##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033003_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1474 Sequences flagged as poor quality 0 Sequence length 100 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 17.120759837177747 22.0 2.0 30.0 2.0 31.0 2 17.01967435549525 20.0 2.0 30.0 2.0 31.0 3 16.96268656716418 20.0 2.0 30.0 2.0 31.0 4 18.741519674355494 19.0 2.0 35.0 2.0 37.0 5 18.854138398914518 22.0 2.0 35.0 2.0 37.0 6 19.01967435549525 25.0 2.0 35.0 2.0 37.0 7 18.85820895522388 23.0 2.0 35.0 2.0 37.0 8 18.594301221166894 22.0 2.0 35.0 2.0 37.0 9 19.14111261872456 22.0 2.0 35.0 2.0 39.0 10-11 19.031886024423336 23.0 2.0 35.0 2.0 39.0 12-13 18.800542740841248 20.0 2.0 35.0 2.0 39.0 14-15 19.058344640434193 18.5 2.0 36.0 2.0 40.0 16-17 18.71302578018996 17.0 2.0 36.0 2.0 40.0 18-19 18.721506105834465 17.5 2.0 36.0 2.0 40.0 20-21 18.576662143826322 17.5 2.0 36.0 2.0 40.0 22-23 18.309362279511532 16.0 2.0 36.0 2.0 40.0 24-25 17.91248303934871 10.0 2.0 36.0 2.0 40.0 26-27 17.4966078697422 8.5 2.0 35.0 2.0 40.0 28-29 17.19335142469471 2.0 2.0 35.5 2.0 39.5 30-31 16.987109905020354 2.0 2.0 35.0 2.0 39.0 32-33 16.737109905020354 2.0 2.0 35.0 2.0 39.5 34-35 16.543419267299868 2.0 2.0 34.5 2.0 39.5 36-37 16.46506105834464 2.0 2.0 34.5 2.0 39.5 38-39 16.385345997286294 2.0 2.0 34.0 2.0 40.0 40-41 16.03731343283582 2.0 2.0 34.5 2.0 39.0 42-43 15.884667571234736 2.0 2.0 34.0 2.0 39.0 44-45 15.7516960651289 2.0 2.0 34.0 2.0 39.0 46-47 15.60719131614654 2.0 2.0 33.5 2.0 39.0 48-49 15.348710990502035 2.0 2.0 33.0 2.0 39.0 50-51 15.187245590230665 2.0 2.0 33.0 2.0 39.0 52-53 14.91350067842605 2.0 2.0 33.0 2.0 39.0 54-55 14.83819538670285 2.0 2.0 33.0 2.0 38.5 56-57 14.603459972862957 2.0 2.0 32.5 2.0 38.0 58-59 14.422659430122117 2.0 2.0 32.0 2.0 38.0 60-61 14.205563093622795 2.0 2.0 32.0 2.0 38.0 62-63 14.134328358208956 2.0 2.0 32.0 2.0 38.0 64-65 13.773744911804613 2.0 2.0 31.0 2.0 37.0 66-67 13.703188602442335 2.0 2.0 31.0 2.0 37.0 68-69 13.467096336499322 2.0 2.0 31.0 2.0 36.0 70-71 13.245590230664858 2.0 2.0 30.5 2.0 36.0 72-73 13.09158751696065 2.0 2.0 30.0 2.0 35.5 74-75 12.762211668928087 2.0 2.0 30.0 2.0 35.0 76-77 12.58955223880597 2.0 2.0 30.0 2.0 35.0 78-79 12.33649932157395 2.0 2.0 30.0 2.0 35.0 80-81 12.263568521031207 2.0 2.0 29.0 2.0 35.0 82-83 12.034938941655358 2.0 2.0 29.0 2.0 34.0 84-85 11.809362279511532 2.0 2.0 29.0 2.0 34.0 86-87 11.795454545454547 2.0 2.0 29.0 2.0 34.0 88-89 11.698778833107191 2.0 2.0 29.0 2.0 34.0 90-91 11.65739484396201 2.0 2.0 29.0 2.0 34.0 92-93 11.472523744911804 2.0 2.0 29.0 2.0 34.0 94-95 11.384328358208954 2.0 2.0 29.0 2.0 34.0 96-97 11.113636363636363 2.0 2.0 29.0 2.0 34.0 98-99 10.924694708276798 2.0 2.0 27.0 2.0 34.0 100 10.814789687924016 2.0 2.0 27.