FastQCFastQC Report
Wed 25 May 2016
SRR1033001_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033001_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3046559
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42050.034.874461
TCAACGC39400.034.5955854
GGTATCA25400.034.0488931
ATCAACG41400.032.9242973
CAACGCA42250.032.2597965
AACGCAG43650.031.3317626
GTACATG91250.028.5361771
TATCAAC50050.027.797552
TACATGG92400.026.6041012
ACATGGG93300.025.4406743
ACGCAGA53350.025.3707987
CGCAGAG54900.024.3976848
CATGGGG64850.020.5838814
GCAGAGT67550.019.063449
GACGTAT1250.006411749418.800911
GAGTACA65550.018.7148651
CATGGGA33250.016.9632264
ACACCGT6550.016.5029896
GTATTAG14950.016.3486181
AGTACAT68200.016.3336352
Produced by FastQC (version 0.11.5)