##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033001_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3046559
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.711967829935347	33.0	31.0	34.0	30.0	34.0
2	31.721500880173338	34.0	31.0	34.0	30.0	34.0
3	31.881974384871587	34.0	31.0	34.0	30.0	34.0
4	35.18609815204629	37.0	35.0	37.0	33.0	37.0
5	35.27609542437878	37.0	35.0	37.0	33.0	37.0
6	35.33350347063687	37.0	37.0	37.0	33.0	37.0
7	35.27688122895371	37.0	37.0	37.0	33.0	37.0
8	35.291571573043555	37.0	37.0	37.0	33.0	37.0
9	36.94221250926045	39.0	38.0	39.0	34.0	39.0
10-11	36.93238010489867	39.0	38.0	39.0	34.0	39.0
12-13	36.87569352833803	39.0	38.0	39.0	34.0	39.0
14-15	38.36385705971885	41.0	39.0	41.0	34.0	41.0
16-17	38.38789040356678	41.0	39.0	41.0	34.0	41.0
18-19	38.39647123197023	41.0	39.0	41.0	34.0	41.0
20-21	38.44192037639842	41.0	39.0	41.0	34.0	41.0
22-23	38.434571265483456	41.0	39.0	41.0	34.0	41.0
24-25	38.46635909562231	41.0	39.0	41.0	34.0	41.0
26-27	38.44669034802871	41.0	39.0	41.0	34.0	41.0
28-29	38.42650921908947	41.0	39.0	41.0	34.0	41.0
30-31	38.3810823949249	41.0	39.0	41.0	34.0	41.0
32-33	38.332089580408585	41.0	39.0	41.0	34.0	41.0
34-35	38.25953690704824	41.0	39.0	41.0	33.5	41.0
36-37	38.169488921763865	41.0	39.0	41.0	33.0	41.0
38-39	38.07823449340715	41.0	38.0	41.0	33.0	41.0
40-41	37.993265188693215	41.0	38.0	41.0	33.0	41.0
42-43	37.877681672995664	40.5	38.0	41.0	33.0	41.0
44-45	37.74631707444366	40.0	38.0	41.0	33.0	41.0
46-47	37.625023674250194	40.0	38.0	41.0	32.5	41.0
48-49	37.49593114723857	40.0	38.0	41.0	32.0	41.0
50-51	37.361571366252875	40.0	37.0	41.0	32.0	41.0
52-53	37.20773255991432	40.0	37.0	41.0	31.5	41.0
54-55	37.045041471378035	40.0	37.0	41.0	31.0	41.0
56-57	36.864549972608444	40.0	36.0	41.0	31.0	41.0
58-59	36.66063450601153	40.0	36.0	41.0	31.0	41.0
60-61	36.41936427293875	39.0	35.0	41.0	31.0	41.0
62-63	36.1697708792116	39.0	35.0	41.0	30.0	41.0
64-65	35.89114128431453	38.5	35.0	40.5	30.0	41.0
66-67	35.59626302986419	38.0	35.0	40.0	30.0	41.0
68-69	35.28345717250183	37.0	35.0	40.0	29.5	41.0
70-71	34.945693649786534	37.0	34.5	39.5	29.0	41.0
72-73	34.65438401160128	36.0	34.0	39.0	29.0	41.0
74-75	34.37628452296509	36.0	34.0	39.0	29.0	41.0
76-77	34.05179285876295	35.5	34.0	38.5	29.0	40.0
78-79	33.68693384897519	35.0	34.0	37.0	28.5	39.0
80-81	33.36772158359645	35.0	34.0	37.0	28.0	39.0
82-83	33.05493131759471	35.0	34.0	36.5	28.0	39.0
84-85	32.896790936922606	35.0	34.0	36.0	28.0	37.5
86-87	32.668967513841025	35.0	34.0	36.0	28.0	37.0
88-89	32.439365690931965	35.0	34.0	35.5	27.0	37.0
90-91	32.25081805407346	35.0	34.0	35.0	27.0	36.0
92-93	32.07314284738946	35.0	34.0	35.0	27.0	36.0
94-95	31.90356661400616	35.0	33.0	35.0	26.5	36.0
96-97	31.744031873336443	35.0	33.0	35.0	26.0	36.0
98-99	31.576386835114633	35.0	33.0	35.0	25.0	35.5
100	31.456634517828146	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3106824012648737
1101	2	0.32795364392529436
1101	3	0.38323992461067746
1101	4	0.33966965888065204
1101	5	0.33418126403283566
1101	6	0.3232772701549038
1101	7	0.33486631729491734
1101	8	0.31154015814580305
1101	9	0.4189496458542692
1101	10-11	0.433545043577638
1101	12-13	0.2929455171671833
1101	14-15	0.25482035150261595
1101	16-17	0.2930560352642644
