##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033004_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1051939
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78974541299448	34.0	31.0	34.0	31.0	34.0
2	32.829339914196545	34.0	31.0	34.0	31.0	34.0
3	32.89828592722582	34.0	31.0	34.0	31.0	34.0
4	36.31758400439569	37.0	37.0	37.0	35.0	37.0
5	36.3215500138316	37.0	37.0	37.0	35.0	37.0
6	36.393340298249235	37.0	37.0	37.0	35.0	37.0
7	36.35505766018752	37.0	37.0	37.0	35.0	37.0
8	36.30644837771011	37.0	37.0	37.0	35.0	37.0
9	38.13530917667279	39.0	39.0	39.0	37.0	39.0
10-11	38.14949440984696	39.0	39.0	39.0	37.0	39.0
12-13	38.14726234125743	39.0	39.0	39.0	37.0	39.0
14-15	39.61593305315232	41.0	40.0	41.0	37.0	41.0
16-17	39.65480365306353	41.0	40.0	41.0	37.0	41.0
18-19	39.72877229573198	41.0	40.0	41.0	37.0	41.0
20-21	39.745935838484925	41.0	40.0	41.0	37.0	41.0
22-23	39.67287409250916	41.0	40.0	41.0	37.0	41.0
24-25	39.62765188856008	41.0	40.0	41.0	37.0	41.0
26-27	39.50266793036478	41.0	40.0	41.0	36.0	41.0
28-29	39.41184279696826	41.0	39.5	41.0	36.0	41.0
30-31	39.200543472577785	41.0	39.0	41.0	35.5	41.0
32-33	38.93339442686315	41.0	39.0	41.0	35.0	41.0
34-35	38.747317572596884	41.0	38.5	41.0	35.0	41.0
36-37	38.5787669246981	41.0	38.0	41.0	35.0	41.0
38-39	38.388608084689324	41.0	38.0	41.0	35.0	41.0
40-41	38.2328633124164	41.0	38.0	41.0	35.0	41.0
42-43	38.00471747886522	41.0	38.0	41.0	34.5	41.0
44-45	37.679833146218556	41.0	38.0	41.0	33.5	41.0
46-47	37.24860471947518	41.0	38.0	41.0	33.0	41.0
48-49	36.87094213637863	41.0	38.0	41.0	31.0	41.0
50-51	36.636364370937855	40.0	38.0	41.0	30.5	41.0
52-53	36.50269359725232	40.0	38.0	41.0	30.0	41.0
54-55	36.37159759263608	40.0	37.0	41.0	30.0	41.0
56-57	36.191495419411204	40.0	37.0	41.0	28.5	41.0
58-59	36.05345652171846	40.0	37.0	41.0	28.0	41.0
60-61	35.86876567937875	40.0	36.0	41.0	28.0	41.0
62-63	35.671079786945825	40.0	36.0	41.0	28.0	41.0
64-65	35.40562808299721	39.0	35.0	41.0	27.5	41.0
66-67	35.11477614196261	39.0	35.0	41.0	26.0	41.0
68-69	34.78486917967677	38.5	35.0	41.0	26.0	41.0
70-71	34.39389784008388	37.5	35.0	40.0	25.5	41.0
72-73	34.0222066108396	37.0	35.0	40.0	24.5	41.0
74-75	33.68314607596068	36.5	35.0	39.0	24.0	41.0
76-77	33.296847060523476	36.0	34.0	39.0	22.5	41.0
78-79	32.91440235603015	35.5	34.0	38.5	22.0	40.0
80-81	32.542623669243184	35.0	34.0	37.0	20.5	39.0
82-83	32.19408729973886	35.0	34.0	37.0	20.0	39.0
84-85	31.881678025056587	35.0	34.0	36.5	19.5	38.0
86-87	31.718129093036765	35.0	34.0	36.0	19.0	37.0
88-89	31.489850171920615	35.0	34.0	36.0	18.0	37.0
90-91	31.277111125264867	35.0	34.0	35.5	15.5	36.5
92-93	31.091440663384475	35.0	34.0	35.0	8.5	36.0
94-95	30.970421763999624	35.0	34.0	35.0	4.5	36.0
96-97	30.901129247988713	35.0	34.0	35.0	2.0	36.0
98-99	30.783446568669856	35.0	34.0	35.0	2.0	36.0
100	30.680803734817324	35.0	34.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.10793349656005091
1101	2	0.15617161591478634
1101	3	0.14239226509237568
1101	4	0.04104123797070969
1101	5	0.09170200780001636
1101	6	0.11956910299527834
1101	7	0.05515178338986004
1101	8	0.06113109937207639
1101	9	0.09996419139888246
1101	10-11	0.1762860934697983
1101	12-13	0.13603909110453571
1101	14-15	0.2683839650522586
1101	16-17	0.19089319971990903
1101	18-19	0.27192268933813324
1101	20-21	0.18493059889593866
