##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033001_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3046559
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84350114342115	34.0	31.0	34.0	31.0	34.0
2	32.895408229415544	34.0	31.0	34.0	31.0	34.0
3	32.974723286173024	34.0	31.0	34.0	31.0	34.0
4	36.37269851002393	37.0	37.0	37.0	35.0	37.0
5	36.35423210251303	37.0	37.0	37.0	35.0	37.0
6	36.41194508296081	37.0	37.0	37.0	35.0	37.0
7	36.375929039943095	37.0	37.0	37.0	35.0	37.0
8	36.32094438348314	37.0	37.0	37.0	35.0	37.0
9	38.16513548564134	39.0	39.0	39.0	37.0	39.0
10-11	38.174973305949436	39.0	39.0	39.0	37.0	39.0
12-13	38.16607786030075	39.0	39.0	39.0	37.0	39.0
14-15	39.71877189970718	41.0	40.0	41.0	37.0	41.0
16-17	39.733383794635195	41.0	40.0	41.0	37.0	41.0
18-19	39.758887814087956	41.0	40.0	41.0	37.0	41.0
20-21	39.771993419461104	41.0	40.0	41.0	37.0	41.0
22-23	39.73550339908073	41.0	40.0	41.0	37.0	41.0
24-25	39.71549000035778	41.0	40.0	41.0	37.0	41.0
26-27	39.63999958641864	41.0	40.0	41.0	37.0	41.0
28-29	39.57840977312437	41.0	40.0	41.0	37.0	41.0
30-31	39.46807233997438	41.0	39.5	41.0	36.5	41.0
32-33	39.34463520975632	41.0	39.0	41.0	35.5	41.0
34-35	39.25415362052729	41.0	39.0	41.0	35.0	41.0
36-37	39.164423534879845	41.0	39.0	41.0	35.0	41.0
38-39	39.06323314270296	41.0	39.0	41.0	35.0	41.0
40-41	38.96227268206525	41.0	39.0	41.0	35.0	41.0
42-43	38.8276243788484	41.0	39.0	41.0	35.0	41.0
44-45	38.65621657089195	41.0	39.0	41.0	35.0	41.0
46-47	38.4359113018983	41.0	38.5	41.0	35.0	41.0
48-49	38.229418665451746	41.0	38.0	41.0	34.0	41.0
50-51	38.072783589617	40.5	38.0	41.0	34.0	41.0
52-53	37.92757189340499	40.0	38.0	41.0	34.0	41.0
54-55	37.78020103992734	40.0	37.0	41.0	34.0	41.0
56-57	37.57026796461187	40.0	37.0	41.0	33.0	41.0
58-59	37.399396499460536	40.0	36.5	41.0	33.0	41.0
60-61	37.20902007806184	40.0	36.0	41.0	33.0	41.0
62-63	36.970110541105555	39.0	35.5	41.0	33.0	41.0
64-65	36.68173552522698	39.0	35.0	41.0	32.0	41.0
66-67	36.40978165858597	38.5	35.0	41.0	32.0	41.0
68-69	36.115971822636624	37.5	35.0	40.5	32.0	41.0
70-71	35.79215616700678	37.0	35.0	40.0	31.0	41.0
72-73	35.45245997205372	36.5	35.0	39.0	31.0	41.0
74-75	35.109163978114324	36.0	35.0	39.0	31.0	41.0
76-77	34.76310617979169	36.0	35.0	39.0	31.0	40.5
78-79	34.42200233115459	35.0	35.0	37.0	31.0	39.5
80-81	34.09225719902356	35.0	34.5	37.0	30.0	39.0
82-83	33.78724045718465	35.0	34.0	36.5	30.0	39.0
84-85	33.509288019696974	35.0	34.0	36.0	30.0	37.5
86-87	33.36334484249279	35.0	34.0	36.0	30.0	37.0
88-89	33.17426644289509	35.0	34.0	35.5	30.0	37.0
90-91	32.997766988920944	35.0	34.0	35.0	30.0	36.0
92-93	32.835644082389344	35.0	34.0	35.0	30.0	36.0
94-95	32.74007511425185	35.0	34.0	35.0	29.5	36.0
96-97	32.67862234081139	35.0	34.0	35.0	30.0	36.0
98-99	32.56642001681241	35.0	34.0	35.0	30.0	36.0
100	32.48479021742234	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19597164920300258
1101	2	0.2569048388665607
1101	3	0.22805354476355433
1101	4	0.1558640452555764
1101	5	0.17036909138331424
1101	6	0.1782662960328807
1101	7	0.13046528689138626
1101	8	0.062248643993989106
1101	9	0.11445754142255282
1101	10-11	0.17740303961816295
1101	12-13	0.23779552253749614
1101	14-15	0.34287402542184253