1101	18-19	0.34667866224961585
1101	20-21	0.3460353212589098
1101	22-23	0.3299131086103344
1101	24-25	0.33123115263674663
1101	26-27	0.38641177282585915
1101	28-29	0.34013141050290585
1101	30-31	0.3206565224638922
1101	32-33	0.32322172379384284
1101	34-35	0.3569678084290828
1101	36-37	0.31129043971188963
1101	38-39	0.31837844960399053
1101	40-41	0.32456393215434787
1101	42-43	0.3317547967860932
1101	44-45	0.2965518044214619
1101	46-47	0.36485857563815216
1101	48-49	0.35376450804356807
1101	50-51	0.3668235629399561
1101	52-53	0.3657931050723846
1101	54-55	0.30861765854395884
1101	56-57	0.35115259938390864
1101	58-59	0.31313350505497795
1101	60-61	0.3909349588812887
1101	62-63	0.4069170540073017
1101	64-65	0.3641135468152612
1101	66-67	0.40629336831345597
1101	68-69	0.33221933341363297
1101	70-71	0.38674446514055916
1101	72-73	0.3641789682888543
1101	74-75	0.35743212083689
1101	76-77	0.30953632103364725
1101	78-79	0.28555218196534327
1101	80-81	0.32540495735945996
1101	82-83	0.26333906789267303
1101	84-85	0.26599019677288993
1101	86-87	0.25021512744567787
1101	88-89	0.24072526202049715
1101	90-91	0.32753921202951375
1101	92-93	0.3813471042005574
1101	94-95	0.3800429698409715
1101	96-97	0.36865343933144956
1101	98-99	0.3338819520538756
1101	100	0.3398429589274379
1102	1	0.47168830869086875
1102	2	0.3785722656927284
1102	3	0.4575455064542524
1102	4	0.43718569864469714
1102	5	0.44972608515904255
1102	6	0.42260875785340346
1102	7	0.41357505712176845
1102	8	0.4927970912047641
1102	9	0.5406888054144332
1102	10-11	0.5262212370433232
1102	12-13	0.5333117210811622
1102	14-15	0.5764517545872252
1102	16-17	0.5796817193750599
1102	18-19	0.5632514830595952
1102	20-21	0.6038235246454491
1102	22-23	0.6098936415381857
1102	24-25	0.6401909793972465
1102	26-27	0.5959305922279441
1102	28-29	0.6104359478335368
1102	30-31	0.6590044300854174
1102	32-33	0.6408111057079893
1102	34-35	0.6941251242254438
1102	36-37	0.6642869968258296
1102	38-39	0.7265912107235337
1102	40-41	0.682139586509031
1102	42-43	0.708590586117289
1102	44-45	0.6594255434207525
1102	46-47	0.6258362468356182
1102	48-49	0.6538144712536891
1102	50-51	0.651769071379448
1102	52-53	0.6960130171368917
1102	54-55	0.6334881703470927
1102	56-57	0.6532919992679922
1102	58-59	0.5778853071526271
1102	60-61	0.6816218443222652
1102	62-63	0.6645067253617469
1102	64-65	0.6790950905722823
1102	66-67	0.6970976729859046
1102	68-69	0.645003595545937
1102	70-71	0.6987180100442174
1102	72-73	0.639633457498455
1102	74-75	0.6150157885169563
1102	76-77	0.5348037578685165
1102	78-79	0.4832284376796565
1102	80-81	0.44581066924261137
1102	82-83	0.49361363235295386
1102	84-85	0.4831945093322645
1102	86-87	0.493848562552877
1102	88-89	0.4490539003751408
1102	90-91	0.45138161671470556
1102	92-93	0.4889032150152417
1102	94-95	0.555139595820215
1102	96-97	0.5226146668439746
1102	98-99	0.5317645129786861
1102	100	0.5318642273546885
1103	1	0.1704693490741036
1103	2	0.2899805368714077
1103	3	0.3452811022507447
1103	4	0.3616014874977225
1103	5	0.23621596718174231
1103	6	0.20102180973788109
1103	7	0.2355540541943526
1103	8	0.29337373775096864
1103	9	0.3707499347019123
1103	10-11	0.3738322401322378
1103	12-13	0.36294895847981934
1103	14-15	0.4583673461359581
1103	16-17	0.4577199692875311