1101	22-23	0.20210793345560063
1101	24-25	0.20285450245332015
1101	26-27	0.2797630187635036
1101	28-29	0.26410485135220085
1101	30-31	0.24022590372212704
1101	32-33	0.23600008770005587
1101	34-35	0.2725315875094694
1101	36-37	0.2637569983887076
1101	38-39	0.2509427882680981
1101	40-41	0.11106275083778883
1101	42-43	0.05622480085795445
1101	44-45	0.00611746251687606
1101	46-47	0.08548204205339971
1101	48-49	-0.01683632671660007
1101	50-51	-0.25199838610347314
1101	52-53	-0.06534148619272173
1101	54-55	-0.026169569452953567
1101	56-57	-0.1156827510932743
1101	58-59	-0.08347226629403792
1101	60-61	-0.1057813398072085
1101	62-63	-0.023208127331898254
1101	64-65	0.054449267064221374
1101	66-67	0.059812465016541694
1101	68-69	0.006679943862494042
1101	70-71	0.017005190094565137
1101	72-73	-0.05206181467210058
1101	74-75	-0.028901409576860715
1101	76-77	-0.05346857705993813
1101	78-79	-0.03832013625357433
1101	80-81	-0.008198544397231444
1101	82-83	0.06982522982264072
1101	84-85	0.0017678214121339408
1101	86-87	-0.07955919828902935
1101	88-89	0.04954440676501903
1101	90-91	0.01711753450138076
1101	92-93	-0.055288565709894044
1101	94-95	0.09218815315541917
1101	96-97	-0.007460277616882394
1101	98-99	0.03597181456764886
1101	100	-0.015283242016668197
1102	1	0.20863401445056695
1102	2	0.2212742567815411
1102	3	0.22708744876410236
1102	4	0.09580320649025253
1102	5	0.13970997699436793
1102	6	0.13255706886912577
1102	7	0.15121538698671344
1102	8	0.09046930276862497
1102	9	0.16909534570026352
1102	10-11	0.1963448448555738
1102	12-13	0.19636851660489185
1102	14-15	0.29849605862602147
1102	16-17	0.29896870355187133
1102	18-19	0.2827089510468994
1102	20-21	0.330659742689555
1102	22-23	0.31204586157218017
1102	24-25	0.2827759351945005
1102	26-27	0.37042974255700756
1102	28-29	0.38124959827136706
1102	30-31	0.4103395504318925
1102	32-33	0.3311160105573947
1102	34-35	0.29077129530566737
1102	36-37	0.3483769352992496
1102	38-39	0.35570261453109
1102	40-41	0.32595009492168003
1102	42-43	0.4827992255281828
1102	44-45	0.402547927547225
1102	46-47	0.39663409406738737
1102	48-49	0.3510477664929823
1102	50-51	0.3690091171500782
1102	52-53	0.29243624397935264
1102	54-55	0.31427998308320326
1102	56-57	0.3506310680681395
1102	58-59	0.355556970152179
1102	60-61	0.36357207361081834
1102	62-63	0.33960096012036445
1102	64-65	0.3410842640382796
1102	66-67	0.3361715929039093
1102	68-69	0.39878947743969206
1102	70-71	0.48133045961721166
1102	72-73	0.44236564048779314
1102	74-75	0.5143518212137934
1102	76-77	0.4403802010504876
1102	78-79	0.358037224272131
1102	80-81	0.46628985796139233
1102	82-83	0.47003543874620846
1102	84-85	0.4046071146816992
1102	86-87	0.3567539246390048
1102	88-89	0.30702650553375577
1102	90-91	0.35596280466671004
1102	92-93	0.3924350189279835
1102	94-95	0.46437175525268515
1102	96-97	0.33157940232998584
1102	98-99	0.32239446036159336
1102	100	0.4232736268611639
1103	1	0.18678710442385693
1103	2	0.18774313751235638
1103	3	0.22425888627959978
1103	4	0.1754257666899477
1103	5	0.12934400186701822
1103	6	0.05555471495223685
1103	7	0.16401252325776738
1103	8	0.1198099214544115
1103	9	0.1635429070179697
1103	10-11	0.1591685084284009
1103	12-13	0.18450390138288952
1103	14-15	0.18318106470393047
1103	16-17	0.29123868846890844
1103	18-19	0.18752472350973193
