##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032999_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5015593 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.829879936430245 34.0 31.0 34.0 31.0 34.0 2 32.87382728223761 34.0 31.0 34.0 31.0 34.0 3 32.94533846745539 34.0 31.0 34.0 31.0 34.0 4 36.35446436742375 37.0 37.0 37.0 35.0 37.0 5 36.34344054631227 37.0 37.0 37.0 35.0 37.0 6 36.39774459371006 37.0 37.0 37.0 35.0 37.0 7 36.364542138885675 37.0 37.0 37.0 35.0 37.0 8 36.30747151931985 37.0 37.0 37.0 35.0 37.0 9 38.153110310186655 39.0 39.0 39.0 37.0 39.0 10-11 38.15722866668009 39.0 39.0 39.0 37.0 39.0 12-13 38.15435891628368 39.0 39.0 39.0 37.0 39.0 14-15 39.68763155224118 41.0 40.0 41.0 37.0 41.0 16-17 39.697611528686636 41.0 40.0 41.0 37.0 41.0 18-19 39.72458730203986 41.0 40.0 41.0 37.0 41.0 20-21 39.72810941796912 41.0 40.0 41.0 37.0 41.0 22-23 39.684080327091934 41.0 40.0 41.0 37.0 41.0 24-25 39.65461691169918 41.0 40.0 41.0 37.0 41.0 26-27 39.574974285194195 41.0 40.0 41.0 37.0 41.0 28-29 39.506825214884856 41.0 40.0 41.0 36.0 41.0 30-31 39.38157880832835 41.0 39.0 41.0 36.0 41.0 32-33 39.24473387294384 41.0 39.0 41.0 35.0 41.0 34-35 39.13401516032103 41.0 39.0 41.0 35.0 41.0 36-37 39.03319916508377 41.0 39.0 41.0 35.0 41.0 38-39 38.92062733160366 41.0 39.0 41.0 35.0 41.0 40-41 38.801365860427666 41.0 39.0 41.0 35.0 41.0 42-43 38.633190432317775 41.0 38.0 41.0 35.0 41.0 44-45 38.414460962043776 41.0 38.0 41.0 35.0 41.0 46-47 38.1245128940885 40.5 38.0 41.0 34.0 41.0 48-49 37.84995273739317 40.0 37.5 41.0 34.0 41.0 50-51 37.64423159933432 40.0 37.0 41.0 33.0 41.0 52-53 37.46676803719919 40.0 37.0 41.0 33.0 41.0 54-55 37.27179936649565 40.0 36.0 41.0 33.0 41.0 56-57 37.01201712339896 39.5 35.5 41.0 33.0 41.0 58-59 36.79208629966587 39.0 35.0 41.0 32.5 41.0 60-61 36.536376655761345 39.0 35.0 41.0 32.0 41.0 62-63 36.25283112086646 38.5 35.0 41.0 32.0 41.0 64-65 35.92351073940809 37.0 35.0 40.0 31.5 41.0 66-67 35.59682504142581 37.0 35.0 40.0 31.0 41.0 68-69 35.264075553977364 36.0 35.0 39.0 31.0 41.0 70-71 34.91465166731032 36.0 35.0 39.0 31.0 41.0 72-73 34.56377182119841 35.0 35.0 38.5 30.5 40.5 74-75 34.23668716739975 35.0 35.0 37.0 30.0 39.5 76-77 33.92263088332726 35.0 34.0 37.0 30.0 39.0 78-79 33.63104980806855 35.0 34.0 36.0 30.0 39.0 80-81 33.36519659788982 35.0 34.0 36.0 30.0 37.5 82-83 33.12285845362652 35.0 34.0 35.5 29.0 37.0 84-85 32.91565404130678 35.0 34.0 35.0 29.0 37.0 86-87 32.84699685560611 35.0 34.0 35.0 29.0 36.0 88-89 32.71732863890671 35.0 34.0 35.0 29.0 36.0 90-91 32.5968301255704 35.0 34.0 35.0 29.0 36.0 92-93 32.48236090926835 35.0 34.0 35.0 29.0 35.5 94-95 32.41207231129001 35.0 34.0 35.0 29.0 35.0 96-97 32.378984199874274 35.0 34.0 35.0 29.0 35.0 98-99 32.28643502373498 35.0 34.0 35.0 29.0 35.0 100 32.21169181789671 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.202949713870062 1101 2 0.233794838074445 1101 3 0.19879801042736034 1101 4 0.10837825504709997 1101 5 0.1259365392212004 1101 6 0.1349823323405559 1101 7 0.1033247762738867 1101 8 0.04060620319722119 1101 9 0.12782532366791344 1101 10-11 0.15547973639798585 1101 12-13 0.19374983523897527 1101 14-15 0.30631022091189664 1101 16-17 0.27309741678212873 1101 18-19 0.2721571243563119 1101 20-21 0.28708776851201634 1101 22-23 0.27276648356632194 1101 24-25 0.27891058633579746 1101 26-27 0.3073645645772132 1101 28-29 0.27995551821044273 1101 30-31 0.3034414444861895 1101 32-33 0.3429161379311765 1101 34-35 0.3152462772888285 1101 36-37 0.3254253674924428 1101 38-39 0.32460067109185076 1101 40-41 0.3396637676661882 1101 42-43 0.3418589254792792 1101 44-45 0.3247408706104338 1101 46-47 0.4108594914458692 1101 48-49 0.41305793139959945 