1103	18-19	0.3880068790652942
1103	20-21	0.42389212864217996
1103	22-23	0.37396923818177896
1103	24-25	0.363875234027347
1103	26-27	0.4061882822959646
1103	28-29	0.39903882929890244
1103	30-31	0.3958490179906491
1103	32-33	0.42633643637930874
1103	34-35	0.4693775899196382
1103	36-37	0.4720657832170403
1103	38-39	0.5011221064423594
1103	40-41	0.5294253041466348
1103	42-43	0.49273423826692664
1103	44-45	0.4284835990273521
1103	46-47	0.4637751650839945
1103	48-49	0.4102035193572391
1103	50-51	0.4006890475018139
1103	52-53	0.38249098472292076
1103	54-55	0.45062177018595406
1103	56-57	0.47372510580042615
1103	58-59	0.4893603113653455
1103	60-61	0.44010856460084824
1103	62-63	0.45680811002649335
1103	64-65	0.43988775712986694
1103	66-67	0.42261653756141015
1103	68-69	0.522433112206798
1103	70-71	0.48194208707913333
1103	72-73	0.4710331478449845
1103	74-75	0.4039695821375915
1103	76-77	0.40904121106778746
1103	78-79	0.4531870540570395
1103	80-81	0.4115907616511052
1103	82-83	0.443111230116088
1103	84-85	0.37927511674304526
1103	86-87	0.3255253016416475
1103	88-89	0.32356319165508296
1103	90-91	0.3636746643209463
1103	92-93	0.41411916037094443
1103	94-95	0.30555085718729913
1103	96-97	0.35886313678348714
1103	98-99	0.3500819080866968
1103	100	0.38666484641785104
1104	1	0.0399920591181413
1104	2	0.11670021003657638
1104	3	0.11508012892180375
1104	4	0.10372168957052708
1104	5	0.050073463029761456
1104	6	0.06052912706510227
1104	7	0.02971263389795098
1104	8	0.12635205485250367
1104	9	0.1591489764184928
1104	10-11	0.17643930920850437
1104	12-13	0.11240514999762041
1104	14-15	0.19227776168144572
1104	16-17	0.12428855078502465
1104	18-19	0.13763623864726782
1104	20-21	0.07740048179508108
1104	22-23	0.12529642600186008
1104	24-25	0.11623911553814992
1104	26-27	0.1598096214623297
1104	28-29	0.1713513245971825
1104	30-31	0.1595904897889966
1104	32-33	0.24825586765893348
1104	34-35	0.22199116907350458
1104	36-37	0.23513584901222373
1104	38-39	0.22935660233087418
1104	40-41	0.19608227803109912
1104	42-43	0.15248879387456782
1104	44-45	0.12739019532731533
1104	46-47	0.15188819739134374
1104	48-49	0.15690985409674596
1104	50-51	0.10032361514586796
1104	52-53	0.1111387982048484
1104	54-55	0.0964298588850383
1104	56-57	0.11005220681946781
1104	58-59	0.1357278327328686
1104	60-61	0.14102286883269244
1104	62-63	0.16929183483249943
1104	64-65	0.20440204747353619
1104	66-67	0.2785683398402341
1104	68-69	0.2736435526352494
1104	70-71	0.22376904632315586
1104	72-73	0.24181707692275012
1104	74-75	0.28169397727904766
1104	76-77	0.23766891564336134
1104	78-79	0.26744282198579583
1104	80-81	0.27641493168854936
1104	82-83	0.16093654592718565
1104	84-85	0.17543214560149067
1104	86-87	0.2089351645538784
1104	88-89	0.18109723212752016
1104	90-91	0.20298902294810972
1104	92-93	0.17263390786673938
1104	94-95	0.14052834678564352
1104	96-97	0.1416209792569667
1104	98-99	0.1019203540009741
1104	100	0.07800817923037684
1105	1	0.028521763648257092
1105	2	0.024612732373164192
1105	3	0.0433682003378415
1105	4	0.060092046857846526
1105	5	0.0813682384209713
1105	6	0.0991372208967718
1105	7	0.09398515610371305
1105	8	0.10361245233750793
1105	9	0.15873811399443838
1105	10-11	0.13247215094606446
1105	12-13	0.1777319210888706