1103	20-21	0.16678352456805356
1103	22-23	0.23245125159466085
1103	24-25	0.16849865679402853
1103	26-27	0.20349424557156226
1103	28-29	0.30610822096573287
1103	30-31	0.2832295627503143
1103	32-33	0.28147539959603307
1103	34-35	0.35709209067751857
1103	36-37	0.3298347579057932
1103	38-39	0.4641388046820438
1103	40-41	0.40405013029140946
1103	42-43	0.4099827985219662
1103	44-45	0.3335144360664444
1103	46-47	0.2853215719613047
1103	48-49	0.386355503521024
1103	50-51	0.35517883903012404
1103	52-53	0.3136103159303403
1103	54-55	0.2941227910336721
1103	56-57	0.267183901102662
1103	58-59	0.26866836855769094
1103	60-61	0.2450936227239353
1103	62-63	0.23908992842176957
1103	64-65	0.2525908115048665
1103	66-67	0.3028574689959953
1103	68-69	0.3547607629059826
1103	70-71	0.31018921647263653
1103	72-73	0.2244402990885277
1103	74-75	0.327861332420305
1103	76-77	0.33438686031796294
1103	78-79	0.3988882526370361
1103	80-81	0.282590834084786
1103	82-83	0.279033784093059
1103	84-85	0.38770485305136404
1103	86-87	0.35494884847210884
1103	88-89	0.2894254883489431
1103	90-91	0.2747384426432333
1103	92-93	0.30748108089413506
1103	94-95	0.33178860588263603
1103	96-97	0.36039557321053195
1103	98-99	0.32696559215247945
1103	100	0.324419281058983
1104	1	0.11117724883113311
1104	2	0.18662132956587385
1104	3	0.19656722709165564
1104	4	0.11642546754116267
1104	5	0.0880682682726075
1104	6	0.08643502824259741
1104	7	0.08815790956762726
1104	8	0.02685623512551416
1104	9	0.019727128279285466
1104	10-11	0.0800810482015919
1104	12-13	0.1249630947689866
1104	14-15	0.1723675159834741
1104	16-17	0.17014122035545398
1104	18-19	0.1260088550264129
1104	20-21	0.1645297104210428
1104	22-23	0.22294307939375102
1104	24-25	0.26966873647638323
1104	26-27	0.2664412690411524
1104	28-29	0.2954476460561608
1104	30-31	0.29221422457621316
1104	32-33	0.17791552904586894
1104	34-35	0.2643661653559377
1104	36-37	0.20567781357465975
1104	38-39	0.32044880441009127
1104	40-41	0.2589697680494254
1104	42-43	0.31568634207530977
1104	44-45	0.37133976037092253
1104	46-47	0.4428743989173256
1104	48-49	0.4559415903829489
1104	50-51	0.40376672078476616
1104	52-53	0.36216080408676987
1104	54-55	0.33165576538846864
1104	56-57	0.3097378158724524
1104	58-59	0.3069866084750146
1104	60-61	0.310756781191202
1104	62-63	0.2009892512576954
1104	64-65	0.31029933180619906
1104	66-67	0.3544844304601895
1104	68-69	0.3215790436163246
1104	70-71	0.4006239340489941
1104	72-73	0.38305034504865887
1104	74-75	0.4041612698710182
1104	76-77	0.3654711386895073
1104	78-79	0.39465937662060924
1104	80-81	0.5422451812605544
1104	82-83	0.3562209696468841
1104	84-85	0.4064832381918215
1104	86-87	0.41307889660786756
1104	88-89	0.42452175986726104
1104	90-91	0.4234765786743466
1104	92-93	0.4771630313442188
1104	94-95	0.5395168428084745
1104	96-97	0.5802835283926022
1104	98-99	0.6172316985950594
1104	100	0.6157494053417061
1105	1	0.05595368280039992
1105	2	0.09452130996248798
1105	3	0.07037802404751403
1105	4	0.07393765983473344
1105	5	0.08635182158415233
1105	6	0.11559943622495439
1105	7	0.11712628369226508
1105	8	0.09918720654230384
1105	9	0.14315206986027817
1105	10-11	0.11395164928926249
1105	12-13	0.11555093864504329
1105	14-15	0.20480122570794634
1105	16-17	0.18117226641236783
1105	18-19	0.10456592618854899