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -13.658730158730158 1101 2 -13.997354497354499 1101 3 -13.943121693121693 1101 4 -15.607142857142858 1101 5 -15.109788359788357 1101 6 -16.17063492063492 1101 7 -15.727513227513228 1101 8 -15.858465608465607 1101 9 -17.034391534391535 1101 10-11 -15.771825396825395 1101 12-13 -15.630291005291 1101 14-15 -16.365079365079367 1101 16-17 -15.456349206349206 1101 18-19 -15.6765873015873 1101 20-21 -15.371031746031743 1101 22-23 -15.017195767195766 1101 24-25 -14.988756613756614 1101 26-27 -15.035714285714285 1101 28-29 -14.217592592592592 1101 30-31 -14.055555555555554 1101 32-33 -14.5542328042328 1101 34-35 -13.917328042328041 1101 36-37 -14.087301587301585 1101 38-39 -14.082671957671955 1101 40-41 -14.25793650793651 1101 42-43 -14.137566137566136 1101 44-45 -14.037698412698415 1101 46-47 -13.880291005291005 1101 48-49 -13.411375661375663 1101 50-51 -13.45899470899471 1101 52-53 -13.162698412698413 1101 54-55 -12.909391534391533 1101 56-57 -12.655423280423278 1101 58-59 -12.060185185185185 1101 60-61 -11.430555555555555 1101 62-63 -11.73280423280423 1101 64-65 -11.751322751322746 1101 66-67 -12.06084656084656 1101 68-69 -11.952380952380954 1101 70-71 -11.383597883597886 1101 72-73 -11.019179894179894 1101 74-75 -10.496031746031745 1101 76-77 -10.523148148148145 1101 78-79 -10.320767195767194 1101 80-81 -9.939814814814813 1101 82-83 -9.960978835978835 1101 84-85 -9.431878306878309 1101 86-87 -9.135582010582011 1101 88-89 -9.529761904761907 1101 90-91 -9.753306878306878 1101 92-93 -9.176587301587304 1101 94-95 -9.501984126984127 1101 96-97 -9.648809523809526 1101 98-99 -9.497354497354497 1101 100 -9.226190476190474 1102 1 0.8412698412698418 1102 2 2.0026455026455015 1102 3 2.056878306878307 1102 4 1.8928571428571423 1102 5 1.3902116402116427 1102 6 0.3293650793650791 1102 7 1.772486772486772 1102 8 1.1415343915343925 1102 9 0.46560846560846514 1102 10-11 2.7281746031746046 1102 12-13 2.3697089947090006 1102 14-15 3.1349206349206327 1102 16-17 2.7936507936507944 1102 18-19 2.3234126984126995 1102 20-21 1.8789682539682566 1102 22-23 3.9828042328042343 1102 24-25 0.511243386243386 1102 26-27 3.9642857142857153 1102 28-29 3.5324074074074083 1102 30-31 4.194444444444446 1102 32-33 2.9457671957672 1102 34-35 4.582671957671959 1102 36-37 4.162698412698415 1102 38-39 5.1673280423280445 1102 40-41 4.49206349206349 1102 42-43 4.112433862433864 1102 44-45 2.212301587301585 1102 46-47 4.869708994708995 1102 48-49 3.838624338624337 1102 50-51 4.291005291005289 1102 52-53 4.837301587301587 1102 54-55 5.590608465608467 1102 56-57 5.344576719576722 1102 58-59 6.439814814814815 1102 60-61 7.069444444444445 1102 62-63 5.767195767195769 1102 64-65 4.748677248677254 1102 66-67 2.18915343915344 1102 68-69 1.7976190476190457 1102 70-71 5.616402116402114 1102 72-73 6.480820105820106 1102 74-75 5.503968253968255 1102 76-77 5.476851851851855 1102 78-79 5.4292328042328055 1102 80-81 5.560185185185187 1102 82-83 6.289021164021165 1102 84-85 6.318121693121691 1102 86-87 4.614417989417989 1102 88-89 2.220238095238093 1102 90-91 4.996693121693122 1102 92-93 