39.0	41.0	35.0	41.0
44-45	38.63975424319333	41.0	39.0	41.0	35.0	41.0
46-47	38.400803052241045	41.0	38.5	41.0	35.0	41.0
48-49	38.182714380827775	41.0	38.0	41.0	34.0	41.0
50-51	38.017127756095455	40.5	38.0	41.0	34.0	41.0
52-53	37.87102088113076	40.0	38.0	41.0	34.0	41.0
54-55	37.71599092038051	40.0	37.0	41.0	34.0	41.0
56-57	37.49716322820576	40.0	37.0	41.0	33.0	41.0
58-59	37.32230444642127	40.0	36.0	41.0	33.0	41.0
60-61	37.12551122711124	39.5	36.0	41.0	33.0	41.0
62-63	36.8834573897091	39.0	35.0	41.0	33.0	41.0
64-65	36.59440477865381	39.0	35.0	41.0	32.0	41.0
66-67	36.31438514824174	38.5	35.0	41.0	32.0	41.0
68-69	36.02169071909124	37.5	35.0	40.0	32.0	41.0
70-71	35.69879104578472	37.0	35.0	40.0	31.0	41.0
72-73	35.36110220554105	36.5	35.0	39.0	31.0	41.0
74-75	35.01927879284044	36.0	35.0	39.0	31.0	41.0
76-77	34.67779636438564	35.5	35.0	38.5	31.0	40.0
78-79	34.34247679966677	35.0	35.0	37.0	31.0	39.0
80-81	34.02575040352018	35.0	34.5	37.0	30.0	39.0
82-83	33.72766614119495	35.0	34.0	36.5	30.0	39.0
84-85	33.46050897271572	35.0	34.0	36.0	30.0	37.0
86-87	33.31933794995131	35.0	34.0	36.0	30.0	37.0
88-89	33.13882346033648	35.0	34.0	35.5	30.0	37.0
90-91	32.97612678232953	35.0	34.0	35.0	30.0	36.0
92-93	32.82358983960003	35.0	34.0	35.0	30.0	36.0
94-95	32.729664521575174	35.0	34.0	35.0	29.5	36.0
96-97	32.66916817790559	35.0	34.0	35.0	30.0	36.0
98-99	32.56666995317283	35.0	34.0	35.0	30.0	36.0
100	32.488421491091536	35.0	34.0	35.0	30.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19379433251029354
1101	2	0.21294611677500086
1101	3	0.2107940307683549
1101	4	0.10443172391680378
1101	5	0.10536242640937843
1101	6	0.12701849031001444
1101	7	0.1088145963766678
1101	8	0.02364911836098571
1101	9	0.1026928955739308
1101	10-11	0.20684368656324637
1101	12-13	0.23106139628878708
1101	14-15	0.278442398019358
1101	16-17	0.2664235887844697
1101	18-19	0.28448766205004716
1101	20-21	0.28519353606946396
1101	22-23	0.24303711142017193
1101	24-25	0.24871157533938515
1101	26-27	0.30052473534505
1101	28-29	0.2681753966967264
1101	30-31	0.2614549163503028
1101	32-33	0.30724693999930963
1101	34-35	0.26762448940297645
1101	36-37	0.2994388434428785
1101	38-39	0.3102526666307597
1101	40-41	0.3616509052610297
1101	42-43	0.38261160434377217
1101	44-45	0.390526478811303
1101	46-47	0.46900934262880156
1101	48-49	0.42270676665745555
1101	50-51	0.40586306817462514
1101	52-53	0.43797512400479377
1101	54-55	0.41661218876190276
1101	56-57	0.39882755523181856
1101	58-59	0.4065988204237101
1101	60-61	0.42836930558630826
1101	62-63	0.3746120708243481
1101	64-65	0.4228239013346027
1101	66-67	0.4043522663894876
1101	68-69	0.33140162005756935
1101	70-71	0.37687950282482063
1101	72-73	0.35136622659530303
1101	74-75	0.35756764082747594
1101	76-77	0.35940337666178124
1101	78-79	0.38490079631162644
1101	80-81	0.32988800335020585
1101	82-83	0.3181120994005795
1101	84-85	0.387984961871787
1101	86-87	0.44440340583812343
1101	88-89	0.41410704672556875
1101	90-91	0.39075388588862836
1101	92-93	0.4448531195798182
1101	94-95	0.4567528478342382
1101	96-97	0.4528392626317057
1101	98-99	0.4703661749000716
