##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033004_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1051939
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30425528476461	33.0	31.0	34.0	28.0	34.0
2	31.319867406760277	34.0	31.0	34.0	28.0	34.0
3	31.4312436367508	34.0	31.0	34.0	28.0	34.0
4	34.52634706004816	37.0	35.0	37.0	32.0	37.0
5	34.79260869689212	37.0	35.0	37.0	32.0	37.0
6	34.745372117584765	37.0	37.0	37.0	32.0	37.0
7	34.74316096275545	37.0	37.0	37.0	32.0	37.0
8	34.62108924566919	37.0	37.0	37.0	32.0	37.0
9	36.14311095985604	39.0	38.0	39.0	32.0	39.0
10-11	36.253269913939874	39.0	38.0	39.0	32.0	39.0
12-13	36.21363453584286	39.0	38.0	39.0	32.0	39.0
14-15	37.69551894168768	41.0	39.0	41.0	32.0	41.0
16-17	37.80576297675056	41.0	39.0	41.0	32.0	41.0
18-19	37.865295421122326	41.0	39.0	41.0	32.0	41.0
20-21	37.95898478904195	41.0	39.0	41.0	32.0	41.0
22-23	37.98187489959018	41.0	39.0	41.0	32.0	41.0
24-25	38.04071909112601	41.0	39.0	41.0	32.0	41.0
26-27	37.96583261957205	41.0	39.0	41.0	31.5	41.0
28-29	37.882731793383456	41.0	39.0	41.0	31.0	41.0
30-31	37.770374993226795	41.0	39.0	41.0	31.0	41.0
32-33	37.6433391099674	41.0	39.0	41.0	30.5	41.0
34-35	37.47398138104966	41.0	38.5	41.0	30.0	41.0
36-37	37.31053321532902	41.0	38.0	41.0	30.0	41.0
38-39	37.16791135227423	41.0	38.0	41.0	30.0	41.0
40-41	37.08052415586835	41.0	38.0	41.0	30.0	41.0
42-43	36.96547946221216	41.0	38.0	41.0	30.0	41.0
44-45	36.87909802754723	40.5	38.0	41.0	30.0	41.0
46-47	36.78258387606125	40.0	38.0	41.0	30.0	41.0
48-49	36.652837284291195	40.0	38.0	41.0	30.0	41.0
50-51	36.549546123872204	40.0	38.0	41.0	30.0	41.0
52-53	36.438774491676796	40.0	37.5	41.0	29.5	41.0
54-55	36.31699033879341	40.0	37.0	41.0	29.0	41.0
56-57	36.16307884772786	40.0	37.0	41.0	29.0	41.0
58-59	35.9983207201178	40.0	36.5	41.0	28.5	41.0
60-61	35.7721849841103	40.0	36.0	41.0	28.0	41.0
62-63	35.55924345423071	39.5	35.5	41.0	28.0	41.0
64-65	35.31321255319938	39.0	35.0	41.0	27.5	41.0
66-67	35.029661415728484	39.0	35.0	41.0	27.0	41.0
68-69	34.710878672622655	38.5	35.0	40.0	26.0	41.0
70-71	34.34303272338035	37.5	35.0	40.0	26.0	41.0
72-73	34.035409847909435	37.0	34.0	40.0	26.0	41.0
74-75	33.747284300705644	36.5	34.0	39.0	26.0	41.0
76-77	33.37917360227162	36.0	34.0	39.0	25.0	41.0
78-79	32.96381586765012	35.5	34.0	38.5	24.5	40.0
80-81	32.59244547449995	35.0	34.0	37.0	24.0	39.0
82-83	32.233448422389515	35.0	34.0	37.0	22.5	39.0
84-85	32.02903162635856	35.0	34.0	36.5	23.0	38.5
86-87	31.77785594031593	35.0	34.0	36.0	22.0	37.0
88-89	31.498439072988074	35.0	34.0	36.0	20.0	37.0
90-91	31.2478537253586	35.0	33.0	35.5	19.0	36.5
92-93	31.02414778803714	35.0	33.0	35.0	17.5	36.0
94-95	30.82488005483208	35.0	33.0	35.0	9.0	36.0
96-97	30.63233752147225	35.0	33.0	35.0	2.0	36.0
98-99	30.454219303590797	35.0	33.0	35.0	2.0	36.0
100	30.306885665423565	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.30547313247973307
1101	2	0.348310727814777
1101	3	0.28326186469417536
1101	4	0.11857460193395752
1101	5	0.14271513913263334
1101	6	0.3672617076915188
1101	7	0.035827786102743175
1101	8	0.21290984388888745
1101	9	0.2656969647521805
1101	10-11	0.09055509499570746
1101	12-13	-0.028740925436622433
1101	14-15	-0.06424025206739259
1101	16-17	-0.06330303762017309