1101	16-17	0.3408606208272005
1101	18-19	0.31256132932204395
1101	20-21	0.31969765503369985
1101	22-23	0.32219796196307016
1101	24-25	0.3092254364962628
1101	26-27	0.30214803305544535
1101	28-29	0.33455167537037767
1101	30-31	0.3066964021626717
1101	32-33	0.32249998415115044
1101	34-35	0.3245429047768198
1101	36-37	0.3436846968369238
1101	38-39	0.41199241332405023
1101	40-41	0.3790270881742259
1101	42-43	0.4131118734651622
1101	44-45	0.3226303027876867
1101	46-47	0.321766363802773
1101	48-49	0.3528873424959542
1101	50-51	0.34409241736253193
1101	52-53	0.38250874592966255
1101	54-55	0.34365264140107854
1101	56-57	0.43618600715571887
1101	58-59	0.4343182818156919
1101	60-61	0.412465824420984
1101	62-63	0.38128585160445994
1101	64-65	0.3865901392111084
1101	66-67	0.41206917851837943
1101	68-69	0.36525236829091057
1101	70-71	0.4339850592072523
1101	72-73	0.39747836853609186
1101	74-75	0.37922641422385794
1101	76-77	0.36717675547738793
1101	78-79	0.3610525088067007
1101	80-81	0.3737202403930837
1101	82-83	0.3930500911290693
1101	84-85	0.3808255436119623
1101	86-87	0.3692551472689729
1101	88-89	0.33696825588604895
1101	90-91	0.3190171330941993
1101	92-93	0.31269329927688716
1101	94-95	0.3059201410844423
1101	96-97	0.28103960612075696
1101	98-99	0.340119482174039
1101	100	0.2950475299020283
1102	1	0.21032630824075227
1102	2	0.23641082241282163
1102	3	0.19412804480125345
1102	4	0.12282519320361729
1102	5	0.12488873012895851
1102	6	0.16228331411146257
1102	7	0.12472748049623306
1102	8	0.05585157095588045
1102	9	0.13565379818845713
1102	10-11	0.1576320235506259
1102	12-13	0.17278103756713392
1102	14-15	0.26743968959917197
1102	16-17	0.2522895503094986
1102	18-19	0.2676766589588411
1102	20-21	0.28124863921603804
1102	22-23	0.27628173394908373
1102	24-25	0.208868963590497
1102	26-27	0.2649901232113052
1102	28-29	0.25800078250708225
1102	30-31	0.3623435435952729
1102	32-33	0.3437494840292743
1102	34-35	0.3207482033923199
1102	36-37	0.32408911445427435
1102	38-39	0.2913269003318746
1102	40-41	0.3063468273771406
1102	42-43	0.3289239908138626
1102	44-45	0.3736238382207091
1102	46-47	0.3959176828131916
1102	48-49	0.3701202578239844
1102	50-51	0.3654915983223219
1102	52-53	0.3277929007857807
1102	54-55	0.31105265794594317
1102	56-57	0.34996635470274384
1102	58-59	0.3835186334667071
1102	60-61	0.4359569383193431
1102	62-63	0.42766009677669103
1102	64-65	0.39757281873693273
1102	66-67	0.4213223258498786
1102	68-69	0.43215030070761173
1102	70-71	0.40698565079036797
1102	72-73	0.45482072642422366
1102	74-75	0.43080935248908503
1102	76-77	0.4406026726318686
1102	78-79	0.4039575781387086
1102	80-81	0.36673246899303535
1102	82-83	0.33934463278423976
1102	84-85	0.39475918708458124
1102	86-87	0.41868090213465337
1102	88-89	0.35097391185287563
1102	90-91	0.35604495279412873
1102	92-93	0.33973375386752025
1102	94-95	0.33505783712573844
1102	96-97	0.32894794808726857
1102	98-99	0.41641477650038894
1102	100	0.4239286604988237
1103	1	0.12911884864546863
1103	2	0.19427400019115026
1103	3	0.1900628755751157
1103	4	0.07918325158313877
1103	5	0.10379079193593554
1103	6	0.08737161349474576
1103	7	0.11471545331425403
1103	8	0.041498239956681005
