FastQCFastQC Report
Wed 25 May 2016
SRR1033000_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033000_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003552
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT88430.8811700838621218No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66980.6674292911578075No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55190.5499465897133382No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41310.41163786231306404No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27560.2746245336564523No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23260.23177672905838462No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA23020.2293852236854692No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22970.22888699339944518No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22600.22520008928286728No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22520.2244029208252288No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG20720.20646663052836328No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC18480.18414591371448613No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA16290.16232342718663306No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC15870.15813829278403113No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC14430.14378926054653868No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA13390.1334260705972386No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG12600.12555403207805874No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT11070.11030818532572303No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC10810.10771738783839802No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCGT851.7551132E-533.17494294
GTACATG45600.026.0768491
TACATGG45550.024.5577222
GTACAAG5000.024.440091
CGCCGTA5100.023.95967994
CATGGGG18150.023.5648254
ATGGGCG1050.002335239422.3810355
CTGCTCG1050.00233591622.379929
GTACATA6100.022.3443451
ACATGGG48500.021.5134813
GTACAAA9750.020.2462311
GTAGACT2401.5793012E-719.5834064
CGTATCA2702.5958798E-819.14726494
CATGGGC13050.019.0881944
CCGTATC3252.3646862E-1018.79913194
GAGTACT8300.018.68588412-13
ACAACAC11050.018.289665
GTACTGG3106.361006E-918.1936151
GTCTTGC6300.017.9048291
GAGAACA13150.017.1559581
Produced by FastQC (version 0.11.5)