1101	18-19	-0.14346962616696857
1101	20-21	-0.09949673417026617
1101	22-23	-0.041509424484949875
1101	24-25	0.048420036630005825
1101	26-27	0.06423377430741084
1101	28-29	0.09997384685004107
1101	30-31	0.09316906117369683
1101	32-33	0.03704703670365461
1101	34-35	0.08515824843056663
1101	36-37	0.04718287224164186
1101	38-39	-0.03556061562752433
1101	40-41	-0.06691614259229794
1101	42-43	-0.13503713744403
1101	44-45	-0.1156733635192424
1101	46-47	-0.08662671200932692
1101	48-49	-0.06717200614795615
1101	50-51	-0.09318730420128674
1101	52-53	-0.12690965646331165
1101	54-55	-0.07617976668047532
1101	56-57	-0.036462382150943995
1101	58-59	-0.23642582869931772
1101	60-61	-0.21601231706843294
1101	62-63	-0.1959578022430648
1101	64-65	-0.1701399749228969
1101	66-67	-0.02728002987618794
1101	68-69	-0.18217018551624165
1101	70-71	-0.16192914694617855
1101	72-73	-0.2702123068307145
1101	74-75	-0.290789944373671
1101	76-77	-0.22944383316249883
1101	78-79	-0.3012238264441933
1101	80-81	-0.33967247215571916
1101	82-83	-0.3632920323404747
1101	84-85	-0.3275803727258193
1101	86-87	-0.39213003507441613
1101	88-89	-0.33106999864186903
1101	90-91	-0.3162536064756871
1101	92-93	-0.37840246048754267
1101	94-95	-0.5608085128881122
1101	96-97	-0.48730316947093755
1101	98-99	-0.41964055522528554
1101	100	-0.44916024717866065
1102	1	0.5726339463229948
1102	2	0.4790285444269031
1102	3	0.4702867523595984
1102	4	0.4067793373920523
1102	5	0.5331986999914662
1102	6	0.42091023837131303
1102	7	0.42788911890765036
1102	8	0.43448236730642975
1102	9	0.4773535181827384
1102	10-11	0.5615307321831366
1102	12-13	0.49753251218889716
1102	14-15	0.6739170859646251
1102	16-17	0.5698478468640005
1102	18-19	0.45828675641862304
1102	20-21	0.5219726931510067
1102	22-23	0.5147258809740549
1102	24-25	0.5702121413435108
1102	26-27	0.5720977303054582
1102	28-29	0.6361961916569356
1102	30-31	0.6622595126007056
1102	32-33	0.6521984139068309
1102	34-35	0.6508427797316116
1102	36-37	0.6448333523748815
1102	38-39	0.579868451580424
1102	40-41	0.619405817143992
1102	42-43	0.7096495307579289
1102	44-45	0.5879853428791364
1102	46-47	0.5530497536958308
1102	48-49	0.5533194810600932
1102	50-51	0.6161920305209421
1102	52-53	0.5082806562525874
1102	54-55	0.5446030674178175
1102	56-57	0.6810861801271244
1102	58-59	0.6841401019237097
1102	60-61	0.6778372643881951
1102	62-63	0.7192613238233818
1102	64-65	0.7480365363052073
1102	66-67	0.7683320931536102
1102	68-69	0.7280941689415243
1102	70-71	0.8057902602914382
1102	72-73	0.749348637690197
1102	74-75	0.7218290178101512
1102	76-77	0.6584933880094255
1102	78-79	0.643372063636626
1102	80-81	0.6392621836639876
1102	82-83	0.5484274498761152
1102	84-85	0.5450423919633707
1102	86-87	0.6829003581903308
1102	88-89	0.59949812779411
1102	90-91	0.5867867911271318
1102	92-93	0.6536025845266238
1102	94-95	0.7661065305729196
1102	96-97	0.8609059040566613
1102	98-99	0.8635665634932188
1102	100	0.939589692570376
1103	1	0.4300247981644567
1103	2	0.4236799899107595
1103	3	0.36250256466420083
1103	4	0.39636254367891866
1103	5	0.3440092361072189
1103	6	0.25819217581972964
1103	7	0.3065018121707368
1103	8	0.3967749766578592
1103	9	0.41288912574942316
1103	10-11	0.36473982423795803
1103	12-13	0.382870028908215
