FastQCFastQC Report
Wed 25 May 2016
SRR1033001_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033001_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3046559
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65940.21644090923563275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54360.17843081325521679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44050.054.60691
GTATCAA82750.043.8301281
ATCAACG97900.036.324923
TCAACGC98300.036.08144
CAACGCA99600.035.6553465
AACGCAG102950.034.4951176
TATCAAC104650.034.2510722
ACGCAGA121200.029.183067
CGCAGAG123450.028.4606698
GCAGAGT138550.025.0903029
GTACATG91200.025.0875131
TACATGG93850.023.4568672
ACATGGG95250.022.0259913
GAGTACT89900.021.4238612-13
CAGAGTA134250.021.1172110-11
GTATAGA13050.020.8805031
AGAGTAC125650.020.52501310-11
GTGGTAT21300.019.6306151
AGTACTT93050.019.36076712-13
TATAACG2451.9484833E-719.199612
Produced by FastQC (version 0.11.5)