##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032999_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5015593
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.936093897571034	34.0	31.0	34.0	30.0	34.0
2	31.96104927174115	34.0	31.0	34.0	30.0	34.0
3	32.091785756938414	34.0	31.0	34.0	30.0	34.0
4	35.41080905089388	37.0	35.0	37.0	35.0	37.0
5	35.4985877043851	37.0	37.0	37.0	35.0	37.0
6	35.55301656254804	37.0	37.0	37.0	35.0	37.0
7	35.53298443474181	37.0	37.0	37.0	35.0	37.0
8	35.51002523530119	37.0	37.0	37.0	35.0	37.0
9	37.18573397004103	39.0	39.0	39.0	35.0	39.0
10-11	37.19546661780571	39.0	39.0	39.0	35.0	39.0
12-13	37.157121401198225	39.0	39.0	39.0	35.0	39.0
14-15	38.67208673032282	41.0	40.0	41.0	36.0	41.0
16-17	38.69630769482292	41.0	40.0	41.0	36.0	41.0
18-19	38.72124562339887	41.0	40.0	41.0	36.0	41.0
20-21	38.76565283506855	41.0	40.0	41.0	36.0	41.0
22-23	38.76385753389479	41.0	39.5	41.0	35.0	41.0
24-25	38.79755065851635	41.0	39.0	41.0	35.0	41.0
26-27	38.775580773798836	41.0	39.0	41.0	35.0	41.0
28-29	38.74754530521117	41.0	39.0	41.0	35.0	41.0
30-31	38.69242320898047	41.0	39.0	41.0	35.0	41.0
32-33	38.63500327877482	41.0	39.0	41.0	35.0	41.0
34-35	38.54158012821216	41.0	39.0	41.0	34.5	41.0
36-37	38.43490859405857	41.0	39.0	41.0	34.0	41.0
38-39	38.32346753414801	41.0	39.0	41.0	34.0	41.0
40-41	38.22189759017528	41.0	38.0	41.0	34.0	41.0
42-43	38.086303354359096	40.5	38.0	41.0	33.0	41.0
44-45	37.92961968804087	40.0	38.0	41.0	33.0	41.0
46-47	37.77580208362201	40.0	38.0	41.0	33.0	41.0
48-49	37.60072009431387	40.0	37.0	41.0	33.0	41.0
50-51	37.42127560988302	40.0	37.0	41.0	32.5	41.0
52-53	37.23842873614346	40.0	36.0	41.0	32.0	41.0
54-55	37.02987862053401	40.0	36.0	41.0	32.0	41.0
56-57	36.79218539064075	39.0	35.0	41.0	31.0	41.0
58-59	36.533550369816695	39.0	35.0	41.0	31.0	41.0
60-61	36.23194276329838	38.5	35.0	41.0	31.0	41.0
62-63	35.93211371018342	38.0	35.0	40.0	31.0	41.0
64-65	35.61545703568851	37.0	35.0	40.0	30.0	41.0
66-67	35.2689804575451	37.0	35.0	39.5	30.0	41.0
68-69	34.91744824590033	36.0	34.5	39.0	30.0	41.0
70-71	34.55562522716656	35.5	34.0	39.0	29.5	41.0
72-73	34.245399995573806	35.0	34.0	38.0	29.0	40.0
74-75	33.977135206146116	35.0	34.0	37.0	29.0	39.5
76-77	33.68045981801155	35.0	34.0	37.0	29.0	39.0
78-79	33.35471069921343	35.0	34.0	36.0	29.0	39.0
80-81	33.08870107682182	35.0	34.0	36.0	29.0	37.5
82-83	32.83673774965393	35.0	34.0	35.5	28.5	37.0
84-85	32.74020978177455	35.0	34.0	35.0	29.0	37.0
86-87	32.58937298142014	35.0	34.0	35.0	28.5	36.0
88-89	32.41372077040541	35.0	34.0	35.0	28.0	36.0
90-91	32.271264035977396	35.0	34.0	35.0	27.0	36.0
92-93	32.12749668882623	35.0	33.0	35.0	27.0	35.5
94-95	31.985214111272583	35.0	33.0	35.0	27.0	35.0
96-97	31.8422322146155	35.0	33.0	35.0	27.0	35.0
98-99	31.682921042437055	35.0	33.0	35.0	26.0	35.0
100	31.557853278764846	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3546145461035337
1101	2	0.4391031851872107
1101	3	0.4065710767982722
1101	4	0.3848830788215807
1101	5	0.3369596799303949
1101	6	0.3474048140191712
1101	7	0.3342599042674621
1101	8	0.34395695051729547
1101	9	0.40085312222420555