1103	14-15	0.3786016448389802
1103	16-17	0.43656543375362133
1103	18-19	0.48198956119801295
1103	20-21	0.3749916220578413
1103	22-23	0.4000608567640853
1103	24-25	0.40036118209188487
1103	26-27	0.3987717961582149
1103	28-29	0.550002788315922
1103	30-31	0.48497836792223126
1103	32-33	0.54482738131383
1103	34-35	0.6388048731321021
1103	36-37	0.494693906175506
1103	38-39	0.48083420798457865
1103	40-41	0.49432399922088877
1103	42-43	0.5238323882260261
1103	44-45	0.46285982097226963
1103	46-47	0.39087646638380136
1103	48-49	0.37996356394724273
1103	50-51	0.3882493294927798
1103	52-53	0.4797866752058866
1103	54-55	0.39376994752255
1103	56-57	0.4135461452452489
1103	58-59	0.44307146837449807
1103	60-61	0.4521890225079872
1103	62-63	0.4905314429014922
1103	64-65	0.4222375664995752
1103	66-67	0.42354221784209045
1103	68-69	0.47235217693427245
1103	70-71	0.4732939090658945
1103	72-73	0.4143484547432905
1103	74-75	0.4119835266383518
1103	76-77	0.4696992927225452
1103	78-79	0.5310797591967074
1103	80-81	0.40795122677034357
1103	82-83	0.5084047644594278
1103	84-85	0.579894794462831
1103	86-87	0.5510896513241264
1103	88-89	0.5530814479146109
1103	90-91	0.6085711942933294
1103	92-93	0.5056712058793735
1103	94-95	0.5450461906169082
1103	96-97	0.5464394788799147
1103	98-99	0.5442169804088977
1103	100	0.5819234039939865
1104	1	0.2519496846238951
1104	2	0.2900352698868254
1104	3	0.36553484553771654
1104	4	0.2746293843957872
1104	5	0.2641501079899484
1104	6	0.1338210681533596
1104	7	0.29249620992983694
1104	8	0.2341196232543652
1104	9	0.30168736349111924
1104	10-11	0.23158462040885297
1104	12-13	0.24994258608641928
1104	14-15	0.333508364808516
1104	16-17	0.3429692574190142
1104	18-19	0.26127214801915244
1104	20-21	0.23304212381194134
1104	22-23	0.2461895995233192
1104	24-25	0.34575972986050374
1104	26-27	0.366388940103171
1104	28-29	0.35051814008770066
1104	30-31	0.40374247550700915
1104	32-33	0.4783585607758951
1104	34-35	0.49715112425391794
1104	36-37	0.44753038057108796
1104	38-39	0.36819109248595083
1104	40-41	0.43855314719075267
1104	42-43	0.48242067791082377
1104	44-45	0.48014246218409085
1104	46-47	0.39444585530442566
1104	48-49	0.41299570884403636
1104	50-51	0.36881145725650555
1104	52-53	0.383535553397941
1104	54-55	0.40772455849315037
1104	56-57	0.3910997880616094
1104	58-59	0.3202878889232963
1104	60-61	0.4334344332936624
1104	62-63	0.38055007171224275
1104	64-65	0.4293899420128895
1104	66-67	0.36784615300839363
1104	68-69	0.398860158767782
1104	70-71	0.4776417647130131
1104	72-73	0.4534077677888959
1104	74-75	0.48083873862606197
1104	76-77	0.4219986590710221
1104	78-79	0.4524342233124514
1104	80-81	0.4509130717024945
1104	82-83	0.4353240758733108
1104	84-85	0.4718001002744714
1104	86-87	0.4440453773038442
1104	88-89	0.3692885132650261
1104	90-91	0.2860922026966932
1104	92-93	0.327545101068516
1104	94-95	0.28842072489663906
1104	96-97	0.25634674275633884
1104	98-99	0.28183290056336574
1104	100	0.22242135074550262
1105	1	0.001455228332542191
1105	2	-0.08691529000611808
1105	3	0.0044321965036679956
1105	4	-0.1745729970934562
1105	5	-0.1624080620661985
1105	6	-0.23745233281318434
1105	7	-0.08550170456442885
1105	8	-0.13215537451380044
1105	9	-0.11407467901601365
1105	10-11	-0.05486272149168059