1101	100	0.4808363443739978
1102	1	0.16406964422831294
1102	2	0.20693242510369458
1102	3	0.18634415696072892
1102	4	0.10662550917742664
1102	5	0.13659335980292298
1102	6	0.12454463591908649
1102	7	0.11223375127747204
1102	8	0.0372018302203827
1102	9	0.09923288605910585
1102	10-11	0.1467165803331838
1102	12-13	0.18773991616912866
1102	14-15	0.20942484397434669
1102	16-17	0.2070646568100898
1102	18-19	0.20741153538998702
1102	20-21	0.1994183332074968
1102	22-23	0.2193976103840214
1102	24-25	0.1649662017212492
1102	26-27	0.19887119859874502
1102	28-29	0.19613070389923593
1102	30-31	0.24156732029493355
1102	32-33	0.2091289400476839
1102	34-35	0.19929111575258673
1102	36-37	0.22418303173964915
1102	38-39	0.22649976716267162
1102	40-41	0.25548454634787277
1102	42-43	0.2419318319469781
1102	44-45	0.2280111247335057
1102	46-47	0.24105484176735814
1102	48-49	0.1746845869025293
1102	50-51	0.1851698030039941
1102	52-53	0.1833154133737267
1102	54-55	0.16531169501239162
1102	56-57	0.15123461770443924
1102	58-59	0.14396614372078886
1102	60-61	0.17740317459890065
1102	62-63	0.1675043087164667
1102	64-65	0.16027535288291972
1102	66-67	0.13228311457965702
1102	68-69	0.11290670941360048
1102	70-71	0.136544978794511
1102	72-73	0.12816389680718032
1102	74-75	0.13730213959242832
1102	76-77	0.11686256908432568
1102	78-79	0.07487985832193544
1102	80-81	0.12666411737758665
1102	82-83	0.13484582676591117
1102	84-85	0.1214821168317215
1102	86-87	0.15499375501067902
1102	88-89	0.12006080037762246
1102	90-91	0.10558263248519495
1102	92-93	0.12206188934526097
1102	94-95	0.12136764526792376
1102	96-97	0.15531661116725815
1102	98-99	0.13417825980240394
1102	100	0.1287719768544875
1103	1	0.15754870214890815
1103	2	0.1896179136658418
1103	3	0.18623934615783355
1103	4	0.08089115338815134
1103	5	0.11594977742409185
1103	6	0.10569346078140285
1103	7	0.09657483594620686
1103	8	0.03410881030892199
1103	9	0.07006944209790333
1103	10-11	0.0917369659695666
1103	12-13	0.11347427195840964
1103	14-15	0.15443175578687374
1103	16-17	0.21656914398210603
1103	18-19	0.2505166299205257
1103	20-21	0.20204870864782265
1103	22-23	0.18075261066256587
1103	24-25	0.12371783898517918
1103	26-27	0.11211486622542566
1103	28-29	0.12860585829758975
1103	30-31	0.1645736154209061
1103	32-33	0.2035215620926749
1103	34-35	0.1995774345095782
1103	36-37	0.18787794290958004
1103	38-39	0.15806874212856314
1103	40-41	0.12067174828810323
1103	42-43	0.09106633309808387
1103	44-45	0.09054139225369084
1103	46-47	0.11464387977520829
1103	48-49	0.07957351206709973
1103	50-51	0.040344140033560905
1103	52-53	0.023630778585605583
1103	54-55	0.05770170520844431
1103	56-57	0.03709306222920361
1103	58-59	0.05351293092635956
1103	60-61	0.03569061271971208
1103	62-63	0.07283080926642782
1103	64-65	0.12745959576413668
1103	66-67	0.1329693727044301
1103	68-69	0.12926840125947336
1103	70-71	0.12850745419507348
1103	72-73	0.13525553306057247
1103	74-75	0.1500781100640225
1103	76-77	0.10773068496440175
1103	78-79	0.16183745593914978
1103	80-81	0.1970993089326072
1103	82-83	0.14583917147426462
1103	84-85	0.1028244335780002