1105	14-15	0.2171576701436777
1105	16-17	0.18639757187818162
1105	18-19	0.19528333851567226
1105	20-21	0.17206682029513587
1105	22-23	0.22490622390137816
1105	24-25	0.16816112526975502
1105	26-27	0.15171039303546507
1105	28-29	0.172813846552188
1105	30-31	0.2194868938024399
1105	32-33	0.2464192521143005
1105	34-35	0.2354284472748489
1105	36-37	0.2038242019319867
1105	38-39	0.1729789130817707
1105	40-41	0.2085054832268156
1105	42-43	0.23980648968700535
1105	44-45	0.2706399933576549
1105	46-47	0.17827145784115572
1105	48-49	0.1548143030984548
1105	50-51	0.1951042940909815
1105	52-53	0.19145207194487313
1105	54-55	0.10821763647747673
1105	56-57	0.11240960970231839
1105	58-59	0.08643521713590019
1105	60-61	0.04994027878039731
1105	62-63	0.053785689612773524
1105	64-65	0.13848095137522876
1105	66-67	0.0676874087192374
1105	68-69	0.1548356533049926
1105	70-71	0.16703702434798373
1105	72-73	0.1433606593029353
1105	74-75	0.1263882262953615
1105	76-77	0.10552862059867607
1105	78-79	0.07820873125263006
1105	80-81	0.026952372664894142
1105	82-83	0.011979238114534496
1105	84-85	-0.012845001464029338
1105	86-87	0.013338293802462431
1105	88-89	0.07454087741538018
1105	90-91	0.09832101635860369
1105	92-93	0.07257680578511838
1105	94-95	0.06635317885918823
1105	96-97	0.12173408226312787
1105	98-99	0.0679527650879912
1105	100	0.11203158917589917
1106	1	-0.02239180156582421
1106	2	0.0123316669563458
1106	3	-0.0051310954636143435
1106	4	-0.01610574232282147
1106	5	-0.013037706802144555
1106	6	0.028997835510672587
1106	7	-0.029704453285376076
1106	8	0.015277478494638785
1106	9	0.02562136722622199
1106	10-11	0.03877405042027959
1106	12-13	0.03384627908486948
1106	14-15	0.053572363440800075
1106	16-17	0.03029380582270136
1106	18-19	0.011401432653194377
1106	20-21	-0.020653278987758483
1106	22-23	0.06669955941929828
1106	24-25	0.038864226349971887
1106	26-27	0.11041951269626082
1106	28-29	0.06616298711551138
1106	30-31	0.04037081813295629
1106	32-33	0.023593630623913953
1106	34-35	0.057052669636085795
1106	36-37	0.0914227152538345
1106	38-39	0.13614063008498078
1106	40-41	0.13368200867312652
1106	42-43	0.13220181255914554
1106	44-45	0.11368000234426034
1106	46-47	0.13168336599579078
1106	48-49	0.1369266463501475
1106	50-51	0.11784125979791327
1106	52-53	0.07915285852058673
1106	54-55	0.10091219718501776
1106	56-57	0.09423594987242012
1106	58-59	0.10468017086236614
1106	60-61	0.11583608697856107
1106	62-63	0.040184019189275944
1106	64-65	0.02186322071503355
1106	66-67	0.032454958033824255
1106	68-69	0.10946304869716528
1106	70-71	0.06794918799180749
1106	72-73	0.03477413307877697
1106	74-75	0.07169246427143605
1106	76-77	0.06647924415779727
1106	78-79	0.1259213117058664
1106	80-81	0.08272470614386407
1106	82-83	0.19041547463256592
1106	84-85	0.14289342539282757
1106	86-87	0.14299083470969265
1106	88-89	0.12613541958702257
1106	90-91	0.10099036121398797
1106	92-93	0.044028474677745066
1106	94-95	0.1472416254518123
1106	96-97	0.09408174760911336
1106	98-99	0.0815399284835685
1106	100	0.08087799238459326
1107	1	0.06607231202264785
1107	2	0.09243254087310859
1107	3	0.07684080764263612
1107	4	0.061418783330069004
1107	5	0.04738441155807038
1107	6	0.015879478082375442
1107	7	0.016876817230638608