1105	12-13	-0.062377313558968694
1105	14-15	-6.966902880378711E-4
1105	16-17	0.009971006998185317
1105	18-19	0.01930654505857632
1105	20-21	0.0712138734003176
1105	22-23	0.08241435556240617
1105	24-25	0.03406635626507182
1105	26-27	0.16377368277882454
1105	28-29	0.08911113238347212
1105	30-31	0.06469064433910177
1105	32-33	0.10992857109298626
1105	34-35	0.06993996817875114
1105	36-37	0.05058666805796008
1105	38-39	-0.034455318575872695
1105	40-41	-0.013202966442619868
1105	42-43	0.019825410517881892
1105	44-45	-0.032480783750045816
1105	46-47	-0.04105463385987207
1105	48-49	-0.01002809960603912
1105	50-51	-0.0501487902063289
1105	52-53	-0.04797592286585939
1105	54-55	-0.061691168576182065
1105	56-57	-0.04135688431428264
1105	58-59	-0.07139719234792352
1105	60-61	-0.05996783094341396
1105	62-63	-0.13653547543849953
1105	64-65	-0.07950267837076552
1105	66-67	-0.04810364023571623
1105	68-69	-0.047297255499621826
1105	70-71	-0.14848071319176626
1105	72-73	-0.17556249304657712
1105	74-75	-0.11729185305566148
1105	76-77	-0.1438641153878777
1105	78-79	-0.12228274705330477
1105	80-81	-0.08370070938567409
1105	82-83	-0.06540345823243854
1105	84-85	-0.0421277371775588
1105	86-87	-0.026371226855943064
1105	88-89	0.12631950226043998
1105	90-91	0.06381994940253932
1105	92-93	-0.02097916893544749
1105	94-95	0.00976987040069588
1105	96-97	0.005166167962979529
1105	98-99	-0.056208159786532264
1105	100	-0.002433165442013774
1106	1	0.06164593536531626
1106	2	0.09300051554866684
1106	3	0.1531922167308153
1106	4	0.1630377668228391
1106	5	-0.015197083076408546
1106	6	-0.11808611684782022
1106	7	0.0873055806234575
1106	8	0.001932280737044323
1106	9	0.016250470287239693
1106	10-11	0.1420827228121837
1106	12-13	0.13190382248995292
1106	14-15	0.1401260938468738
1106	16-17	0.087385729717667
1106	18-19	0.12388837860279267
1106	20-21	0.1598203123764108
1106	22-23	0.060278641627220964
1106	24-25	0.16717871296275177
1106	26-27	0.16200551408267927
1106	28-29	0.09669908741434341
1106	30-31	0.062100374281044424
1106	32-33	0.06806114427238441
1106	34-35	0.06900158204075524
1106	36-37	0.22752601007815798
1106	38-39	0.3074633162195184
1106	40-41	0.24420736092569229
1106	42-43	0.2666026152879084
1106	44-45	0.2647219806271295
1106	46-47	0.23048034652106253
1106	48-49	0.2842037487671476
1106	50-51	0.2945086177740066
1106	52-53	0.24851185016333233
1106	54-55	0.27372234648856164
1106	56-57	0.307473973611323
1106	58-59	0.17105272518590908
1106	60-61	0.26235884606442283
1106	62-63	0.26869733948520746
1106	64-65	0.32675493614840434
1106	66-67	0.22492746647530026
1106	68-69	0.25432795760023197
1106	70-71	0.1636816075536558
1106	72-73	0.10495884681008505
1106	74-75	0.24662892820733617
1106	76-77	0.2362767918894093
1106	78-79	0.2376864854885028
1106	80-81	0.13559360204843074
1106	82-83	0.07126379874838307
1106	84-85	0.0805234703704869
1106	86-87	0.058740267840832416
1106	88-89	0.02679594979253963
1106	90-91	0.02074998864065236
1106	92-93	-0.031832059092209875
1106	94-95	-0.09830925445501038
1106	96-97	-0.10086613146711798
1106	98-99	-0.0662536708741257
1106	100	0.01563727539463855
1107	1	0.26196106394258933
1107	2	0.27361048579988534
1107	3	0.4552870600084731
1107	4	0.2690512700422829
1107	5	0.23358850921296437
