FastQCFastQC Report
Wed 25 May 2016
SRR1032999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032999_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5015593
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119040.23733983199992503No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102690.20474149317937082No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT77990.15549507306513904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52990.10565051829364942No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA185750.029.2881871
GTATCAA270650.029.1964951
GTACATG249900.027.1464921
TACATGG242000.026.747252
TCAACGC313800.024.8399074
CAACGCA315200.024.7576965
ATCAACG317200.024.6033123
AACGCAG318400.024.5088736
TATCAAC330750.023.7231432
ACATGGG266600.023.591293
ACGCAGA358550.021.6721327
CGCAGAG365600.021.2154148
GTACTTT171750.020.92328514-15
AGTACTT169950.020.4401612-13
AGAGTAC283350.020.39989310-11
GCAGAGT379300.020.274019
GAGTACA176450.020.2616961
CATGGGG177100.019.8418624
GTATAGA12550.019.8401491
GAGTACT182800.019.76138912-13
Produced by FastQC (version 0.11.5)