1103	86-87	0.08679755022410518
1103	88-89	0.11366202960493865
1103	90-91	0.12151614175557768
1103	92-93	0.10407439927248419
1103	94-95	0.11743743449327582
1103	96-97	0.12062980631616682
1103	98-99	0.07893603542955674
1103	100	0.06907620025048544
1104	1	0.12708545546804118
1104	2	0.16267979857501302
1104	3	0.13794208057967694
1104	4	0.10475330843884478
1104	5	0.08038851904574784
1104	6	0.10768321129404512
1104	7	0.08941007077738305
1104	8	0.03705021937395969
1104	9	0.074860852815128
1104	10-11	0.10501485271729649
1104	12-13	0.13943407077054104
1104	14-15	0.1710848522600017
1104	16-17	0.14161055070809425
1104	18-19	0.1346937842490803
1104	20-21	0.128655087269955
1104	22-23	0.15095617841681985
1104	24-25	0.1448038034695216
1104	26-27	0.16367272815696055
1104	28-29	0.18325705605745668
1104	30-31	0.22894537857702346
1104	32-33	0.24960701607041358
1104	34-35	0.20812981230079686
1104	36-37	0.1839600368257166
1104	38-39	0.17960046240956729
1104	40-41	0.14160255589468562
1104	42-43	0.2178791048964328
1104	44-45	0.18613640718461255
1104	46-47	0.24646095282368918
1104	48-49	0.2373061647744663
1104	50-51	0.2053730272814036
1104	52-53	0.24070616611580675
1104	54-55	0.23308663646454875
1104	56-57	0.29453103196488684
1104	58-59	0.268107887566714
1104	60-61	0.2866304460829312
1104	62-63	0.20910187601730712
1104	64-65	0.24632695426934248
1104	66-67	0.27529204034829746
1104	68-69	0.2574591324645752
1104	70-71	0.21635082557320828
1104	72-73	0.2234910106329977
1104	74-75	0.24159174541677686
1104	76-77	0.26598661621567743
1104	78-79	0.2655947102378917
1104	80-81	0.21338732821527628
1104	82-83	0.2836916948434052
1104	84-85	0.31835578926447283
1104	86-87	0.323420959346322
1104	88-89	0.3516628040898553
1104	90-91	0.3535402020243197
1104	92-93	0.3269790667136334
1104	94-95	0.32411051683557446
1104	96-97	0.27087872933287827
1104	98-99	0.27609862119930284
1104	100	0.27607804884128484
1105	1	0.074990117630783
1105	2	0.11160333542326839
1105	3	0.11566368677099348
1105	4	0.0876552007003184
1105	5	0.06745326719880751
1105	6	0.08914545110104655
1105	7	0.09276869150721723
1105	8	-0.02107941598370644
1105	9	0.09448492959166543
1105	10-11	0.07083661069550828
1105	12-13	0.039961983593933326
1105	14-15	0.09665913020425876
1105	16-17	0.1237234816927213
1105	18-19	0.1668847162407019
1105	20-21	0.15582198858611918
1105	22-23	0.13668009259870928
1105	24-25	0.14848853940251416
1105	26-27	0.15715544434719675
1105	28-29	0.1532035799367435
1105	30-31	0.16590703629509562
1105	32-33	0.14211656556374663
1105	34-35	0.16300381790576068
1105	36-37	0.13846693638289054
1105	38-39	0.12669091994459336
1105	40-41	0.14132840742087183
1105	42-43	0.09088952746397183
1105	44-45	0.09173302383675974
1105	46-47	0.12667500128362263
1105	48-49	0.1553065139189016
1105	50-51	0.17720189763292638
1105	52-53	0.1676027836062488
1105	54-55	0.18137618868681926
1105	56-57	0.24661259252115286
1105	58-59	0.20597679036801253
1105	60-61	0.2547183394523671
1105	62-63	0.2074855082664797
1105	64-65	0.18744210226428493
1105	66-67	0.2523129067484149
1105	68-69	0.2834274600632156
1105	70-71	0.2527514178494741