1103	9	0.08491889684212595
1103	10-11	0.1321818726699746
1103	12-13	0.15679302147928809
1103	14-15	0.1911635879175293
1103	16-17	0.2341786924182543
1103	18-19	0.244250438727029
1103	20-21	0.21053374559123483
1103	22-23	0.17517676898087586
1103	24-25	0.15394462940486875
1103	26-27	0.2326848747343533
1103	28-29	0.2126243609639502
1103	30-31	0.2530683527128943
1103	32-33	0.24213884111591
1103	34-35	0.2511313271869682
1103	36-37	0.21081935728633994
1103	38-39	0.20276762387641867
1103	40-41	0.2106023838163722
1103	42-43	0.2140601978118113
1103	44-45	0.20506615848259457
1103	46-47	0.18611798247194145
1103	48-49	0.18921690584501505
1103	50-51	0.1926671790292218
1103	52-53	0.22928162640727834
1103	54-55	0.20104514057630496
1103	56-57	0.2665283358267416
1103	58-59	0.2543430728711229
1103	60-61	0.23790398885636677
1103	62-63	0.2633200971484513
1103	64-65	0.24542180584221285
1103	66-67	0.22039427960113755
1103	68-69	0.20435708901123206
1103	70-71	0.18020522715443832
1103	72-73	0.1930934622013254
1103	74-75	0.09185389816053657
1103	76-77	0.13863807039274434
1103	78-79	0.1755167817667953
1103	80-81	0.198384465057309
1103	82-83	0.23158373328388393
1103	84-85	0.19331970373843177
1103	86-87	0.2491345374547791
1103	88-89	0.264687580819853
1103	90-91	0.23735357845014704
1103	92-93	0.27128982500433096
1103	94-95	0.2243749144138647
1103	96-97	0.26033072663750545
1103	98-99	0.22610502247713526
1103	100	0.2437077832976584
1104	1	0.11628820903757742
1104	2	0.1881174921389075
1104	3	0.16846619947607167
1104	4	0.10381391318062327
1104	5	0.08578015591582044
1104	6	0.0669536954465002
1104	7	0.11479415291901773
1104	8	0.040269715979341925
1104	9	0.1128598605650808
1104	10-11	0.1124749997640464
1104	12-13	0.1623132779474048
1104	14-15	0.24711524552795794
1104	16-17	0.21844658355818325
1104	18-19	0.21236060660023526
1104	20-21	0.2044902235524546
1104	22-23	0.21478133240060515
1104	24-25	0.19201758856787876
1104	26-27	0.22324150083600358
1104	28-29	0.21209545332604307
1104	30-31	0.2139547938126043
1104	32-33	0.20341299350381092
1104	34-35	0.19018412536298968
1104	36-37	0.17687523400572758
1104	38-39	0.1937773513790333
1104	40-41	0.24845110697315675
1104	42-43	0.27942021956993557
1104	44-45	0.22659021104930588
1104	46-47	0.25900567929328844
1104	48-49	0.26304697256608023
1104	50-51	0.25637508776082996
1104	52-53	0.23011549501365636
1104	54-55	0.27086818220029585
1104	56-57	0.22905386979777376
1104	58-59	0.2781870277577383
1104	60-61	0.29055836449652617
1104	62-63	0.25977152618281707
1104	64-65	0.3004529384799852
1104	66-67	0.36090258646015627
1104	68-69	0.3395670607140957
1104	70-71	0.27179116885886145
1104	72-73	0.3039087264526117
1104	74-75	0.3300302952231817
1104	76-77	0.38782344268237523
1104	78-79	0.32259028488295627
1104	80-81	0.3945733209618041
1104	82-83	0.33558728633956747
1104	84-85	0.2900856418550859
1104	86-87	0.2990671219845211
1104	88-89	0.3003290983334068
1104	90-91	0.3098424245171927
1104	92-93	0.29896821559741227
1104	94-95	0.32084920105403114
1104	96-97	0.2975947001574042
1104	98-99	0.2922984788364644
1104	100	0.34585090084362236
1105	1	0.024155213247048835
1105	2	0.07943972013947587
1105	3	0.03900555930040639
1105	4	0.00946939056493079
1105	5	-0.003168915023771035
