FastQCFastQC Report
Wed 25 May 2016
SRR1033004_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033004_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051939
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71130.6761798925603101No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59240.56315052488785No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34690.3297719734699446No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25710.24440580680058444No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18970.18033365052536318No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16040.15248032442945836No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14920.14183331923238895No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14920.14183331923238895No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT14310.1360345039018422No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA14300.1359394413554398No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG12030.11436024332209377No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC11680.11103305419800959No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGT300.004161831531.33322334-35
GCCGTAT3450.025.88396694
GTACATG50400.023.7808071
CATGGGG19050.023.1926214
TCTATAC3750.022.5609933
TACATGG50700.022.0640872
GGTGTAC1100.00305859221.3645761
TCGCCGT5200.020.78838794
ACATGGG53000.019.8650253
CCGTATC2702.5949703E-819.14807994
CGCCGTA3750.018.79993292-93
ACAACAC12600.018.6516135
GTACAAA10700.018.4494111
CGGTTCG650.00804124418.07685990-91
CAACACA18450.017.8319156
GTCCTAT2654.4732406E-717.736631
GTATTAG4002.7284841E-1117.6257761
CTATACT4600.017.3703294
CTGTCGC2505.04297E-616.9199399
ATGGGGA12100.016.7032155
Produced by FastQC (version 0.11.5)