6.823412698412696 1102 94-95 0.9980158730158735 1102 96-97 5.6011904761904745 1102 98-99 6.002645502645503 1102 100 6.7738095238095255 1103 1 -13.658730158730158 1103 2 -13.997354497354499 1103 3 -13.943121693121693 1103 4 -15.607142857142858 1103 5 -15.109788359788357 1103 6 -16.17063492063492 1103 7 -15.727513227513228 1103 8 -15.858465608465607 1103 9 -17.034391534391535 1103 10-11 -15.771825396825395 1103 12-13 -15.630291005291 1103 14-15 -16.365079365079367 1103 16-17 -15.456349206349206 1103 18-19 -15.6765873015873 1103 20-21 -15.371031746031743 1103 22-23 -15.017195767195766 1103 24-25 -14.988756613756614 1103 26-27 -15.035714285714285 1103 28-29 -14.217592592592592 1103 30-31 -14.055555555555554 1103 32-33 -14.5542328042328 1103 34-35 -13.917328042328041 1103 36-37 -14.087301587301585 1103 38-39 -14.082671957671955 1103 40-41 -14.25793650793651 1103 42-43 -14.137566137566136 1103 44-45 -14.037698412698415 1103 46-47 -13.880291005291005 1103 48-49 -13.411375661375663 1103 50-51 -13.45899470899471 1103 52-53 -13.162698412698413 1103 54-55 -12.909391534391533 1103 56-57 -12.655423280423278 1103 58-59 -12.060185185185185 1103 60-61 -11.430555555555555 1103 62-63 -11.73280423280423 1103 64-65 -11.751322751322746 1103 66-67 -12.06084656084656 1103 68-69 -11.952380952380954 1103 70-71 -11.383597883597886 1103 72-73 -11.019179894179894 1103 74-75 -10.496031746031745 1103 76-77 -10.523148148148145 1103 78-79 -10.320767195767194 1103 80-81 -9.939814814814813 1103 82-83 -9.960978835978835 1103 84-85 -9.431878306878309 1103 86-87 -9.135582010582011 1103 88-89 -9.529761904761907 1103 90-91 -9.753306878306878 1103 92-93 -9.176587301587304 1103 94-95 -9.501984126984127 1103 96-97 -9.648809523809526 1103 98-99 -9.497354497354497 1103 100 -9.226190476190474 1104 1 -9.658730158730158 1104 2 -9.997354497354499 1104 3 -6.943121693121693 1104 4 -8.607142857142858 1104 5 -0.10978835978835733 1104 6 -3.170634920634921 1104 7 0.7724867724867721 1104 8 -3.8584656084656075 1104 9 -0.5343915343915349 1104 10-11 -3.5218253968253954 1104 12-13 -1.3802910052909994 1104 14-15 -1.3650793650793673 1104 16-17 0.043650793650794384 1104 18-19 -3.4265873015873005 1104 20-21 -0.12103174603174338 1104 22-23 -2.5171957671957657 1104 24-25 3.011243386243386 1104 26-27 0.9642857142857153 1104 28-29 0.7824074074074083 1104 30-31 0.19444444444444642 1104 32-33 -8.8042328042328 1104 34-35 -0.167328042328041 1104 36-37 -1.8373015873015852 1104 38-39 -0.8326719576719555 1104 40-41 -5.75793650793651 1104 42-43 -3.637566137566136 1104 44-45 1.2123015873015852 1104 46-47 -2.3802910052910047 1104 48-49 0.5886243386243368 1104 50-51 3.5410052910052894 1104 52-53 0.337301587301587 1104 54-55 2.090608465608467 1104 56-57 -1.9054232804232782 1104 58-59 1.9398148148148149 1104 60-61 -2.1805555555555554 1104 62-63 -4.482804232804231 1104 64-65 -5.001322751322746 1104 66-67 -1.31084656084656 1104 68-69 2.5476190476190457 1104 70-71 -11.383597883597886 