FastQCFastQC Report
Wed 25 May 2016
SRR1032998_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032998_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3906515
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36850.033.5441781
GTATCAA56950.032.6813771
TCAACGC58950.029.5793764
ATCAACG60750.028.8576853
GTACATG129550.028.0803491
CAACGCA62300.027.9884745
AACGCAG63700.027.4471266
TACATGG130700.026.6465842
TATCAAC71150.025.101962
ACATGGG132950.024.8876113
ACGCAGA77000.022.2179537
CGCAGAG81100.020.9788238
CATGGGG92550.020.9227944
GAGTACA94500.019.4466081
GTATTAG21500.017.9256271
GTATAGA16850.017.5727441
AGTACAT94350.017.484942
GTCTAGC5700.017.3158321
GCAGAGT96350.017.2681129
GTATATA28100.016.893281
Produced by FastQC (version 0.11.5)