1101	10-11	0.38707783556409225
1101	12-13	0.22840259242082084
1101	14-15	0.2948204674513093
1101	16-17	0.2977825231609472
1101	18-19	0.3081210860793746
1101	20-21	0.2868582706433287
1101	22-23	0.2663207085292498
1101	24-25	0.2966307957362204
1101	26-27	0.36399236315025263
1101	28-29	0.3592641845149984
1101	30-31	0.34720874037520133
1101	32-33	0.3038686548394196
1101	34-35	0.34903407499545835
1101	36-37	0.3760520307040309
1101	38-39	0.3764070361388576
1101	40-41	0.3750857110614021
1101	42-43	0.40467867768670374
1101	44-45	0.3836583861715468
1101	46-47	0.3014449195241937
1101	48-49	0.3537152621362267
1101	50-51	0.3252398046499678
1101	52-53	0.3849431682444333
1101	54-55	0.4184230914565603
1101	56-57	0.4271523111187321
1101	58-59	0.3570516739380949
1101	60-61	0.3770014950272298
1101	62-63	0.38445706154439563
1101	64-65	0.33825417952724024
1101	66-67	0.37975302336969463
1101	68-69	0.3834113869478202
1101	70-71	0.3969637645850099
1101	72-73	0.32394588253928447
1101	74-75	0.2730469475681403
1101	76-77	0.29518226711844164
1101	78-79	0.32043896910095526
1101	80-81	0.31199129702909545
1101	82-83	0.257884655828704
1101	84-85	0.22084862507612257
1101	86-87	0.2201059241816381
1101	88-89	0.2565693036001875
1101	90-91	0.26946150284810955
1101	92-93	0.2716054423418086
1101	94-95	0.2520065298676073
1101	96-97	0.23017454144030935
1101	98-99	0.29363505709318716
1101	100	0.3467351059834165
1102	1	0.271429715401176
1102	2	0.26318737717037166
1102	3	0.3140891826959731
1102	4	0.29456872874952467
1102	5	0.24813760019887354
1102	6	0.251939679446437
1102	7	0.22641838954434235
1102	8	0.22938689210254637
1102	9	0.2473659479664434
1102	10-11	0.22891902764922634
1102	12-13	0.24988590338124084
1102	14-15	0.3088896522173812
1102	16-17	0.31428976542716214
1102	18-19	0.3319557351330218
1102	20-21	0.31319482682738453
1102	22-23	0.3371422308418843
1102	24-25	0.35567303642267234
1102	26-27	0.3859985677554931
1102	28-29	0.418079120517703
1102	30-31	0.40865207543038196
1102	32-33	0.4423252546436842
1102	34-35	0.47875397897303884
1102	36-37	0.4603170114061186
1102	38-39	0.46488086837787534
1102	40-41	0.41875268216310246
1102	42-43	0.45517112782780345
1102	44-45	0.4368227459859071
1102	46-47	0.4457748494632696
1102	48-49	0.48088424568540233
1102	50-51	0.46109526338936746
1102	52-53	0.4279337744082099
1102	54-55	0.472660171495491
1102	56-57	0.4673789248333904
1102	58-59	0.39537935321254025
1102	60-61	0.48499515761476886
1102	62-63	0.44445681450436325
1102	64-65	0.4588769971468736
1102	66-67	0.4860004566145619
1102	68-69	0.4642384713758858
1102	70-71	0.470780600752704
1102	72-73	0.42778295741451444
1102	74-75	0.4161519397640916
1102	76-77	0.4336582075561637
1102	78-79	0.4298137804543174
1102	80-81	0.47740131009993547
1102	82-83	0.46179769086771216
1102	84-85	0.4189675035218414
1102	86-87	0.38136025504401516
1102	88-89	0.3989277891371117
1102	90-91	0.3969224069068602
1102	92-93	0.4226344987065005
1102	94-95	0.42756847162448963
1102	96-97	0.42229220474630225
1102	98-99	0.421429929545301
1102	100	0.4865199638416051
1103	1	0.2260179194787284
1103	2	0.29536149114188603
1103	3	0.3370396428352649