1104 72-73 -11.019179894179894 1104 74-75 -10.496031746031745 1104 76-77 -10.523148148148145 1104 78-79 -10.320767195767194 1104 80-81 -9.939814814814813 1104 82-83 -9.960978835978835 1104 84-85 -9.431878306878309 1104 86-87 -9.135582010582011 1104 88-89 -9.529761904761907 1104 90-91 -9.753306878306878 1104 92-93 -9.176587301587304 1104 94-95 -9.501984126984127 1104 96-97 -9.648809523809526 1104 98-99 -9.497354497354497 1104 100 -9.226190476190474 1105 1 -13.658730158730158 1105 2 -13.997354497354499 1105 3 -13.943121693121693 1105 4 -15.607142857142858 1105 5 -15.109788359788357 1105 6 -16.17063492063492 1105 7 -15.727513227513228 1105 8 -15.858465608465607 1105 9 -17.034391534391535 1105 10-11 -15.771825396825395 1105 12-13 -15.630291005291 1105 14-15 -16.365079365079367 1105 16-17 -15.456349206349206 1105 18-19 -15.6765873015873 1105 20-21 -15.371031746031743 1105 22-23 -15.017195767195766 1105 24-25 -14.988756613756614 1105 26-27 -15.035714285714285 1105 28-29 -14.217592592592592 1105 30-31 -14.055555555555554 1105 32-33 -14.5542328042328 1105 34-35 -13.917328042328041 1105 36-37 -14.087301587301585 1105 38-39 -14.082671957671955 1105 40-41 -14.25793650793651 1105 42-43 -14.137566137566136 1105 44-45 -14.037698412698415 1105 46-47 -13.880291005291005 1105 48-49 -13.411375661375663 1105 50-51 -13.45899470899471 1105 52-53 -13.162698412698413 1105 54-55 -12.909391534391533 1105 56-57 -12.655423280423278 1105 58-59 -12.060185185185185 1105 60-61 -11.430555555555555 1105 62-63 -11.73280423280423 1105 64-65 -11.751322751322746 1105 66-67 -12.06084656084656 1105 68-69 -11.952380952380954 1105 70-71 -11.383597883597886 1105 72-73 -11.019179894179894 1105 74-75 -10.496031746031745 1105 76-77 -10.523148148148145 1105 78-79 -10.320767195767194 1105 80-81 -9.939814814814813 1105 82-83 -9.960978835978835 1105 84-85 -9.431878306878309 1105 86-87 -9.135582010582011 1105 88-89 -9.529761904761907 1105 90-91 -9.753306878306878 1105 92-93 -9.176587301587304 1105 94-95 -9.501984126984127 1105 96-97 -9.648809523809526 1105 98-99 -9.497354497354497 1105 100 -9.226190476190474 1106 1 14.341269841269842 1106 2 15.335978835978834 1106 3 9.723544973544975 1106 4 15.059523809523807 1106 5 8.890211640211643 1106 6 10.496031746031747 1106 7 16.939153439153436 1106 8 17.808201058201057 1106 9 15.63227513227513 1106 10-11 15.894841269841276 1106 12-13 17.20304232804233 1106 14-15 17.301587301587304 1106 16-17 12.87698412698413 1106 18-19 14.99007936507937 1106 20-21 18.79563492063492 1106 22-23 17.316137566137563 1106 24-25 19.511243386243386 1106 26-27 20.130952380952387 1106 28-29 16.28240740740741 1106 30-31 20.944444444444446 1106 32-33 16.9457671957672 1106 34-35 8.749338624338623 1106 36-37 13.74603174603175 1106 38-39 8.750661375661377 1106 40-41 13.575396825396826 1106 42-43 14.362433862433864 1106 44-45 17.29563492063492 1106 46-47 19.286375661375665 1106 48-49 19.42195767195767 1106 50-51 18.04100529100529 1106 