1107	6	0.27794671105028357
1107	7	0.2559803567057557
1107	8	0.3637937043484385
1107	9	0.36176169223237054
1107	10-11	0.4144492341864776
1107	12-13	0.42174954580224977
1107	14-15	0.41309960861300965
1107	16-17	0.5192622281821144
1107	18-19	0.39669771378573415
1107	20-21	0.4402905595879645
1107	22-23	0.4253416673426784
1107	24-25	0.426126600478689
1107	26-27	0.3550636645265115
1107	28-29	0.3799988765267628
1107	30-31	0.3499579008825435
1107	32-33	0.21933508963406467
1107	34-35	0.2681872994193313
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2216	40-41	-0.6121653988639295
2216	42-43	-0.6210380599532499
2216	44-45	-0.6977119658820072
2216	46-47	-0.69842553348591
2216	48-49	-0.7974251240861179
2216	50-51	-0.7369135263472302
2216	52-53	-0.8068640315884608
2216	54-55	-0.7879873484190867
2216	56-57	-0.9042079912930845
2216	58-59	-0.8977471265053154
2216	60-61	-0.9883238105322079
2216	62-63	-0.8887679512747795
2216	64-65	-0.9247471576226332
2216	66-67	-1.0215896139603942
2216	68-69	-1.0198409212352217
2216	70-71	-0.9036970035782304
2216	72-73	-0.8317837190564674
2216	74-75	-0.8287438689710598
2216	76-77	-0.911004733073824
2216	78-79	-0.7672232829503898
2216	80-81	-0.7331124661486825
2216	82-83	-0.816006104539337
2216	84-85	-0.8214043880008539
2216	86-87	-0.8677083911486747
2216	88-89	-0.8328095363860832
2216	90-91	-0.8498781738260099
2216	92-93	-0.8577904578558879
2216	94-95	-0.9171732901986758
2216	96-97	-0.912396671859451
2216	98-99	-0.9613404751314576
2216	100	-0.9853401293263175
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	17814.0
3	2657.0
4	1732.0
5	1703.0
6	3219.0
7	7503.0
8	9266.0
9	5874.0
10	3599.0
11	3393.0
12	3361.0
13	2760.0
14	2752.0
15	2518.0
16	2446.0
17	2393.0
18	2191.0
19	1968.0
20	1795.0
21	1801.0
22	2203.0
23	3275.0
24	6482.0
25	7482.0
26	8001.0
27	5995.0
28	5683.0
29	6957.0
30	8399.0
31	10011.0
32	13550.0
33	18327.0
34	26234.0
35	43331.0
36	73651.0
37	169142.0
38	351278.0
39	210703.0
40	490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.06577683064892	22.48028312387621	13.93176866800491	21.522171377469963
2	14.428842844136112	24.344521897473417	39.17560886040664	22.051026397983833
3	18.08005282180917	28.80853140718936	31.62953188193869	21.481883889062782
4	11.161991060436247	19.904229833177617	40.7997465411043	28.134032565281842
5	11.071331731798397	41.69797588183526	35.55692980995647	11.673762576409866
6	26.163734911904648	42.375867800360965	18.111993697892732	13.348403589841656
7	22.880490004680844	37.54200393495527	23.321459967348243	16.256046093015645
8	22.19756006366706	37.71455047269842	21.83041843109982	18.257471032534706
9	22.689766386482667	20.399393013952118	22.895646920592334	34.015193678972885
10-11	20.946118545953766	30.21707372648225	29.65955963046139	19.17724809710259
12-13	22.574892399258687	28.719027921012874	28.86221016298991	19.843869516738526
14-15	20.065900883244982	30.96656531484392	27.120539536724497	21.846994265186602
16-17	18.561320474248134	33.7308867774197	28.048946974111377	19.658845774220783
18-19	18.64604788112011	33.214399535744356	29.377440790756747	18.762111792378786
20-21	20.76950848664536	31.76854145192201	27.954533670931355	19.507416390501277
22-23	19.914397212914814	31.775111451061427	28.28978037053316	20.020710965490604