1105	20-21	0.17191434265507866
1105	22-23	0.17303083445634826
1105	24-25	0.1807554174943249
1105	26-27	0.16618260558710318
1105	28-29	0.168065213450852
1105	30-31	0.13641539580948603
1105	32-33	0.1581777726487701
1105	34-35	0.06900070946677772
1105	36-37	0.1532914464869961
1105	38-39	0.1185194355010637
1105	40-41	0.06101928822664604
1105	42-43	0.2207201326062318
1105	44-45	0.2306129901529772
1105	46-47	0.3121536385636574
1105	48-49	0.2632920777525811
1105	50-51	0.28685645367253443
1105	52-53	0.34933761282123044
1105	54-55	0.29230657018567996
1105	56-57	0.3559201255194395
1105	58-59	0.3747749784212715
1105	60-61	0.3256802037398785
1105	62-63	0.24814988949975714
1105	64-65	0.24216514156868385
1105	66-67	0.3181309639281409
1105	68-69	0.28271625167083414
1105	70-71	0.16667524153960755
1105	72-73	0.22299652317072827
1105	74-75	0.19049932059500208
1105	76-77	0.13491576464678445
1105	78-79	0.21556542118195665
1105	80-81	0.208498688932103
1105	82-83	0.23815359658341606
1105	84-85	0.25402848934064437
1105	86-87	0.22458382920627784
1105	88-89	0.2124701873167858
1105	90-91	0.24117843602612155
1105	92-93	0.2191233423109793
1105	94-95	0.21734578163946594
1105	96-97	0.33089970054162166
1105	98-99	0.3795551415754339
1105	100	0.261984220770767
1106	1	0.07922002614559887
1106	2	0.12111745186832223
1106	3	0.04257380066979266
1106	4	0.019662754089409873
1106	5	0.0469374701224865
1106	6	-1.9929083013892068E-4
1106	7	0.043775553871938655
1106	8	-0.038250730459083115
1106	9	-0.0639517564422718
1106	10-11	0.06063369257279305
1106	12-13	0.09474983255278602
1106	14-15	0.10558214732346727
1106	16-17	0.10887471394019599
1106	18-19	0.06193230004516437
1106	20-21	0.06569257371825898
1106	22-23	0.11326109964964104
1106	24-25	0.10171782870716584
1106	26-27	0.0756458393280397
1106	28-29	0.04624080132906272
1106	30-31	0.13313296462553836
1106	32-33	0.09482710683840878
1106	34-35	0.1500057513186306
1106	36-37	0.18691524283288175
1106	38-39	0.24891030170528694
1106	40-41	0.2300883912549594
1106	42-43	0.12785660399270427
1106	44-45	0.1948420860975162
1106	46-47	0.09957418619028147
1106	48-49	0.2196618163998778
1106	50-51	0.1838234479991101
1106	52-53	0.26970793182557884
1106	54-55	0.2702626038719913
1106	56-57	0.30110988307101394
1106	58-59	0.25446866938541746
1106	60-61	0.16567960631351042
1106	62-63	0.24234994625526696
1106	64-65	0.29326109891243846
1106	66-67	0.26108677357956367
1106	68-69	0.282519100967896
1106	70-71	0.33392240749824964
1106	72-73	0.30073300266514025
1106	74-75	0.25786191786348667
1106	76-77	0.25092743153037134
1106	78-79	0.22895481302325038
1106	80-81	0.3134148678783717
1106	82-83	0.19911178313833489
1106	84-85	0.3213962074066039
1106	86-87	0.3181591486693556
1106	88-89	0.29646364123067315
1106	90-91	0.28291528251101283
1106	92-93	0.2490765936973247
1106	94-95	0.23824717001568274
1106	96-97	0.2526078278873207
1106	98-99	0.23493955344117623
1106	100	0.16160122779266217
1107	1	0.05625733640692232
1107	2	0.08502199918171272
1107	3	0.017725150590813143
1107	4	0.0014943487362302221
1107	5	0.04582730341706309
1107	6	0.05346959162609721
1107	7	0.08433252158128113
1107	8	0.04317552704826966
1107	9	0.12670223558730243
1107	10-11	0.04406498572255657
1107	12-13	0.02859989389460793
1107	14-15	0.13533673994160722
1107	16-17	0.0987481012115694
1107	18-19	0.16101798615144247