1107	8	0.02895417826831448
1107	9	-0.04687140786961663
1107	10-11	-0.05385202306231207
1107	12-13	-0.05154727512201873
1107	14-15	0.01113692668947408
1107	16-17	-0.012505414508808599
1107	18-19	0.01878916592462332
1107	20-21	0.08035228472597566
1107	22-23	0.052485969034130164
1107	24-25	0.0456324972546156
1107	26-27	0.08274976228892683
1107	28-29	0.1004149357890256
1107	30-31	0.11874007424455613
1107	32-33	0.08039886719623013
1107	34-35	0.09623234537745162
1107	36-37	0.053328915332507165
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2216	24-25	-0.2616769826235128
2216	26-27	-0.2389682986264532
2216	28-29	-0.24028475364937663
2216	30-31	-0.30148348110608225
2216	32-33	-0.303014004679234
2216	34-35	-0.28435429701192305
2216	36-37	-0.228180375329039
2216	38-39	-0.2293894117997155
2216	40-41	-0.24843413481945475
2216	42-43	-0.23216527636794382
2216	44-45	-0.21459169865901373
2216	46-47	-0.1668119436105684
2216	48-49	-0.15240681871421202
2216	50-51	-0.1642926962289195
2216	52-53	-0.18351681127685993
2216	54-55	-0.19085545525278036
2216	56-57	-0.2524076494421905
2216	58-59	-0.23161378715487047
2216	60-61	-0.1863140193197239
2216	62-63	-0.17265072304099505
2216	64-65	-0.15307371425983973
2216	66-67	-0.17928301526035284
2216	68-69	-0.16074732670740133
2216	70-71	-0.17325803668555295
2216	72-73	-0.23358208010625248
2216	74-75	-0.17435475769437403
2216	76-77	-0.28384594276563746
2216	78-79	-0.21295762724402323
2216	80-81	-0.16576402292100312
2216	82-83	-0.24001388677005764
2216	84-85	-0.312349715699753
2216	86-87	-0.24409742171003046
2216	88-89	-0.18805071346323388
2216	90-91	-0.2537077127246192
2216	92-93	-0.27586001308858243
2216	94-95	-0.2131311468996735
2216	96-97	-0.13761221358119968
2216	98-99	-0.10590829257070311
2216	100	-0.1718424575585793
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40648.0
3	5639.0
4	3815.0
5	3407.0
6	2789.0
7	3141.0
8	3658.0
9	3792.0
10	4110.0
11	4405.0
12	4833.0
13	5364.0
14	5899.0
15	6056.0
16	5965.0
17	6010.0
18	5961.0
19	5894.0
20	5839.0
21	6104.0
22	7129.0
23	9688.0
24	14955.0
25	20451.0
26	22400.0
27	19297.0
28	20486.0
29	25344.0
30	31331.0
31	37621.0
32	48157.0
33	64963.0
34	94600.0
35	149088.0
36	284341.0
37	610643.0
38	993563.0
39	458203.0
40	970.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.544492907584754	19.444438969620638	13.095888035035369	23.915180087759236
2	15.478565428814612	19.824379116679054	40.48267984945655	24.21437560504978
3	18.42426416506276	25.137354650446582	29.68920153462717	26.749179649863493
4	12.192327225923783	16.371315695935234	38.73498634390495	32.70137073423603
5	12.893455509937526	38.09195307146438	35.58137365240112	13.433217766196973
6	31.24602922751352	37.86594343583264	17.66610229958234	13.221925037071495
7	27.84153211898231	32.32718492846936	22.202913785457355	17.628369167090977
8	25.43208270548997	36.67019145896508	20.012706334212723	17.885019501332227
9	25.153728930538076	17.14717736305979	20.745644853402766	36.95344885299937
10-11	24.047238577284507	26.96549414461669	28.64214466728063	20.345122610818173
12-13	24.576928869782964	24.29976231839021	28.736496650924703	22.38681216090212
14-15	21.941852753251474	26.365933401374463	25.57519339000216	26.117020455371904