52-53 20.83730158730159 1106 54-55 12.590608465608467 1106 56-57 18.511243386243393 1106 58-59 18.273148148148145 1106 60-61 16.736111111111114 1106 62-63 14.26719576719577 1106 64-65 15.08201058201059 1106 66-67 17.93915343915344 1106 68-69 15.880952380952381 1106 70-71 9.94973544973545 1106 72-73 18.814153439153444 1106 74-75 20.003968253968253 1106 76-77 17.64351851851852 1106 78-79 12.34589947089947 1106 80-81 16.393518518518523 1106 82-83 16.205687830687832 1106 84-85 17.06812169312169 1106 86-87 19.197751322751323 1106 88-89 18.470238095238095 1106 90-91 19.413359788359784 1106 92-93 19.65674603174603 1106 94-95 17.83134920634921 1106 96-97 17.351190476190474 1106 98-99 17.835978835978835 1106 100 17.107142857142858 1107 1 -3.158730158730158 1107 2 -1.9973544973544985 1107 3 -6.943121693121693 1107 4 -4.107142857142858 1107 5 -0.10978835978835733 1107 6 0.3293650793650791 1107 7 -11.727513227513228 1107 8 -2.8584656084656075 1107 9 -1.0343915343915349 1107 10-11 1.2281746031746046 1107 12-13 2.3697089947090006 1107 14-15 2.6349206349206327 1107 16-17 3.0436507936507944 1107 18-19 -0.9265873015873005 1107 20-21 1.8789682539682566 1107 22-23 2.9828042328042343 1107 24-25 4.011243386243386 1107 26-27 -2.2857142857142847 1107 28-29 0.5324074074074083 1107 30-31 0.9444444444444464 1107 32-33 3.6957671957672 1107 34-35 2.332671957671959 1107 36-37 2.912698412698415 1107 38-39 2.4173280423280445 1107 40-41 3.2420634920634903 1107 42-43 3.112433862433864 1107 44-45 2.962301587301585 1107 46-47 3.8697089947089953 1107 48-49 1.8386243386243368 1107 50-51 1.7910052910052894 1107 52-53 0.08730158730158699 1107 54-55 1.090608465608467 1107 56-57 4.844576719576722 1107 58-59 5.689814814814815 1107 60-61 6.569444444444445 1107 62-63 3.517195767195769 1107 64-65 4.498677248677254 1107 66-67 0.18915343915343996 1107 68-69 1.2976190476190457 1107 70-71 3.6164021164021136 1107 72-73 -4.769179894179894 1107 74-75 -2.996031746031745 1107 76-77 -4.273148148148145 1107 78-79 -4.0707671957671945 1107 80-81 0.5601851851851869 1107 82-83 -0.7109788359788354 1107 84-85 -0.43187830687830875 1107 86-87 -1.3855820105820111 1107 88-89 4.970238095238093 1107 90-91 5.996693121693122 1107 92-93 -0.42658730158730407 1107 94-95 2.2480158730158735 1107 96-97 1.1011904761904745 1107 98-99 5.252645502645503 1107 100 4.2738095238095255 1108 1 -9.658730158730158 1108 2 -2.4973544973544985 1108 3 -0.943121693121693 1108 4 -0.6071428571428577 1108 5 -0.10978835978835733 1108 6 -1.170634920634921 1108 7 -0.7275132275132279 1108 8 -0.8584656084656075 1108 9 -2.034391534391535 1108 10-11 -0.7718253968253954 1108 12-13 -2.8802910052909994 1108 14-15 -4.865079365079367 1108 16-17 -2.9563492063492056 1108 18-19 -2.9265873015873005 1108 20-21 -6.871031746031743 1108 22-23 -5.517195767195766 1108 24-25 -11.238756613756614 1108 26-27 -7.535714285714285 1108 28-29 -1.4675925925925917 1108 30-31 -6.055555555555554 1108 32-33 -11.0542328042328 