1101 50-51 0.38038150278782723 1101 52-53 0.4348594628275819 1101 54-55 0.436023603401658 1101 56-57 0.46331765552174886 1101 58-59 0.4254729404012849 1101 60-61 0.4366798448372009 1101 62-63 0.42678611174356007 1101 64-65 0.4660708706956598 1101 66-67 0.48661589723520393 1101 68-69 0.423550150240537 1101 70-71 0.45640881354598406 1101 72-73 0.4232358425780447 1101 74-75 0.4704884901849482 1101 76-77 0.3921059845816899 1101 78-79 0.3632227172112721 1101 80-81 0.3681157615136854 1101 82-83 0.39540010064672515 1101 84-85 0.4666306049902147 1101 86-87 0.45659405164213496 1101 88-89 0.4230178863787373 1101 90-91 0.3692261098734093 1101 92-93 0.3722587529701755 1101 94-95 0.36566329788774254 1101 96-97 0.4052145097894808 1101 98-99 0.396430097622158 1101 100 0.391734982856768 1102 1 0.22248793291209523 1102 2 0.2184162715269693 1102 3 0.21774449431413245 1102 4 0.1054524095897662 1102 5 0.12688063146334372 1102 6 0.13076886041219637 1102 7 0.1282636060062856 1102 8 0.029254017552403866 1102 9 0.1313017154644811 1102 10-11 0.18325815654174704 1102 12-13 0.18168745596121738 1102 14-15 0.24486595713482018 1102 16-17 0.24223004439826212 1102 18-19 0.25583903952203 1102 20-21 0.2398786753216413 1102 22-23 0.23601802621983126 1102 24-25 0.23223318643019297 1102 26-27 0.2664601928417909 1102 28-29 0.23475202316990362 1102 30-31 0.23236004256934706 1102 32-33 0.29438387143925127 1102 34-35 0.25262813225479164 1102 36-37 0.285258447377565 1102 38-39 0.2614787408659751 1102 40-41 0.25796519383010974 1102 42-43 0.2625357900695846 1102 44-45 0.30849260244458776 1102 46-47 0.3585931128315778 1102 48-49 0.3233846316154185 1102 50-51 0.2863255643821532 1102 52-53 0.29447564900139156 1102 54-55 0.2924259242405256 1102 56-57 0.30416741376615164 1102 58-59 0.2689282743576058 1102 60-61 0.29295105336257166 1102 62-63 0.30042441970142164 1102 64-65 0.35048307239251386 1102 66-67 0.3701279120174874 1102 68-69 0.24382012783790685 1102 70-71 0.2288194068822449 1102 72-73 0.2439688809121563 1102 74-75 0.30004554742188816 1102 76-77 0.2930616951347389 1102 78-79 0.2938461932947263 1102 80-81 0.28514358490718195 1102 82-83 0.2719403626911756 1102 84-85 0.26839285014195724 1102 86-87 0.2696492996359581 1102 88-89 0.23739637732825258 1102 90-91 0.2384841010145209 1102 92-93 0.3013293828275039 1102 94-95 0.2656660236865065 1102 96-97 0.26561035799397104 1102 98-99 0.24773090265686903 1102 100 0.28288236860679206 1103 1 0.1765997926048115 1103 2 0.21369521160440996 1103 3 0.22484736553129636 1103 4 0.13404918169968738 1103 5 0.1489605342569149 1103 6 0.12480581964234716 1103 7 0.1429878086399654 1103 8 0.04360670731408334 1103 9 0.08339469717343917 1103 10-11 0.12970255850544987 1103 12-13 0.16144323294391683 1103 14-15 0.1865473062457852 1103 16-17 0.22807440526050726 1103 18-19 0.2562944265413378 1103 20-21 0.2447086554541542 1103 22-23 0.23570139796233747 1103 24-25 0.19043477099469897 1103 26-27 0.22008174402019165 1103 28-29 0.23012560653131686 1103 30-31 0.2525848275184046 1103 32-33 0.2794456936567258 1103 34-35 0.2686945111576122 1103 36-37 0.2722008803543261 1103 38-39 0.27295409381996905 1103 40-41 0.2383287008991104 1103 42-43 0.22288949275570502 1103 44-45 0.16968540185127523 1103 46-47 0.28019636738086007 1103 48-49 0.28602747963291364 1103 50-51 0.27579120384298506 1103 52-53 0.31292887745674847 1103 54-55 0.2792796794094343 1103 56-57 0.2795566074603357 1103 58-59 0.2564943900421781 1103 60-61 0.25301300628429146 1103 62-63 0.2761950240425861 1103 64-65 0.317286962883891 1103 66-67 0.3002388738948767 1103 68-69 0.28489720102965066 1103 70-71 0.2917695238391005 1103 72-73 0.2668110017492822 1103 74-75 0.27637707408732126 1103 76-77 0.27255017633658696 1103 