1103	4	0.23821122254732785
1103	5	0.24580156890635863
1103	6	0.19131350643163358
1103	7	0.2201869917197925
1103	8	0.23881629900418488
1103	9	0.2543024784732424
1103	10-11	0.28752583114291497
1103	12-13	0.2791096760761391
1103	14-15	0.31666797396317037
1103	16-17	0.36234163084047566
1103	18-19	0.36312372798982295
1103	20-21	0.35388784177320787
1103	22-23	0.32313410876795956
1103	24-25	0.37834089609519594
1103	26-27	0.4123839468069832
1103	28-29	0.4136214464197536
1103	30-31	0.394119952190529
1103	32-33	0.43014461260920456
1103	34-35	0.4011579478780334
1103	36-37	0.37747654481562165
1103	38-39	0.3951017447112335
1103	40-41	0.44680206039739545
1103	42-43	0.40328480182959225
1103	44-45	0.3485705983139553
1103	46-47	0.33689482269504367
1103	48-49	0.393398572323683
1103	50-51	0.41316919802571306
1103	52-53	0.35016236501822817
1103	54-55	0.363317038337172
1103	56-57	0.35118888853693164
1103	58-59	0.3521303886918403
1103	60-61	0.35263297251314185
1103	62-63	0.329636692823847
1103	64-65	0.339479914188729
1103	66-67	0.2875294349537185
1103	68-69	0.31012220572102933
1103	70-71	0.3148526177677695
1103	72-73	0.2832288919470116
1103	74-75	0.31205270587869194
1103	76-77	0.2742873070056575
1103	78-79	0.26520842038242876
1103	80-81	0.26706339246577215
1103	82-83	0.2639525638327811
1103	84-85	0.2725281353475353
1103	86-87	0.2662140206692598
1103	88-89	0.26681590826360235
1103	90-91	0.29243308227159304
1103	92-93	0.28250717399733105
1103	94-95	0.3223848951566666
1103	96-97	0.29873387823081643
1103	98-99	0.30145884266638845
1103	100	0.31905041558632874
1104	1	0.06873718451255684
1104	2	0.08280711512437122
1104	3	0.08930060428886577
1104	4	0.07251470520679959
1104	5	0.11039256583080004
1104	6	0.08483377385972801
1104	7	0.03816131363745967
1104	8	0.05461637701332478
1104	9	0.0614523047396176
1104	10-11	0.12899485493464624
1104	12-13	0.06712566512349838
1104	14-15	0.15908982304916464
1104	16-17	0.14247345723234162
1104	18-19	0.13673505367410854
1104	20-21	0.10404620686791333
1104	22-23	0.11415905055059739
1104	24-25	0.16257279176123518
1104	26-27	0.15816709436415266
1104	28-29	0.17416901430522103
1104	30-31	0.14355033611026613
1104	32-33	0.200621591722431
1104	34-35	0.21212416416732793
1104	36-37	0.2063191859525233
1104	38-39	0.21611550994358453
1104	40-41	0.1861290880522688
1104	42-43	0.15110805599373123
1104	44-45	0.1480068710588398
1104	46-47	0.12377132460839135
1104	48-49	0.13768246515773086
1104	50-51	0.15896230017274604
1104	52-53	0.12037907994535146
1104	54-55	0.10171926653346475
1104	56-57	0.13172924619716753
1104	58-59	0.0999781840540308
1104	60-61	0.19606004394483278
1104	62-63	0.13644316541928703
1104	64-65	0.14582069895929806
1104	66-67	0.14634256269710733
1104	68-69	0.13783672729186947
1104	70-71	0.09437449446101454
1104	72-73	0.08355134629118766
1104	74-75	0.08418105264738784
1104	76-77	0.09144300467399091
1104	78-79	0.09218839521101785
1104	80-81	0.061517408921801575
1104	82-83	0.09968455669684317
1104	84-85	0.07070751629188265
1104	86-87	0.11376058185565796
1104	88-89	0.15286570751766249
1104	90-91	0.1016139210905962