1105	72-73	0.3050606128266793
1105	74-75	0.26517222015374387
1105	76-77	0.2789788195249656
1105	78-79	0.30297619377115126
1105	80-81	0.29249472096027773
1105	82-83	0.27165132688912763
1105	84-85	0.27282539212594514
1105	86-87	0.22798288277788004
1105	88-89	0.2551147896972594
1105	90-91	0.2565064425709025
1105	92-93	0.2506588264386167
1105	94-95	0.23349544767282282
1105	96-97	0.25418841064102793
1105	98-99	0.2517060849145949
1105	100	0.2272458285123946
1106	1	0.06042240140829591
1106	2	0.10450354412540719
1106	3	0.06550363548338112
1106	4	0.05636211697648719
1106	5	0.05668483076482289
1106	6	0.061071987112775616
1106	7	0.028494830602106447
1106	8	0.007400368542221258
1106	9	0.03012963203412511
1106	10-11	0.07253383952905779
1106	12-13	0.07304858074649445
1106	14-15	0.09750007091361113
1106	16-17	0.08562200322358393
1106	18-19	0.11223718074719358
1106	20-21	0.10308701115113905
1106	22-23	0.1186610697050412
1106	24-25	0.11971421392026826
1106	26-27	0.1200480061317819
1106	28-29	0.11458261644874312
1106	30-31	0.10331811039935701
1106	32-33	0.14972731680017404
1106	34-35	0.08387572543005462
1106	36-37	0.08071267319570552
1106	38-39	0.08922109203133743
1106	40-41	0.16138602561197501
1106	42-43	0.11349394244299305
1106	44-45	0.10969158790538813
1106	46-47	0.1226949126019079
1106	48-49	0.11517387547534241
1106	50-51	0.06298847966056798
1106	52-53	0.05961463807661005
1106	54-55	0.048845165681271396
1106	56-57	0.09518727855867581
1106	58-59	0.08287679919051527
1106	60-61	0.03534894157095181
1106	62-63	0.04839977965045961
1106	64-65	0.053105340115664035
1106	66-67	0.05197484815189313
1106	68-69	0.0821662521935167
1106	70-71	0.1063713735390337
1106	72-73	0.08354304228430465
1106	74-75	0.06279361377801962
1106	76-77	0.09686326193681083
1106	78-79	0.0894744349710308
1106	80-81	0.09795591917654178
1106	82-83	0.04340493658520472
1106	84-85	0.024076115622598593
1106	86-87	0.0286106783174489
1106	88-89	-0.003918775870914715
1106	90-91	0.03278542824118347
1106	92-93	0.06136780753237048
1106	94-95	0.042918821685731245
1106	96-97	-0.03201446093294891
1106	98-99	-0.06781642159250367
1106	100	-0.06050959526628219
1107	1	0.07803928778259461
1107	2	0.08322174592850473
1107	3	0.01711134928525837
1107	4	0.0669543903897889
1107	5	0.054986810571293177
1107	6	0.052090211993572666
1107	7	0.02061738293051718
1107	8	0.029351506662784743
1107	9	0.03991336258195588
1107	10-11	0.030485937595187806
1107	12-13	0.045075268533409485
1107	14-15	0.05696438661652081
1107	16-17	0.06503612222432054
1107	18-19	0.08405079528895953
1107	20-21	0.05929044810297768
1107	22-23	0.0703763277472973
1107	24-25	0.05904385554419633
1107	26-27	0.08153004940734832
1107	28-29	0.0825623427450779
1107	30-31	0.1020395842516777
1107	32-33	0.08458978780407733
1107	34-35	0.1103608078109346
1107	36-37	0.107510528811666
1107	38-39	0.06419487771644583
1107	40-41	0.11854550767475303
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2216	20-21	-0.29887068369434644
2216	22-23	-0.3148298224083774
2216	24-25	-0.31410450879540264
2216	26-27	-0.3367074278985598
2216	28-29	-0.33972223286981773