1105	6	0.05761457504205936
1105	7	0.019629540837037496
1105	8	-0.03122034580538724
1105	9	0.001020186856457883
1105	10-11	0.023259715921732038
1105	12-13	0.014029665672453007
1105	14-15	0.055634221953376084
1105	16-17	0.07803210148905748
1105	18-19	0.09455745859927589
1105	20-21	0.05329533183108026
1105	22-23	0.09636025538175375
1105	24-25	0.1051842110792407
1105	26-27	0.048303072961246585
1105	28-29	0.05996558656605089
1105	30-31	0.09109103856167167
1105	32-33	0.12042802375291473
1105	34-35	0.10448731097773845
1105	36-37	0.1041268549416614
1105	38-39	0.11938208831696073
1105	40-41	0.1410212403221891
1105	42-43	0.07661085166681403
1105	44-45	0.12498617405478996
1105	46-47	0.10879144427583753
1105	48-49	0.13325434988246343
1105	50-51	0.1462195847555421
1105	52-53	0.16743349064609703
1105	54-55	0.14808377022362862
1105	56-57	0.18922657156846157
1105	58-59	0.19864418238962855
1105	60-61	0.1694535986668413
1105	62-63	0.1599242981004778
1105	64-65	0.12925858098333975
1105	66-67	0.19349509183238922
1105	68-69	0.0890963433157026
1105	70-71	0.06126555169303316
1105	72-73	0.10669435406308736
1105	74-75	0.1124976666303894
1105	76-77	0.1132784059026406
1105	78-79	0.10375022304371129
1105	80-81	0.11388587186306154
1105	82-83	0.12063188320159668
1105	84-85	0.12194775845344452
1105	86-87	0.14127589046569256
1105	88-89	0.11467076993347547
1105	90-91	0.10767617051187273
1105	92-93	0.1450736900925449
1105	94-95	0.09492401104991899
1105	96-97	0.09943815704927772
1105	98-99	0.16204846711622878
1105	100	0.13457150894399206
1106	1	0.07795285836077426
1106	2	0.1420076264769321
1106	3	0.08319650054327354
1106	4	0.068626257617872
1106	5	0.09420675586808613
1106	6	0.032470973567129136
1106	7	0.05694249585604183
1106	8	0.01307542875508716
1106	9	0.026815544359280352
1106	10-11	0.03230680020048027
1106	12-13	0.0669554409942208
1106	14-15	0.060769606399453835
1106	16-17	0.06269129442456034
1106	18-19	0.08338981403996826
1106	20-21	0.04216091638207331
1106	22-23	0.09121783722518018
1106	24-25	0.08622298254653771
1106	26-27	0.049975492789357645
1106	28-29	0.0782684391080295
1106	30-31	0.14600647474274098
1106	32-33	0.1188855206953221
1106	34-35	0.09244138353749776
1106	36-37	0.06448625892986115
1106	38-39	0.07917778695473743
1106	40-41	0.09407851499934594
1106	42-43	0.04643377697046702
1106	44-45	0.09130090694755921
1106	46-47	0.11241633939080486
1106	48-49	-0.0061442502850184155
1106	50-51	0.04005229291612977
1106	52-53	0.03264616005508003
1106	54-55	0.025299802780608616
1106	56-57	0.006362484990923178
1106	58-59	-0.02119849936558893
1106	60-61	-0.042530152841266045
1106	62-63	-0.02687714066568958
1106	64-65	-0.04991060954415616
1106	66-67	-0.07055337436359821
1106	68-69	-0.024155705149048856
1106	70-71	0.0336574104627374
1106	72-73	-0.06435785696312024
1106	74-75	0.023393199686346122
1106	76-77	0.05549505184055192
1106	78-79	-0.026278155909430723
1106	80-81	-0.0557986444990064
1106	82-83	-0.01431117167126672
1106	84-85	-0.03530017508378336
1106	86-87	-0.11218187644706745
1106	88-89	-0.13391090303042574
1106	90-91	-0.12081686321849361
1106	92-93	-0.16939202511996143
1106	94-95	-0.1525380607005502
1106	96-97	-0.12697835466887852
