FastQCFastQC Report
Wed 25 May 2016
SRR1033002_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033002_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3832174
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51100.037.619311
GGTATCA31650.037.571211
TCAACGC55050.033.6397634
ATCAACG56800.032.6033253
CAACGCA57200.032.292745
AACGCAG60750.030.4056746
GTACATG122900.028.3001141
TATCAAC68050.027.6274132
TACATGG122100.027.0227662
ACATGGG127050.025.2303773
ACGCAGA72300.025.0932757
CGCAGAG75500.023.9674688
CATGGGG81650.020.262854
GCAGAGT89000.019.4334169
TAGGTCG1508.2938926E-418.8004555
GAGTACA89700.017.815371
CATGGGA40750.017.0705744
AGAGTAC76900.016.47092810-11
CTACACT17600.016.2903794
ACCGCGT2607.3307074E-616.2696238
Produced by FastQC (version 0.11.5)