1104	92-93	0.07249525547419466
1104	94-95	0.10867596732166618
1104	96-97	0.0876586990221675
1104	98-99	0.1190353315782211
1104	100	0.12762235647234021
1105	1	0.031063157371740147
1105	2	0.047069631055737915
1105	3	0.1004890471969162
1105	4	0.09609370919389448
1105	5	0.12070312579908915
1105	6	0.1626474805750746
1105	7	0.13531519042140872
1105	8	0.13478363825169737
1105	9	0.21850577132988747
1105	10-11	0.17707996299104423
1105	12-13	0.21409534579873934
1105	14-15	0.22886866572922315
1105	16-17	0.2204465628829908
1105	18-19	0.23101522517688267
1105	20-21	0.16625603288882473
1105	22-23	0.15853686180147974
1105	24-25	0.1504704709873721
1105	26-27	0.20682275410079143
1105	28-29	0.22707429239331134
1105	30-31	0.18513405627535917
1105	32-33	0.2220034806727469
1105	34-35	0.22560239801147475
1105	36-37	0.1982504136535823
1105	38-39	0.21995754695303305
1105	40-41	0.19778780246198835
1105	42-43	0.21752172421016525
1105	44-45	0.19929710256158728
1105	46-47	0.22942181973525066
1105	48-49	0.23999161189379947
1105	50-51	0.22983069160764558
1105	52-53	0.2239504768442373
1105	54-55	0.18538560978084462
1105	56-57	0.18773683948130326
1105	58-59	0.19814581760112304
1105	60-61	0.2327009525033148
1105	62-63	0.21390573099716903
1105	64-65	0.21085490276658447
1105	66-67	0.13734887729862066
1105	68-69	0.1630051058978097
1105	70-71	0.15701804529586383
1105	72-73	0.1750079526020798
1105	74-75	0.2308241305321772
1105	76-77	0.2051422682306452
1105	78-79	0.2346097136935228
1105	80-81	0.21525979375780935
1105	82-83	0.18622068270138925
1105	84-85	0.16309505492072418
1105	86-87	0.1799844239877757
1105	88-89	0.19310431294662322
1105	90-91	0.15938015944706052
1105	92-93	0.1948248743746035
1105	94-95	0.21030065870129278
1105	96-97	0.256725391921929
1105	98-99	0.28295265335989583
1105	100	0.2758241209941765
1106	1	0.10830680134021264
1106	2	0.12119462570511175
1106	3	0.1898169099467566
1106	4	0.20473202053625528
1106	5	0.18555042182595116
1106	6	0.15472183835488806
1106	7	0.15023144626962903
1106	8	0.23060944202108402
1106	9	0.24656999149882353
1106	10-11	0.23066143089119606
1106	12-13	0.3031244229193675
1106	14-15	0.3010909332477354
1106	16-17	0.24509381993994594
1106	18-19	0.2615317862918829
1106	20-21	0.26557837024665787
1106	22-23	0.28375193423137546
1106	24-25	0.29570914429235984
1106	26-27	0.298553282869257
1106	28-29	0.2741750318627396
1106	30-31	0.2999447038318195
1106	32-33	0.29443384688038776
1106	34-35	0.2909260222367962
1106	36-37	0.3273010435022172
1106	38-39	0.3215060080532268
1106	40-41	0.3100857517707567
1106	42-43	0.2947921496079644
1106	44-45	0.3157765785499933
1106	46-47	0.315350611406231
1106	48-49	0.3378361012872588
1106	50-51	0.2948954475561649
1106	52-53	0.27053329102550094
1106	54-55	0.2799120518277505
1106	56-57	0.2945188712598181
1106	58-59	0.2890674490616263
1106	60-61	0.34581349890276414
1106	62-63	0.33474429510791026
1106	64-65	0.36622956382768024
1106	66-67	0.3765697388958955
1106	68-69	0.34745958648944963
1106	70-71	0.33178968972998035
1106	72-73	0.3094793777795246
1106	74-75	0.3355961207279492
1106	76-77	0.3394400010231706
1106	78-79	0.31181234564934357
