FastQCFastQC Report
Wed 25 May 2016
SRR1032998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032998_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3906515
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92680.23724470531919115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74850.1916030016523679No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40820.10449211125517245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62150.050.948431
GTATCAA111350.041.4317551
ATCAACG132400.034.3914263
TCAACGC133300.034.159224
CAACGCA137300.033.1615035
TATCAAC141650.032.4444772
AACGCAG142000.032.1959886
ACGCAGA164800.027.6264637
CGCAGAG167100.027.133628
GTACATG123550.026.9597281
TACATGG125450.026.0226542
ACACCGT7500.025.7126246
GCAGAGT184950.024.2549259
ACATGGG127450.023.8057923
GAGTACT115300.021.490112-13
TACACCG7250.020.760675
CATGGGG89950.020.3951194
CAGAGTA181400.020.34015310-11
AGAGTAC166350.020.1755210-11
GTGGTAT32900.020.1342411
Produced by FastQC (version 0.11.5)