24-25	20.700715566314393	31.619059760228442	27.874977821392406	19.805246852064762
26-27	19.911447595870726	31.197740714983603	28.91638419261575	19.974427496529923
28-29	19.39097647441029	32.50349965681245	28.692476843759053	19.41304702501821
30-31	20.109369366112432	32.58871276936821	27.85213254090648	19.449785323612883
32-33	19.39306616841208	32.34491064905017	28.768066168412084	19.49395701412567
34-35	19.91589335837221	31.901827067193512	28.41122544451849	19.771054129915793
36-37	19.94950515912106	31.899459330391604	28.617484932645798	19.533550577841535
38-39	19.771608831607175	31.993409305170672	28.850795154773827	19.384186708448322
40-41	19.30922191737034	32.005911631853934	28.88613211460232	19.798734336173403
42-43	19.107524600442012	31.949749738131235	28.46886925018481	20.473856411241947
44-45	19.560904069721808	31.789430320766556	28.56849284767311	20.081172761838527
46-47	19.817802979848913	32.37883660304835	28.36950316823017	19.433857248872567
48-49	19.49019339198079	31.636562749161296	28.67726393067346	20.19597992818446
50-51	18.71152816926535	31.604238769757732	29.066542976415597	20.617690084561318
52-53	19.253959267945746	31.664961965338268	28.259967745465097	20.821111021250886
54-55	19.125167814289643	31.50874442085873	28.470960872986346	20.895126891865274
56-57	19.87355381336581	30.77175940021312	28.032758030141576	21.321928756279497
58-59	20.115503544074972	30.437895437895442	28.22177822177822	21.224822796251367
60-61	19.871312458792108	30.57680104046056	28.535743949278498	21.016142551468832
62-63	19.61614190552349	30.472125666763873	28.37463507269528	21.537097355017362
64-65	19.550886239736652	30.814265462814085	28.419087215884765	21.2157610815645
66-67	19.85422557455408	30.187208156213252	28.033700702898745	21.924865566333928
68-69	20.319442250937605	30.538340491036852	27.959854702106973	21.18236255591857
70-71	20.761706058694646	30.14577095826186	27.789289516421352	21.303233466622142
72-73	20.40428103687516	29.636081421329358	28.983479695804544	20.97615784599094
74-75	20.337435044610153	30.539926515498912	28.252288541783603	20.870349898107328
76-77	20.638785809389844	29.80748327828801	28.34668517420026	21.207045738121888
78-79	20.95450162614814	29.47392906597685	29.025733431579287	20.54583587629572
80-81	21.407117550128817	29.367315135116623	28.50140615664471	20.72416115810985
82-83	20.87931690609837	29.027399866806203	29.300684996670157	20.79259823042527
84-85	20.70330914444595	29.304848956215025	28.816361867658006	21.175480031681023
86-87	21.03475554384294	29.328364563359187	28.471594241643384	21.165285651154484
88-89	20.99872945713904	29.09214931994324	28.351657342534264	21.557463880383455
90-91	21.310747868045222	28.59164456384829	28.3467218698033	21.750885698303186
92-93	21.565491788345906	29.13732800524374	28.219626250465556	21.077553955944797
94-95	21.019592733707206	28.991728146657724	28.499412550955856	21.489266568679216
96-97	20.44622998167742	29.315842848200376	28.57589981372995	21.66202735639226
98-99	20.92307882572417	29.559432516431738	27.733842481736964	21.783646176107126
100	20.767331795371845	29.434204580588393	27.554118010797442	22.244345613242324
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6873.0
1	4081.5
2	1124.5