1107	20-21	0.09722527732900943
1107	22-23	0.06843726694844321
1107	24-25	0.060026839139283084
1107	26-27	0.10897389378995825
1107	28-29	0.16714453075149294
1107	30-31	0.24641246683464857
1107	32-33	0.2450634532713707
1107	34-35	0.2429979492803156
1107	36-37	0.2594731968225048
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2216	28-29	-0.3686544166474519
2216	30-31	-0.450299604730624
2216	32-33	-0.5571527842356403
2216	34-35	-0.6296901222076272
2216	36-37	-0.7005093564841474
2216	38-39	-0.6190846009592832
2216	40-41	-0.6074373135233202
2216	42-43	-0.6052584724050121
2216	44-45	-0.6278276589110803
2216	46-47	-0.7906201419335588
2216	48-49	-0.8381310934433444
2216	50-51	-0.784026261614784
2216	52-53	-0.793559005286518
2216	54-55	-0.736990817200315
2216	56-57	-0.8218351367450367
2216	58-59	-0.7949349797583807
2216	60-61	-0.9099980651139958
2216	62-63	-0.7776801517033078
2216	64-65	-0.9911046317887227
2216	66-67	-0.8929929642005376
2216	68-69	-0.8682927026662952
2216	70-71	-0.8587696461421714
2216	72-73	-0.7940336602626559
2216	74-75	-0.7607118875653569
2216	76-77	-0.765219842828408
2216	78-79	-0.8739298862464224
2216	80-81	-0.8359682174427974
2216	82-83	-0.7960183297757482
2216	84-85	-0.7905245782560506
2216	86-87	-0.7471149421604224
2216	88-89	-0.7350965297606251
2216	90-91	-0.7257623390647971
2216	92-93	-0.7979192187606117
2216	94-95	-0.7944898124614497
2216	96-97	-0.8595005247635434
2216	98-99	-0.8596509149154592
2216	100	-0.8165568768460396
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5.0
3	1.0
4	5.0
5	54.0
6	211.0
7	436.0
8	516.0
9	722.0
10	1185.0
11	2057.0
12	3310.0
13	4899.0
14	5277.0
15	7109.0
16	9385.0
17	13520.0
18	14970.0
19	7530.0
20	3761.0
21	3029.0
22	2795.0
23	3000.0
24	3405.0
25	4378.0
26	5226.0
27	6446.0
28	6268.0
29	7871.0
30	9439.0
31	12162.0
32	14941.0
33	19482.0
34	26265.0
35	41118.0
36	66775.0
37	160043.0
38	355917.0
39	227645.0
40	781.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.54941122899945	21.5620327072819	14.63709328600477	21.251462777713876
2	14.68497963746706	23.078583026279265	40.416585862964524	21.819851473289148
3	17.875024835137467	27.75071130685583	33.647072020472706	20.727191837533997
4	11.08468075841802	18.922516283664237	41.77944726336516	28.21335569455259
5	10.349918388183257	39.454203745302685	37.32107904415784	12.87479882235622
6	25.809325075221384	41.636189923899245	18.24031866298442	14.314166337894942
7	21.960575173538825	36.562552879194925	24.515317617398942	16.961554329867308
8	23.0918677293552	34.917371302269395	22.564426206257146	19.42633476211826
9	23.816852818515493	16.459822476825686	24.386858683986123	35.336466020672695
10-11	21.735528390904797	27.248015331687487	30.887579983249978	20.12887629415774
12-13	24.228091389416488	25.811502198798784	30.735081345098287	19.225325066686437
14-15	20.866591408710118	27.466318103499493	30.342011764006894	21.325078723783495
16-17	17.73819584595685	30.869660693253127	31.08925517544268	20.302888285347347
18-19	17.528020887152625	28.898742654632375	34.82570511489027	18.747531343324727
20-21	19.516911151692256	27.343315534455897	34.17560333821638	18.96416997563547
22-23	18.550605740273173	27.63547402123323	34.699687053204755	19.11423318528884
24-25	19.4352497400027	27.51926451659515	34.3096144815169	18.73587126188525