1106	98-99	-0.1385752937094793
1106	100	-0.1309171870557293
1107	1	0.05205120863782753
1107	2	0.05962754316258412
1107	3	8.860120854592424E-5
1107	4	0.04575268687074896
1107	5	0.05526289988358002
1107	6	0.058454995957362144
1107	7	0.03157027094392362
1107	8	0.03465333468361109
1107	9	0.09361738847177037
1107	10-11	0.0780604867132979
1107	12-13	0.07462112602424043
1107	14-15	0.07642185657186218
1107	16-17	0.12062125386634648
1107	18-19	0.08889123811656674
1107	20-21	0.08872715575276402
1107	22-23	0.07771454311484405
1107	24-25	0.08072496785224104
1107	26-27	0.1259801805158247
1107	28-29	0.15200377229376727
1107	30-31	0.18181399764184647
1107	32-33	0.15531300368126466
1107	34-35	0.1568940637002072
1107	36-37	0.1366275441306115
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2216	22-23	-0.28430244021765816
2216	24-25	-0.2799969524918069
2216	26-27	-0.2951434761894234
2216	28-29	-0.2371385376361559
2216	30-31	-0.2626066788402426
2216	32-33	-0.3503107301628532
2216	34-35	-0.34194901312672243
2216	36-37	-0.36603400408987596
2216	38-39	-0.34060925229918126
2216	40-41	-0.2736519878477779
2216	42-43	-0.2626926047629681
2216	44-45	-0.3352136632727678
2216	46-47	-0.365664904515441
2216	48-49	-0.3934845324297953
2216	50-51	-0.3309275190642751
2216	52-53	-0.32711227818230526
2216	54-55	-0.29496393811120214
2216	56-57	-0.35961685463279736
2216	58-59	-0.38762646965976444
2216	60-61	-0.4037561419777873
2216	62-63	-0.37467693210760444
2216	64-65	-0.3532147264751515
2216	66-67	-0.3657068686491627
2216	68-69	-0.3093343785695737
2216	70-71	-0.35311400640537016
2216	72-73	-0.2973309311901815
2216	74-75	-0.35092922088561807
2216	76-77	-0.36487659190299837
2216	78-79	-0.370829467546578
2216	80-81	-0.46252675238959284
2216	82-83	-0.373471231354074
2216	84-85	-0.4044267803753314
2216	86-87	-0.3418086101042519
2216	88-89	-0.2983611231270089
2216	90-91	-0.29568600960147506
2216	92-93	-0.29949701089879
2216	94-95	-0.3457421822469371
2216	96-97	-0.3550649531527199
2216	98-99	-0.3063698892279305
2216	100	-0.2789108971397809
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12.0
3	5.0
4	12.0
5	24.0
6	78.0
7	162.0
8	355.0
9	686.0
10	1184.0
11	1866.0
12	2983.0
13	4751.0
14	5692.0
15	7712.0
16	9424.0
17	13547.0
18	16790.0
19	9401.0
20	6302.0
21	5988.0
22	5951.0
23	6722.0
24	7148.0
25	8731.0
26	10683.0
27	13587.0
28	17540.0
29	22334.0
30	28490.0
31	36048.0
32	46437.0
33	60725.0
34	85072.0
35	132555.0
36	254649.0
37	589403.0
38	1069843.0
39	561391.0
40	2276.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.38483212453193	19.551170112757273	13.393766838752368	23.670230923958428
2	15.752047397876288	19.92857494543008	40.217327797016296	24.10204985967734
3	18.506823451560976	25.17728238349452	29.946903909731393	26.368990255213102
4	12.308472086945589	16.532549938005275	38.52027442472278	32.638703550326355
5	12.622864669889774	37.810659491758365	35.622441567376534	13.944034270975331
6	31.19610719524849	38.01320951234572	17.281312849978057	13.509370442427738
7	27.552066425496978	32.541561068766185	22.06820784620985	17.838164659526985
8	25.58718269323057	36.098423442079145	19.80926423352674	18.505129631163545
9	25.388887101782565	16.207836235119878	20.939375299112363	37.463901363985194