3	852.0
4	623.0
5	399.5
6	285.0
7	293.0
8	327.5
9	347.5
10	278.0
11	213.5
12	208.5
13	206.5
14	232.0
15	261.5
16	313.0
17	388.0
18	530.0
19	717.5
20	951.0
21	1355.5
22	2096.0
23	3499.5
24	5071.0
25	6777.0
26	9484.0
27	13001.0
28	18231.0
29	22733.5
30	24861.5
31	28087.0
32	32369.0
33	36139.0
34	39671.0
35	42244.5
36	43282.0
37	43331.0
38	42220.0
39	41401.0
40	40617.0
41	38174.5
42	35483.0
43	33625.0
44	32329.0
45	30902.0
46	30710.0
47	34333.5
48	42690.0
49	42129.5
50	33612.0
51	27600.0
52	22457.5
53	19611.5
54	17293.0
55	16189.5
56	15091.5
57	13191.0
58	11278.0
59	9201.0
60	7446.5
61	5950.0
62	4604.0
63	3479.0
64	2618.0
65	2018.5
66	1608.5
67	1227.0
68	942.0
69	758.0
70	571.5
71	446.0
72	375.5
73	306.5
74	256.5
75	198.5
76	151.5
77	119.5
78	105.0
79	78.5
80	58.5
81	45.5
82	37.0
83	27.0
84	19.0
85	12.0
86	8.0
87	8.5
88	6.0
89	2.5
90	2.5
91	2.0
92	2.5
93	2.5
94	0.5
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07690560003954602
2	0.07947228879241097
3	0.08156366481326388
4	0.0837501033805192
5	0.08213404009167832
6	0.08194391499887352
7	0.08051797680283743
8	0.08042291425643502
9	0.08023278916363022
10-11	0.0802803204368314
12-13	0.07899697606039893
14-15	0.07747597531796045
16-17	0.07619263094152798
18-19	0.07547966184350995
20-21	0.0748617552918943
22-23	0.0765253498539364
24-25	0.07733338149835685
26-27	0.07700066258594843
28-29	0.07267531672463898
30-31	0.07814141314277728
32-33	0.0763352247611316
34-35	0.07353087964226063
36-37	0.07058394070378606
38-39	0.07224753526582815
40-41	0.07519447420430272
42-43	0.07985253897802058
44-45	0.08056550807603863
46-47	0.0835599782877144
48-49	0.08679210486539618
50-51	0.08807544924182865
52-53	0.08783779287582265
54-55	0.08802791796862747
56-57	0.0854612292157625
58-59	0.08451060375173845
60-61	0.08232416518448313
62-63	0.08303713428250117
64-65	0.08260935282369035
66-67	0.0820389775452759
68-69	0.08146860226686148
70-71	0.07966241388521578
72-73	0.07970994515841698
74-75	0.07918710115320375
76-77	0.07942475751920976
78-79	0.07942475751920976
80-81	0.08051797680283743
82-83	0.07975747643161818
84-85	0.07951982006561217
86-87	0.07990007025122178
88-89	0.07733338149835685
90-91	0.07519447420430272
92-93	0.07419631746707746
94-95	0.07452903637948588
96-97	0.07386359855466905
98-99	0.07338828582265701
100	0.07262778545143778
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1051939.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.706835507519386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.44443141406786	43.116901539689714
2	15.499179103921467	18.198155163109988
3	5.531025215965999	9.741269626249734
4	2.4507134622307305	5.754945284129712
5	1.2146390688997772	3.565380800945286
6	0.6688833363965055	2.3560814402130243
7	0.35591053850065324	1.4626067007405346
8	0.21433092260232828	1.0066152173911804
9	0.1370850259323202	0.7243045261157475
>10	0.41458897688743407	4.033115852581677
>50	0.027337278096473273	1.14069454604075
>100	0.03557005788613444	4.102563782526581
>500	0.0038803683768508903	1.5829540734731276
>1k	0.0021018662041275655	1.9769603311325958
>5k	3.2336403140424084E-4	1.2374511156604404