2216	30-31	-0.3528709916967614
2216	32-33	-0.3870003331648846
2216	34-35	-0.4126449930651148
2216	36-37	-0.3783923745643918
2216	38-39	-0.38889603954437746
2216	40-41	-0.3373844514780231
2216	42-43	-0.37431094717785385
2216	44-45	-0.34260666962119046
2216	46-47	-0.35880475565763703
2216	48-49	-0.38552310721614447
2216	50-51	-0.34998929465115225
2216	52-53	-0.3283580222288407
2216	54-55	-0.32321650801308976
2216	56-57	-0.40788001814394903
2216	58-59	-0.418310883846857
2216	60-61	-0.40143274175167676
2216	62-63	-0.3457821303806625
2216	64-65	-0.38120383693235027
2216	66-67	-0.36597644992004774
2216	68-69	-0.41452091202766184
2216	70-71	-0.3599292712677027
2216	72-73	-0.33370479766856675
2216	74-75	-0.37712277395469584
2216	76-77	-0.34533785345690404
2216	78-79	-0.3421351301686002
2216	80-81	-0.3820494189656216
2216	82-83	-0.37445480052227964
2216	84-85	-0.37008477782404015
2216	86-87	-0.3298078844999708
2216	88-89	-0.27278713946042643
2216	90-91	-0.31498275194348224
2216	92-93	-0.29443649249947157
2216	94-95	-0.2972161297395104
2216	96-97	-0.28917952155006077
2216	98-99	-0.2968342658149439
2216	100	-0.3364125400464886
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	17.0
3	12.0
4	15.0
5	42.0
6	92.0
7	262.0
8	562.0
9	975.0
10	1695.0
11	2742.0
12	4570.0
13	7200.0
14	8322.0
15	11117.0
16	14524.0
17	21493.0
18	26504.0
19	14528.0
20	9641.0
21	8912.0
22	9514.0
23	9567.0
24	10529.0
25	12453.0
26	15466.0
27	19797.0
28	25373.0
29	32451.0
30	41606.0
31	52400.0
32	67413.0
33	88436.0
34	125325.0
35	198068.0
36	387956.0
37	898021.0
38	1579924.0
39	802944.0
40	2937.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.91544243823984	19.77811785251298	13.920521329103842	23.385918380143337
2	15.509012975192437	20.29225534200296	40.12979623705682	24.068935445747776
3	18.063636740292626	26.09332788880398	30.187625778266003	25.65540959263739
4	12.367660999432287	17.026920065005598	38.72471050393521	31.880708431626896
5	12.782028353055452	38.0475502151826	35.32863204838241	13.841789383379538
6	30.38182819680965	38.25711230572135	17.732007691158934	13.629051806310066
7	27.199480299988497	32.938624837787614	22.448731802135566	17.41316306008832
8	25.73736552585003	35.7613876616258	20.000874177657305	18.500372634866867
9	25.779597957349658	16.435810900634383	20.937719903078687	36.84687123893727
10-11	24.24419036182217	26.674694604184644	28.416206832757084	20.664908201236095
12-13	25.214810294941664	24.036087198988078	28.83814223464744	21.910960271422812
14-15	22.247428655748948	25.577547116385478	26.519553560310875	25.65547066755469
16-17	21.45210766594179	29.19847653822336	26.92440629635497	22.425009499479884
18-19	21.430609804544115	27.667054327372487	29.643492473629063	21.25884339445433
20-21	22.4067416950174	26.84811134830577	29.528870110260435	21.2162768464164
22-23	22.174122227408017	26.563176285010066	29.520969619677945	21.741731867903972
24-25	21.794706190586442	26.75627051536707	29.567608347171802	21.881414946874685
26-27	21.6198241593204	26.866671711218814	29.531458229723366	21.98204589973742
28-29	21.741056259203212	26.865041129236772	29.632139645411215	21.761762966148797