78-79 0.30578232900765556 1103 80-81 0.2929265427439489 1103 82-83 0.3194864808486244 1103 84-85 0.301636515889939 1103 86-87 0.304894840643648 1103 88-89 0.314125917432051 1103 90-91 0.30772313683807795 1103 92-93 0.28834669705218374 1103 94-95 0.2737670173650244 1103 96-97 0.29730842920584877 1103 98-99 0.3413197049353869 1103 100 0.2918484568635762 1104 1 0.1136619035298736 1104 2 0.1329931497591872 1104 3 0.13726475916809733 1104 4 0.06919312708759406 1104 5 0.07617605784167836 1104 6 0.0876296229556317 1104 7 0.06307261622114169 1104 8 0.025600033510571052 1104 9 0.07059407201244738 1104 10-11 0.06929409140540344 1104 12-13 0.12235334337994885 1104 14-15 0.16286053496930464 1104 16-17 0.14756226679086382 1104 18-19 0.16485411382656423 1104 20-21 0.15300067369826564 1104 22-23 0.17877664422901063 1104 24-25 0.17800740576981156 1104 26-27 0.20061919285789998 1104 28-29 0.20499609965450105 1104 30-31 0.24322323162065373 1104 32-33 0.2562708135256031 1104 34-35 0.23731579536259773 1104 36-37 0.20457737539401677 1104 38-39 0.2548510187908519 1104 40-41 0.28477904820167765 1104 42-43 0.2576172109255168 1104 44-45 0.20130686302911016 1104 46-47 0.1846509177938671 1104 48-49 0.17158970583786726 1104 50-51 0.1446730645765939 1104 52-53 0.2084912594080066 1104 54-55 0.19794331789765351 1104 56-57 0.21300139891188508 1104 58-59 0.21421390038174337 1104 60-61 0.21125833569590213 1104 62-63 0.19065694382677378 1104 64-65 0.17869679373086456 1104 66-67 0.16288954383664844 1104 68-69 0.17234904636519843 1104 70-71 0.1984672353691863 1104 72-73 0.200848447197977 1104 74-75 0.20508904768685454 1104 76-77 0.23934726335799184 1104 78-79 0.23043083265219622 1104 80-81 0.24350009179023857 1104 82-83 0.26123831910653195 1104 84-85 0.2719699364312902 1104 86-87 0.2879963347309058 1104 88-89 0.26042025708226646 1104 90-91 0.2415745047612674 1104 92-93 0.22988436820553204 1104 94-95 0.27342216572485967 1104 96-97 0.26110024998362746 1104 98-99 0.28095744115583443 1104 100 0.3301765600178612 1105 1 0.09685285492713547 1105 2 0.12685533269134197 1105 3 0.12344023968932305 1105 4 0.05164503884954286 1105 5 0.043049831099885694 1105 6 0.08764330664546094 1105 7 0.09189773019805614 1105 8 0.052664526105104414 1105 9 0.07251119307325382 1105 10-11 0.08831944631959487 1105 12-13 0.10826069781749226 1105 14-15 0.1039628039377618 1105 16-17 0.12604441372889852 1105 18-19 0.1465186817255244 1105 20-21 0.1393128005092379 1105 22-23 0.12716007076860336 1105 24-25 0.14497921203171416 1105 26-27 0.08958111604472663 1105 28-29 0.119731734177833 1105 30-31 0.12511196123210766 1105 32-33 0.18856616341052757 1105 34-35 0.17002008091366605 1105 36-37 0.15456991958883037 1105 38-39 0.19204682504889803 1105 40-41 0.214328691484738 1105 42-43 0.16434988327109323 1105 44-45 0.16994751765682992 1105 46-47 0.1881463381116717 1105 48-49 0.2151263413491975 1105 50-51 0.19725810018167067 1105 52-53 0.2067981134639112 1105 54-55 0.2574949366881967 1105 56-57 0.2639908801672135 1105 58-59 0.26467194484443013 1105 60-61 0.3093094672708432 1105 62-63 0.29429755170523464 1105 64-65 0.26824792616855575 1105 66-67 0.29199398365628326 1105 68-69 0.28330053672166855 1105 70-71 0.23848200612986403 1105 72-73 0.3024858692245189 1105 74-75 0.27415890669549725 1105 76-77 0.26436776983188537 1105 78-79 0.3027970764901724 1105 80-81 0.27866404466553973 1105 82-83 0.2956209874653766 1105 84-85 0.2786624144427847 1105 86-87 0.2984944334805206 1105 88-89 0.2654910172080278 1105 90-91 0.30051637672775655 1105 92-93 0.30562866763452234 1105 94-95 0.2736633457741604 1105 96-97 0.29210265570470995 1105 98-99 0.282028721582833 1105 100 0.3173717563284484 1106 1 0.09020486995886046 1106 2 0.12640701269663168 