1106	80-81	0.24509764664135503
1106	82-83	0.2894065101879164
1106	84-85	0.3269942128552046
1106	86-87	0.31817448554467376
1106	88-89	0.2894213360686422
1106	90-91	0.23819048872162085
1106	92-93	0.2997692735605
1106	94-95	0.29658657384367615
1106	96-97	0.2768172203284216
1106	98-99	0.24780492442888402
1106	100	0.21311865987005518
1107	1	0.011803491904853303
1107	2	0.025511284731013717
1107	3	0.039618463722732145
1107	4	-0.008984060950702144
1107	5	-0.04181711864450932
1107	6	-0.05338270737264139
1107	7	0.024531469362429448
1107	8	-0.03844767214582845
1107	9	-0.015470939508915649
1107	10-11	0.0048691761218719876
1107	12-13	0.0027342791829454427
1107	14-15	0.07161262552737213
1107	16-17	0.05052158074363433
1107	18-19	0.10725276569218778
1107	20-21	0.09731459225830719
1107	22-23	0.14186586353983444
1107	24-25	0.1453290302901138
1107	26-27	0.19151522562804502
1107	28-29	0.1447583276743103
1107	30-31	0.14044038557177174
1107	32-33	0.1296014566974577
1107	34-35	0.15520494447761024
1107	36-37	0.17747155975244766
1107	38-39	0.1598400918217493
1107	40-41	0.15136887892556672
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2216	60-61	-0.35047791619441426
2216	62-63	-0.36201533962820776
2216	64-65	-0.3519589105347549
2216	66-67	-0.3565176570970152
2216	68-69	-0.3588953592916937
2216	70-71	-0.3839540548256082
2216	72-73	-0.3747930231696799
2216	74-75	-0.3258923446230497
2216	76-77	-0.33800022306142097
2216	78-79	-0.30539425673568843
2216	80-81	-0.3222929394029208
2216	82-83	-0.30234201043327147
2216	84-85	-0.3029166489392736
2216	86-87	-0.3116646601703863
2216	88-89	-0.29812710044573265
2216	90-91	-0.2949768838415281
2216	92-93	-0.3180035479227783
2216	94-95	-0.33344894626686283
2216	96-97	-0.33432205154685946
2216	98-99	-0.331252102995947
2216	100	-0.3829553541277235
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	44062.0
3	6920.0
4	4837.0
5	4404.0
6	4301.0
7	6856.0
8	8831.0
9	8221.0
10	7253.0
11	7171.0
12	7553.0
13	8028.0
14	8573.0
15	8821.0
16	9091.0
17	8661.0
18	8295.0
19	7593.0
20	7497.0
21	8064.0
22	10156.0
23	15965.0
24	25871.0
25	34108.0
26	32892.0
27	26694.0
28	29370.0
29	37826.0
30	47267.0
31	59225.0
32	79496.0
33	111369.0
34	169950.0
35	290831.0
36	609291.0
37	1237551.0
38	1578713.0
39	433724.0
40	262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.41651028525765	18.414656654265897	12.751764092035073	24.417068968441384
2	16.52587488047863	20.040721071039194	38.26537575603752	25.168028292444657
3	18.75993320298244	24.859520939516138	29.33254263457925	27.048003222922173
4	13.103410019002308	16.441135253597878	36.85916603362229	33.596288693777524
5	13.737881086793246	38.299676669351115	34.010537179033875	13.951905064821768
6	31.611405794524817	36.5065159920209	17.562339958572053	14.31973825488223
7	29.27885638963875	32.29675253814273	21.0257646353876	17.39862643683092
8	26.857555610538665	34.50146059972384	19.506967779010466	19.134016010727027
9	27.070924302408855	16.440050849632225	19.41801873598637	37.07100611197255
10-11	25.433382588553894	25.808268990770806	27.235742549137743	21.522605871537557