16-17	21.817669977475724	29.72719905685138	26.162437575366976	22.292693390305924
18-19	21.72412943576335	28.63513331515723	28.07970002857809	21.56103722050133
20-21	22.780274979831134	28.02253788724881	27.605002930069876	21.59218420285018
22-23	22.5276504886261	27.76286441652295	27.566182146669956	22.143302948180995
24-25	22.296613908135576	27.80937289254124	27.601456123632545	22.292557075690638
26-27	22.045089664426666	27.83316512100201	27.673504381595905	22.44824083297542
28-29	22.14145408917415	27.826601803979795	27.785920285903863	22.246023820942195
30-31	22.294090623826158	28.282074743904147	27.377902692034727	22.04593194023497
32-33	21.98042714708875	28.011089695632858	27.8213560404925	22.18712711678589
34-35	22.107419522732823	27.77808161897099	27.85423900345344	22.260259854842744
36-37	22.395935551524538	28.106134200047794	27.374244423659793	22.12368582476788
38-39	22.213229435995185	28.27858089672714	27.477441893197565	22.030747774080115
40-41	22.055668187748743	27.92061554552012	27.804267876875127	22.21944838985601
42-43	22.008582946583903	27.858310426204003	27.77855156863466	22.35455505857743
44-45	22.060817475140365	28.018474174081593	27.760766048490186	22.159942302287853
46-47	22.114030930908488	28.13311574986223	27.642370301991516	22.11048301723777
48-49	22.030709429948118	27.97086746797173	27.698770446699044	22.299652655381113
50-51	22.01145296095934	27.935490610983372	27.697935792851936	22.355120635205356
52-53	22.085187577326327	27.882584222045402	27.740579739664472	22.2916484609638
54-55	22.157309505697675	28.216148504730665	27.570122242183714	22.056419747387945
56-57	22.006698785752686	27.95457729827468	27.655019268330705	22.38370464764193
58-59	21.949499221833022	27.61076046829637	27.946271574114757	22.493468735755847
60-61	22.195125957389493	28.013573865381552	27.53694459911599	22.254355578112964
62-63	21.994913056297207	28.00044677064487	27.619246288066872	22.385393884991053
64-65	22.04084589191454	27.74712924228356	27.745338894034543	22.466685971767358
66-67	21.86882620033284	27.818931879686502	27.65367854160521	22.658563378375447
68-69	22.282053366774825	27.929559339349847	27.60094247192746	22.187444821947867
70-71	22.245278946765026	27.72377679002872	27.66601088042342	22.364933382782837
72-73	21.951995884601303	27.847679002843627	27.80462959358631	22.395695518968758
74-75	22.051003673387935	27.89725025602027	27.663822179444956	22.387923891146833
76-77	22.111508698692887	27.880952357488503	27.512118975373767	22.49541996844484
78-79	22.163917926063554	27.908827395996695	27.632710527819953	22.2945441501198
80-81	22.05490859517435	27.779215873068015	27.736724537227143	22.42915099453049
82-83	21.965580431707235	27.819771533746724	27.837296839088626	22.377351195457415
84-85	22.066665604535324	27.729101320267585	27.687760321411897	22.516472753785198
86-87	22.17883513499974	27.804961775326465	27.65191525775888	22.364287831914915
88-89	21.977084705453386	27.7215443515737	27.68818643641876	22.61318450655415
90-91	21.944484500576316	27.65372056134353	27.763317422928264	22.638477515151894
92-93	22.16610540727329	27.81127888306972	27.639337078858368	22.38327863079862
94-95	21.968656537470416	27.524401880870535	27.799355195798096	22.70758638586095
96-97	22.02616908140835	27.546909756940387	27.765806627083034	22.66111453456823