1106 3 0.08816020785509693 1106 4 0.027725881849669065 1106 5 0.042561084169562946 1106 6 0.06827212328224164 1106 7 0.05284455545305633 1106 8 0.0535033000886358 1106 9 0.08687570240613951 1106 10-11 0.06618711457773685 1106 12-13 0.05461806105724776 1106 14-15 0.10364740967020225 1106 16-17 0.10417038406028212 1106 18-19 0.14824913198042822 1106 20-21 0.12741008772218976 1106 22-23 0.1478136108734489 1106 24-25 0.11280203243669007 1106 26-27 0.12119930171859039 1106 28-29 0.08024614975872169 1106 30-31 0.160496325512689 1106 32-33 0.15041814058210434 1106 34-35 0.16446581252758818 1106 36-37 0.19966169326230698 1106 38-39 0.17765747825230704 1106 40-41 0.19685338418906895 1106 42-43 0.18085735991387253 1106 44-45 0.17127479321580807 1106 46-47 0.18880928701013744 1106 48-49 0.1990227754669931 1106 50-51 0.18254380915046653 1106 52-53 0.19700835919702087 1106 54-55 0.20692212362499163 1106 56-57 0.2230523418678132 1106 58-59 0.17121968535619914 1106 60-61 0.15660358791100748 1106 62-63 0.2155706808383826 1106 64-65 0.23063322936566522 1106 66-67 0.2452158103664317 1106 68-69 0.2598960587760857 1106 70-71 0.3178072786870558 1106 72-73 0.3196071105481124 1106 74-75 0.32705004031734575 1106 76-77 0.3341691395764883 1106 78-79 0.22857439284977943 1106 80-81 0.2894608674934247 1106 82-83 0.30742752113761185 1106 84-85 0.3215246294055234 1106 86-87 0.2887436151464797 1106 88-89 0.2816106255249977 1106 90-91 0.2819936305558457 1106 92-93 0.2754016064100213 1106 94-95 0.2923683717519623 1106 96-97 0.3330067310795428 1106 98-99 0.3110369541073936 1106 100 0.3065349595278164 1107 1 0.08278327948784181 1107 2 0.07616493676997038 1107 3 0.060729602828430984 1107 4 0.03654474280428133 1107 5 0.07061937322254863 1107 6 0.07855845209045498 1107 7 0.05479471772547129 1107 8 0.025349595740578934 1107 9 0.05627611060985771 1107 10-11 0.06999169177652931 1107 12-13 0.06228547693859099 1107 14-15 0.08424347275184374 1107 16-17 0.094308874084156 1107 18-19 0.10362894821372493 1107 20-21 0.08578024730301337 1107 22-23 0.11878666631924517 1107 24-25 0.09071212624414215 1107 26-27 0.11643271901235153 1107 28-29 0.13423017089166933 1107 30-31 0.13474864454769886 1107 32-33 0.10883901421789233 1107 34-35 0.09797977620422671 1107 36-37 0.10055888242033717 1107 38-39 0.09130619646914795 1107 40-41 0.053807333816898506 1107 42-43 0.029439227654989963 1107 44-45 0.05992362720177624 1107 46-47 0.025205566186961903 1107 48-49 -0.03492486652060478 1107 50-51 0.008120386942707114 1107 52-53 0.013639340256140997 1107 54-55 0.0593918415636665 1107 56-57 0.05552538498285031 1107 58-59 -0.017219554467494902 1107 60-61 0.018404261195499316 1107 62-63 0.02565962060106841 1107 64-65 -0.003007400089536816 1107 66-67 -0.06937701491312964 1107 68-69 -0.05254407108778736 1107 70-71 -0.06217918906923359 1107 72-73 -0.07007386349487632 1107 74-75 -0.06008779124047692 1107 76-77 -0.06835325062045428 1107 78-79 -0.06127531334254854 1107 80-81 -0.06645756772159217 1107 82-83 -0.061674109397777954 1107 84-85 -0.04287920682062207 1107 86-87 -0.08771791617423474 1107 88-89 -0.08132699028225687 1107 90-91 -0.053457559764325424 1107 92-93 -0.08055207107960172 1107 94-95 -0.06314724973851327 1107 96-97 -0.02108645584839053 1107 98-99 0.0016686008479496195 1107 100 0.020735727739406684 1108 1 0.0428307075037182 1108 2 0.0529004170259455 1108 3 0.03361519632252197 1108 4 0.012242107038183292 1108 5 3.85150464538242E-4 1108 6 0.00597406991248306 1108 7 0.06072596554618315 1108 8 0.03474059708985067 1108 9 0.06354812085669437 1108 10-11 0.04587473670294884 1108 12-13 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2214 54-55 -0.18768039882402832 2214 56-57 -0.17818047230918665 2214 58-59 -0.19767634253934574 2214 60-61 -0.24165018839688202 2214 62-63 -0.26306329209166535 2214 64-65 -0.2533133138819821 2214 66-67 -0.29122023886519344 2214 68-69 -0.3038009591912214 2214 70-71 -0.2901572464394917 2214 72-73 -0.2695309151037506 2214 74-75 -0.30067464008664047 2214 76-77 -0.24226922457112465 2214 78-79 -0.27593928862749806 2214 80-81 -0.2781480347345635 2214 82-83 -0.3032096797242474 2214 84-85 -0.2752825672312156 2214 86-87 -0.24708739922380119 2214 88-89 -0.26433195808312604 2214 90-91 -0.25869385092887853 2214 92-93 -0.25403465488734156 2214 94-95 -0.29490719876151417 2214 96-97 -0.3030605504158572 2214 98-99 -0.2993541857539981 2214 100 -0.3107997141904413 2215 1 -0.19550652750734088 2215 2 -0.17877470327578493 2215 3 -0.1947890174894198 2215 4 -0.13696455182513034 2215 5 -0.14847686020004858 2215 6 -0.13066918668785377 2215 7 -0.1490054689706639 2215 8 -0.09493816477775141 2215 9 -0.15612608905356495 2215 10-11 -0.18630597600351706 2215 12-13 -0.18473207939366176 2215 14-15 -0.2858741754467644 2215 16-17 -0.24008744516394387 2215 18-19 -0.2403141556265922 2215 20-21 -0.23753234249036126 2215 22-23 -0.25166877736047155 2215 24-25 -0.2561419142511312 2215 26-27 -0.26619847833332244 2215 28-29 -0.24861034724139586 2215 30-31 -0.28265279146243216 2215 32-33 -0.2774735903926313 2215 34-35 -0.27932761789728744 2215 36-37 -0.2553891005376627 2215 38-39 -0.286773214070287 2215 40-41 -0.32141058519367505 2215 42-43 -0.3302362310926341 2215 44-45 -0.28988971940621155 2215 46-47 -0.2453419219350863 2215 48-49 -0.2982204621787261 2215 50-51 -0.22960455596850693 2215 52-53 -0.23318574267717196 2215 54-55 -0.2847629623569503 2215 56-57 -0.3401682159444732 2215 58-59 -0.28600524029938157 2215 60-61 -0.2217403300020777 2215 62-63 -0.2499636330406716 2215 64-65 -0.3340195099781127 2215 66-67 -0.2959884444663885 2215 68-69 -0.32025559165257533 2215 70-71 -0.40278937369666323 2215 72-73 -0.34751929932719605 2215 74-75 -0.3657335185148156 2215 76-77 -0.341685473142185 2215 78-79 -0.3341166749135738 2215 80-81 -0.35466412510984924 2215 82-83 -0.33546900466045315 2215 84-85 -0.34819785967921746 2215 86-87 -0.4940569793768006 2215 88-89 -0.47069566129187734 2215 90-91 -0.5178210305229598 2215 92-93 -0.4278597871692611 2215 94-95 -0.43165710143589564 2215 96-97 -0.42794543729446133 2215 98-99 -0.44892754737601237 2215 100 -0.46193563976418517 2216 1 -0.2663224196990086 2216 2 -0.26612700550648327 2216 3 -0.25256776667255565 2216 4 -0.14676647344154503 2216 5 -0.20877110771413498 2216 6 -0.17712002474575428 2216 7 -0.1873419814488031 2216 8 -0.16881354230119427 2216 9 -0.2505082752454797 2216 10-11 -0.2935369850287586 2216 12-13 -0.3195322348185172 2216 14-15 -0.4120985877465202 2216 16-17 -0.4091128606605139 2216 18-19 -0.36609370842688804 2216 20-21 -0.3480716490835505 2216 22-23 -0.4112744309396561 2216 24-25 -0.42921720699738586 2216 26-27 -0.45899241676388414 2216 28-29 -0.4166058522838654 2216 30-31 -0.4267919055407461 2216 32-33 -0.5467286107921439 2216 34-35 -0.5363669951381453 2216 36-37 -0.5233174903008191 2216 38-39 -0.5054235346340619 2216 40-41 -0.41210474263199615 2216 42-43 -0.44102655766145205 2216 44-45 -0.4162165708165091 2216 46-47 -0.3965733990010918 2216 48-49 -0.5006118855525017 2216 50-51 -0.41928889338174713 2216 52-53 -0.4069979788523099 2216 54-55 -0.3931965270613844 2216 56-57 -0.49231855417015424 2216 58-59 -0.4465505077890981 2216 60-61 -0.44969428538808387 2216 62-63 -0.39851645983026884 2216 64-65 -0.4679654910092097 2216 66-67 -0.46995720936862995 2216 68-69 -0.46325396675633357 2216 70-71 -0.46313351612234754 2216 72-73 -0.42422831043653986 2216 74-75 -0.4874486229850419 2216 76-77 -0.44051470891108835 2216 78-79 -0.431994154204979 2216 80-81 -0.4765044084069743 2216 82-83 -0.4607677118082236 2216 84-85 -0.4838811108846457 2216 86-87 -0.40284532781107174 2216 88-89 -0.40736687590180765 2216 90-91 -0.46800824682843256 2216 92-93 -0.4632544868283901 2216 94-95 -0.4651734563697545 2216 96-97 -0.3924385844712077 2216 98-99 -0.42703339523627193 2216 100 -0.4544469875449657 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22.0 3 10.0 4 21.0 5 47.0 6 156.0 7 347.0 8 707.0 9 1234.0 10 2017.0 11 3463.0 12 5750.0 13 8829.0 14 9780.0 15 12978.0 16 17186.0 17 27091.0 18 32793.0 19 17163.0 20 10990.0 21 10486.0 22 11273.0 23 11589.0 24 13003.0 25 15630.0 26 19468.0 27 25444.0 28 31758.0 29 40544.0 30 50986.0 31 64790.0 32 82729.0 33 107343.0 34 152453.0 35 253409.0 36 551466.0 37 1214153.0 38 1696249.0 39 511559.0 40 677.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.423108670357045 18.425196567809216 13.042041864816218 24.10965289701753 2 16.813964852998556 19.937602219332106 38.177767879697655 25.070665047971687 3 18.778121085328596 24.889609132490374 29.854970116159883 26.477299666021146 4 13.03725176902982 16.503507351989626 36.85324754333816 33.60599333564239 5 13.384770532378598 37.7505749101896 34.37855268976622 14.486101867665582 6 31.429451571353944 36.52717020708136 17.35324259982215 14.690135621742545 7 28.86670454885307 32.374555133534706 21.176879903500115 17.58186041411211 8 27.252437690163788 33.40238458405539 19.630213782033366 19.714963943747456 9 27.49425583448509 15.18914075578145 19.786985367829963 37.5296180419035 10-11 25.689414990410903 25.00925613382107 27.385435779976564 21.915893095791464 12-13 26.44927702380599 23.096658476864224 27.450934456923985 23.003130042405804 14-15 23.68935974142986 24.117674854548316 25.573637202295323 26.6193282017265 16-17 22.822984241344944 27.58948941830009 26.02171866816147 23.565807672193497 18-19 22.81952697052747 26.29496334027569 28.43820243536847 22.447307253828367 20-21 24.118643598075042 24.939493694962888 28.18740475951697 22.754457947445097 22-23 23.862257591570426 24.893434547294085 28.12145635414612 23.122851506989374 24-25 23.411776997009714 25.126017480750956 28.033172274997835 23.42903324724149 26-27 23.090968508876205 25.489879206064302 28.183447191185085 23.235705093874408 28-29 22.98119239361134 25.567055080109462 28.38054434775344 23.071208178525758 30-31 23.146959654486114 25.324427751102863 28.11083037033389 23.417782224077136 32-33 23.33742292140164 25.225715848242213 28.12514239194539 23.31171883841075 34-35 23.663374965374217 25.04242343987538 27.981092976186034 23.313108618564364 36-37 23.82354759701302 25.067783459918303 27.684801426474344 23.42386751659433 38-39 23.393037744356267 25.322017762226 27.869587032384235 23.415357461033494 40-41 23.164444481681173 25.271827298439657 27.928704561755435 23.63502365812373 42-43 23.45068999933661 25.323721342719207 27.5018218256963 23.723766832247886 44-45 23.24161038662951 25.37082951826617 27.45483168381056 23.932728411293763 46-47 23.844714637142566 25.366681813150933 27.149928337801267 23.638675211905237 48-49 23.854798173724877 25.437404948036345 26.788428986833267 23.91936789140551 50-51 23.370357108069744 25.784137960217357 26.78210723142244 24.063397700290455 52-53 23.66337039760707 25.698494120215905 26.528640576672608 24.109494905504413 54-55 23.366427641132354 25.901405709666314 26.696996462022888 24.03517018717845 56-57 23.579678206843084 25.69658404724569 26.593970756327174 24.129766989584056 58-59 23.741034256536132 25.16510637660987 26.7677824424339 24.326076924420097 60-61 23.684166675397464 25.547079199301137 26.924739036489242 23.844015088812156 62-63 23.293986549414566 25.515083266431628 26.982320987299552 