30-31	21.820106068667418	27.071797004788888	29.330082829998734	21.778014096544958
32-33	21.74261969672711	26.88754438264837	29.552580800103712	21.81725512052081
34-35	21.884346174721152	26.7266107250842	29.54692681430617	21.84211628588848
36-37	22.07902009181486	26.94144630568426	29.07961499110754	21.89991861139334
38-39	21.897128741961158	27.194034651130806	29.116795924684602	21.792040682223437
40-41	21.86120089905933	26.95289249218067	29.20080683902258	21.985099769737417
42-43	21.92733020529992	26.980263767626226	29.034704142759647	22.057701884314206
44-45	21.927101967502047	27.07701412050864	28.9021266269882	22.093757285001107
46-47	22.105782224497634	27.214051975869168	28.585641109365117	22.094524690268077
48-49	22.12833871705647	27.287795815945987	28.320805201647005	22.263060265350543
50-51	22.170453288898486	27.401895690128164	28.141308226047645	22.286342794925705
52-53	22.229785825806882	27.36955390043503	28.15412805291772	22.24653222084037
54-55	22.189923394140138	27.618281903220122	28.020803999400627	22.17099070323911
56-57	22.035950561186226	27.465029337065815	28.09769108182253	22.401329019925427
58-59	22.03957446511246	27.038381902048826	28.395453246593544	22.526590386245175
60-61	22.18544735356227	27.36982043938526	28.15125929283731	22.293472914215155
62-63	21.973971153796043	27.29633562750024	28.258969821516704	22.47072339718701
64-65	22.088810997462005	27.172212553493424	28.24436318034832	22.494613268696252
66-67	21.927119752630166	27.231719119316594	28.21186625438361	22.62929487366963
68-69	22.116991155893306	27.332226850660952	28.120756387478092	22.43002560596765
70-71	22.166656881002258	27.17628929821277	28.125727693393348	22.531326127391626
72-73	22.07710143450455	27.26334109170349	28.093545781953978	22.566011691837982
74-75	22.145841111090185	27.390196536761046	27.92113714590204	22.542825206246725
76-77	22.19353605107633	27.260766933678855	27.84614576764933	22.699551247595494
78-79	22.137936245015958	27.388762450691317	27.905334864771692	22.567966439521037
80-81	22.127587825833025	27.335078066545503	27.9450404606911	22.59229364693037
82-83	22.039849138038473	27.284545622228045	28.02764436325407	22.647960876479413
84-85	22.03708729883536	27.298115281035052	27.819816745893622	22.84498067423597
86-87	22.168255462929626	27.29522807211898	27.83839144764173	22.69812501730966
88-89	21.939074337877855	27.353712639879753	27.85547567341633	22.851737348826067
90-91	21.956307883192274	27.272643683254312	27.879226303895067	22.89182212965835
92-93	22.077014241898464	27.317728891452138	27.751362451098103	22.853894415551295
94-95	21.865632347266857	27.14885693518846	27.842750777938573	23.142759939606112
96-97	21.99399809389796	27.115747232213767	27.84700312923773	23.043251544650545
98-99	21.86092663582814	27.231365608470064	27.792793551294565	23.11491420440723
100	21.896506074681977	27.013529696537315	27.795267652692367	23.29469657608834
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	85.0
1	87.0
2	287.0
3	490.5
4	332.5
5	237.5
6	644.5
7	1602.5
8	2955.5
9	3476.0
10	2697.0
11	2067.0
12	2187.5
13	2512.0