10-11	24.19477187213509	26.53176912050612	28.518945472580704	20.754513534778088
12-13	25.141614803467455	23.818793447618646	28.935225721236417	22.10436602767748
14-15	22.147491182663696	25.70953092919708	26.362415909117022	25.78056197902221
16-17	21.3488890252905	29.29063576316756	26.880359119912008	22.480116091629935
18-19	21.161501708814807	27.694682126309182	29.763116121942286	21.380700042933725
20-21	22.22770016927294	26.994783294858234	29.536421254274085	21.241095281594742
22-23	21.861264189397	26.81231870904593	29.550002904922383	21.77641419663469
24-25	21.645907895291828	26.926137768917464	29.562174313283236	21.865780022507476
26-27	21.4204798921216	26.94041773336533	29.6297949739249	22.00930740058817
28-29	21.524646380657053	26.99554331054244	29.69654036616973	21.78326994263077
30-31	21.741345649738175	27.31844404386291	29.295987987368875	21.644222319030035
32-33	21.447673519610223	27.137127922524957	29.66501742098509	21.750181136879732
34-35	21.61451305235717	26.867040294699933	29.657972756015642	21.860473896927253
36-37	21.911311502835595	27.204927828277942	29.13828512061894	21.745475548267525
38-39	21.756332867865392	27.433104375644945	29.15354613509735	21.657016621392316
40-41	21.578424252256518	27.110849991863738	29.367606657643165	21.943119098236576
42-43	21.635989072800808	27.100968200825548	29.189183951587662	22.073858774785986
44-45	21.746547857329563	27.318820114652752	29.023611540323724	21.91102048769396
46-47	21.918754884987454	27.520973920061437	28.6813602269641	21.878910967987014
48-49	21.890350024718586	27.44195239540275	28.544718461950218	22.122979117928445
50-51	21.903256896666736	27.493671901005957	28.374479531725605	22.228591670601705
52-53	22.00054282572994	27.48173549140515	28.297978467036483	22.21974321582843
54-55	22.010561094497298	27.84219840589547	28.17192248625232	21.97531801335491
56-57	21.774993995717658	27.556304726446214	28.321649487245786	22.347051790590346
58-59	21.743171302741334	27.166443186956997	28.68477468300951	22.40561082729215
60-61	21.95991357651342	27.56561307174125	28.310903121492416	22.16357023025292
62-63	21.741511545526777	27.509864451164294	28.32763529628013	22.420988707028798
64-65	21.794595737222135	27.26837959428312	28.492712103298214	22.44431256519653
66-67	21.648135371130305	27.34709137328093	28.353653532937827	22.65111972265094
68-69	22.05329197078735	27.43773174730224	28.286952826904738	22.222023455005676
70-71	22.020404334924145	27.248713589448553	28.278547744939086	22.452334330688217
72-73	21.816646255660896	27.419360662307867	28.290376125983446	22.47361695604779
74-75	21.89465229460516	27.520802978048348	28.135791888487965	22.44875283885853
76-77	21.92627612088006	27.53143958901803	27.95252148572963	22.589762804372278
78-79	22.02653879343876	27.566608754335633	28.032347313805513	22.3745051384201
80-81	21.926027485840134	27.417582606951697	28.16767685288657	22.4887130543216
82-83	21.780907507450635	27.466840808802473	28.243505324951624	22.508746358795275
84-85	21.83474864593136	27.462753880689654	28.001345124122	22.70115234925698
86-87	21.91283020769241	27.509360182514737	27.98724523834103	22.590564371451823