24.20860919685425 64-65 23.507920549765558 25.750277758809155 26.67409682903864 24.067704862386645 66-67 23.311413357444806 25.47624233826811 26.637760144987855 24.574584159299224 68-69 23.49470117227473 25.636233659825297 26.53658627035042 24.33247889754955 70-71 23.59346143118072 25.500404438717418 26.656837985059795 24.24929614504207 72-73 23.520917666166294 25.398950831935522 26.62602408130006 24.454107420598124 74-75 23.536200006659232 25.579258524365912 26.5865771006539 24.297964368320955 76-77 23.522048253545442 25.355381919176008 26.460805209357225 24.66176461792132 78-79 23.394329462437284 25.48199948101834 26.500972018953302 24.62269903759108 80-81 23.27394605669274 25.732863823041026 26.523380494042513 24.469809626223725 82-83 23.259241640111117 25.44314354431495 26.65018072660289 24.647434088971043 84-85 23.302080132897547 25.53117846683333 26.3910468811963 24.775694519072818 86-87 23.431360517844002 25.488865709931552 26.38488648685507 24.694887285369376 88-89 22.96002197950746 25.56139557909642 26.41671024752666 25.061872193869466 90-91 23.10017062825411 25.421346914475727 26.369432301176616 25.10905015609355 92-93 23.068929470703793 25.383941173315694 26.586511899898458 24.96061745608206 94-95 23.048621834715867 25.123730322427573 26.585106902639694 25.242540940216866 96-97 23.0268736563122 25.183772328669402 26.602022776623485 25.18733123839491 98-99 22.710352308396725 25.233893150987274 26.61940271650911 25.43635182410689 100 22.775213220051945 24.90493148068434 26.522088215690548 25.79776708357317 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 86.0 1 93.5 2 341.0 3 586.0 4 378.5 5 242.0 6 602.5 7 1422.0 8 2480.5 9 2937.5 10 2294.5 11 1628.0 12 1700.0 13 2001.0 14 2360.0 15 2738.5 16 3037.0 17 3380.5 18 3762.5 19 4049.0 20 4441.5 21 4917.0 22 5644.5 23 6853.5 24 8547.0 25 10415.5 26 12702.5 27 15960.5 28 21754.5 29 28128.5 30 32889.0 31 39734.5 32 46948.5 33 53161.0 34 62409.0 35 74114.0 36 86762.5 37 101106.5 38 116017.0 39 128407.5 40 146385.5 41 164240.0 42 174400.5 43 189387.0 44 196446.5 45 193498.5 46 195932.0 47 202634.0 48 208309.5 49 206893.5 50 201097.0 51 193674.0 52 183361.0 53 177351.0 54 171175.0 55 159920.0 56 145793.5 57 129373.5 58 117736.0 59 112888.0 60 104957.0 61 95767.5 62 89960.0 63 80463.0 64 66469.5 65 52797.5 66 43125.0 67 33495.0 68 23956.0 69 16907.5 70 11100.5 71 7527.0 72 6052.5 73 4465.0 74 3459.0 75 2779.5 76 1790.0 77 1284.5 78 908.0 79 429.0 80 206.5 81 102.5 82 30.0 83 20.0 84 12.0 85 5.5 86 5.0 87 9.0 88 8.5 89 4.0 90 2.0 91 2.0 92 1.5 93 0.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.1869426008051295E-4 2 5.981346572578756E-4 3 1.1962693145157511E-4 4 0.011085428981179294 5 5.383211915320881E-4 6 0.003449243190187082 7 8.573263420696217E-4 8 0.1346401113487478 9 0.0013757097116931139 10-11 0.0 12-13 1.1962693145157511E-4 14-15 1.9937821908595855E-4 16-17 0.0 18-19 1.3956475336017097E-4 20-21 0.0 22-23 2.6916059576604403E-4 24-25 3.788186162633212E-4 26-27 0.005482901024863859 28-29 0.007207522619957401 30-31 0.013677345829296753 32-33 0.016458671985545875 34-35 0.02524128253628235 36-37 0.0365460275584562 38-39 0.04164014105610244 40-41 0.05226700013338403 42-43 0.06879545449560999 44-45 0.1057801141360553 46-47 0.11223996843444034 48-49 0.10974774069586588 50-51 0.1110337302089703 52-53 0.10631843532758739 54-55 0.11300757457792131 56-57 0.09501369030541354 58-59 0.08982985660917861 60-61 0.09162426058095224 62-63 0.0899195768077673 64-65 9.968910954297926E-6 66-67 9.968910954297926E-6 68-69 4.984455477148964E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 5.981346572578756E-5 78-79 2.990673286289378E-5 80-81 2.492227738574482E-4 82-83 1.1962693145157511E-4 84-85 0.0 86-87 5.682279243949818E-4 88-89 2.3925386290315023E-4 90-91 2.193160409945544E-4 92-93 9.171398077954092E-4 94-95 9.570154516126009E-4 96-97 2.3925386290315023E-4 98-99 2.990673286289378E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5015593.