FastQCFastQC Report
Wed 25 May 2016
SRR1032999_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032999_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5015593
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76220.1519660785873176No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG265400.028.4064881
TACATGG256050.027.8651312
ACATGGG277800.024.9051823
GAGTACA183150.022.0958331
CATGGGA64050.022.088484
CATGGGG186150.020.5784684
AGTACAT188400.020.257662
GTATCAA201700.019.3180271
GCGCTAG2001.4085321E-518.8007051
GGTATCA159000.018.7711451
TCAACGC216250.017.4750484
CATGGGT42050.017.4372124
AACGCAG217700.017.4223826
GTATAGA11950.017.306091
CAACGCA219000.017.298025
ATCAACG223400.016.9366233
CGACTAT1255.0477393E-616.9213152-53
TAGACCG2002.994429E-416.4502895
AGAGTAC158850.016.05086710-11
TATCAAC240900.016.037962
Produced by FastQC (version 0.11.5)