88-89	21.65879301275786	27.467604776218362	28.03952992768055	22.83407228334322
90-91	21.660401388126417	27.35626581193004	28.106343153449675	22.876989646493865
92-93	21.82550819342812	27.488530382300898	27.927374351187435	22.758587073083547
94-95	21.55764000662394	27.276712438481148	28.06367450395541	23.101973050939506
96-97	21.590653958967383	27.31034953613134	28.012356263736844	23.08664024116444
98-99	21.52788137828307	27.38181459505865	27.969444867052445	23.120859159605832
100	21.70051523702643	27.23127961743068	27.82568793186017	23.242517213682714
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	87.0
1	80.0
2	200.5
3	347.0
4	233.0
5	151.5
6	406.5
7	975.0
8	1791.5
9	2078.0
10	1581.5
11	1200.0
12	1264.0
13	1423.5
14	1596.0
15	1889.0
16	2143.5
17	2413.0
18	2804.0
19	3293.0
20	3894.0
21	4713.5
22	5972.5
23	8096.5
24	11033.5
25	14529.5
26	19297.5
27	25185.5
28	32559.5
29	40747.5
30	48710.5
31	56545.5
32	64311.5
33	72220.5
34	79380.5
35	85426.5
36	90355.5
37	94930.0
38	98815.5
39	100692.5
40	102392.5
41	104689.5
42	105356.5
43	105196.0
44	103889.0
45	102452.0
46	102410.0
47	100766.0
48	99006.0
49	97238.5
50	94807.5
51	92345.0
52	87235.5
53	83272.5
54	81098.0
55	76829.0
56	70917.5
57	64774.0
58	59392.5
59	55004.5
60	49864.0
61	42887.5
62	36788.5
63	30949.5
64	25089.5
65	20356.0
66	16263.5
67	12805.0
68	9972.5
69	7774.5
70	5743.5
71	4191.5
72	3213.5
73	2480.0
74	1953.0
75	1365.0
76	865.0
77	608.0
78	421.5
79	255.5
80	157.0
81	78.0
82	33.5
83	15.0
84	8.0
85	4.5
86	3.5
87	3.5
88	2.5
89	0.5
90	1.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.923587562230044E-4
2	2.954152537338026E-4
3	2.2976741957073538E-4
4	0.010897540471069164
5	5.25182673304538E-4
6	0.0038732222156209676
7	5.908305074676052E-4
8	0.1390092888402949
9	0.0014770762686690132
10-11	0.0
12-13	8.205979270383406E-5
14-15	1.312956683261345E-4
16-17	0.0
18-19	9.847175124460087E-5
20-21	0.0
22-23	1.9694350248920174E-4
24-25	5.087707147637711E-4
26-27	0.005448770235534582
28-29	0.007861328141027303
30-31	0.014179932179222527
32-33	0.017068436882397484
34-35	0.024585113894068686
36-37	0.03543341848951555
38-39	0.040586773471316326
40-41	0.050795011683673286
42-43	0.06715773434881779
44-45	0.10488882703404069
46-47	0.11273374321652722
48-49	0.1084009861617648
50-51	0.11114178323807286
52-53	0.10585713258794593
54-55	0.11188032137240736
56-57	0.09512371170228445
58-59	0.08726238356125714
60-61	0.0889692272494969
62-63	0.08778756623456167
64-65	3.2823917081533625E-5
66-67	3.2823917081533625E-5
68-69	8.205979270383406E-5
70-71	1.6411958540766813E-5
72-73	0.0
74-75	0.0
76-77	6.564783416306725E-5
78-79	0.0
80-81	2.790032951930358E-4
82-83	1.8053154394843493E-4
84-85	0.0
86-87	3.4465112935610305E-4
88-89	2.1335546102996858E-4
90-91	2.1335546102996858E-4
92-93	0.001050365346609076
94-95	0.0010996012222313763
96-97	2.2976741957073538E-4
98-99	1.6411958540766813E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3046559.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.97394691093844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8355273359099	44.881819434959425
2	14.79099125020443	17.741482479998307
3	4.729804940446327	8.509952111922669
4	2.010899809911013	4.824063937712771
5	1.0797786335761892	3.2379293222832004