98-99	22.022157571154953	27.672180547941338	27.695830706906538	22.609831173997176
100	22.120235566400943	27.54759793943451	27.599857706279092	22.732308787885458
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2697.0
1	1646.0
2	548.0
3	467.0
4	392.5
5	307.0
6	250.5
7	251.5
8	278.5
9	275.0
10	263.5
11	265.5
12	269.0
13	332.5
14	383.0
15	401.0
16	521.5
17	712.0
18	986.5
19	1405.0
20	2005.5
21	2833.5
22	4207.0
23	6494.5
24	9659.5
25	13498.5
26	18504.0
27	24771.0
28	32480.5
29	40955.0
30	48678.0
31	56719.5
32	65027.5
33	73025.5
34	80364.5
35	85722.5
36	90470.0
37	95940.0
38	99887.5
39	100974.0
40	102552.0
41	105350.5
42	105881.5
43	106010.5
44	104934.0
45	103035.0
46	102997.0
47	101411.0
48	99654.0
49	97812.0
50	95473.0
51	93228.0
52	88026.0
53	83873.5
54	81571.5
55	77144.0
56	71426.0
57	65368.5
58	59829.0
59	55236.0
60	49918.0
61	43076.5
62	37089.0
63	31428.0
64	25493.5
65	20836.0
66	16989.0
67	13304.0
68	10393.0
69	8319.5
70	6255.0
71	4620.5
72	3669.0
73	2880.0
74	2215.0
75	1643.0
76	1135.0
77	828.0
78	617.5
79	395.0
80	271.5
81	196.0
82	139.0
83	121.5
84	98.5
85	70.0
86	53.0
87	40.5
88	36.5
89	25.0
90	14.5
91	15.0
92	10.5
93	5.5
94	7.0
95	6.0
96	2.5
97	2.0
98	1.5
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07618431154623954
2	0.0783835139907023
3	0.07982776634228977
4	0.08196132095258947
5	0.08012318159602358
6	0.08012318159602358
7	0.07907281624941451
8	0.07903999233233297
9	0.07884304882984378
10-11	0.07859686945173226
12-13	0.07748085627096013
14-15	0.07621713546332108
16-17	0.07510112228254893
18-19	0.0744774678579998
20-21	0.0742805243555106
22-23	0.07579042454126114
24-25	0.07588889629250574
26-27	0.0752160059923343
28-29	0.07152331532066177
30-31	0.07672590617808485
32-33	0.0752160059923343
34-35	0.07239314912332241
36-37	0.06930770091765825
38-39	0.07035806626426733
40-41	0.07336145467722766
42-43	0.07821939440529463
44-45	0.07935181954460753
46-47	0.08260138733567937
48-49	0.0856047757486397
50-51	0.08632690192443344
52-53	0.08668796501233031
54-55	0.0867372008879526
56-57	0.08450517452640832
58-59	0.08358610484812537
60-61	0.08120637085971419
62-63	0.08178078940864103
64-65	0.08069760014495042
66-67	0.08025447726434971
68-69	0.07997547396915668
70-71	0.0779896269857239
72-73	0.07820298244675387
74-75	0.07767779977344932
76-77	0.07766138781490856
78-79	0.07802245090280542
80-81	0.07907281624941451
82-83	0.07843274986632459
84-85	0.07764497585636779
86-87	0.07833427811508
88-89	0.07562630495585347
90-91	0.07286909592100466
92-93	0.07270497633559699
94-95	0.07300039158933078
96-97	0.07231108933061857
98-99	0.07162178707190638
100	0.07063706955946036
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3046559.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.74711800177075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.8297371495958	46.82267727819351
2	14.241732479881852	17.587718719698316
3	4.5453000176171905	8.41977529623778
4	1.9824601858136017	4.896448121089793
5	0.9875744903772349	3.0489939296430864
6	0.5843770018731409	2.16501574153091
7	0.3787658680401435	1.6371390524242506
8	0.2585107425997726	1.2769834662426833
9	0.18833044219645723	1.0465975833867278
>10	0.9471593926272763	10.076056131219037
>50	0.04520457687610616	1.8804917643426768