12-13	25.753133285279233	23.795748459234247	27.003743730311747	23.447374525174776
14-15	23.39919809010163	25.00178080264816	24.522949408244767	27.076071699005443
16-17	23.31058121943988	28.188059400116767	25.072997113636063	23.428362266807294
18-19	23.32894341858467	27.5242766342639	26.457159224013648	22.689620723137775
20-21	24.702347104219218	26.15287123908443	25.918338395308105	23.226443261388248
22-23	24.575647656039482	25.984237750046912	25.789696264873317	23.650418329040285
24-25	24.032084361118663	26.192810449365624	25.714478117837196	24.060627071678518
26-27	23.710791606770826	26.566078567579815	25.891823248834182	23.831306576815177
28-29	23.591723349754247	26.624183323096247	26.118188065910168	23.66590526123934
30-31	23.746474913535803	26.457769845761657	25.838675522244547	23.95707971845799
32-33	23.86228547325676	26.379800975528777	25.929757219196283	23.82815633201818
34-35	24.24059135963454	26.179697007696866	25.78968113471425	23.79003049795435
36-37	24.333199258919638	26.176323416216064	25.57966234795423	23.91081497691007
38-39	23.907665995682798	26.413131331874045	25.83585176743296	23.843350905010194
40-41	23.61703146511303	26.35302978910194	26.01778766535645	24.01215108042858
42-43	23.79849383478714	26.40156929788645	25.72903871525408	24.070898152072324
44-45	23.52010751009279	26.386863735247417	25.85590794198885	24.237120812670945
46-47	24.010397746529684	26.28406173397608	25.837107092618822	23.868433426875406
48-49	23.897114726488685	26.214861982196275	25.780923550927803	24.107099740387245
50-51	23.366070915441693	26.47836952912479	25.98019182385921	24.17536773157431
52-53	23.67347704399871	26.329501255126093	25.781723699847714	24.215298001027488
54-55	23.411575026607657	26.51724007534654	25.961899092750496	24.10928580529531
56-57	23.637299148553296	26.37559158175447	25.82273689360937	24.164372376082866
58-59	23.893722313126073	25.768388075188568	25.89055952428463	24.447330087400726
60-61	23.85468083752053	26.185177432656488	25.994336633671267	23.965805096151716
62-63	23.493788863254423	26.18347227426937	25.993059538076764	24.32967932439945
64-65	23.73383622839875	26.42557661194736	25.6737981081972	24.166789051456686
66-67	23.51399852879686	26.09637607727518	25.733733382992007	24.65589201093595
68-69	23.714912664025576	26.204134595852874	25.713758932836562	24.36719380728499
70-71	23.82393446652891	26.069504204987243	25.913797562009293	24.192763766474553
72-73	23.747297554804643	25.938156281395504	25.956104408415165	24.35844175538469
74-75	23.78758462714174	26.117297757842167	26.007963381613784	24.087154233402305
76-77	23.78999326973133	25.885505220098544	25.888737664582806	24.435763845587324
78-79	23.778410213290353	25.932294769843	25.9503326837822	24.338962333084446
80-81	23.66747701812082	26.179403571086862	25.968493367851835	24.18462604294048
82-83	23.639039948152764	25.903385833716108	26.162242903828403	24.295331314302725
84-85	23.71557484380261	25.976589721525933	25.964368289124174	24.343467145547283
86-87	23.931508729866398	25.901939203207885	25.93686784740981	24.229684219515903
88-89	23.5292873521386	25.976398043971532	25.959428052423604	24.534886551466265