26-27	18.810861788185054	27.12880191582428	35.26768016230127	18.7926561336894
28-29	18.330081119173645	28.127889261513673	35.00398109041472	18.538048528897964
30-31	19.185468865726104	27.985050148176953	34.25573526098064	18.573745725116304
32-33	18.618739538264578	27.76963526010709	34.97714997725219	18.634475224376146
34-35	19.130295871323003	27.225619772235515	34.616959054596244	19.02712530184523
36-37	19.1934104955442	27.295965673562193	34.673591675534524	18.837032155359086
38-39	19.122378390510328	27.302541795014935	34.69487903482736	18.880200779647378
40-41	18.867712769903573	27.366382155500226	34.45016806259119	19.315737012005012
42-43	18.932296967955878	27.405449574691936	33.657839613864795	20.00441384348739
44-45	19.461492820808306	27.420529771389717	33.25224021137185	19.865737196430125
46-47	19.97967225314897	28.092974698331385	32.43612724065588	19.491225807863774
48-49	19.897527059974458	27.689629182709787	32.13113833656575	20.28170542075001
50-51	19.036187690066146	27.95280292695322	32.410373001911424	20.600636381069208
52-53	19.512135936188557	28.29867554332414	31.489723518003586	20.699465002483713
54-55	19.13156810328337	28.27383303244545	31.53474707812014	21.059851786151036
56-57	19.74242474701581	27.57915419784862	31.04314647154255	21.635274583593016
58-59	19.731669165835726	27.436784977583596	31.37992190413847	21.45162395244221
60-61	19.50416675190307	27.91193327107508	31.42944542737216	21.15445454964969
62-63	18.860758951699573	28.06761650993729	31.40010780050342	21.671516737859715
64-65	18.652079635796373	28.5092576660814	31.243921938439396	21.594740759682836
66-67	18.796916359654105	27.898874316321727	30.893583607786958	22.410625716237213
68-69	19.225895442507067	28.270107173616697	30.56843654283807	21.93556084103816
70-71	19.70918451981746	27.922449839035266	30.26769150477903	22.10067413636824
72-73	19.322888494484946	27.593472625313826	31.27210798344771	21.81153089675352
74-75	19.28424556937237	28.619149969722578	30.266108586144252	21.8304958747608
76-77	19.54496389285115	27.987947964638614	30.241359166909472	22.22572897560076
78-79	19.712597403461608	27.771905024911142	30.789618029182304	21.72587954244495
80-81	19.930071758201905	27.97362575937403	30.162067921464768	21.934234560959297
82-83	19.309055251909214	27.796402159255813	30.7693953009521	22.12514728788287
84-85	19.17801317376768	28.091505305916026	30.195857364352875	22.534624155963417
86-87	19.409317396361278	28.181433177224267	29.829918279210084	22.579331147204368
88-89	19.193954396551888	28.061919999087397	29.643809806281357	23.100315798079354
90-91	19.160709795695844	27.620583561840473	29.73720371904578	23.481502923417903
92-93	19.457387591088565	28.11390877430474	29.482013766131665	22.946689868475026
94-95	19.048531201889098	27.79230396656056	29.701316102807073	23.45784872874327
96-97	18.542983087390212	27.950010314305896	29.814380518985452	23.692626079318437
98-99	19.217654255617482	27.920392722391696	28.91037408062635	23.95157894136447
100	19.134379464968976	27.646279869840363	28.71050507681529	24.508835588375373
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	494.0
1	347.0
2	607.0
3	1049.0
4	615.0
5	239.5
6	581.0
7	1140.5
8	1740.0
9	1934.0
10	1279.5
11	634.5
12	592.0
13	637.0
14	705.5
15	774.0
16	885.5
17	1022.0
18	1164.5
19	1395.0
20	1675.5