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7113	0.6761798925603101	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	5924	0.56315052488785	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	3469	0.3297719734699446	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	2571	0.24440580680058444	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	1897	0.18033365052536318	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	1604	0.15248032442945836	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	1492	0.14183331923238895	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	1492	0.14183331923238895	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	1431	0.1360345039018422	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	1430	0.1359394413554398	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	1203	0.11436024332209377	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	1168	0.11103305419800959	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.506254640240546E-5	0.0
10-11	0.0	0.0	0.0	1.425938196036082E-4	0.0
12-13	0.0	0.0	0.0	3.8025018560962186E-4	0.0
14-15	0.0	0.0	0.0	4.753127320120273E-4	0.0
16-17	0.0	0.0	0.0	5.703752784144328E-4	0.0
18-19	0.0	0.0	0.0	0.001093219283627663	0.0
20-21	0.0	0.0	0.0	0.0017111258352432983	0.0
22-23	0.0	0.0	0.0	0.004135220768504638	0.0
24-25	0.0	0.0	0.0	0.009791442279447763	0.0
26-27	0.0	0.0	0.0	0.016778539440024564	0.0
28-29	0.0	0.0	0.0	0.03645648654532249	0.0
30-31	0.0	0.0	0.0	0.08451060375173845	0.0
32-33	0.0	0.0	0.0	0.16626439365780715	0.0
34-35	0.0	0.0	0.0	0.28965557888812943	0.0
36-37	0.0	0.0	0.0	0.47792695203809343	0.0
38-39	0.0	0.0	0.0	0.7325044513037353	0.0
40-41	0.0	0.0	0.0	1.0127013068248254	0.0
42-43	0.0	0.0	0.0	1.258770708187452	0.0
44-45	0.0	0.0	0.0	1.535640374584458	0.0
46-47	0.0	0.0	0.0	1.877532822720709	0.0
48-49	0.0	0.0	0.0	2.2227524599810446	0.0
50-51	0.0	0.0	0.0	2.566593690318545	0.0
52-53	0.0	0.0	0.0	2.8929909433912044	0.0
54-55	0.0	0.0	0.0	3.2630219052625673	0.0
56-57	0.0	0.0	0.0	3.706013371497777	0.0
58-59	0.0	0.0	0.0	4.161553093858104	0.0
60-61	0.0	0.0	0.0	4.617900847862851	0.0
62-63	0.0	0.0	0.0	5.067213973433821	0.0
64-65	0.0	0.0	0.0	5.545235988018317	0.0
66-67	0.0	0.0	0.0	6.099593227363944	0.0
68-69	0.0	0.0	0.0	6.726435658341406	0.0
70-71	0.0	0.0	0.0	7.381464134327181	0.0
72-73	0.0	0.0	0.0	8.025940667662288	0.0
74-75	0.0	0.0	0.0	8.6942779001444	0.0
76-77	0.0	0.0	0.0	9.391751803098849	0.0
78-79	0.0	0.0	0.0	10.213900235660052	0.0
80-81	0.0	0.0	0.0	11.064805088507985	0.0
82-83	0.0	0.0	0.0	11.901165371756347	0.0
84-85	0.0	0.0	0.0	12.748125128928578	0.0
86-87	0.0	0.0	0.0	13.60806092368474	0.0
88	0.0	0.0	0.0	14.302540356427512	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACGT	30	0.0041618315	31.333223	34-35
GCCGTAT	345	0.0	25.883966	94
GTACATG	5040	0.0	23.780807	1
CATGGGG	1905	0.0	23.192621	4
TCTATAC	375	0.0	22.560993	3
TACATGG	5070	0.0	22.064087	2
GGTGTAC	110	0.003058592	21.364576	1
TCGCCGT	520	0.0	20.788387	94
ACATGGG	5300	0.0	19.865025	3
CCGTATC	270	2.5949703E-8	19.148079	94
CGCCGTA	375	0.0	18.799932	92-93
ACAACAC	1260	0.0	18.651613	5
GTACAAA	1070	0.0	18.449411	1
CGGTTCG	65	0.008041244	18.076859	90-91
CAACACA	1845	0.0	17.831915	6
GTCCTAT	265	4.4732406E-7	17.73663	1
GTATTAG	400	2.7284841E-11	17.625776	1
CTATACT	460	0.0	17.370329	4
CTGTCGC	250	5.04297E-6	16.919939	9
ATGGGGA	1210	0.0	16.703215	5
>>END_MODULE