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.570311286077864 #Duplication Level Percentage of deduplicated Percentage of total 1 59.13928433532249 10.982349193428071 2 12.314960476024131 4.573852990310275 3 5.755981183415076 3.2067108699847453 4 3.6091315983780135 2.6809078901719787 5 2.6029377512526803 2.4168682149522893 6 1.9717691586669892 2.196982023644031 7 1.5727444826888883 2.044444823189591 8 1.303512530350123 1.9365306763123844 9 1.0342780909263596 1.7286179494385558 >10 9.18682945436544 34.61898898787234 >50 0.9954225877990099 12.714362838230903 >100 0.4867780327327981 16.015185669474487 >500 0.019912281062230457 2.5184029851974223 >1k 0.006027501214723522 1.6607995642712963 >5k 2.1526790052583985E-4 0.26180229501534996 >10k+ 2.1526790052583985E-4 0.4431930285062875 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11904 0.23733983199992503 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10269 0.20474149317937082 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7799 0.15549507306513904 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5299 0.10565051829364942 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.990673286289378E-5 0.0 12-13 0.0 0.0 0.0 5.981346572578756E-5 0.0 14-15 0.0 0.0 0.0 1.2959584240587304E-4 0.0 16-17 0.0 0.0 0.0 1.495336643144689E-4 0.0 18-19 0.0 0.0 0.0 1.9937821908595852E-4 0.0 20-21 0.0 0.0 0.0 2.1931604099455438E-4 0.0 22-23 0.0 0.0 0.0 3.688497053090233E-4 0.0 24-25 0.0 0.0 0.0 8.473574311153238E-4 0.0 26-27 0.0 0.0 0.0 0.0018342796155908186 0.0 28-29 0.0 0.0 0.0 0.00553274557963535 0.0 30-31 0.0 0.0 0.0 0.017834381697238992 0.0 32-33 0.0 0.0 0.0 0.04388314602081947 0.0 34-35 0.0 0.0 0.0 0.0817650076471516 0.0 36-37 0.0 0.0 0.0 0.14416042131010232 0.0 38-39 0.0 0.0 0.0 0.2419454688608107 0.0 40-41 0.0 0.0 0.0 0.35007824598208026 0.0 42-43 0.0 0.0 0.0 0.46271697085469254 0.0 44-45 0.0 0.0 0.0 0.5968686055666798 0.0 46-47 0.0 0.0 0.0 0.7627512838461972 0.0 48-49 0.0 0.0 0.0 0.9361804277181183 0.0 50-51 0.0 0.0 0.0 1.1175547936206147 0.0 52-53 0.0 0.0 0.0 1.3027472524186074 0.0 54-55 0.0 0.0 0.0 1.513200931574791 0.0 56-57 0.0 0.0 0.0 1.7603701097756537 0.0 58-59 0.0 0.0 0.0 2.028852819596805 0.0 60-61 0.0 0.0 0.0 2.31128203584302 0.0 62-63 0.0 0.0 0.0 2.596432764779758 0.0 64-65 0.0 0.0 0.0 2.9059475120888 0.0 66-67 0.0 0.0 0.0 3.2586077060080436 0.0 68-69 0.0 0.0 0.0 3.6544631910922596 0.0 70-71 0.0 0.0 0.0 4.064005990916727 0.0 72-73 0.0 0.0 0.0 4.487904022515384 0.0 74-75 0.0 0.0 0.0 4.933643938014907 0.0 76-77 0.0 0.0 0.0 5.403339146537608 0.0 78-79 0.0 0.0 0.0 5.934612318025007 0.0 80-81 0.0 0.0 0.0 6.489641404316499 0.0 82-83 0.0 0.0 0.0 7.061946613291788 0.0 84-85 0.0 0.0 0.0 7.641479282708944 0.0 86-87 0.0 0.0 0.0 8.253949233919101 0.0 88 0.0 0.0 0.0 8.748457061807049 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 18575 0.0 29.288187 1 GTATCAA 27065 0.0 29.196495 1 GTACATG 24990 0.0 27.146492 1 TACATGG 24200 0.0 26.74725 2 TCAACGC 31380 0.0 24.839907 4 CAACGCA 31520 0.0 24.757696 5 ATCAACG 31720 0.0 24.603312 3 AACGCAG 31840 0.0 24.508873 6 TATCAAC 33075 0.0 23.723143 2 ACATGGG 26660 0.0 23.59129 3 ACGCAGA 35855 0.0 21.672132 7 CGCAGAG 36560 0.0 21.215414 8 GTACTTT 17175 0.0 20.923285 14-15 AGTACTT 16995 0.0 20.44016 12-13 AGAGTAC 28335 0.0 20.399893 10-11 GCAGAGT 37930 0.0 20.27401 9 GAGTACA 17645 0.0 20.261696 1 CATGGGG 17710 0.0 19.841862 4 GTATAGA 1255 0.0 19.840149 1 GAGTACT 18280 0.0 19.761389 12-13 >>END_MODULE