14	2814.5
15	3174.5
16	3581.5
17	4037.0
18	4561.5
19	5083.0
20	5788.5
21	7032.0
22	8925.0
23	11731.0
24	15744.0
25	21238.0
26	28674.5
27	37433.0
28	48000.0
29	60031.0
30	71015.5
31	81259.0
32	90502.0
33	100010.0
34	109724.5
35	117901.5
36	125567.0
37	133758.0
38	140488.0
39	144060.5
40	149129.0
41	156158.5
42	158533.0
43	159904.0
44	159633.0
45	156739.5
46	156303.5
47	153323.5
48	150064.5
49	147217.0
50	142364.5
51	137778.0
52	130217.5
53	123878.5
54	120433.5
55	113788.5
56	104165.5
57	94643.0
58	86501.0
59	80668.5
60	74147.0
61	65065.5
62	57500.5
63	50028.5
64	41445.5
65	33574.0
66	26812.0
67	20780.5
68	15927.5
69	12163.5
70	8830.5
71	6406.0
72	5033.0
73	3897.5
74	2859.5
75	1976.5
76	1310.0
77	936.5
78	659.0
79	399.0
80	214.0
81	108.0
82	56.5
83	26.0
84	16.0
85	9.0
86	5.5
87	6.5
88	5.0
89	3.5
90	1.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.988119834138528E-4
2	5.095930899177007E-4
3	1.329373278046176E-4
4	0.012473952592333283
5	6.425304177223182E-4
6	0.0037887138424316006
7	7.089990816246271E-4
8	0.1398500688504577
9	0.0011742797289407887
10-11	0.0
12-13	8.862488520307838E-5
14-15	1.5509354910538716E-4
16-17	0.0
18-19	1.7724977040615677E-4
20-21	0.0
22-23	1.4401543845500236E-4
24-25	4.4312442601539194E-4
26-27	0.006325601181369721
28-29	0.008297504877138213
30-31	0.01480035582891409
32-33	0.017780367593867603
34-35	0.026543153118321974
36-37	0.038042231973421395
38-39	0.043215709647151095
40-41	0.054681554170299365
42-43	0.07140950125238041
44-45	0.11073679406124644
46-47	0.11771600377098886
48-49	0.11560008463676535
50-51	0.11591027173497613
52-53	0.11016073230742643
54-55	0.11808158142245156
56-57	0.10011288594752742
58-59	0.09326661356558962
60-61	0.09498372071639925
62-63	0.09339955089339423
64-65	0.0
66-67	2.2156221300769596E-5
68-69	8.862488520307838E-5
70-71	0.0
72-73	0.0
74-75	0.0
76-77	6.64686639023088E-5
78-79	2.2156221300769596E-5
80-81	2.658746556092352E-4
82-83	1.66171659755772E-4
84-85	0.0
86-87	6.64686639023088E-4
88-89	4.3204631536500717E-4
90-91	1.1078110650384799E-4
92-93	7.089990816246271E-4
94-95	7.643896348765509E-4
96-97	1.2185921715423279E-4
98-99	6.64686639023088E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4513405.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.701616611040485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.91960471196293	31.252537230204712
2	16.590891920376443	15.496429474012597
3	6.4882574777221524	9.090363395948895
4	3.1743350886350523	5.929863212176298
5	1.8397396147109082	4.295940707518609
6	1.1529124012439247	3.230572376940471
7	0.7398081292752194	2.418516493339966
8	0.5349094506646673	1.9984908869250855
9	0.40963244771461077	1.721744777214886
>10	1.985183225747405	16.472441219781413
>50	0.11825079785126638	3.774032819680623
>100	0.0452862720315188	3.3575318317476435
>500	8.556927496168344E-4	0.28302141121023955
>1k	2.376923674194203E-4	0.2359991347381172
>5k	4.753847348383756E-5	0.18977616215808496
>10k+	4.753847348383756E-5	0.2527388664023911