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-9.529761904761907 2216 90-91 -9.753306878306878 2216 92-93 -9.176587301587304 2216 94-95 -9.501984126984127 2216 96-97 -9.648809523809526 2216 98-99 -9.497354497354497 2216 100 -9.226190476190474 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 576.0 3 42.0 4 30.0 5 29.0 6 27.0 7 27.0 8 20.0 9 25.0 10 18.0 11 15.0 12 20.0 13 20.0 14 12.0 15 8.0 16 19.0 17 12.0 18 15.0 19 12.0 20 8.0 21 8.0 22 10.0 23 8.0 24 17.0 25 12.0 26 20.0 27 27.0 28 22.0 29 30.0 30 29.0 31 37.0 32 30.0 33 39.0 34 53.0 35 48.0 36 50.0 37 56.0 38 40.0 39 3.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.46164290563476 19.687712152070606 13.917175831636117 25.933469110658518 2 17.515274949083505 22.267481330617787 34.147997284453496 26.069246435845212 3 22.87847929395791 24.779361846571625 25.865580448065174 26.476578411405292 4 16.08961303462322 20.298710115410724 32.043448744059745 31.568228105906314 5 18.329938900203665 35.234215885947044 28.852681602172435 17.58316361167685 6 30.074677528852682 35.234215885947044 17.58316361167685 17.10794297352342 7 29.26001357773252 29.803122878479293 20.8418194161575 20.095044127630686 8 27.562797012898848 32.9938900203666 18.601493550577054 20.8418194161575 9 27.766463000678886 18.87304820095044 18.397827562797012 34.96266123557366 10-11 24.03258655804481 26.680244399185337 24.745417515274948 24.54175152749491 12-13 25.661914460285136 22.912423625254583 26.782077393075355 24.643584521384927 14-15 23.35369993211134 25.45824847250509 23.964697895451458 27.22335369993211 16-17 27.29124236252546 26.54446707399864 22.844534962661236 23.319755600814666 18-19 26.103190767141886 26.951799049558723 25.016972165648337 21.92803801765105 20-21 24.881194840461642 25.186693822131705 24.779361846571625 25.15274949083503 22-23 25.42430414120842 26.069246435845212 24.338085539714868 24.1683638832315 24-25 25.45824847250509 24.745417515274948 24.202308214528173 25.59402579769178 26-27 26.272912423625254 23.99864222674813 23.828920570264767 25.899524779361847 28-29 26.849966055668705 24.37202987101154 23.455532926001357 25.3224711473184 30-31 25.76374745417515 25.72980312287848 22.471147318397826 26.035302104548542 32-33 25.3224711473184 24.100475220638153 23.251866938221315 27.325186693822133 34-35 28.47929395790903 22.471147318397826 23.421588594704684 25.62797012898846 36-37 28.17379497623897 23.08214528173795 22.844534962661236 25.899524779361847 38-39 23.99864222674813 24.6775288526816 24.405974202308215 26.917854718262053 40-41 24.03258655804481 23.896809232858114 23.557365919891378 28.5132382892057 42-43 24.507807196198232 25.492192803801768 24.37202987101154 25.62797012898846 44-45 24.813306177868295 23.99864222674813 24.304141208418194 26.883910386965375 46-47 24.304141208418194 24.6775288526816 24.745417515274948 26.272912423625254 48-49 25.661914460285136 23.896809232858114 24.43991853360489 26.001357773251865 50-51 27.155465037338764 