FastQCFastQC Report
Wed 25 May 2016
SRR1033002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033002_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3832174
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69690.18185499927717272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55470.1447481246937117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57100.049.119591
GTATCAA105850.043.0967861
TCAACGC126200.035.5585674
ATCAACG128300.034.976553
CAACGCA129000.034.7111055
AACGCAG131900.033.911836
TATCAAC137500.032.806492
ACGCAGA153050.029.0092957
CGCAGAG157950.028.1089888
GTACATG123250.026.4157431
TACATGG123100.025.4483762
GCAGAGT174750.024.972299
ACATGGG125000.023.9709193
GTGGTAT28000.022.4834651
TATACCG1703.4330715E-622.1341195
TACACCG4950.021.854655
ATACCGC2458.438292E-921.1174836
AGAGTAC160850.020.96775410-11
GAGTACT116250.020.64819712-13
CAGAGTA171850.020.336310-11
Produced by FastQC (version 0.11.5)