90-91	23.717775477264272	25.855906249186315	25.87985912397523	24.546459149574183
92-93	23.761389581292786	25.830254510889723	26.085894706766478	24.322461201051013
94-95	23.725977095157045	25.568498393676105	26.13567742992965	24.5698470812372
96-97	23.75883737161195	25.647920950591324	26.114625154181383	24.47861652361534
98-99	23.4428373716437	25.763658309251845	26.1463915483243	24.64711277078015
100	23.519017911369726	25.524619653743848	26.071964711975948	24.88439772291048
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4886.0
1	2943.5
2	955.5
3	825.0
4	709.5
5	632.5
6	565.5
7	522.5
8	499.5
9	507.0
10	517.0
11	513.5
12	503.5
13	523.0
14	562.5
15	616.0
16	703.0
17	812.0
18	957.5
19	1163.0
20	1475.0
21	1828.0
22	2503.5
23	3796.0
24	5567.0
25	7569.0
26	10171.5
27	13983.0
28	20131.0
29	26848.0
30	32080.0
31	39030.5
32	46849.5
33	53252.0
34	62638.5
35	74770.5
36	87584.0
37	102001.5
38	116852.5
39	130078.5
40	148450.0
41	166302.0
42	175361.0
43	189268.0
44	196488.0
45	193961.5
46	197189.5
47	202639.5
48	208538.0
49	208628.5
50	201632.5
51	194045.5
52	184936.5
53	179705.5
54	173393.5
55	161919.0
56	147627.5
57	131042.0
58	119389.0
59	113744.0
60	105895.0
61	97533.5
62	91369.5
63	81284.5
64	67640.5
65	54172.5
66	44104.5
67	34439.0
68	25008.5
69	17715.5
70	11905.5
71	8315.0
72	6759.0
73	5000.0
74	3900.5
75	3229.0
76	2121.0
77	1547.0
78	1113.5
79	588.5
80	346.0
81	227.0
82	141.5
83	105.5
84	84.0
85	62.0
86	48.5
87	40.0
88	35.0
89	32.0
90	21.0
91	13.5
92	14.5
93	11.0
94	7.0
95	5.5
96	3.0
97	2.5
98	2.0
99	0.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07592322582793301
2	0.07849520485414187
3	0.07959178505911466
4	0.08166531853760861
5	0.0798509767439264
6	0.07975128763438341
7	0.0788740234704052
8	0.0788142100046794
9	0.078834147826588
10-11	0.07858492505273056
12-13	0.07736871791630621
14-15	0.07592322582793301
16-17	0.07478676997914305
18-19	0.07429829334238244
20-21	0.0740690083904336
22-23	0.07593319473888731
24-25	0.07587338127316152
26-27	0.07511574404063488
28-29	0.07156681174090482
30-31	0.07649145375232799
32-33	0.07470701869150866
34-35	0.0721250707543455
36-37	0.06880542340656429
38-39	0.07077926777551527
40-41	0.07363037630844448
42-43	0.07825595099123872
44-45	0.07916312188807983
46-47	0.0821936708181864
48-49	0.08539369123451604
50-51	0.08631083104231144
52-53	0.08659992945998608
54-55	0.08667968074762047
56-57	0.08453636489244643
58-59	0.0835992872627424
60-61	0.08146594031852265
62-63	0.08196438586623754
64-65	0.08128649992134529
66-67	0.08071827199695031
68-69	0.0802896088259155
70-71	0.07825595099123872
72-73	0.0783157644569645
74-75	0.07781731890924962
76-77	0.0778771323749754
78-79	0.07829582663505592
80-81	0.07938243792907439
82-83	0.07869458307322783
84-85	0.0778671634640211
86-87	0.07869458307322783
88-89	0.07586341236220723
90-91	0.07314189967168389
92-93	0.0730920551169124
94-95	0.07344096700031283
96-97	0.07255373392538031
98-99	0.0721051329324369
100	0.07139734025468175
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5015593.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.197564251036283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.15972934196202	12.554751272113032