6	0.6187925852587918	2.2266860194315874
7	0.40069120944211667	1.6821723325932851
8	0.2725467772818209	1.307656476115783
9	0.20515890017112054	1.107377008845237
>10	0.9909582061844054	10.396254402896641
>50	0.050042144469712106	1.9992083378945333
>100	0.014205836008220443	1.4010294452440697
>500	3.83327087033652E-4	0.14513030269264146
>1k	1.095220245285992E-4	0.1441450321177914
>5k	1.095220245285992E-4	0.39509335529210077
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6594	0.21644090923563275	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5436	0.17843081325521679	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.2823917081533625E-5	0.0
5	0.0	0.0	0.0	3.2823917081533625E-5	0.0
6	0.0	0.0	0.0	3.2823917081533625E-5	0.0
7	0.0	0.0	0.0	6.564783416306725E-5	0.0
8	0.0	0.0	0.0	2.2976741957073538E-4	0.0
9	0.0	0.0	0.0	3.6106308789686986E-4	0.0
10-11	0.0	0.0	0.0	3.6106308789686986E-4	0.0
12-13	0.0	0.0	0.0	3.7747504643763667E-4	0.0
14-15	0.0	0.0	0.0	3.938870049784035E-4	0.0
16-17	0.0	0.0	0.0	5.908305074676052E-4	0.0
18-19	0.0	0.0	0.0	6.072424660083721E-4	0.0
20-21	0.0	0.0	0.0	6.236544245491389E-4	0.0
22-23	0.0	0.0	0.0	7.221261757937397E-4	0.0
24-25	0.0	0.0	0.0	8.534218441198743E-4	0.0
26-27	0.0	0.0	0.0	9.847175124460088E-4	0.0
28-29	0.0	0.0	0.0	0.0021827904859219862	0.0
30-31	0.0	0.0	0.0	0.006564783416306725	0.0
32-33	0.0	0.0	0.0	0.014967706189179333	0.0
34-35	0.0	0.0	0.0	0.0265053130433384	0.0
36-37	0.0	0.0	0.0	0.048480925529425165	0.0
38-39	0.0	0.0	0.0	0.0896585295082091	0.0
40-41	0.0	0.0	0.0	0.13969859109900712	0.0
42-43	0.0	0.0	0.0	0.19013253969478353	0.0
44-45	0.0	0.0	0.0	0.24818163705347573	0.0
46-47	0.0	0.0	0.0	0.3219533906942226	0.0
48-49	0.0	0.0	0.0	0.4062123858425194	0.0
50-51	0.0	0.0	0.0	0.5007945029129586	0.0
52-53	0.0	0.0	0.0	0.5965254570812513	0.0
54-55	0.0	0.0	0.0	0.6988211946658509	0.0
56-57	0.0	0.0	0.0	0.8153132763882137	0.0
58-59	0.0	0.0	0.0	0.9562755882948599	0.0
60-61	0.0	0.0	0.0	1.1058705903939494	0.0
62-63	0.0	0.0	0.0	1.2575334992691753	0.0
64-65	0.0	0.0	0.0	1.415990958980279	0.0
66-67	0.0	0.0	0.0	1.593354994930346	0.0
68-69	0.0	0.0	0.0	1.7986850082338797	0.0
70-71	0.0	0.0	0.0	2.0235616641594665	0.0
72-73	0.0	0.0	0.0	2.260287754151487	0.0
74-75	0.0	0.0	0.0	2.4978672659876273	0.0
76-77	0.0	0.0	0.0	2.7494790023761233	0.0
78-79	0.0	0.0	0.0	3.039740901128125	0.0
80-81	0.0	0.0	0.0	3.356852764052822	0.0
82-83	0.0	0.0	0.0	3.687832731944466	0.0
84-85	0.0	0.0	0.0	4.012412036005211	0.0
86-87	0.0	0.0	0.0	4.354978846626636	0.0
88	0.0	0.0	0.0	4.63627981601538	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4405	0.0	54.6069	1
GTATCAA	8275	0.0	43.830128	1
ATCAACG	9790	0.0	36.32492	3
TCAACGC	9830	0.0	36.0814	4
CAACGCA	9960	0.0	35.655346	5
AACGCAG	10295	0.0	34.495117	6
TATCAAC	10465	0.0	34.251072	2
ACGCAGA	12120	0.0	29.18306	7
CGCAGAG	12345	0.0	28.460669	8
GCAGAGT	13855	0.0	25.090302	9
GTACATG	9120	0.0	25.087513	1
TACATGG	9385	0.0	23.456867	2
ACATGGG	9525	0.0	22.025991	3
GAGTACT	8990	0.0	21.42386	12-13
CAGAGTA	13425	0.0	21.11721	10-11
GTATAGA	1305	0.0	20.880503	1
AGAGTAC	12565	0.0	20.525013	10-11
GTGGTAT	2130	0.0	19.630615	1
AGTACTT	9305	0.0	19.360767	12-13
TATAACG	245	1.9484833E-7	19.19961	2
>>END_MODULE