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11384	0.2522264232879611	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8548	0.1893913796789785	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4759	0.1054414571703625	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.2156221300769596E-5	0.0
12-13	0.0	0.0	0.0	2.2156221300769596E-5	0.0
14-15	0.0	0.0	0.0	6.64686639023088E-5	0.0
16-17	0.0	0.0	0.0	1.2185921715423279E-4	0.0
18-19	0.0	0.0	0.0	1.329373278046176E-4	0.0
20-21	0.0	0.0	0.0	1.440154384550024E-4	0.0
22-23	0.0	1.1078110650384798E-5	0.0	2.1048410235731117E-4	0.0
24-25	0.0	2.2156221300769596E-5	0.0	3.212652088611591E-4	0.0
26-27	0.0	2.2156221300769596E-5	0.0	4.2096820471462234E-4	0.0
28-29	0.0	4.431244260153919E-5	0.0	0.0012850608354446366	0.0
30-31	0.0	6.64686639023088E-5	0.0	0.004907603018120465	0.0
32-33	0.0	7.754677455269358E-5	0.0	0.012274546600626355	0.0
34-35	0.0	8.862488520307838E-5	0.0	0.023840094119628084	0.0
36-37	0.0	8.862488520307838E-5	0.0	0.04434567693349034	0.0
38-39	0.0	1.1078110650384799E-4	0.0	0.08030522410463939	0.0
40-41	0.0	1.1078110650384799E-4	0.0	0.12517157223869785	0.0
42-43	0.0	1.1078110650384799E-4	0.0	0.17002684226210588	0.0
44-45	0.0	1.1078110650384799E-4	0.0	0.22099722936452632	0.0
46-47	0.0	1.1078110650384799E-4	0.0	0.28482930293204356	0.0
48-49	0.0	1.1078110650384799E-4	0.0	0.36076975144043133	0.0
50-51	0.0	1.1078110650384799E-4	0.0	0.4449633923833558	0.0
52-53	0.0	1.1078110650384799E-4	0.0	0.5330232939432645	0.0
54-55	0.0	1.1078110650384799E-4	0.0	0.6251820964438157	0.0
56-57	0.0	1.329373278046176E-4	0.0	0.733293378280921	0.0
58-59	0.0	1.329373278046176E-4	0.0	0.862619242013513	0.0
60-61	0.0	1.329373278046176E-4	0.0	1.0030121382858397	0.0
62-63	0.0	1.329373278046176E-4	0.0	1.1442802052995464	0.0
64-65	0.0	1.329373278046176E-4	0.0	1.2893923767089372	0.0
66-67	0.0	1.329373278046176E-4	0.0	1.454821803051133	0.0
68-69	0.0	1.5509354910538716E-4	0.0	1.645088353471492	0.0
70-71	0.0	1.8832788105654157E-4	0.0	1.8544535666531146	0.0
72-73	0.0	2.2156221300769597E-4	0.0	2.0740881884076434	0.0
74-75	0.0	2.2156221300769597E-4	0.0	2.2924820617693293	0.0
76-77	0.0	2.2156221300769597E-4	0.0	2.5254768849682225	0.0
78-79	0.0	2.2156221300769597E-4	0.0	2.7993388583563847	0.0
80-81	0.0	2.2156221300769597E-4	0.0	3.096797207429867	0.0
82-83	0.0	2.2156221300769597E-4	0.0	3.4063750095548704	0.0
84-85	0.0	2.2156221300769597E-4	0.0	3.7141692358651617	0.0
86-87	0.0	2.2156221300769597E-4	0.0	4.034171540112177	0.0
88	0.0	2.2156221300769597E-4	0.0	4.291815159508176	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7435	0.0	51.94134	1
GTATCAA	13580	0.0	43.210075	1
TCAACGC	16495	0.0	35.3135	4
ATCAACG	16530	0.0	35.153725	3
CAACGCA	16885	0.0	34.550514	5
TATCAAC	17175	0.0	33.888336	2
AACGCAG	17220	0.0	33.796024	6
ACGCAGA	19830	0.0	29.180464	7
CGCAGAG	20535	0.0	28.017975	8
GTACATG	15875	0.0	26.427742	1
GCAGAGT	22390	0.0	25.490147	9
TACATGG	16095	0.0	25.199593	2
ACATGGG	16325	0.0	23.835793	3
GAGTACT	14430	0.0	21.35465	12-13
CAGAGTA	22015	0.0	21.123539	10-11
AGAGTAC	20760	0.0	20.975029	10-11
CATGGGG	11150	0.0	20.4241	4
GTACTTT	15485	0.0	19.687405	14-15
GTGGTAT	3415	0.0	19.672869	1
AGTACTT	14870	0.0	19.553957	12-13
>>END_MODULE