2	11.415072445714818	4.6111331830511775
3	5.5222625974182575	3.346087608673493
4	3.295655439547601	2.662568499581596
5	2.431063829975273	2.455078395214796
6	1.841214187147451	2.231282510689811
7	1.4503474000825978	2.050543935964418
8	1.2152027633258853	1.9635308712249124
9	0.9844561490568912	1.7895254690613878
>10	8.323441366808614	34.16841993975119
>50	0.8975516819057285	12.461376163162912
>100	0.4414548223185192	15.630324629139212
>500	0.01740545427631812	2.364857916264621
>1k	0.00504362583582952	1.5582754513391834
>5k	9.889462423195125E-5	0.1522441547684678
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	7622	0.1519660785873176	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.990673286289378E-5	0.0
12-13	0.0	0.0	0.0	5.981346572578756E-5	0.0
14-15	0.0	0.0	0.0	1.2959584240587304E-4	0.0
16-17	0.0	0.0	0.0	1.6947148622306474E-4	0.0
18-19	0.0	0.0	0.0	2.3925386290315023E-4	0.0
20-21	0.0	0.0	0.0	2.591916848117461E-4	0.0
22-23	0.0	0.0	0.0	4.486009929434067E-4	0.0
24-25	0.0	0.0	0.0	9.869221844754947E-4	0.0
26-27	0.0	0.0	0.0	0.0019937821908595854	0.0
28-29	0.0	0.0	0.0	0.005662341422041223	0.0
30-31	0.0	0.0	0.0	0.017834381697238992	0.0
32-33	0.0	0.0	0.0	0.04384327037700228	0.0
34-35	0.0	0.0	0.0	0.08160550507188283	0.0
36-37	0.0	0.0	0.0	0.14391119853624487	0.0
38-39	0.0	0.0	0.0	0.24143705440214147	0.0
40-41	0.0	0.0	0.0	0.3492109507290564	0.0
42-43	0.0	0.0	0.0	0.46166026629353696	0.0
44-45	0.0	0.0	0.0	0.5952835487249464	0.0
46-47	0.0	0.0	0.0	0.7613556363125955	0.0
48-49	0.0	0.0	0.0	0.9348146869173795	0.0
50-51	0.0	0.0	0.0	1.115720514005024	0.0
52-53	0.0	0.0	0.0	1.300055646460947	0.0
54-55	0.0	0.0	0.0	1.509422714323112	0.0
56-57	0.0	0.0	0.0	1.7554554366751849	0.0
58-59	0.0	0.0	0.0	2.023070851243313	0.0
60-61	0.0	0.0	0.0	2.3045729587707777	0.0
62-63	0.0	0.0	0.0	2.5882283510643704	0.0
64-65	0.0	0.0	0.0	2.896586704702714	0.0
66-67	0.0	0.0	0.0	3.2473727433625497	0.0
68-69	0.0	0.0	0.0	3.6410649747696833	0.0
70-71	0.0	0.0	0.0	4.048524272204703	0.0
72-73	0.0	0.0	0.0	4.4704384905234535	0.0
74-75	0.0	0.0	0.0	4.913666240462494	0.0
76-77	0.0	0.0	0.0	5.381267977684792	0.0
78-79	0.0	0.0	0.0	5.911045812529046	0.0
80-81	0.0	0.0	0.0	6.463542795438146	0.0
82-83	0.0	0.0	0.0	7.03288723786001	0.0
84-85	0.0	0.0	0.0	7.60987783498382	0.0
86-87	0.0	0.0	0.0	8.21843997309989	0.0
88	0.0	0.0	0.0	8.709139677003298	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	26540	0.0	28.406488	1
TACATGG	25605	0.0	27.865131	2
ACATGGG	27780	0.0	24.905182	3
GAGTACA	18315	0.0	22.095833	1
CATGGGA	6405	0.0	22.08848	4
CATGGGG	18615	0.0	20.578468	4
AGTACAT	18840	0.0	20.25766	2
GTATCAA	20170	0.0	19.318027	1
GCGCTAG	200	1.4085321E-5	18.800705	1
GGTATCA	15900	0.0	18.771145	1
TCAACGC	21625	0.0	17.475048	4
CATGGGT	4205	0.0	17.437212	4
AACGCAG	21770	0.0	17.422382	6
GTATAGA	1195	0.0	17.30609	1
CAACGCA	21900	0.0	17.29802	5
ATCAACG	22340	0.0	16.936623	3
CGACTAT	125	5.0477393E-6	16.92131	52-53
TAGACCG	200	2.994429E-4	16.450289	5
AGAGTAC	15885	0.0	16.050867	10-11
TATCAAC	24090	0.0	16.03796	2
>>END_MODULE