FastQCFastQC Report
Wed 25 May 2016
SRR1033004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033004_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051939
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60150.5718012166104689No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58600.557066521918096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57860.550031893484318No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35010.3328139749548215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31090.29554945676507854No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25770.24497618207899888No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT25270.2402230547588786No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19620.1865127160415195No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC15020.142783944696413No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15000.1425938196036082No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14890.14154813159318172No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA14380.13669994172665906No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT14370.13660487918025666No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13630.12957025074647865No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13210.12557762379757761No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13100.12453193578715116No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC12000.11407505568288656No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG11810.11226886730124086No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG550.002708757334.202765
GCGGTAT650.006164130328.9132771
CATGGGG18000.023.5166344
GTACATG43450.023.14061
TACATGG45150.021.9801082
GAGTACT79200.021.38199212-13
ACATGGG45550.020.9610373
TCGCGCG1150.00397056420.4239759
CTATACT3700.020.3387094
AGTACTT81750.019.47959912-13
GTACTTT90450.018.87834514-15
TATTGCG1250.006391698518.8097258
GTATTAG3759.094947E-1218.7936291
TATCGCG1300.00801454618.0862757
CTTACAC3501.4952093E-917.46953
TGTATCG1350.00995512817.418075
AGAGTAC115350.017.26697710-11
GTACTAG2203.2034302E-517.085121
TAGAGAG4457.2759576E-1216.9092275
TCTATAC3652.6102498E-916.7515743
Produced by FastQC (version 0.11.5)