21	2077.0
22	2854.5
23	4173.5
24	5666.0
25	7551.5
26	10358.0
27	13797.0
28	19022.0
29	23391.0
30	25802.0
31	29180.5
32	33157.5
33	36718.0
34	40115.0
35	42575.5
36	43489.5
37	43318.5
38	41582.0
39	40738.0
40	40096.5
41	37826.5
42	35373.0
43	33416.0
44	32018.0
45	30710.0
46	30480.5
47	34460.5
48	43112.5
49	42359.5
50	34466.5
51	28342.5
52	21316.0
53	17486.0
54	15883.5
55	15079.0
56	14070.5
57	12361.0
58	10555.0
59	8638.5
60	6964.5
61	5547.5
62	4205.5
63	3085.5
64	2329.0
65	1799.0
66	1379.5
67	1002.5
68	728.0
69	543.0
70	385.5
71	260.0
72	200.5
73	179.0
74	150.0
75	109.5
76	69.5
77	47.0
78	31.5
79	14.0
80	9.5
81	8.5
82	4.0
83	0.5
84	0.5
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.9012509280481093E-4
2	6.654378248168383E-4
3	1.9012509280481093E-4
4	0.011217380475483846
5	7.605003712192437E-4
6	0.004182752041705841
7	0.001235813103231271
8	0.13270731477775805
9	0.0019012509280481092
10-11	0.0
12-13	9.506254640240546E-5
14-15	1.425938196036082E-4
16-17	0.0
18-19	4.753127320120273E-5
20-21	0.0
22-23	2.851876392072164E-4
24-25	4.753127320120273E-4
26-27	0.0064642531553635715
28-29	0.0086982229958201
30-31	0.014829757238775252
32-33	0.018014352543255834
34-35	0.026855169358679543
36-37	0.036884268004133317
38-39	0.0416373953242536
40-41	0.05180908778931098
42-43	0.06663884502808623
44-45	0.10357064430542075
46-47	0.11012996000718672
48-49	0.10694536470270614
50-51	0.1096546472751747
52-53	0.10390336321782917
54-55	0.11027255382679033
56-57	0.09354154565996697
58-59	0.08774273032942025
60-61	0.08940632489146233
62-63	0.08779026160262145
64-65	0.0
66-67	4.753127320120273E-5
68-69	9.506254640240546E-5
70-71	4.753127320120273E-5
72-73	0.0
74-75	0.0
76-77	4.753127320120273E-5
78-79	0.0
80-81	3.3271891240841913E-4
82-83	2.3765636600601365E-4
84-85	0.0
86-87	3.3271891240841913E-4
88-89	9.506254640240546E-5
90-91	2.3765636600601365E-4
92-93	9.030941908228519E-4
94-95	7.12969098018041E-4
96-97	1.9012509280481093E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1051939.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.577066678729246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.99295852152852	40.01157456149979
2	16.358104840228684	18.18270966886066
3	5.828819574564153	9.718460824615024
4	2.533203005682276	5.6315196943024475
5	1.2478879752199368	3.467697660319143
6	0.6998575209470428	2.3337616864370365
7	0.38705651311869416	1.5058025946623874
8	0.2445155545299459	1.087156582247781
9	0.1390989118003114	0.6957638546458124
>10	0.47530281379318684	4.36056633269685
>50	0.0397539911608433	1.509629926044796
>100	0.04453398302287705	4.8493243149488325
>500	0.005309819740554524	2.062112034016477
>1k	0.003083121139676821	2.9026830459436073
>5k	5.138535232794702E-4	1.681237218759348
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6015	0.5718012166104689	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	5860	0.557066521918096	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5786	0.550031893484318	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	3501	0.3328139749548215	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3109	0.29554945676507854	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	2577	0.24497618207899888	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT	2527	0.2402230547588786	