23.6591989137814 23.86286490156144 25.3224711473184 52-53 25.831636116768497 24.643584521384927 23.761031907671416 25.76374745417515 54-55 25.76374745417515 26.20502376103191 22.674813306177867 25.35641547861507 56-57 26.37474541751528 25.186693822131705 22.844534962661236 25.59402579769178 58-59 26.849966055668705 24.100475220638153 24.100475220638153 24.94908350305499 60-61 28.95451459606246 23.116089613034625 23.421588594704684 24.507807196198232 62-63 27.155465037338764 26.476578411405292 21.860149355057708 24.507807196198232 64-65 26.035302104548542 24.236252545824847 22.674813306177867 27.053632043448744 66-67 26.748133061778685 24.575695858791583 21.92803801765105 26.748133061778685 68-69 28.275627970128987 23.964697895451458 21.52070604209097 26.23896809232858 70-71 26.37474541751528 25.016972165648337 22.471147318397826 26.137135098438563 72-73 28.5132382892057 24.54175152749491 22.640868974881194 24.304141208418194 74-75 28.445349626612355 23.930753564154784 22.539035980991173 25.084860828241684 76-77 29.19212491513917 23.35369993211134 24.507807196198232 22.946367956551256 78-79 29.531568228105908 25.15274949083503 22.471147318397826 22.844534962661236 80-81 27.155465037338764 24.643584521384927 22.640868974881194 25.56008146639511 82-83 27.528852681602174 23.319755600814666 22.98031228784793 26.171079429735233 84-85 27.189409368635438 23.048200950441274 24.066530889341482 25.695858791581806 86-87 26.001357773251865 24.47386286490156 21.89409368635438 27.630685675492195 88-89 29.565512559402578 23.150033944331298 22.674813306177867 24.609640190088257 90-91 26.81602172437203 23.727087576374746 24.84725050916497 24.609640190088257 92-93 26.578411405295316 24.779361846571625 24.745417515274948 23.896809232858114 94-95 26.40868974881195 22.57298031228785 24.134419551934826 26.883910386965375 96-97 27.22335369993211 24.813306177868295 22.946367956551256 25.016972165648337 98-99 28.547182620502376 23.761031907671416 22.77664630006789 24.915139171758316 100 26.069246435845212 26.137135098438563 22.87847929395791 24.915139171758316 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.5 9 0.5 10 0.5 11 1.0 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 1.5 18 1.5 19 0.0 20 1.5 21 2.5 22 1.5 23 3.0 24 3.5 25 3.5 26 5.0 27 5.0 28 5.0 29 9.0 30 11.5 31 9.5 32 11.5 33 12.5 34 12.0 35 16.0 36 21.5 37 26.0 38 29.5 39 29.0 40 34.0 41 43.0 42 44.5 43 39.5 44 31.5 45 37.0 46 45.0 47 38.5 48 37.0 49 47.0 50 53.0 51 48.5 52 48.5 53 50.5 54 48.0 55 48.5 56 42.0 57 35.0 58 40.5 59 42.0 60 36.0 61 32.0 62 29.5 63 26.5 64 23.5 65 25.5 66 26.5 67 19.0 68 12.5 69 12.5 70 14.0 71 13.0 72 11.5 73 13.5 74 10.0 75 9.5 76 11.0 77 7.0 78 7.0 79 6.5 80 4.0 81 5.0 82 7.5 83 6.0 84 5.5 85 6.0 86 3.5 87 2.5 88 2.5 89 1.