>100	0.010688132839641453	1.0268264943222245
>500	1.0634644119004218E-4	0.04878973586083933
>1k	5.317322059037918E-5	0.06648668580814866
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.2823917081533625E-5	0.0
9	0.0	0.0	0.0	9.847175124460087E-5	0.0
10-11	0.0	0.0	0.0	9.847175124460087E-5	0.0
12-13	0.0	0.0	0.0	9.847175124460087E-5	0.0
14-15	0.0	0.0	0.0	9.847175124460087E-5	0.0
16-17	0.0	0.0	0.0	2.2976741957073538E-4	0.0
18-19	0.0	0.0	0.0	2.461793781115022E-4	0.0
20-21	0.0	0.0	0.0	3.2823917081533624E-4	0.0
22-23	0.0	0.0	0.0	4.2671092205993715E-4	0.0
24-25	0.0	0.0	0.0	5.744185489268384E-4	0.0
26-27	0.0	0.0	0.0	7.221261757937397E-4	0.0
28-29	0.0	0.0	0.0	0.0019037871907289503	0.0
30-31	0.0	0.0	0.0	0.00628578012111369	0.0
32-33	0.0	0.0	0.0	0.014639467018363998	0.0
34-35	0.0	0.0	0.0	0.02619348583106383	0.0
36-37	0.0	0.0	0.0	0.048070626565905994	0.0
38-39	0.0	0.0	0.0	0.08885434353971153	0.0
40-41	0.0	0.0	0.0	0.1385169300840719	0.0
42-43	0.0	0.0	0.0	0.18883599497006295	0.0
44-45	0.0	0.0	0.0	0.2465240292408583	1.6411958540766813E-5
46-47	0.0	0.0	0.0	0.3200003676278713	3.2823917081533625E-5
48-49	0.0	0.0	0.0	0.4042757747347089	3.2823917081533625E-5
50-51	0.0	0.0	0.0	0.49839835696600654	3.2823917081533625E-5
52-53	0.0	0.0	0.0	0.5937354241293209	3.2823917081533625E-5
54-55	0.0	0.0	0.0	0.6956372747089421	3.2823917081533625E-5
56-57	0.0	0.0	0.0	0.8116862335507042	3.2823917081533625E-5
58-59	0.0	0.0	0.0	0.9519100073230159	3.2823917081533625E-5
60-61	0.0	0.0	0.0	1.1005038799511186	3.2823917081533625E-5
62-63	0.0	1.6411958540766813E-5	0.0	1.2511000115211948	3.2823917081533625E-5
64-65	0.0	3.2823917081533625E-5	0.0	1.4088681689735862	3.2823917081533625E-5
66-67	0.0	3.2823917081533625E-5	0.0	1.5853787830795332	3.2823917081533625E-5
68-69	0.0	3.2823917081533625E-5	0.0	1.7893958396998055	3.2823917081533625E-5
70-71	0.0	3.2823917081533625E-5	0.0	2.012696947605479	3.2823917081533625E-5
72-73	0.0	3.2823917081533625E-5	0.0	2.247256659070118	3.2823917081533625E-5
74-75	0.0	3.2823917081533625E-5	0.0	2.482554908669092	3.2823917081533625E-5
76-77	0.0	3.2823917081533625E-5	0.0	2.731983854571666	3.2823917081533625E-5
78-79	0.0	3.2823917081533625E-5	0.0	3.0209984444745697	3.2823917081533625E-5
80-81	0.0	3.2823917081533625E-5	0.0	3.3365511713378933	3.2823917081533625E-5
82-83	0.0	3.2823917081533625E-5	0.0	3.6656765879144304	3.2823917081533625E-5
84-85	0.0	3.2823917081533625E-5	0.0	3.988696755913803	3.2823917081533625E-5
86-87	0.0	3.2823917081533625E-5	0.0	4.328851008629736	3.2823917081533625E-5
88	0.0	3.2823917081533625E-5	0.0	4.608281014744832	3.2823917081533625E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4205	0.0	34.87446	1
TCAACGC	3940	0.0	34.595585	4
GGTATCA	2540	0.0	34.048893	1
ATCAACG	4140	0.0	32.924297	3
CAACGCA	4225	0.0	32.259796	5
AACGCAG	4365	0.0	31.331762	6
GTACATG	9125	0.0	28.536177	1
TATCAAC	5005	0.0	27.79755	2
TACATGG	9240	0.0	26.604101	2
ACATGGG	9330	0.0	25.440674	3
ACGCAGA	5335	0.0	25.370798	7
CGCAGAG	5490	0.0	24.397684	8
CATGGGG	6485	0.0	20.583881	4
GCAGAGT	6755	0.0	19.06344	9
GACGTAT	125	0.0064117494	18.80091	1
GAGTACA	6555	0.0	18.714865	1
CATGGGA	3325	0.0	16.963226	4
ACACCGT	655	0.0	16.502989	6
GTATTAG	1495	0.0	16.348618	1
AGTACAT	6820	0.0	16.333635	2
>>END_MODULE