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	1962	0.1865127160415195	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	1502	0.142783944696413	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	1500	0.1425938196036082	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	1489	0.14154813159318172	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	1438	0.13669994172665906	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	1437	0.13660487918025666	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1363	0.12957025074647865	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1321	0.12557762379757761	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1310	0.12453193578715116	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	1200	0.11407505568288656	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	1181	0.11226886730124086	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.506254640240546E-5	0.0
10-11	0.0	0.0	0.0	1.425938196036082E-4	0.0
12-13	0.0	0.0	0.0	3.8025018560962186E-4	0.0
14-15	0.0	0.0	0.0	4.753127320120273E-4	0.0
16-17	0.0	0.0	0.0	5.703752784144328E-4	0.0
18-19	0.0	0.0	0.0	0.001093219283627663	0.0
20-21	0.0	0.0	0.0	0.0017111258352432983	0.0
22-23	0.0	0.0	0.0	0.004135220768504638	0.0
24-25	0.0	0.0	0.0	0.009981567372252573	0.0
26-27	0.0	0.0	0.0	0.017111258352432983	0.0
28-29	0.0	0.0	0.0	0.036884268004133317	0.0
30-31	0.0	0.0	0.0	0.0852235728497565	0.0
32-33	0.0	0.0	0.0	0.1668823002094228	0.0
34-35	0.0	0.0	0.0	0.2907963294449583	0.0
36-37	0.0	0.0	0.0	0.48073129715696444	0.0
38-39	0.0	0.0	0.0	0.7364020157062339	0.0
40-41	0.0	0.0	0.0	1.0183575283357684	0.0
42-43	0.0	0.0	0.0	1.2659479304408334	0.0
44-45	0.0	0.0	0.0	1.545051566678296	0.0
46-47	0.0	0.0	0.0	1.8872767337269556	0.0
48-49	0.0	0.0	0.0	2.2329716837193034	0.0
50-51	0.0	0.0	0.0	2.57785860206723	0.0
52-53	0.0	0.0	0.0	2.907725638083577	0.0
54-55	0.0	0.0	0.0	3.2805609450738116	0.0
56-57	0.0	0.0	0.0	3.725025880778258	0.0
58-59	0.0	0.0	0.0	4.182609447886237	0.0
60-61	0.0	0.0	0.0	4.642474516107873	0.0
62-63	0.0	0.0	0.0	5.095067299529726	0.0
64-65	0.0	0.0	0.0	5.5769868785167205	0.0
66-67	0.0	0.0	0.0	6.1350990884452425	0.0
68-69	0.0	0.0	0.0	6.769451460588494	0.0
70-71	0.0	0.0	0.0	7.430754064636828	0.0
72-73	0.0	0.0	0.0	8.080221381658061	0.0
74-75	0.0	0.0	0.0	8.750982709073433	0.0
76-77	0.0	0.0	0.0	9.450167737863126	0.0
78-79	0.0	0.0	0.0	10.277306954110458	0.0
80-81	0.0	0.0	0.0	11.131206277170065	0.0
82-83	0.0	0.0	0.0	11.972699937924158	0.0
84-85	0.0	0.0	0.0	12.826266542071355	0.0
86-87	0.0	0.0	0.0	13.692476464890074	0.0
88	0.0	0.0	0.0	14.392279400231383	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGCG	55	0.0027087573	34.20276	5
GCGGTAT	65	0.0061641303	28.913277	1
CATGGGG	1800	0.0	23.516634	4
GTACATG	4345	0.0	23.1406	1
TACATGG	4515	0.0	21.980108	2
GAGTACT	7920	0.0	21.381992	12-13
ACATGGG	4555	0.0	20.961037	3
TCGCGCG	115	0.003970564	20.423975	9
CTATACT	370	0.0	20.338709	4
AGTACTT	8175	0.0	19.479599	12-13
GTACTTT	9045	0.0	18.878345	14-15
TATTGCG	125	0.0063916985	18.809725	8
GTATTAG	375	9.094947E-12	18.793629	1
TATCGCG	130	0.008014546	18.086275	7
CTTACAC	350	1.4952093E-9	17.4695	3
TGTATCG	135	0.009955128	17.41807	5
AGAGTAC	11535	0.0	17.266977	10-11
GTACTAG	220	3.2034302E-5	17.08512	1
TAGAGAG	445	7.2759576E-12	16.909227	5
TCTATAC	365	2.6102498E-9	16.751574	3
>>END_MODULE