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06784260515603799 2 0.06784260515603799 3 0.06784260515603799 4 0.06784260515603799 5 0.06784260515603799 6 0.06784260515603799 7 0.06784260515603799 8 0.06784260515603799 9 0.06784260515603799 10-11 0.06784260515603799 12-13 0.06784260515603799 14-15 0.06784260515603799 16-17 0.06784260515603799 18-19 0.06784260515603799 20-21 0.06784260515603799 22-23 0.06784260515603799 24-25 0.06784260515603799 26-27 0.06784260515603799 28-29 0.06784260515603799 30-31 0.06784260515603799 32-33 0.06784260515603799 34-35 0.06784260515603799 36-37 0.06784260515603799 38-39 0.06784260515603799 40-41 0.06784260515603799 42-43 0.06784260515603799 44-45 0.06784260515603799 46-47 0.06784260515603799 48-49 0.06784260515603799 50-51 0.06784260515603799 52-53 0.06784260515603799 54-55 0.06784260515603799 56-57 0.06784260515603799 58-59 0.06784260515603799 60-61 0.06784260515603799 62-63 0.06784260515603799 64-65 0.06784260515603799 66-67 0.06784260515603799 68-69 0.06784260515603799 70-71 0.06784260515603799 72-73 0.06784260515603799 74-75 0.06784260515603799 76-77 0.06784260515603799 78-79 0.06784260515603799 80-81 0.06784260515603799 82-83 0.06784260515603799 84-85 0.06784260515603799 86-87 0.06784260515603799 88-89 0.06784260515603799 90-91 0.06784260515603799 92-93 0.06784260515603799 94-95 0.06784260515603799 96-97 0.06784260515603799 98-99 0.06784260515603799 100 0.06784260515603799 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1474.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.93215739484395 #Duplication Level Percentage of deduplicated Percentage of total 1 99.93211133740665 99.86431478968792 2 0.0678886625933469 0.13568521031207598 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT 2 0.13568521031207598 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.06784260515603799 0.0 30-31 0.0 0.0 0.0 0.13568521031207598 0.0 32-33 0.0 0.0 0.0 0.16960651289009498 0.0 34-35 0.0 0.0 0.0 0.20352781546811397 0.0 36-37 0.0 0.0 0.0 0.23744911804613295 0.0 38-39 0.0 0.0 0.0 0.33921302578018997 0.0 40-41 0.0 0.0 0.0 0.508819538670285 0.0 42-43 0.0 0.0 0.0 0.5766621438263229 0.0 44-45 0.0 0.0 0.0 0.8480325644504749 0.0 46-47 0.0 0.0 0.0 0.8819538670284939 0.0 48-49 0.0 0.0 0.0 0.9497964721845319 0.0 50-51 0.0 0.0 0.0 0.9497964721845319 0.0 52-53 0.0 0.0 0.0 0.983717774762551 0.0 54-55 0.0 0.0 0.0 1.1194029850746268 0.0 56-57 0.0 0.0 0.0 1.322930800542741 0.0 58-59 0.0 0.0 0.0 1.4586160108548167 0.0 60-61 0.0 0.0 0.0 1.662143826322931 0.0 62-63 0.0 0.0 0.0 1.7639077340569878 0.0 64-65 0.0 0.0 0.0 1.7978290366350067 0.0 66-67 0.0 0.0 0.0 1.967435549525102 0.0 68-69 0.0 0.0 0.0 2.1709633649932156 0.0 70-71 0.0 0.0 0.0 2.2048846675712346 0.0 72-73 0.0 0.0 0.0 2.2727272727272725 0.0 74-75 0.0 0.0 0.0 2.340569877883311 0.0 76-77 0.0 0.0 0.0 2.5101763907734056 0.0 78-79 0.0 0.0 0.0 2.611940298507463 0.0 80-81 0.0 0.0 0.0 2.7476255088195387 0.0 82-83 0.0 0.0 0.0 3.0529172320217097 0.0 84-85 0.0 0.0 0.0 3.3921302578018997 0.0 86-87 0.0 0.0 0.0 3.6295793758480324 0.0 88 0.0 0.0 0.0 3.7991858887381276 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE