FastQCFastQC Report
Wed 25 May 2016
SRR1033003_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033003_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCC110.7462686567164178No Hit
TGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACT100.6784260515603799No Hit
CCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCT80.5427408412483039No Hit
GTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTT60.40705563093622793No Hit
CTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGG60.40705563093622793No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG60.40705563093622793TruSeq Adapter, Index 1 (96% over 28bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG60.40705563093622793RNA PCR Primer, Index 33 (96% over 29bp)
CAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCA60.40705563093622793No Hit
GTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCG50.33921302578018997No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG50.33921302578018997RNA PCR Primer, Index 39 (96% over 30bp)
CCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTC50.33921302578018997No Hit
GTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTA50.33921302578018997No Hit
GTACATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGA50.33921302578018997No Hit
CGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGC50.33921302578018997No Hit
CTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTG50.33921302578018997No Hit
GTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGG40.27137042062415195No Hit
ACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGG40.27137042062415195No Hit
CTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGACCTGACCA40.27137042062415195No Hit
GTCTCGAAGAGGACGGGGGTAGGAAATCCTCCAGGATCTTAGGAAATTTC30.20352781546811397No Hit
GTTCTTCTCCTGCGTCTCCGTTTTCTTCAGCTTGGCCTTATCGAAGCTGG30.20352781546811397No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA30.20352781546811397RNA PCR Primer, Index 33 (96% over 29bp)
CCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAA30.20352781546811397No Hit
TTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTAC30.20352781546811397No Hit
GTGCAGCTTGTGGCTCGTGTCCATCTTGCAGGTGGCTCTTCCTCCACATC30.20352781546811397No Hit
GTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATG30.20352781546811397No Hit
GTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCC30.20352781546811397No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT30.20352781546811397TruSeq Adapter, Index 1 (96% over 26bp)
GTTTAATGAAGTCTGAAAGGTAAGCGCCATCGTGGTCAGAGATACGGAGG20.13568521031207598No Hit
GCTGTGGGAGCATCCACAGTCGAGCGGGGGTTCTTGATGGTTTCATCTAC20.13568521031207598No Hit
ATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAA20.13568521031207598No Hit
GTACTTGGCGTGCTGGCTGATTTCAATTTTCTTCACCATTTTCCGGAGGG20.13568521031207598No Hit
ATTTATTATAGGCCAGTGATGTCTCAAAGAGTAGAGGAGCGTCTACTGGT20.13568521031207598No Hit
GTCTACAAGGCTCTGAGTGACCACCACATCTACCTGGAAGGCACCTTGCT20.13568521031207598No Hit
GTACATGGGGACTTCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCC20.13568521031207598No Hit
GATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGA20.13568521031207598No Hit
GCCTTGAGGCCACCATTGAGAAGCTCCTGAGCAGTGAGAGCAAGCTGAAG20.13568521031207598No Hit
GAGCACGCCATGAAGGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGG20.13568521031207598No Hit
GGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTG20.13568521031207598No Hit
TTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGA20.13568521031207598No Hit
GGATCTGGCTGCGCTATGACTCCCGGAGCGGCACCCACAACATGTACCGG20.13568521031207598No Hit
GTGCCCAGGTTCACAGTGCAGCTTGTGGCTCGTGTCCATCTTGCAGGGGG20.13568521031207598No Hit
GCTATATACTCGTCGGTGGGGCCGGCGGTCAGTCTGCGGCAGCGGCAGCA20.13568521031207598No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA20.13568521031207598RNA PCR Primer, Index 39 (96% over 30bp)
GGTATCAACGCAGAGTACATGGGGCCTTTTGCGGGTGGCGGCGAACGCGG20.13568521031207598No Hit
CCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAA20.13568521031207598No Hit
CTATGACTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGACC20.13568521031207598No Hit
GCGCTATGACTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGG20.13568521031207598No Hit
ACCCACAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGCTGT20.13568521031207598No Hit
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT20.13568521031207598RNA PCR Primer, Index 31 (96% over 27bp)
GACCACCGCAGGCGCTGTCACCCAGTGCTACCGAGACATGGGTGCCCGGC20.13568521031207598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA20.13568521031207598No Hit
GGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCA20.13568521031207598No Hit
GGTTTTGCCACAGAAAGAGCAAGTGTACTTGGCGTGCTGGCTGATTTCAA20.13568521031207598No Hit
GTACTGGACTGTGTGCCAGAGGAGAGGCGTAAACTGATTGTGGCATATGT20.13568521031207598No Hit
CTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGG20.13568521031207598No Hit
CCCACAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGCTGTC20.13568521031207598No Hit
CTCCAGCCCCTTGCTGGTTATCTTACTCTGAGTGTGATGCAGTCAGAGGC20.13568521031207598No Hit
TTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTA20.13568521031207598No Hit
CGCGGAGAGCACGCCATGAAGGCCTCGGGCACGCTACGAGAGTACAAGGT20.13568521031207598No Hit
GCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTC20.13568521031207598No Hit
CCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGC20.13568521031207598No Hit
ACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGCTGTCACCCAG20.13568521031207598No Hit
CTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCG20.13568521031207598No Hit
AGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCC20.13568521031207598No Hit
GTCTGGAGATGAGATGACATCTCTGTCAGAGTATGTTTCTCGCATGAAGG20.13568521031207598No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)